LOCUS       NM_001414922            3780 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus glucosamine (N-acetyl)-6-sulfatase (Gns),
            transcript variant 1, mRNA.
ACCESSION   NM_001414922 XM_006241396
VERSION     NM_001414922.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Danyukova,T., Ariunbat,K., Thelen,M., Brocke-Ahmadinejad,N.,
            Mole,S.E. and Storch,S.
  TITLE     Loss of CLN7 results in depletion of soluble lysosomal proteins and
            impaired mTOR reactivation
  JOURNAL   Hum Mol Genet 27 (10), 1711-1722 (2018)
   PUBMED   29514215
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Sardiello,M., Annunziata,I., Roma,G. and Ballabio,A.
  TITLE     Sulfatases and sulfatase modifying factors: an exclusive and
            promiscuous relationship
  JOURNAL   Hum Mol Genet 14 (21), 3203-3217 (2005)
   PUBMED   16174644
REFERENCE   3  (bases 1 to 3780)
  AUTHORS   Zito,E., Fraldi,A., Pepe,S., Annunziata,I., Kobinger,G., Di
            Natale,P., Ballabio,A. and Cosma,M.P.
  TITLE     Sulphatase activities are regulated by the interaction of
            sulphatase-modifying factor 1 with SUMF2
  JOURNAL   EMBO Rep 6 (7), 655-660 (2005)
   PUBMED   15962010
  REMARK    Erratum:[EMBO Rep. 2016 Dec;17 (12 ):1901. PMID: 27909074]
REFERENCE   4  (bases 1 to 3780)
  AUTHORS   Mok,A., Cao,H. and Hegele,R.A.
  TITLE     Genomic basis of mucopolysaccharidosis type IIID (MIM 252940)
            revealed by sequencing of GNS encoding
            N-acetylglucosamine-6-sulfatase
  JOURNAL   Genomics 81 (1), 1-5 (2003)
   PUBMED   12573255
REFERENCE   5  (bases 1 to 3780)
  AUTHORS   Fuchs,W., Beck,M. and Kresse,H.
  TITLE     Intralysosomal formation and metabolic fate of N-acetylglucosamine
            6-sulfate from keratan sulfate
  JOURNAL   Eur J Biochem 151 (3), 551-556 (1985)
   PUBMED   3161730
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Dec 14, 2022 this sequence version replaced XM_006241396.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR22582416.576290.1,
                                           SRR22582424.1416875.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-270               JAXUCZ010000007.1  58774625-58774894
            271-330             JAXUCZ010000007.1  58780518-58780577
            331-537             JAXUCZ010000007.1  58783884-58784090
            538-603             JAXUCZ010000007.1  58785674-58785739
            604-702             JAXUCZ010000007.1  58786127-58786225
            703-870             JAXUCZ010000007.1  58787134-58787301
            871-953             JAXUCZ010000007.1  58789334-58789416
            954-1072            JAXUCZ010000007.1  58790368-58790486
            1073-1176           JAXUCZ010000007.1  58792364-58792467
            1177-1278           JAXUCZ010000007.1  58798027-58798128
            1279-1386           JAXUCZ010000007.1  58802001-58802108
            1387-1497           JAXUCZ010000007.1  58802765-58802875
            1498-1658           JAXUCZ010000007.1  58804211-58804371
            1659-3780           JAXUCZ010000007.1  58806529-58808650
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q22"
     gene            1..3780
                     /gene="Gns"
                     /note="glucosamine (N-acetyl)-6-sulfatase"
                     /db_xref="GeneID:299825"
                     /db_xref="RGD:1305877"
     exon            1..270
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..1737
                     /gene="Gns"
                     /EC_number="3.1.6.14"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; N-acetylglucosamine-6-sulfatase;
                     N-acetylglucosamine-6-sulfatase-like"
                     /codon_start=1
                     /product="N-acetylglucosamine-6-sulfatase isoform 1
                     precursor"
                     /protein_id="NP_001401851.1"
                     /db_xref="GeneID:299825"
                     /db_xref="RGD:1305877"
                     /translation="MRLPSAAGPGPGCPSRLPALLLLPLLSGCLGLVGAARRPNVLLL
                     LTDDQDAELGGMTPLKKTKALIGEKGMTFSSAYVPSALCCPSRASILTGKYPHNHHVV
                     NNTLEGNCSSKSWQKIQEPYTFPAILKLVCGYQTFFAGKYLNEYGAPDAGGLEHVPLG
                     WSYWYALEKNSKYYNYTLSINGKARRHGENYSVDYLTDVLANLSLDFLDYKSNSEPFF
                     MMISTPAPHSPWTAAPQYQKAFPNVIAPRNKNFNIHGTNKHWLIRQAKTPMTNSSIKF
                     LDDAFRRRWQTLLSVDDLVEKLVKRLDSTGELDNTYIFYTSDNGYHTGQFSLPIDKRQ
                     LYEFDIKVPLLVRGPGIKPNQTSKMLVSNIDLGPTILDLAGYDLNKTQMDGTSLLPIL
                     KGDGNLTWRSDVLVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDAYNNTYACVRTL
                     SSMWNLQYCEFDDQEVFVEVYNITADPDQITNIAKSIDPELLGKMNYRLMMLQSCSGP
                     TCRTPGVFDPGYRFDLRLMFSNHGSIRMRRFSKHPL"
     exon            271..330
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            331..537
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            538..603
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            604..702
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            703..870
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            871..953
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1072
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1176
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1278
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1386
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1497
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1658
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..3780
                     /gene="Gns"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctcacgtg atcgcgccta gggagagaaa gcttgacccg catctcctgt gctcacccgg
       61 tgtctctgcg cagctctcgg tttcccgcgg agcgtggcgg ccatgcggct cccgtccgca
      121 gctgggcccg ggccgggctg tcccagccgc ctgcccgcgc tgctgctact gccgctgctg
      181 agcggctgcc tggggctcgt gggggcggct cgcaggccca acgtgttgct gctgctcacg
      241 gacgaccagg acgcggagct cggcggcatg accccactaa agaaaaccaa ggccctcatc
      301 ggagagaagg ggatgacttt ctccagcgct tatgtgccca gtgctctctg ctgccccagc
      361 agagccagca tcctgactgg gaagtaccca cacaaccatc acgtcgtcaa caacactttg
      421 gaggggaact gcagtagcaa gtcctggcag aagatccagg agccatacac cttcccagcg
      481 atccttaagt tggtgtgcgg ctatcaaacc ttttttgctg ggaagtattt aaatgagtat
      541 ggagccccag atgcgggtgg actggagcac gtgcctctgg gctggagtta ctggtatgcc
      601 ctggaaaaga attctaaata ctataactac accctctcta tcaacgggaa ggcacgcaga
      661 catggcgaga actatagcgt ggactacctg acagacgttc tggccaatct ctccctggac
      721 ttcctggact acaagtccaa ctctgagcca ttcttcatga tgatctccac tccagcgccc
      781 cactcaccgt ggacagccgc acctcagtac cagaaggctt tccccaatgt catcgcaccg
      841 agaaacaaga acttcaacat ccatgggacg aacaagcact ggttaattag gcaagccaag
      901 actcccatga cgaattcctc aataaagttc ttagatgacg ccttcagaag gaggtggcaa
      961 actctgctct cagttgacga tcttgtggaa aaactggtga agagattgga ctcgactggt
     1021 gagctggaca acacgtacat cttctatacc tcagacaatg gctaccatac agggcagttc
     1081 tctttgccaa tagacaaacg acaactctat gagtttgata tcaaagttcc actgttggtt
     1141 cgagggcctg ggatcaaacc aaaccagaca agcaagatgc tggtctccaa tattgacttg
     1201 ggtcccacca tcctggacct cgctggctat gacctgaaca agacacagat ggacgggaca
     1261 tccctgctgc ccatcctgaa aggggacggt aacttaacct ggaggtcaga tgtcctggtg
     1321 gaatatcaag gagaaggccg taatgtcacc gacccgacct gcccttcttt gagtcctgga
     1381 gtgtctcaat gtttcccgga ctgtgtgtgt gaggacgcgt acaacaatac atatgcttgt
     1441 gtgaggacac tgtcatccat gtggaaccta cagtactgtg agttcgacga ccaggaggta
     1501 tttgtagaag tctataacat aactgcagat ccagaccaga tcaccaacat tgctaagagt
     1561 atagaccccg agcttttggg aaagatgaac tatcggctga tgatgctcca gtcctgttcc
     1621 gggccaacct gtcgcactcc gggggtcttt gaccctggat acaggtttga cctgcgcctc
     1681 atgttcagca atcatggcag catcagaatg agaaggttct caaagcatcc tctgtagcag
     1741 ctgtgcccag cctctgcagt tggatccttg cacacccttt tccgatgaag tagtcggagt
     1801 aagagcccag gcctggggga gctctggcct tgctgtttca agtcttgttt gaaaataacc
     1861 cagtcacctg acttccgtac tgtgaatctg cccccgtcag aagtggctta ccctgtctct
     1921 cctcaaatga caaccaccac tcctgaggtc tttgtcctct ccttgtccct tcatcttggc
     1981 tgggcacagt ataagagttc ccatgtggtc aagtctgaat ctgtaaaact cttctgataa
     2041 acggcaatac tttagggcag agagataaag cctttcggta tgtagctagc ccccaaatca
     2101 aaagcgctct ctagcatttc acactgaact cctcaccatc aaacacacac agccctgaga
     2161 agagctggag agaaagtacg gtcacaacat ccattgtggt gtggtaaatg tgcgacttgg
     2221 cacccaagct ctgccctaag atgccctcag cttttcagtg gctctaacca cagggagccc
     2281 agaagcacta tggagaagga accgccaggg ggagatgagc tagcagatcg ggctcttgga
     2341 tcagtgggct gaagggctgc acttagaagt gcctgtctgg acggttctcg agtatcaagg
     2401 ccacacacaa gtcatcctgc ttggctgcaa ttagctgaca ttgtcattga aaatattagt
     2461 gtttcctggt ggtgggaagg accggctgcc tcatcccacc tcccctgctc tcacagacat
     2521 tctattcctt aaagtccagt tccacagagg gctaagtgct ggcaggacag cagagtagag
     2581 ggcctggttc cagcctgtct aggccgtgag tgagtttagt gctacacagc ctctgccagc
     2641 tttaactttt aagacagagc tagaagtcac tttcagaatt ccagaggcca tgttggggtg
     2701 acagccactg agtttattcc tttacctcat gtgaccccag caactttggt gatacagaga
     2761 ggccagcagg catggaatgg acacctctgc tgtggctact tttagaggga aaaaaatttt
     2821 aagttttaaa ccaaaagtta caaaattgtg gctaagtggc atcattcact ccactcggat
     2881 tctaaacgtc atcagtgaaa tagtgaagtt ggcctcagtt gctcccaagt aacagtgagc
     2941 agctcctgcc aggcctcgga ccgtacgtcc tggtcttcag tctgaaggct gctcctgaga
     3001 gcaggtgacg tcagagtgac gacagccatc agctgcagta ggtcttccta cctccatgcc
     3061 ttcacgcaaa gcaggcgaca cagtgggtgg tgggacacga gatcctctgt acctcccagc
     3121 taaggagcgt ccctgcaaac atccaagtag acctcagctt gtaacagact gcagcaggcc
     3181 gcccacgttc tctggactca cagtttagac atctcctttg atgccccagt ccctgtgtgc
     3241 cgccgctctt caaaagggag agccattaaa accaacatgg agtgcagacg caccttacgc
     3301 atcccagttc taaaactgcc ctgcagacac agtttcaagg tggggtacac gttagagtct
     3361 gagttcagca agcggtactg tcacaaacac ctttcgactt gccagcgcca gggttttgta
     3421 tcaaaatagt aggagtggct atggaagtta cacctctgtc tgtgggaagt gttctcaaac
     3481 ctaggagcgt ctttatctta tttttttcta atgaaagatg gcagcccaga accctcaaga
     3541 aaaagcaagg agtatcagct gatcaccacc gggacaaatg ggtgtcctgg ggttctttag
     3601 ctcaccagca ctttccgctc aatggacttt gtaaattctt ttattagcat cacctgttgt
     3661 agaagctcct ctgccctgga cctgtcatct cagccgctca cttagaaagt gcccttcaac
     3721 ggaccctgtt cactgctgca cttttcaatg aattaaaact tacttctgtt ctagtgggaa
//
