LOCUS       NM_017165                877 bp    mRNA    linear   ROD 15-OCT-2024
DEFINITION  Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript
            variant 1, mRNA.
ACCESSION   NM_017165
VERSION     NM_017165.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Chen,B., Fan,P., Song,X. and Duan,M.
  TITLE     The role and possible mechanism of the ferroptosis-related
            SLC7A11/GSH/GPX4 pathway in myocardial ischemia-reperfusion injury
  JOURNAL   BMC Cardiovasc Disord 24 (1), 531 (2024)
   PUBMED   39354361
  REMARK    GeneRIF: The role and possible mechanism of the ferroptosis-related
            SLC7A11/GSH/GPX4 pathway in myocardial ischemia-reperfusion injury.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  AUTHORS   Liu,D., Ren,L. and Liu,J.
  TITLE     METTL14 promotes chondrocyte ferroptosis in osteoarthritis via m6A
            modification of GPX4
  JOURNAL   Int J Rheum Dis 27 (8), e15297 (2024)
   PUBMED   39175261
  REMARK    GeneRIF: METTL14 promotes chondrocyte ferroptosis in osteoarthritis
            via m6A modification of GPX4.
REFERENCE   3  (bases 1 to 877)
  AUTHORS   Chen,D., Tang,Q., Song,W. and He,Y.
  TITLE     Platelet-derived exosomes alleviate tendon stem/progenitor cell
            senescence and ferroptosis by regulating AMPK/Nrf2/GPX4 signaling
            and improve tendon-bone junction regeneration in rats
  JOURNAL   J Orthop Surg Res 19 (1), 382 (2024)
   PUBMED   38943181
  REMARK    GeneRIF: Platelet-derived exosomes alleviate tendon stem/progenitor
            cell senescence and ferroptosis by regulating AMPK/Nrf2/GPX4
            signaling and improve tendon-bone junction regeneration in rats.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 877)
  AUTHORS   Pfeifer,H., Conrad,M., Roethlein,D., Kyriakopoulos,A.,
            Brielmeier,M., Bornkamm,G.W. and Behne,D.
  TITLE     Identification of a specific sperm nuclei selenoenzyme necessary
            for protamine thiol cross-linking during sperm maturation
  JOURNAL   FASEB J 15 (7), 1236-1238 (2001)
   PUBMED   11344099
REFERENCE   5  (bases 1 to 877)
  AUTHORS   Ursini,F., Heim,S., Kiess,M., Maiorino,M., Roveri,A., Wissing,J.
            and Flohe,L.
  TITLE     Dual function of the selenoprotein PHGPx during sperm maturation
  JOURNAL   Science 285 (5432), 1393-1396 (1999)
   PUBMED   10464096
  REMARK    GeneRIF: Rat Gpx4 is a moonlighting protein that functions both as
            a peroxidase, as well as a structural protein.
REFERENCE   6  (bases 1 to 877)
  AUTHORS   Arai,M., Imai,H., Koumura,T., Yoshida,M., Emoto,K., Umeda,M.,
            Chiba,N. and Nakagawa,Y.
  TITLE     Mitochondrial phospholipid hydroperoxide glutathione peroxidase
            plays a major role in preventing oxidative injury to cells
  JOURNAL   J Biol Chem 274 (8), 4924-4933 (1999)
   PUBMED   9988735
REFERENCE   7  (bases 1 to 877)
  AUTHORS   Pushpa-Rekha,T.R., Burdsall,A.L., Oleksa,L.M., Chisolm,G.M. and
            Driscoll,D.M.
  TITLE     Rat phospholipid-hydroperoxide glutathione peroxidase. cDNA cloning
            and identification of multiple transcription and translation start
            sites
  JOURNAL   J Biol Chem 270 (45), 26993-26999 (1995)
   PUBMED   7592947
REFERENCE   8  (bases 1 to 877)
  AUTHORS   Imai,H., Sumi,D., Hanamoto,A., Arai,M. and Sugiyama,A.
  TITLE     Molecular cloning and functional expression of a cDNA for rat
            phospholipid hydroperoxide glutathione peroxidase: 3'-untranslated
            region of the gene is necessary for functional expression
  JOURNAL   J Biochem 118 (5), 1061-1067 (1995)
   PUBMED   8749327
REFERENCE   9  (bases 1 to 877)
  AUTHORS   Roveri,A., Casasco,A., Maiorino,M., Dalan,P., Calligaro,A. and
            Ursini,F.
  TITLE     Phospholipid hydroperoxide glutathione peroxidase of rat testis.
            Gonadotropin dependence and immunocytochemical identification
  JOURNAL   J Biol Chem 267 (9), 6142-6146 (1992)
   PUBMED   1556123
REFERENCE   10 (bases 1 to 877)
  AUTHORS   Thomas,J.P., Geiger,P.G., Maiorino,M., Ursini,F. and Girotti,A.W.
  TITLE     Enzymatic reduction of phospholipid and cholesterol hydroperoxides
            in artificial bilayers and lipoproteins
  JOURNAL   Biochim Biophys Acta 1045 (3), 252-260 (1990)
   PUBMED   2386798
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JAXUCZ010000007.1.
            
            On Jan 7, 2019 this sequence version replaced NM_017165.3.
            
            Summary: The protein encoded by this gene belongs to the
            glutathione peroxidase family, members of which catalyze the
            reduction of hydrogen peroxide, organic hydroperoxides and lipid
            hydroperoxides, and thereby protect cells against oxidative damage.
            Several isozymes of this gene family exist in vertebrates, which
            vary in cellular location and substrate specificity. This isozyme
            has a high preference for lipid hydroperoxides and protects cells
            against membrane lipid peroxidation and cell death. It is also
            required for normal sperm development; thus, it has been identified
            as a 'moonlighting' protein because of its ability to serve dual
            functions as a peroxidase, as well as a structural protein in
            mature spermatozoa. Disruption of this gene in mouse spermatocytes
            is associated with male infertility. This isozyme is also a
            selenoprotein, containing the rare amino acid selenocysteine (Sec)
            at its active site. Sec is encoded by the UGA codon, which normally
            signals translation termination. The 3' UTRs of selenoprotein mRNAs
            contain a conserved stem-loop structure, designated the Sec
            insertion sequence (SECIS) element, that is necessary for the
            recognition of UGA as a Sec codon, rather than as a stop signal.
            Transcript variants resulting from alternative splicing or use of
            alternate promoters have been described to encode isoforms with
            different subcellular localization. Pseudogenes of this locus have
            been identified on chromosomes 1, 10 and 14. [provided by RefSeq,
            Jan 2019].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes the canonical isoform A (also known as phGPx
            or the L-form), which is localized to the mitochondria
            (PMID:9988735).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ221899.1, FQ234630.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 9988735
            multifunctional gene product(s)    :: PMID: 10464096
            protein contains selenocysteine    :: PMID: 8749327
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-150               JAXUCZ010000007.1  10303480-10303629   c
            151-245             JAXUCZ010000007.1  10302587-10302681   c
            246-390             JAXUCZ010000007.1  10302351-10302495   c
            391-542             JAXUCZ010000007.1  10302061-10302212   c
            543-567             JAXUCZ010000007.1  10301309-10301333   c
            568-627             JAXUCZ010000007.1  10301165-10301224   c
            628-877             JAXUCZ010000007.1  10300833-10301082   c
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..877
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /note="glutathione peroxidase 4"
                     /db_xref="GeneID:29328"
                     /db_xref="RGD:69226"
     exon            1..150
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..660
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /EC_number="1.11.1.9"
                     /EC_number="1.11.1.12"
                     /note="UGA stop codon recoded as selenocysteine; isoform A
                     precursor is encoded by transcript variant 1; phospholipid
                     hydroperoxide glutathione peroxidase, nuclear;
                     phospholipid hydroperoxide glutathione peroxidase,
                     mitochondrial"
                     /codon_start=1
                     /transl_except=(pos:283..285,aa:Sec)
                     /product="phospholipid hydroperoxide glutathione
                     peroxidase isoform A precursor"
                     /protein_id="NP_058861.3"
                     /db_xref="GeneID:29328"
                     /db_xref="RGD:69226"
                     /translation="MSWGRLSRLLKPALLCGALAVPGLAGTMCASRDDWRCARSMHEF
                     AAKDIDGHMVCLDKYRGCVCIVTNVASQUGKTDVNYTQLVDLHARYAECGLRILAFPC
                     NQFGRQEPGSNQEIKEFAAGYNVRFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNA
                     IKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL"
     exon            151..245
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     exon            246..390
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     exon            391..542
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     exon            543..567
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     exon            568..627
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     exon            628..877
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     regulatory      702..799
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
ORIGIN      
        1 gactcggcct cgcgcgtcca ttggtcggct gcgtgagggg aggagccgct ggctccggcc
       61 gccgagatga gctggggccg tctgagccgc ttattgaagc cagcactgct gtgcggggct
      121 ctggctgtgc ctggcctggc tggcaccatg tgtgcatccc gcgatgattg gcgctgtgcg
      181 cgctccatgc acgaattcgc agccaaggac atcgatgggc acatggtttg cctggataag
      241 tacaggggtt gcgtgtgcat cgtcaccaac gtggcctcgc aatgaggcaa aaccgacgta
      301 aactacactc agctagtcga tctgcatgcc cgatacgccg agtgtggttt acgaatcctg
      361 gccttccctt gcaaccagtt cgggaggcag gagccaggaa gtaatcaaga aatcaaggag
      421 tttgcagccg gctacaatgt caggtttgac atgtacagca agatctgtgt aaatggggac
      481 gatgcccacc cactgtggaa atggatgaaa gtccagccca agggcagggg catgctggga
      541 aatgccatca aatggaactt taccaagttt ctcattgata agaacggctg cgtggtgaag
      601 cgctatggtc ccatggagga gccccaggtg atagagaagg acctgccgtg ctatctctag
      661 ccctacaagt gtgtgcccct gcaccgagcc cccctgccct gtgacccctg gagccttcca
      721 ccccggcact catgacggtc tgcctgaaaa ccagcccgct ggtggggcag tcccgaggac
      781 ctggcgtgca tccccgccgg aggaaggtcc agaggcctgt ggccccgggc tcgagcttca
      841 ccttggctgc cttgtgggaa taaaatgtag aaatgtg
//
