LOCUS       NM_017084                988 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus glycine N-methyltransferase (Gnmt), mRNA.
ACCESSION   NM_017084
VERSION     NM_017084.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   3  (bases 1 to 988)
  AUTHORS   Wei,X., Das,J., Fragoza,R., Liang,J., Bastos de Oliveira,F.M.,
            Lee,H.R., Wang,X., Mort,M., Stenson,P.D., Cooper,D.N., Lipkin,S.M.,
            Smolka,M.B. and Yu,H.
  TITLE     A massively parallel pipeline to clone DNA variants and examine
            molecular phenotypes of human disease mutations
  JOURNAL   PLoS Genet 10 (12), e1004819 (2014)
   PUBMED   25502805
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 988)
  AUTHORS   Wei,J., Zhang,F., Zhang,Y., Cao,C., Li,X., Li,D., Liu,X., Yang,H.
            and Huang,L.
  TITLE     Proteomic investigation of signatures for geniposide-induced
            hepatotoxicity
  JOURNAL   J Proteome Res 13 (12), 5724-5733 (2014)
   PUBMED   25336395
  REMARK    GeneRIF: Data show that two biomarkers, glycine N-methyltransferase
            (GNMT) and glycogen phosphorylase (PYGL), were found to indicate
            early hepatic injuries.
REFERENCE   5  (bases 1 to 988)
  AUTHORS   Lu,S.C. and Mato,J.M.
  TITLE     S-adenosylmethionine in liver health, injury, and cancer
  JOURNAL   Physiol Rev 92 (4), 1515-1542 (2012)
   PUBMED   23073625
  REMARK    Review article
REFERENCE   6  (bases 1 to 988)
  AUTHORS   Pattanayek,R., Newcomer,M.E. and Wagner,C.
  TITLE     Crystal structure of apo-glycine N-methyltransferase (GNMT)
  JOURNAL   Protein Sci 7 (6), 1326-1331 (1998)
   PUBMED   9655336
REFERENCE   7  (bases 1 to 988)
  AUTHORS   Fu,Z., Hu,Y., Konishi,K., Takata,Y., Ogawa,H., Gomi,T., Fujioka,M.
            and Takusagawa,F.
  TITLE     Crystal structure of glycine N-methyltransferase from rat liver
  JOURNAL   Biochemistry 35 (37), 11985-11993 (1996)
   PUBMED   8810903
REFERENCE   8  (bases 1 to 988)
  AUTHORS   Yeo,E.J. and Wagner,C.
  TITLE     Tissue distribution of glycine N-methyltransferase, a major
            folate-binding protein of liver
  JOURNAL   Proc Natl Acad Sci U S A 91 (1), 210-214 (1994)
   PUBMED   8278367
REFERENCE   9  (bases 1 to 988)
  AUTHORS   Ogawa,H., Konishi,K., Takata,Y., Nakashima,H. and Fujioka,M.
  TITLE     Rat glycine methyltransferase. Complete amino acid sequence deduced
            from a cDNA clone and characterization of the genomic DNA
  JOURNAL   Eur J Biochem 168 (1), 141-151 (1987)
   PUBMED   2822402
REFERENCE   10 (bases 1 to 988)
  AUTHORS   Cook,R.J. and Wagner,C.
  TITLE     Glycine N-methyltransferase is a folate binding protein of rat
            liver cytosol
  JOURNAL   Proc Natl Acad Sci U S A 81 (12), 3631-3634 (1984)
   PUBMED   6587377
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from X06150.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X06150.1, EX491109.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="9"
                     /map="9q12"
     gene            1..988
                     /gene="Gnmt"
                     /note="glycine N-methyltransferase"
                     /db_xref="GeneID:25134"
                     /db_xref="RGD:2719"
     exon            1..210
                     /gene="Gnmt"
                     /inference="alignment:Splign:2.1.0"
     CDS             5..886
                     /gene="Gnmt"
                     /EC_number="2.1.1.20"
                     /note="folate-binding protein; glycine methyltransferase"
                     /codon_start=1
                     /product="glycine N-methyltransferase"
                     /protein_id="NP_058780.1"
                     /db_xref="GeneID:25134"
                     /db_xref="RGD:2719"
                     /translation="MVDSVYRTRSLGVAAEGIPDQYADGEAARVWQLYIGDTRSRTAE
                     YKAWLLGLLRQHGCHRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNR
                     RKEPAFDKWVIEEANWLTLDKDVPAGDGFDAVICLGNSFAHLPDSKGDQSEHRLALKN
                     IASMVRPGGLLVIDHRNYDYILSTGCAPPGKNIYYKSDLTKDITTSVLTVNNKAHMVT
                     LDYTVQVPGAGRDGAPGFSKFRLSYYPHCLASFTELVQEAFGGRCQHSVLGDFKPYRP
                     GQAYVPCYFIHVLKKTG"
     exon            211..338
                     /gene="Gnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            339..449
                     /gene="Gnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            450..592
                     /gene="Gnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            593..714
                     /gene="Gnmt"
                     /inference="alignment:Splign:2.1.0"
     exon            715..988
                     /gene="Gnmt"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      988
                     /gene="Gnmt"
                     /note="polyA site"
ORIGIN      
        1 caggatggtg gacagcgtgt accgtacccg ctccctgggg gtggcggccg aagggatccc
       61 cgaccagtat gcggatgggg aggccgcacg tgtgtggcag ctgtacatcg gggacacccg
      121 cagccgtact gcagagtaca aggcgtggtt gcttgggctg ctgcgccagc acgggtgcca
      181 ccgggtgctg gacgtggcct gtggcacagg agtggactcg attatgctgg tggaagaggg
      241 ctttagcgtc acgagtgtgg atgccagcga caagatgctg aaatacgcac tgaaggagcg
      301 ctggaaccgg aggaaggagc cagcctttga caagtgggtc attgaagaag ccaactggtt
      361 gactctggac aaagatgtgc cagcaggaga tggctttgac gctgtcatct gccttgggaa
      421 cagttttgct cacctgccgg acagcaaagg tgaccagagt gagcaccggc tggcgctaaa
      481 gaacatcgca agcatggtgc ggcccggggg cctgctggtc atcgaccacc gcaactacga
      541 ctacatcctc agcacgggct gtgcaccccc agggaagaac atctactata agagtgacct
      601 gaccaaggac attacgacgt cagtgctgac agtaaacaac aaagcccaca tggtaaccct
      661 ggactacaca gtgcaggtgc caggtgctgg cagagatggc gctcctggct tcagtaagtt
      721 tcggctctct tactacccac actgtttggc gtctttcacg gagttggtcc aagaagcctt
      781 tgggggcagg tgccagcaca gcgtcctggg tgacttcaag ccttacaggc ccggccaggc
      841 ctacgttccc tgctacttca tccacgtgct caagaagaca ggctgagcct ggctccggct
      901 cccaccctaa gaccatcgcc taccacagat attgcagaga tgtggggggc aggcaaacag
      961 ggagtcgaca atacagcctt cccttgcc
//
