LOCUS       NM_053765               2816 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus glucosamine
            (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (Gne), mRNA.
ACCESSION   NM_053765
VERSION     NM_053765.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Inim,M.D., Ibrahim,M.A., Isah,M.B. and Onyike,E.
  TITLE     Variations in the mRNA expression level of UDP-GlcNAc
            epimerase/ManNAc kinase and neuraminidase 1 genes in organs of type
            2 diabetic animals
  JOURNAL   Glycoconj J 38 (1), 129-134 (2021)
   PUBMED   33608771
  REMARK    GeneRIF: Variations in the mRNA expression level of UDP-GlcNAc
            epimerase/ManNAc kinase and neuraminidase 1 genes in organs of type
            2 diabetic animals.
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Weidemann,W., Reinhardt,A., Thate,A. and Horstkorte,R.
  TITLE     Biochemical characterization of the M712T-mutation of the
            UDP-N-acetylglucosamine 2-epimerase/N-acetyl-mannosaminekinase in
            hereditary inclusion body myopathy
  JOURNAL   Neuromuscul Disord 21 (12), 824-831 (2011)
   PUBMED   21873062
  REMARK    GeneRIF: M712T mutation of GNE gene does not affect GNE
            oligomerization but affects the solubility and posttranslational
            modification of the GNE protein.
REFERENCE   3  (bases 1 to 2816)
  AUTHORS   Kontou,M., Weidemann,W., Bauer,C., Reutter,W. and Horstkorte,R.
  TITLE     The key enzyme of sialic acid biosynthesis (GNE) promotes neurite
            outgrowth of PC12 cells
  JOURNAL   Neuroreport 19 (12), 1239-1242 (2008)
   PUBMED   18628673
  REMARK    GeneRIF: Data show that overexpression of GNE induces neurite
            outgrowth in PC12 cells, but does not lead to an increased
            biosynthesis of sialic acid.
REFERENCE   4  (bases 1 to 2816)
  AUTHORS   Ghaderi,D., Strauss,H.M., Reinke,S., Cirak,S., Reutter,W., Lucka,L.
            and Hinderlich,S.
  TITLE     Evidence for dynamic interplay of different oligomeric states of
            UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase by
            biophysical methods
  JOURNAL   J Mol Biol 369 (3), 746-758 (2007)
   PUBMED   17448495
  REMARK    GeneRIF: analysis of oligomeric states of UDP-N-acetylglucosamine
            2-epimerase/N-acetylmannosamine kinase by biophysical methods
REFERENCE   5  (bases 1 to 2816)
  AUTHORS   Weidemann,W., Stelzl,U., Lisewski,U., Bork,K., Wanker,E.E.,
            Hinderlich,S. and Horstkorte,R.
  TITLE     The collapsin response mediator protein 1 (CRMP-1) and the
            promyelocytic leukemia zinc finger protein (PLZF) bind to
            UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
            (GNE), the key enzyme of sialic acid biosynthesis
  JOURNAL   FEBS Lett 580 (28-29), 6649-6654 (2006)
   PUBMED   17118363
  REMARK    GeneRIF: The collapsin response mediator protein 1 and the
            promyelocytic leukemia zinc finger protein interact with
            UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase
            (GNE).
REFERENCE   6  (bases 1 to 2816)
  AUTHORS   Effertz,K., Hinderlich,S. and Reutter,W.
  TITLE     Selective loss of either the epimerase or kinase activity of
            UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase due
            to site-directed mutagenesis based on sequence alignments
  JOURNAL   J Biol Chem 274 (40), 28771-28778 (1999)
   PUBMED   10497249
REFERENCE   7  (bases 1 to 2816)
  AUTHORS   Keppler,O.T., Hinderlich,S., Langner,J., Schwartz-Albiez,R.,
            Reutter,W. and Pawlita,M.
  TITLE     UDP-GlcNAc 2-epimerase: a regulator of cell surface sialylation
  JOURNAL   Science 284 (5418), 1372-1376 (1999)
   PUBMED   10334995
REFERENCE   8  (bases 1 to 2816)
  AUTHORS   Horstkorte,R., Nohring,S., Wiechens,N., Schwarzkopf,M., Danker,K.,
            Reutter,W. and Lucka,L.
  TITLE     Tissue expression and amino acid sequence of murine
            UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase
  JOURNAL   Eur J Biochem 260 (3), 923-927 (1999)
   PUBMED   10103025
REFERENCE   9  (bases 1 to 2816)
  AUTHORS   Stasche,R., Hinderlich,S., Weise,C., Effertz,K., Lucka,L.,
            Moormann,P. and Reutter,W.
  TITLE     A bifunctional enzyme catalyzes the first two steps in
            N-acetylneuraminic acid biosynthesis of rat liver. Molecular
            cloning and functional expression of UDP-N-acetyl-glucosamine
            2-epimerase/N-acetylmannosamine kinase
  JOURNAL   J Biol Chem 272 (39), 24319-24324 (1997)
   PUBMED   9305888
REFERENCE   10 (bases 1 to 2816)
  AUTHORS   Hinderlich,S., Stasche,R., Zeitler,R. and Reutter,W.
  TITLE     A bifunctional enzyme catalyzes the first two steps in
            N-acetylneuraminic acid biosynthesis of rat liver. Purification and
            characterization of UDP-N-acetylglucosamine
            2-epimerase/N-acetylmannosamine kinase
  JOURNAL   J Biol Chem 272 (39), 24313-24318 (1997)
   PUBMED   9305887
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Nov 27, 2020 this sequence version replaced NM_053765.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062011.1, SRR26360172.231509.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760434,
                                           SAMN12840105 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-297               JAXUCZ010000005.1  63092889-63093185   c
            298-503             JAXUCZ010000005.1  63088265-63088470   c
            504-955             JAXUCZ010000005.1  63083440-63083891   c
            956-1108            JAXUCZ010000005.1  63078913-63079065   c
            1109-1321           JAXUCZ010000005.1  63076716-63076928   c
            1322-1409           JAXUCZ010000005.1  63071498-63071585   c
            1410-1620           JAXUCZ010000005.1  63070322-63070532   c
            1621-1750           JAXUCZ010000005.1  63067854-63067983   c
            1751-1972           JAXUCZ010000005.1  63067321-63067542   c
            1973-2155           JAXUCZ010000005.1  63065679-63065861   c
            2156-2272           JAXUCZ010000005.1  63064523-63064639   c
            2273-2816           JAXUCZ010000005.1  63062953-63063496   c
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q22"
     gene            1..2816
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /note="glucosamine
                     (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase"
                     /db_xref="GeneID:114711"
                     /db_xref="RGD:69239"
     exon            1..297
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            298..503
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
     CDS             340..2508
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /EC_number="2.7.1.60"
                     /EC_number="3.2.1.183"
                     /note="UDP-GlcNAc-2-epimerase/ManAc kinase;
                     UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine
                     kinase"
                     /codon_start=1
                     /product="bifunctional UDP-N-acetylglucosamine
                     2-epimerase/N-acetylmannosamine kinase"
                     /protein_id="NP_446217.1"
                     /db_xref="GeneID:114711"
                     /db_xref="RGD:69239"
                     /translation="MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVV
                     VLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRL
                     KPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCC
                     TRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKCKDYIVAL
                     QHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNF
                     RAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHV
                     RDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPV
                     KENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERISLIL
                     QMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTKLIQEWNSVDLRTPLSDTL
                     HLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGSSFCAAE
                     LGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGA
                     LHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVRDV
                     IRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIH"
     exon            504..955
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1108
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1321
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1409
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1620
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1750
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1972
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            1973..2155
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            2156..2272
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
     exon            2273..2816
                     /gene="Gne"
                     /gene_synonym="Uae1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tagcggaagc cgcggggtgc gcttgccggg gcctgccccg tggacccgct ggacccgagc
       61 ctgcagagtg ccgaggtggc gccggaggga actaacccgg cggaggccac gagtcagtcg
      121 gtgactcacg agcatcgtgc caggctctga caccatcgct gcagttgtgg gcctcgcgct
      181 gtgtgcccgg gttctgcacc gcagggcgcc tctgctgtcc cggtgcgttg ctccggcgtg
      241 ggatcccgga gctgtcaggc tcagcgcgag ggtgaatggg gaccggggcg tgcaaaggaa
      301 ctctatttta aaaaactctc aagtaaaaaa aagcaagtca tggagaagaa cgggaataac
      361 cggaagcttc gggtttgcgt tgccacctgc aaccgagccg attactccaa actggccccc
      421 atcatgttcg gcattaagac ggagcctgcg ttcttcgagc tcgacgtggt ggtgctgggc
      481 tctcacctga tcgacgacta cggaaacaca taccgcatga ttgagcagga cgactttgac
      541 atcaacacca ggctacacac gattgttaga ggggaagacg aagcagccat ggtagagtca
      601 gtgggcctag cgctagtgaa gctaccggat gtcctcaacc gcctgaagcc tgacatcatg
      661 attgttcacg gagaccgatt tgacgccctc gctctggcta catctgctgc cctgatgaac
      721 atccgcatcc ttcacattga aggaggagag gtcagtggga ctattgatga ctctatcaga
      781 cacgccataa caaaactggc tcactaccac gtgtgctgca ccaggagtgc agagcaacac
      841 ctgatctcca tgtgtgagga ccacgaccgc atccttttgg ccggctgccc ttcctatgac
      901 aaactgctct cagccaagaa taaagactat atgagcatca ttcggatgtg gctaggtgat
      961 gatgtaaaat gtaaagatta cattgttgcc ctgcaacacc cggtgaccac cgacattaag
     1021 cattccataa agatgttcga actgacactg gatgctctta tctcatttaa caagaggacc
     1081 ctagttctgt ttccaaatat cgatgcaggc agcaaggaga tggttcgagt gatgcggaag
     1141 aagggcatcg agcatcaccc caatttccgc gcagtcaagc acgtcccgtt tgaccagttc
     1201 attcagctgg tcgcccacgc tggctgcatg attgggaata gcagctgtgg agtgcgtgag
     1261 gttggcgcct ttggaacccc tgtgatcaac ctgggcacgc ggcagatagg aagagaaacg
     1321 ggggagaatg ttcttcatgt ccgggatgct gacacccaag acaaaatatt acaagcacta
     1381 cacctccagt tcggtaaaca gtacccttgc tcaaagatat atggggatgg aaatgctgtt
     1441 ccaaggattt taaagtttct caaatccatc gaccttcaag agccactaca gaagaaattc
     1501 tgcttccctc ccgtgaagga gaacatctct caggatattg accatatcct cgaaactctg
     1561 agtgccttgg ctgttgatct cggggggacg aatctgagag tggcgatagt tagcatgaag
     1621 ggtgaaatag ttaagaagta cacccagttc aatcctaaaa cctatgagga aaggattagt
     1681 ctaatcctgc agatgtgtgt ggaagcggca gcagaagccg tgaagctcaa ttgcagaatt
     1741 ctgggagtag gcatctccac aggtggccgt gtgaatcccc aggaaggagt tgtgctgcac
     1801 tcgaccaagc tgatacagga gtggaactct gtggacctca ggacaccact ctccgacacc
     1861 ctgcatctcc ccgtgtgggt ggacaacgac ggcaactgcg ctgccatggc ggagaggaag
     1921 tttggccaag gaaaaggaca ggagaacttt gtgacgctca tcacagggac agggatcggt
     1981 gggggaatca tccaccagca cgagctgatc cacggcagct ccttctgtgc ggcagagctt
     2041 ggccacctcg tggtgtctct ggatggtcct gactgctcct gtggaagcca tgggtgcatt
     2101 gaagcctacg cctctggaat ggccttgcag agggaagcaa agaagctcca cgacgaggac
     2161 ctgctcttgg tggaagggat gtcagtgcca aaagacgaag ctgtgggcgc cctccatctc
     2221 atccaagccg ccaagctggg caacgtgaag gcccagagca tcttacggac agctggaact
     2281 gctttgggac tcggagttgt gaatatcctc cacactatga atccttccct ggtgatcctg
     2341 tctggagtcc tggctagtca ctacatccac attgtgaggg acgtcatccg ccagcaagcc
     2401 ctgtcctccg tgcaggatgt ggatgtagtg gtttcagact tggttgaccc ggccctgctt
     2461 ggtgcggcca gcatggttct ggactacacg acccgcagga tccactaggc ctcctgggaa
     2521 tagacctgga ctgagaccca agagctactg agtggaacca cgctctctta gatcagtatt
     2581 tcttcaaagg ccaatgtggg aggctgcgga gccagctcag tggtcaagag cctaaactgc
     2641 tcttgccaaa gacccaagtt cagttcccag cacccatgtc aggcactcaa ctgcctgaaa
     2701 gccaagctcc aggggatcca gtgcctcagc tgcctcaggc atctgcactc aaatgtacat
     2761 agtcctctcc cagaatacac gtaaattaaa ataaatctta ttttttaaaa ggcaaa
//
