LOCUS       NM_022865               3222 bp    mRNA    linear   ROD 24-JUN-2024
DEFINITION  Rattus norvegicus gephyrin (Gphn), mRNA.
ACCESSION   NM_022865 XM_346771
VERSION     NM_022865.4
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Al Awabdh,S., Donneger,F., Goutierre,M., Seveno,M., Vigy,O.,
            Weinzettl,P., Russeau,M., Moutkine,I., Levi,S., Marin,P. and
            Poncer,J.C.
  TITLE     Gephyrin Interacts with the K-Cl Cotransporter KCC2 to Regulate Its
            Surface Expression and Function in Cortical Neurons
  JOURNAL   J Neurosci 42 (2), 166-182 (2022)
   PUBMED   34810232
  REMARK    GeneRIF: Gephyrin Interacts with the K-Cl Cotransporter KCC2 to
            Regulate Its Surface Expression and Function in Cortical Neurons.
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Shen,Z.C., Wu,P.F., Wang,F., Xia,Z.X., Deng,Q., Nie,T.L.,
            Zhang,S.Q., Zheng,H.L., Liu,W.H., Lu,J.J., Gao,S.Q., Yao,X.P.,
            Long,L.H., Hu,Z.L. and Chen,J.G.
  TITLE     Gephyrin Palmitoylation in Basolateral Amygdala Mediates the
            Anxiolytic Action of Benzodiazepine
  JOURNAL   Biol Psychiatry 85 (3), 202-213 (2019)
   PUBMED   30454851
  REMARK    GeneRIF: results support the view that the strength of inhibitory
            synapse was controlled by gephyrin palmitoylation.
REFERENCE   3  (bases 1 to 3222)
  AUTHORS   Grunewald,N., Jan,A., Salvatico,C., Kress,V., Renner,M.,
            Triller,A., Specht,C.G. and Schwarz,G.
  TITLE     Sequences Flanking the Gephyrin-Binding Site of GlyRbeta Tune
            Receptor Stabilization at Synapses
  JOURNAL   eNeuro 5 (1) (2018)
   PUBMED   29464196
  REMARK    GeneRIF: Study shows that the glycine receptors beta-loop sequences
            flanking the core-binding site differentially regulate the affinity
            of the receptor for gephyrin and its trapping at synapses.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3222)
  AUTHORS   Battaglia,S., Renner,M., Russeau,M., Come,E., Tyagarajan,S.K. and
            Levi,S.
  TITLE     Activity-Dependent Inhibitory Synapse Scaling Is Determined by
            Gephyrin Phosphorylation and Subsequent Regulation of GABAA
            Receptor Diffusion
  JOURNAL   eNeuro 5 (1) (2018)
   PUBMED   29379879
  REMARK    GeneRIF: Study shows that activity-dependent inhibitory synapse
            scaling is determined by gephyrin phosphorylation and subsequent
            regulation of GABAA receptor diffusion.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3222)
  AUTHORS   Zhou,W., Zhou,L.Q., Shi,H., Leng,Y.M., Liu,B., Zhang,S.L. and
            Kong,W.J.
  TITLE     Expression of glycine receptors and gephyrin in rat medial
            vestibular nuclei and flocculi following unilateral labyrinthectomy
  JOURNAL   Int J Mol Med 38 (5), 1481-1489 (2016)
   PUBMED   28026001
  REMARK    GeneRIF: the role of gephyrin and glycine receptor (GlyR) in
            vestibular compensation, was investigated.
REFERENCE   6  (bases 1 to 3222)
  AUTHORS   Feng,G., Tintrup,H., Kirsch,J., Nichol,M.C., Kuhse,J., Betz,H. and
            Sanes,J.R.
  TITLE     Dual requirement for gephyrin in glycine receptor clustering and
            molybdoenzyme activity
  JOURNAL   Science 282 (5392), 1321-1324 (1998)
   PUBMED   9812897
REFERENCE   7  (bases 1 to 3222)
  AUTHORS   Colin,I., Rostaing,P., Augustin,A. and Triller,A.
  TITLE     Localization of components of glycinergic synapses during rat
            spinal cord development
  JOURNAL   J Comp Neurol 398 (3), 359-372 (1998)
   PUBMED   9714149
REFERENCE   8  (bases 1 to 3222)
  AUTHORS   Sassoe-Pognetto,M. and Wassle,H.
  TITLE     Synaptogenesis in the rat retina: subcellular localization of
            glycine receptors, GABA(A) receptors, and the anchoring protein
            gephyrin
  JOURNAL   J Comp Neurol 381 (2), 158-174 (1997)
   PUBMED   9130666
REFERENCE   9  (bases 1 to 3222)
  AUTHORS   Kirsch,J., Wolters,I., Triller,A. and Betz,H.
  TITLE     Gephyrin antisense oligonucleotides prevent glycine receptor
            clustering in spinal neurons
  JOURNAL   Nature 366 (6457), 745-748 (1993)
   PUBMED   8264797
REFERENCE   10 (bases 1 to 3222)
  AUTHORS   Prior,P., Schmitt,B., Grenningloh,G., Pribilla,I., Multhaup,G.,
            Beyreuther,K., Maulet,Y., Werner,P., Langosch,D., Kirsch,J. et al.
  TITLE     Primary structure and alternative splice variants of gephyrin, a
            putative glycine receptor-tubulin linker protein
  JOURNAL   Neuron 8 (6), 1161-1170 (1992)
   PUBMED   1319186
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000006.1.
            
            [WARNING] On Oct 22, 2024 this sequence was replaced by
            NM_022865.5.
            
            On Nov 27, 2020 this sequence version replaced NM_022865.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X66366.2, SRR26360176.911335.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-209               JAXUCZ010000006.1  102687653-102687861
            210-288             JAXUCZ010000006.1  102802585-102802663
            289-346             JAXUCZ010000006.1  102834074-102834131
            347-439             JAXUCZ010000006.1  102882304-102882396
            440-534             JAXUCZ010000006.1  102934033-102934127
            535-601             JAXUCZ010000006.1  102947504-102947570
            602-874             JAXUCZ010000006.1  102956785-102957057
            875-1009            JAXUCZ010000006.1  102983946-102984080
            1010-1052           JAXUCZ010000006.1  103039004-103039046
            1053-1190           JAXUCZ010000006.1  103076589-103076726
            1191-1283           JAXUCZ010000006.1  103103312-103103404
            1284-1339           JAXUCZ010000006.1  103126717-103126772
            1340-1459           JAXUCZ010000006.1  103140097-103140216
            1460-1518           JAXUCZ010000006.1  103142134-103142192
            1519-1672           JAXUCZ010000006.1  103143241-103143394
            1673-1794           JAXUCZ010000006.1  103151483-103151604
            1795-1882           JAXUCZ010000006.1  103188898-103188985
            1883-1956           JAXUCZ010000006.1  103195792-103195865
            1957-2021           JAXUCZ010000006.1  103200835-103200899
            2022-2125           JAXUCZ010000006.1  103212171-103212274
            2126-2222           JAXUCZ010000006.1  103214422-103214518
            2223-3222           JAXUCZ010000006.1  103215680-103216679
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q24"
     gene            1..3222
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /note="gephyrin"
                     /db_xref="GeneID:64845"
                     /db_xref="RGD:69194"
     exon            1..209
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     CDS             146..2356
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /EC_number="2.10.1.1"
                     /EC_number="2.7.7.75"
                     /note="putative glycine receptor-tubulin linker protein"
                     /codon_start=1
                     /product="gephyrin"
                     /protein_id="NP_074056.2"
                     /db_xref="GeneID:64845"
                     /db_xref="RGD:69194"
                     /translation="MATEGMILTNHDHQIRVGVLTVSDSCFRNLAEDRSGINLKDLVQ
                     DPSLLGGTISAYKIVPDEIEEIKETLIDWCDEKELNLILTTGGTGFAPRDVTPEATKE
                     VIEREAPGMALAMLMGSLNVTPLGMLSRPVCGIRGKTLIINLPGSKKGSQECFQFILP
                     ALPHAIDLLRDAIVKVKEVHDELEDLPSPPPPLSPPPTTSPHKQTEDKGVQCEEEEEE
                     KKDSGVASTEDSSSSHITAAALAAKIPDSIISRGVQVLPRDTASLSTTPSESPRAQAT
                     SRLSTASCPTPKVQSRCSSKENILRASHSAVDITKVARRHRMSPFPLTSMDKAFITVL
                     EMTPVLGTEIINYRDGMGRVLAQDVYAKDNLPPFPASVKDGYAVRAADGPGDRFIIGE
                     SQAGEQPTQTVMPGQVMRVTTGAPIPCGADAVVQVEDTELIRESDDGTEELEVRILVQ
                     ARPGQDIRPIGHDIKRGECVLAKGTHMGPSEIGLLATVGVTEVEVNKFPVVAVMSTGN
                     ELLNPEDDLLPGKIRDSNRSTLLATIQEHGYPTINLGIVGDNPDDLLNALNEGISRAD
                     VIITSGGVSMGEKDYLKQVLDIDLHAQIHFGRVFMKPGLPTTFATLDIDGVRKIIFAL
                     PGNPVSAVVTCNLFVVPALRKMQGILDPRPTIIKARLSCDVKLDPRPEYHRCILTWHH
                     QEPLPWAQSTGNQMSSRLMSMRSANGLLMLPPKTEQYVELHKGEVVDVMVIGRL"
     exon            210..288
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            289..346
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            347..439
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            440..534
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            535..601
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            602..874
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1009
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1052
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1190
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1283
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1339
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1459
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1518
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1672
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1794
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            1795..1882
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            1883..1956
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            1957..2021
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2125
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2222
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
     exon            2223..3222
                     /gene="Gphn"
                     /gene_synonym="Geph"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgctcggccc cctccttccc gctcgcctcg cgctccccgg gcgccctcgt tctcgcgctc
       61 tccaaggcaa gcgcgctccc ggccggcgcg ctccgggctc cggcttctcc cggctcctgt
      121 cagtgcggtg attgcgctgg gaaacatggc gaccgaggga atgatcctca caaaccacga
      181 ccatcaaatc cgtgtcggag tcctcacagt gagtgatagc tgcttcagga atcttgcaga
      241 agaccgcagt gggataaatc taaaagatct cgtccaggat ccttctttgt tgggtgggac
      301 tatatcagca tacaagatag taccagatga aatagaagaa atcaaggaaa cactcataga
      361 ctggtgtgat gagaaggaac ttaatttgat attaacaact ggcggaacag ggtttgcacc
      421 acgagatgtc actccagagg ccacaaaaga agtaatagaa cgggaagcgc cagggatggc
      481 cctggcaatg ctgatgggat cacttaatgt tacacctctg ggcatgctct ctagaccagt
      541 gtgtggaata agagggaaaa ctctgataat taacctgcct ggtagcaaga aaggatctca
      601 ggaatgcttt cagttcatac tgccagctct acctcatgcc attgaccttt tacgtgatgc
      661 cattgtaaaa gtaaaggagg tgcatgatga acttgaagat ttaccttccc caccacctcc
      721 tctttctcca cctcctacaa ctagcccaca taagcagaca gaagacaaag gagttcagtg
      781 tgaagaggag gaagaagaaa agaaagacag tggtgtagct tcgacagaag atagttcctc
      841 atcacatatt actgcagcag ctcttgctgc aaagattcca gactccatca tttctcgtgg
      901 cgttcaggtg ctcccacgag acacagcctc ccttagcact actccttcag agtcgccccg
      961 tgcccaggct acatctcgcc tctctacagc ttcttgtcca acaccaaaag tccagtccag
     1021 gtgcagcagc aaggagaaca ttctaagagc cagtcacagt gctgtagata tcaccaaggt
     1081 ggctagaaga catcgcatgt ctccttttcc tctgacgtct atggacaaag ccttcattac
     1141 agttctggag atgactccgg tgcttggtac agaaatcatc aattaccgag atggaatggg
     1201 gcgagtcctt gctcaagatg tgtatgcaaa agacaaccta cccccattcc cagcatcagt
     1261 aaaagatggc tatgctgttc gagctgctga tggtccagga gatcgtttca tcattgggga
     1321 atcccaagct ggtgaacagc caactcagac agtaatgccc ggacaagtga tgcgggttac
     1381 aacaggtgct ccaatcccct gcggtgctga tgcagtagtg caagttgaag atactgaact
     1441 catcagggaa tcagatgatg gtactgaaga acttgaagtg cgaattctgg tgcaagctcg
     1501 gccaggccaa gacatcagac ccatcggcca cgacattaaa agaggggagt gtgttttggc
     1561 aaaaggaacc catatgggcc cttcagaaat tggtctcctt gcaactgtag gtgtcacaga
     1621 agttgaagtt aataagtttc cagtggttgc cgttatgtca acagggaatg agctactaaa
     1681 tcctgaagat gacctcttac caggaaagat tcgggacagc aatcgatcaa cacttctagc
     1741 aacaattcag gaacatggtt acccaacaat caacttggga attgtaggag acaacccaga
     1801 tgacttactc aatgccttga atgagggtat cagccgtgct gatgtcatca tcacgtcagg
     1861 aggtgtatcc atgggggaaa aggactatct caagcaggtg ctggacatcg atcttcatgc
     1921 tcagatccat tttggcaggg tttttatgaa gccaggcttg ccaacaacat ttgcaacttt
     1981 ggatattgat ggtgtaagaa aaattatttt tgcactacca gggaatcctg tatcagctgt
     2041 ggtcacctgc aacctctttg ttgtacccgc actgagaaaa atgcagggta tcttggatcc
     2101 tcggccaacc atcatcaaag ctaggttatc atgtgacgta aagctggacc ctcgcccaga
     2161 ataccaccgg tgtatactga cttggcatca ccaagaacca ctgccctggg cccagagtac
     2221 aggtaatcag atgagcagcc gtctgatgag catgcgcagt gccaatggat tgctgatgct
     2281 acctccaaag acagagcagt atgtggaact tcacaaaggc gaggtggtgg acgtcatggt
     2341 catcggacgg ctatgatggt gaccaacagg agaaagtttc gatgcatgtc cacatatcat
     2401 tgactgtatc ctgtcatatg caacggcaca gctagttttt ctgatctgga taaaagttga
     2461 tctgtatagt caacatcttg aactatattt caaatgaatt taaatacctt ttaaagaaaa
     2521 aacacaccta agaataaatc tcaacagaca actctattct ggttatatca aagcaaaatt
     2581 ttcctttctt gcaaattgct ttgtgtgttc aatgctaggt ctgatagcga tagctttagt
     2641 agacagcggt aggtgcctgc agaacttgtg tttttctcat ctttaaatac aactacttat
     2701 gctcttaatc aaggctgtct gcttctttat actagtgtag gcaacacttg gatttccctt
     2761 cttagtatgc ttcataactg ctttacagag agcttttgct tgttctttct catgtatctc
     2821 gtgtttatgt gcaccatgcc aaaagacgag tgactgcgcg gaggccccgc tctgcctcaa
     2881 gagccttccc tgctgagcac ccagggaagc tctcaccctc gcagcctctg gcacacacag
     2941 tactagcagg cagtaactgg atacctttta tttgaacaaa caaacggggt ggggggaaat
     3001 gcttccttaa gtgctgacag cctttttaac caacacattt aaaattgtac agaacaaaaa
     3061 taaaatcaaa gactgatctt gtacagatat tagtgttacc agcattcatg tggaatcaag
     3121 agcaaaaata aaataatgtt aaacaactct gtaccataac attttctgta atgataatga
     3181 aacttaatga ataaaaaaaa atccttgatc attatttaaa aa
//
