LOCUS       NM_001014108            2669 bp    mRNA    linear   ROD 23-MAR-2023
DEFINITION  Rattus norvegicus glypican 4 (Gpc4), mRNA.
ACCESSION   NM_001014108 XM_228677
VERSION     NM_001014108.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   White JJ, Mazzeu JF, Coban-Akdemir Z, Bayram Y, Bahrambeigi V,
            Hoischen A, van Bon BWM, Gezdirici A, Gulec EY, Ramond F, Touraine
            R, Thevenon J, Shinawi M, Beaver E, Heeley J, Hoover-Fong J, Durmaz
            CD, Karabulut HG, Marzioglu-Ozdemir E, Cayir A, Duz MB, Seven M,
            Price S, Ferreira BM, Vianna-Morgante AM, Ellard S, Parrish A,
            Stals K, Flores-Daboub J, Jhangiani SN, Gibbs RA, Brunner HG,
            Sutton VR, Lupski JR and Carvalho CMB.
  CONSRTM   Baylor-Hopkins Center for Mendelian Genomics
  TITLE     WNT Signaling Perturbations Underlie the Genetic Heterogeneity of
            Robinow Syndrome
  JOURNAL   Am J Hum Genet 102 (1), 27-43 (2018)
   PUBMED   29276006
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Xiong Y, Zhang Y, Zheng F, Yang Y, Xu X, Wang W, Zhu B and Wang X.
  TITLE     Expression of Glypican-4 in the brains of epileptic patients and
            epileptic animals and its effects on epileptic seizures
  JOURNAL   Biochem Biophys Res Commun 478 (1), 241-246 (2016)
   PUBMED   27425250
  REMARK    GeneRIF: According to behavioral studies, downregulation of Gpc4 by
            Gpc4 siRNA decreased spontaneous seizure frequency, while
            upregulation of Gpc4 by recombinant Gpc4 overexpression led to a
            converse result. These findings support the hypothesis that
            increased expression of Gpc4 in the brain is associated with
            epileptic seizures.
REFERENCE   3  (bases 1 to 2669)
  AUTHORS   Ko JS, Pramanik G, Um JW, Shim JS, Lee D, Kim KH, Chung GY,
            Condomitti G, Kim HM, Kim H, de Wit J, Park KS, Tabuchi K and Ko J.
  TITLE     PTPsigma functions as a presynaptic receptor for the
            glypican-4/LRRTM4 complex and is essential for excitatory synaptic
            transmission
  JOURNAL   Proc Natl Acad Sci U S A 112 (6), 1874-1879 (2015)
   PUBMED   25624497
  REMARK    GeneRIF: GPC-4 showed strong (nanomolar) affinity and heparan
            sulfate-dependent interaction with the Ig domains of PTPsigma in
            rat brain synapses.
REFERENCE   4  (bases 1 to 2669)
  AUTHORS   Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X,
            Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K,
            Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A and Mayr
            M.
  TITLE     Extracellular matrix secretion by cardiac fibroblasts: role of
            microRNA-29b and microRNA-30c
  JOURNAL   Circ Res 113 (10), 1138-1147 (2013)
   PUBMED   24006456
REFERENCE   5  (bases 1 to 2669)
  AUTHORS   de Wit J, O'Sullivan ML, Savas JN, Condomitti G, Caccese MC,
            Vennekens KM, Yates JR 3rd and Ghosh A.
  TITLE     Unbiased discovery of glypican as a receptor for LRRTM4 in
            regulating excitatory synapse development
  JOURNAL   Neuron 79 (4), 696-711 (2013)
   PUBMED   23911103
REFERENCE   6  (bases 1 to 2669)
  AUTHORS   Williams EH, Pappano WN, Saunders AM, Kim MS, Leahy DJ and Beachy
            PA.
  TITLE     Dally-like core protein and its mammalian homologues mediate
            stimulatory and inhibitory effects on Hedgehog signal response
  JOURNAL   Proc Natl Acad Sci U S A 107 (13), 5869-5874 (2010)
   PUBMED   20231458
REFERENCE   7  (bases 1 to 2669)
  AUTHORS   Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE and
            Yates JR.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   8  (bases 1 to 2669)
  AUTHORS   Trinidad JC, Specht CG, Thalhammer A, Schoepfer R and Burlingame
            AL.
  TITLE     Comprehensive identification of phosphorylation sites in
            postsynaptic density preparations
  JOURNAL   Mol Cell Proteomics 5 (5), 914-922 (2006)
   PUBMED   16452087
REFERENCE   9  (bases 1 to 2669)
  AUTHORS   Hagihara K, Watanabe K, Chun J and Yamaguchi Y.
  TITLE     Glypican-4 is an FGF2-binding heparan sulfate proteoglycan
            expressed in neural precursor cells
  JOURNAL   Dev Dyn 219 (3), 353-367 (2000)
   PUBMED   11066092
REFERENCE   10 (bases 1 to 2669)
  AUTHORS   Siebertz B, Stocker G, Drzeniek Z, Handt S, Just U and Haubeck HD.
  TITLE     Expression of glypican-4 in haematopoietic-progenitor and
            bone-marrow-stromal cells
  JOURNAL   Biochem J 344 Pt 3 (Pt 3), 937-943 (1999)
   PUBMED   10585884
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC081962.1.
            
            On Apr 20, 2005 this sequence version replaced XM_228677.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081962.1, SRR8487227.176117.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMN12840111
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="X"
                     /map="Xq36"
     gene            1..2669
                     /gene="Gpc4"
                     /note="glypican 4"
                     /db_xref="GeneID:317322"
                     /db_xref="RGD:1359399"
     exon            1..675
                     /gene="Gpc4"
                     /inference="alignment:Splign:2.1.0"
     CDS             516..2189
                     /gene="Gpc4"
                     /codon_start=1
                     /product="glypican-4 precursor"
                     /protein_id="NP_001014130.1"
                     /db_xref="GeneID:317322"
                     /db_xref="RGD:1359399"
                     /translation="MARLGLLALLCTLAALSASLLAAEPKSKSCSEVRRLYVSKGFNK
                     NDAPLYEINGDHLKICSQDYTCCSQEMEEKYSLQSKDDFQTVVSEQCNHLQAIFASRY
                     KKFDEFFKELLENAEKSLNDMFVKTYGHLYMQNSELFKDLFVELKRYYVAGNVNLEEM
                     LNDFWARLLERMFRLVNSQYHFTDEYLECVSKYTEQLKPFGDVPRKLKLQVTRAFVAA
                     RTFAQGLAVARDVVNRVSVVNPTAQCTQALLKMIYCSHCRGLVTVKPCYNYCSNIMRG
                     CLANQGDLDLEWNNFIDAMLMVAERLEGPFNIESVMDPIDVKISDAIMNMQDNSVQVS
                     QKVFQGCGPPKPLPAGRISRSISESAFSARFRPYHPEQRPTTAAGTSLDRLVTDVKEK
                     LKQAKKFWSSLPSTVCNDERMAAGNENEDDCWNGKSKSRYLFAVTGNGLANQGNNPEV
                     QVDTSKPDILILRQIMALRVMTSKMKNAYNGNDVDFFDISDESSGEGSGSGCEYQQCP
                     SEFEYNATDHSGKSANEKADSAGGAPAEAKPYLLAALCILFLAVQGEWR"
     exon            676..834
                     /gene="Gpc4"
                     /inference="alignment:Splign:2.1.0"
     exon            835..1226
                     /gene="Gpc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1392
                     /gene="Gpc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1523
                     /gene="Gpc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1670
                     /gene="Gpc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1807
                     /gene="Gpc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1808..1983
                     /gene="Gpc4"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2642
                     /gene="Gpc4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctgagaagg gcacagccgt gcggccgagg gcagtgagct gagccgagct gggtgagcgg
       61 agcagcgccg ggtagctgga gccccgcgcc agcgatcacc cgccgacgcc gcccacactc
      121 tgctctcctg cgacgcctgc caccttccct tccttccctg agcccctcca cccctggcta
      181 gctggcccgg gttctctagc cttcagagtc ccgaggtcac cgatcctttt cctagtcccg
      241 cgcccattcc acgccttttc ccttgtaccc caccccggct gggcgcctgg cacgggggac
      301 cgttgcctga cgcgaggccc agctccactt ttcgcctggg tctcctcctc ctcctgtcct
      361 cctcctcctc cttcactacc aactccaact ccccggttca cgctccgctt gtgagtgttg
      421 actccgcgcc tctttgcccg ctcccgcagc gctgctttgc atccagtctc attctgtccc
      481 ctaaaggtgg gagcgcgtcc gtcagggtcc gcaccatggc acggttaggc ttgctcgcgc
      541 ttctctgcac tctggcggcg ctcagcgcct cgctgctggc tgcggagccc aagtcgaaaa
      601 gttgttcgga agtgcgacgt ctctacgtgt ccaaaggctt caacaagaac gatgcccccc
      661 tctatgagat caatggtgac catttgaaga tctgttccca ggattataca tgctgttctc
      721 aagagatgga ggagaagtac agtctgcaaa gtaaagatga tttccaaact gtggtcagcg
      781 agcagtgcaa ccatttgcaa gccatctttg catcccgtta caagaagttt gatgaatttt
      841 tcaaagaact tcttgaaaat gcagagaaat ccctgaatga tatgttcgtg aagacatacg
      901 gccacttata catgcaaaat tcagagctat ttaaagatct gttcgtagag ttgaagcgct
      961 actatgtggc gggaaacgtg aacctggaag aaatgttaaa tgacttctgg gctcgccttc
     1021 tggagcgcat gttccgcctg gtgaactccc agtaccattt cacagatgag tacctggaat
     1081 gtgtgagcaa atacactgag cagctgaagc cttttggaga cgtccctcgg aaactgaagc
     1141 tccaggttac ccgtgcattc gttgcagcca ggaccttcgc tcaaggctta gcagttgcaa
     1201 gggatgtcgt gaacagagtc tctgtggtga atcccacagc tcagtgcacc caagcactgc
     1261 tgaagatgat ctactgctct cactgccggg gcctggtgac tgtgaagccc tgttacaact
     1321 attgctcaaa catcatgaga ggctgtttgg ccaaccaagg agaccttgat ttagagtgga
     1381 acaatttcat agatgccatg ctgatggtgg cagagaggct ggagggtcct ttcaacattg
     1441 agtccgttat ggatcccatc gatgtgaaga tttctgatgc tatcatgaac atgcaggata
     1501 atagtgttca ggtgtctcag aaggttttcc aaggctgtgg acctccaaag cctctcccgg
     1561 ctggacgaat ttctcgttcc atctctgaaa gtgccttcag tgctcgattc agaccttatc
     1621 atccagagca acgcccaact acagcagctg gcactagttt ggaccgactg gttactgatg
     1681 tcaaagagaa actgaagcaa gctaagaagt tctggtcctc tctcccaagc actgtctgca
     1741 atgatgagag gatggcagca ggaaatgaga atgaggatga ctgttggaac gggaagagca
     1801 aaagcaggta cctgtttgca gtgacaggaa acggattagc caaccagggc aacaacccag
     1861 aagtgcaggt tgacaccagc aagccggaca ttctgatcct tcgtcaaatc atggcccttc
     1921 gggttatgac cagtaaaatg aagaatgctt acaacggaaa tgacgtggac ttctttgata
     1981 tcagcgatga gagtagtgga gagggaagcg gaagcggatg tgaatatcag cagtgccctt
     2041 cagagtttga gtacaacgcc actgaccatt ctgggaagag tgccaacgag aaagctgaca
     2101 gtgccggtgg tgcccctgca gaggcaaagc cctatctcct cgctgccctc tgcatcctgt
     2161 tcctcgctgt gcagggagag tggagataat tgtcaaactc tgagtaaaag tgttcatcat
     2221 cagaaagttc aaaggcacca gttctcactt tttttttttt aatcatccta gtgactttgc
     2281 ttttaaatga atggacaaca atgtacagtt tttactgtgt ggccactggt ttaagcaatg
     2341 ttgacttgtt gttgactttg tttttctcat tcagtttgag gggacagggg cctcctgcct
     2401 tcaagtgtgt ccctccgcac tcccctcctt tgagactcgt gttatgcgcg gctagcggtg
     2461 tagttacaga actgtagtta gttgtgtatt ggtgatcttg ttttctttgt tggtttttct
     2521 catttgtggg gttttccttt tccaattatg gtctcacctt gtttctcaca agaaaaccag
     2581 ggtcgtttct tggcatgtaa catgtacgta tttctgaaat attaaatagc tgtacagaag
     2641 caaaaaaaaa aaaaaaaaaa aaaaaaaaa
//
