LOCUS       NM_134418               1919 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus glycoprotein 2 (Gp2), mRNA.
ACCESSION   NM_134418 XM_346554
VERSION     NM_134418.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Hase,K., Kawano,K., Nochi,T., Pontes,G.S., Fukuda,S., Ebisawa,M.,
            Kadokura,K., Tobe,T., Fujimura,Y., Kawano,S., Yabashi,A.,
            Waguri,S., Nakato,G., Kimura,S., Murakami,T., Iimura,M., Hamura,K.,
            Fukuoka,S., Lowe,A.W., Itoh,K., Kiyono,H. and Ohno,H.
  TITLE     Uptake through glycoprotein 2 of FimH(+) bacteria by M cells
            initiates mucosal immune response
  JOURNAL   Nature 462 (7270), 226-230 (2009)
   PUBMED   19907495
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Yu,S., Hao,Y. and Lowe,A.W.
  TITLE     Effects of GP2 expression on secretion and endocytosis in
            pancreatic AR4-2J cells
  JOURNAL   Biochem Biophys Res Commun 322 (1), 320-325 (2004)
   PUBMED   15313209
  REMARK    GeneRIF: Thus, GP2 expression in AR4-2J cells does not affect
            amylase packaging in secretory granules or stimulated secretion.
            GP2 expression also does not influence membrane recycling in
            response to stimulated stimulation in AR4-2J cells.
REFERENCE   3  (bases 1 to 1919)
  AUTHORS   Fritz,B.A., Poppel,C.S., Fei,M.W. and Lowe,A.W.
  TITLE     Processing of the major pancreatic zymogen granule membrane
            protein, GP2
  JOURNAL   Pancreas 24 (4), 336-343 (2002)
   PUBMED   11961485
  REMARK    GeneRIF: GP2 undergoes sequential intracellular cleavage during
            secretion.
REFERENCE   4  (bases 1 to 1919)
  AUTHORS   Withiam-Leitch,M., Aletta,J.M., Koshlukova,S.E., Rupp,G.,
            Beaudoin,A.R. and Rubin,R.P.
  TITLE     Glycoprotein 2 of zymogen granule membranes shares immunological
            cross-reactivity and sequence similarity with phospholipase A2
  JOURNAL   Biochem Biophys Res Commun 194 (3), 1167-1174 (1993)
   PUBMED   8352773
REFERENCE   5  (bases 1 to 1919)
  AUTHORS   Hoops,T.C. and Rindler,M.J.
  TITLE     Isolation of the cDNA encoding glycoprotein-2 (GP-2), the major
            zymogen granule membrane protein. Homology to
            uromodulin/Tamm-Horsfall protein
  JOURNAL   J Biol Chem 266 (7), 4257-4263 (1991)
   PUBMED   1999417
REFERENCE   6  (bases 1 to 1919)
  AUTHORS   Fukuoka,S. and Scheele,G.
  TITLE     Nucleotide sequence encoding the major glycoprotein (GP2) of rat
            pancreatic secretory (zymogen) granule membranes
  JOURNAL   Nucleic Acids Res 18 (19), 5900 (1990)
   PUBMED   2216794
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Sep 15, 2012 this sequence version replaced NM_134418.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M58716.1, SRR26643287.2037.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                JAXUCZ010000001.1  183243913-183243954 c
            43-178              JAXUCZ010000001.1  183242708-183242843 c
            179-622             JAXUCZ010000001.1  183239898-183240341 c
            623-733             JAXUCZ010000001.1  183238494-183238604 c
            734-945             JAXUCZ010000001.1  183237800-183238011 c
            946-1094            JAXUCZ010000001.1  183237172-183237320 c
            1095-1340           JAXUCZ010000001.1  183235773-183236018 c
            1341-1503           JAXUCZ010000001.1  183234652-183234814 c
            1504-1588           JAXUCZ010000001.1  183232945-183233029 c
            1589-1633           JAXUCZ010000001.1  183231284-183231328 c
            1634-1919           JAXUCZ010000001.1  183228395-183228680 c
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q35"
     gene            1..1919
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /note="glycoprotein 2"
                     /db_xref="GeneID:171459"
                     /db_xref="RGD:621695"
     exon            1..42
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /inference="alignment:Splign:2.1.0"
     exon            43..178
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..1692
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /note="pancreatic secretory granule membrane major
                     glycoprotein GP2; glycoprotein 80; pancreatic zymogen
                     granule membrane protein GP-2; secretory (zymogen) granule
                     membrane glycoprotein GP2; glycoprotein 2 (zymogen granule
                     membrane)"
                     /codon_start=1
                     /product="pancreatic secretory granule membrane major
                     glycoprotein GP2 precursor"
                     /protein_id="NP_599245.2"
                     /db_xref="GeneID:171459"
                     /db_xref="RGD:621695"
                     /translation="MKRMVACDLLWLAAASCLLTLVFPSTTHQGYGNPRNTSNVDLDC
                     GAPGSSSAGICFDPCQNHTVLNDPSRSTENTVSSEECDSHLRGWYRFVGDGGVKMPET
                     CVNVYRCHTYAPMWLSESHPILGDGIVNRTACANWNENCCFWSSEVQVKACLGESGEY
                     HVYKLQGTPECSLRYCTDPSTAPKKCEIACRPEEECVFQNNSWTCVCRQDLNVSDPQS
                     LQPLLDCGANEIKVKLDKCLLGGLGFKEDIITYLNDRNCRGTMKDEPNNWVSTTSPVV
                     ANDCGNILENNGTHAIYRNTLSLATDFIIRDFLVNVNFQCAYPLDMNVSLQTALQPIV
                     SSLNVDVGGAGEFTVTMALFQDQSYTHPYEGSKVLLPVENILYVGVLLNRGDTSRFKL
                     LLTNCYATPSGDRNDIVKYFIIRNRCPNQRDSTINVEENGVSSESRFSVQMFMFAGNY
                     DLVFLHCEVYLCDSTTEQCQPSCSTSRLRSSEPAIDLTRVLDIGPITKKSVQNPDTSN
                     GTPRSTGFLLAWPTFFLPVFLAWLF"
     exon            179..622
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /inference="alignment:Splign:2.1.0"
     exon            623..733
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /inference="alignment:Splign:2.1.0"
     exon            734..945
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1094
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1340
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1503
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /inference="alignment:Splign:2.1.0"
     exon            1504..1588
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /inference="alignment:Splign:2.1.0"
     exon            1589..1633
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1919
                     /gene="Gp2"
                     /gene_synonym="gp80"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cacactgggc agaggaagga ggatactctg gcttctttta tgatttatgg aggatctgca
       61 ggctgcagaa taaccagcaa gcctcacttt atgaaaagga tggtggcttg tgacctgctg
      121 tggctggctg cagcctcctg ccttctgaca ctggtatttc catctacaac acatcaaggt
      181 tatggaaacc ccagaaacac ctccaatgtg gacttggact gtggagcccc cggctcatca
      241 agtgctggca tctgctttga cccctgccag aatcacaccg tcctgaatga tccctccaga
      301 agcacagaga acacggtgtc atcggaggag tgtgatagtc acctgagggg ctggtaccgc
      361 tttgtgggtg atgggggagt gaagatgcca gagacctgtg tgaatgtgta ccggtgccac
      421 acctatgctc ccatgtggct gagtgaatca caccccatcc ttggagatgg cattgtcaac
      481 cgcacagcct gtgccaactg gaatgaaaat tgttgtttct ggagttctga ggtccaggtg
      541 aaggcctgtt tgggagaatc aggagaatat cacgtgtaca agttacaggg cacccctgaa
      601 tgcagtctga gatactgtac agacccctcc accgcaccga aaaagtgtga gattgcttgt
      661 cgtcctgagg aggaatgtgt attccaaaat aacagctgga cctgtgtctg taggcaagac
      721 ctcaatgttt ctgaccctca gagtttgcag cctcttctgg actgtggggc taatgagatc
      781 aaggtgaagc tggacaagtg tttgctggga ggtctgggtt tcaaggagga catcatcacc
      841 tacctgaatg accggaactg cagaggtacc atgaaagatg agcccaacaa ctgggtgtcc
      901 acgaccagcc ctgttgtggc taatgactgt gggaacatcc tggagaacaa tgggacccat
      961 gccatctata gaaacaccct ctccttggcc actgatttca ttatcaggga cttccttgtc
     1021 aacgtcaact tccagtgtgc ctaccctctg gacatgaatg tcagtctcca aactgcactc
     1081 cagcccatcg taagttccct gaatgttgat gtgggtggag caggagagtt cactgttacg
     1141 atggcactct tccaagacca aagctacaca cacccttatg aaggaagcaa agtgttgctt
     1201 ccagtggaga atatactcta cgtgggcgtc ctcttgaata ggggtgatac ctccaggttc
     1261 aaactgttgc tgaccaactg ctatgccacc ccttcaggag acaggaatga cattgtgaag
     1321 tacttcatca tcaggaacag atgcccaaac caacgtgatt ccaccatcaa tgtggaggag
     1381 aatggggtgt cctcggaaag ccgattttca gtgcagatgt tcatgtttgc tggaaattat
     1441 gacctagttt tcctgcattg tgaggtttac ctgtgtgact ccactactga acagtgtcaa
     1501 ccatcttgct ctacaagtcg acttcgcagt agtgaaccag ccatcgactt aactcgggtt
     1561 ctggatatag gacccatcac taagaaaagt gttcagaatc ctgatacctc caacgggact
     1621 ccccgaagta cagggttcct gttggcctgg cccacgttct tcctgcctgt cttcctggcc
     1681 tggctgttct gagagtccag ctgggcacct ggccttaaag ttcttccctc tggcaatggc
     1741 tcatatccac cgttcagtag aactgaatcc ctctgtgaat catgtgaaca gcccaggaaa
     1801 tgcaatgtgt attgataact cctggttaga gttgtcctgt tagtgggcag aaaagggcaa
     1861 atcccatgat tctttcaaaa tctctcttgt cctgacagcc gatgtcatga atacatgaa
//
