LOCUS       NM_001368043             785 bp    mRNA    linear   ROD 15-OCT-2024
DEFINITION  Rattus norvegicus glutathione peroxidase 4 (Gpx4), transcript
            variant 3, mRNA.
ACCESSION   NM_001368043
VERSION     NM_001368043.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Chen,B., Fan,P., Song,X. and Duan,M.
  TITLE     The role and possible mechanism of the ferroptosis-related
            SLC7A11/GSH/GPX4 pathway in myocardial ischemia-reperfusion injury
  JOURNAL   BMC Cardiovasc Disord 24 (1), 531 (2024)
   PUBMED   39354361
  REMARK    GeneRIF: The role and possible mechanism of the ferroptosis-related
            SLC7A11/GSH/GPX4 pathway in myocardial ischemia-reperfusion injury.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  AUTHORS   Liu,D., Ren,L. and Liu,J.
  TITLE     METTL14 promotes chondrocyte ferroptosis in osteoarthritis via m6A
            modification of GPX4
  JOURNAL   Int J Rheum Dis 27 (8), e15297 (2024)
   PUBMED   39175261
  REMARK    GeneRIF: METTL14 promotes chondrocyte ferroptosis in osteoarthritis
            via m6A modification of GPX4.
REFERENCE   3  (bases 1 to 785)
  AUTHORS   Chen,D., Tang,Q., Song,W. and He,Y.
  TITLE     Platelet-derived exosomes alleviate tendon stem/progenitor cell
            senescence and ferroptosis by regulating AMPK/Nrf2/GPX4 signaling
            and improve tendon-bone junction regeneration in rats
  JOURNAL   J Orthop Surg Res 19 (1), 382 (2024)
   PUBMED   38943181
  REMARK    GeneRIF: Platelet-derived exosomes alleviate tendon stem/progenitor
            cell senescence and ferroptosis by regulating AMPK/Nrf2/GPX4
            signaling and improve tendon-bone junction regeneration in rats.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 785)
  AUTHORS   Pfeifer,H., Conrad,M., Roethlein,D., Kyriakopoulos,A.,
            Brielmeier,M., Bornkamm,G.W. and Behne,D.
  TITLE     Identification of a specific sperm nuclei selenoenzyme necessary
            for protamine thiol cross-linking during sperm maturation
  JOURNAL   FASEB J 15 (7), 1236-1238 (2001)
   PUBMED   11344099
REFERENCE   5  (bases 1 to 785)
  AUTHORS   Ursini,F., Heim,S., Kiess,M., Maiorino,M., Roveri,A., Wissing,J.
            and Flohe,L.
  TITLE     Dual function of the selenoprotein PHGPx during sperm maturation
  JOURNAL   Science 285 (5432), 1393-1396 (1999)
   PUBMED   10464096
  REMARK    GeneRIF: Rat Gpx4 is a moonlighting protein that functions both as
            a peroxidase, as well as a structural protein.
REFERENCE   6  (bases 1 to 785)
  AUTHORS   Arai,M., Imai,H., Koumura,T., Yoshida,M., Emoto,K., Umeda,M.,
            Chiba,N. and Nakagawa,Y.
  TITLE     Mitochondrial phospholipid hydroperoxide glutathione peroxidase
            plays a major role in preventing oxidative injury to cells
  JOURNAL   J Biol Chem 274 (8), 4924-4933 (1999)
   PUBMED   9988735
REFERENCE   7  (bases 1 to 785)
  AUTHORS   Pushpa-Rekha,T.R., Burdsall,A.L., Oleksa,L.M., Chisolm,G.M. and
            Driscoll,D.M.
  TITLE     Rat phospholipid-hydroperoxide glutathione peroxidase. cDNA cloning
            and identification of multiple transcription and translation start
            sites
  JOURNAL   J Biol Chem 270 (45), 26993-26999 (1995)
   PUBMED   7592947
REFERENCE   8  (bases 1 to 785)
  AUTHORS   Imai,H., Sumi,D., Hanamoto,A., Arai,M. and Sugiyama,A.
  TITLE     Molecular cloning and functional expression of a cDNA for rat
            phospholipid hydroperoxide glutathione peroxidase: 3'-untranslated
            region of the gene is necessary for functional expression
  JOURNAL   J Biochem 118 (5), 1061-1067 (1995)
   PUBMED   8749327
REFERENCE   9  (bases 1 to 785)
  AUTHORS   Roveri,A., Casasco,A., Maiorino,M., Dalan,P., Calligaro,A. and
            Ursini,F.
  TITLE     Phospholipid hydroperoxide glutathione peroxidase of rat testis.
            Gonadotropin dependence and immunocytochemical identification
  JOURNAL   J Biol Chem 267 (9), 6142-6146 (1992)
   PUBMED   1556123
REFERENCE   10 (bases 1 to 785)
  AUTHORS   Thomas,J.P., Geiger,P.G., Maiorino,M., Ursini,F. and Girotti,A.W.
  TITLE     Enzymatic reduction of phospholipid and cholesterol hydroperoxides
            in artificial bilayers and lipoproteins
  JOURNAL   Biochim Biophys Acta 1045 (3), 252-260 (1990)
   PUBMED   2386798
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JAXUCZ010000007.1.
            
            Summary: The protein encoded by this gene belongs to the
            glutathione peroxidase family, members of which catalyze the
            reduction of hydrogen peroxide, organic hydroperoxides and lipid
            hydroperoxides, and thereby protect cells against oxidative damage.
            Several isozymes of this gene family exist in vertebrates, which
            vary in cellular location and substrate specificity. This isozyme
            has a high preference for lipid hydroperoxides and protects cells
            against membrane lipid peroxidation and cell death. It is also
            required for normal sperm development; thus, it has been identified
            as a 'moonlighting' protein because of its ability to serve dual
            functions as a peroxidase, as well as a structural protein in
            mature spermatozoa. Disruption of this gene in mouse spermatocytes
            is associated with male infertility. This isozyme is also a
            selenoprotein, containing the rare amino acid selenocysteine (Sec)
            at its active site. Sec is encoded by the UGA codon, which normally
            signals translation termination. The 3' UTRs of selenoprotein mRNAs
            contain a conserved stem-loop structure, designated the Sec
            insertion sequence (SECIS) element, that is necessary for the
            recognition of UGA as a Sec codon, rather than as a stop signal.
            Transcript variants resulting from alternative splicing or use of
            alternate promoters have been described to encode isoforms with
            different subcellular localization. Pseudogenes of this locus have
            been identified on chromosomes 1, 10 and 14. [provided by RefSeq,
            Jan 2019].
            
            Transcript Variant: This variant (3) uses an alternate promoter,
            which results in translation initiation from an in-frame downstream
            AUG start codon compared to variant 1. The encoded isoform (C, also
            known as the S-form) has a shorter N-terminus compared to isoform A
            and is localized in the cytoplasm (PMIDs:7592947, 9988735).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ221899.1, FQ234630.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 9988735
            multifunctional gene product(s)    :: PMID: 10464096
            protein contains selenocysteine    :: PMID: 8749327
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                JAXUCZ010000007.1  10303480-10303537   c
            59-153              JAXUCZ010000007.1  10302587-10302681   c
            154-298             JAXUCZ010000007.1  10302351-10302495   c
            299-450             JAXUCZ010000007.1  10302061-10302212   c
            451-475             JAXUCZ010000007.1  10301309-10301333   c
            476-535             JAXUCZ010000007.1  10301165-10301224   c
            536-785             JAXUCZ010000007.1  10300833-10301082   c
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..785
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /note="glutathione peroxidase 4"
                     /db_xref="GeneID:29328"
                     /db_xref="RGD:69226"
     exon            1..58
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..568
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /EC_number="1.11.1.9"
                     /EC_number="1.11.1.12"
                     /note="UGA stop codon recoded as selenocysteine; isoform C
                     is encoded by transcript variant 3; phospholipid
                     hydroperoxide glutathione peroxidase, nuclear;
                     phospholipid hydroperoxide glutathione peroxidase,
                     mitochondrial"
                     /codon_start=1
                     /transl_except=(pos:191..193,aa:Sec)
                     /product="phospholipid hydroperoxide glutathione
                     peroxidase isoform C"
                     /protein_id="NP_001354972.1"
                     /db_xref="GeneID:29328"
                     /db_xref="RGD:69226"
                     /translation="MCASRDDWRCARSMHEFAAKDIDGHMVCLDKYRGCVCIVTNVAS
                     QUGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFAAGYNVRFDM
                     YSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPQ
                     VIEKDLPCYL"
     exon            59..153
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     exon            154..298
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     exon            299..450
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     exon            451..475
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     exon            476..535
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     exon            536..785
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /inference="alignment:Splign:2.1.0"
     regulatory      610..707
                     /regulatory_class="recoding_stimulatory_region"
                     /gene="Gpx4"
                     /gene_synonym="gpx-4; Gshpx-4; Phgpx; snGpx"
                     /note="SECIS_element"
                     /function="essential for recoding UGA to specify
                     selenocysteine"
ORIGIN      
        1 attgaagcca gcactgctgt gcggggctct ggctgtgcct ggcctggctg gcaccatgtg
       61 tgcatcccgc gatgattggc gctgtgcgcg ctccatgcac gaattcgcag ccaaggacat
      121 cgatgggcac atggtttgcc tggataagta caggggttgc gtgtgcatcg tcaccaacgt
      181 ggcctcgcaa tgaggcaaaa ccgacgtaaa ctacactcag ctagtcgatc tgcatgcccg
      241 atacgccgag tgtggtttac gaatcctggc cttcccttgc aaccagttcg ggaggcagga
      301 gccaggaagt aatcaagaaa tcaaggagtt tgcagccggc tacaatgtca ggtttgacat
      361 gtacagcaag atctgtgtaa atggggacga tgcccaccca ctgtggaaat ggatgaaagt
      421 ccagcccaag ggcaggggca tgctgggaaa tgccatcaaa tggaacttta ccaagtttct
      481 cattgataag aacggctgcg tggtgaagcg ctatggtccc atggaggagc cccaggtgat
      541 agagaaggac ctgccgtgct atctctagcc ctacaagtgt gtgcccctgc accgagcccc
      601 cctgccctgt gacccctgga gccttccacc ccggcactca tgacggtctg cctgaaaacc
      661 agcccgctgg tggggcagtc ccgaggacct ggcgtgcatc cccgccggag gaaggtccag
      721 aggcctgtgg ccccgggctc gagcttcacc ttggctgcct tgtgggaata aaatgtagaa
      781 atgtg
//
