LOCUS       NM_013106               3069 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus G protein subunit alpha i3 (Gnai3), mRNA.
ACCESSION   NM_013106
VERSION     NM_013106.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Sarkar,O., Li,Y. and Anand-Srivastava,M.B.
  TITLE     Nitric oxide attenuates overexpression of Gialpha proteins in
            vascular smooth muscle cells from SHR: Role of ROS and ROS-mediated
            signaling
  JOURNAL   PLoS One 12 (7), e0179301 (2017)
   PUBMED   28692698
  REMARK    GeneRIF: Nitric oxide decreased the enhanced expression of
            Gialpha-2/3 proteins and hyperproliferation of Vascular smooth
            muscle cells from spontaneously hypertensive rats by
            cGMP-independent mechanism.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Parag-Sharma,K., Leyme,A., DiGiacomo,V., Marivin,A., Broselid,S.
            and Garcia-Marcos,M.
  TITLE     Membrane Recruitment of the Non-receptor Protein GIV/Girdin
            (Galpha-interacting, Vesicle-associated Protein/Girdin) Is
            Sufficient for Activating Heterotrimeric G Protein Signaling
  JOURNAL   J Biol Chem 291 (53), 27098-27111 (2016)
   PUBMED   27864364
REFERENCE   3  (bases 1 to 3069)
  AUTHORS   Ali El-Basyuni,Y., Li,Y. and Anand-Srivastava,M.B.
  TITLE     Knockdown of Inhibitory Guanine Nucleotide Binding Protein
            Gialpha-2 by Antisense Oligodeoxynucleotides Attenuates the
            Development of Hypertension and Tachycardia in Spontaneously
            Hypertensive Rats
  JOURNAL   J Am Heart Assoc 5 (11), e004594 (2016)
   PUBMED   27912212
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3069)
  AUTHORS   Gupta,V., Bhandari,D., Leyme,A., Aznar,N., Midde,K.K., Lo,I.C.,
            Ear,J., Niesman,I., Lopez-Sanchez,I., Blanco-Canosa,J.B., von
            Zastrow,M., Garcia-Marcos,M., Farquhar,M.G. and Ghosh,P.
  TITLE     GIV/Girdin activates Galphai and inhibits Galphas via the same
            motif
  JOURNAL   Proc Natl Acad Sci U S A 113 (39), E5721-E5730 (2016)
   PUBMED   27621449
REFERENCE   5  (bases 1 to 3069)
  AUTHORS   Wang,X.Y., Fan,X.S., Cai,L., Liu,S., Cong,X.F. and Chen,X.
  TITLE     Lysophosphatidic acid rescues bone mesenchymal stem cells from
            hydrogen peroxide-induced apoptosis
  JOURNAL   Apoptosis 20 (3), 273-284 (2015)
   PUBMED   25633408
REFERENCE   6  (bases 1 to 3069)
  AUTHORS   De Vries,L., Mousli,M., Wurmser,A. and Farquhar,M.G.
  TITLE     GAIP, a protein that specifically interacts with the trimeric G
            protein G alpha i3, is a member of a protein family with a highly
            conserved core domain
  JOURNAL   Proc Natl Acad Sci U S A 92 (25), 11916-11920 (1995)
   PUBMED   8524874
REFERENCE   7  (bases 1 to 3069)
  AUTHORS   Cadrin,M., McFarlane-Anderson,N. and Begin-Heick,N.
  TITLE     Identification and localization of G(i) alpha 3 in the clonal
            adipocyte cell lines HGFu and Ob17
  JOURNAL   Biochem Cell Biol 71 (11-12), 518-521 (1993)
   PUBMED   8192889
REFERENCE   8  (bases 1 to 3069)
  AUTHORS   Linder,M.E., Ewald,D.A., Miller,R.J. and Gilman,A.G.
  TITLE     Purification and characterization of Go alpha and three types of Gi
            alpha after expression in Escherichia coli
  JOURNAL   J Biol Chem 265 (14), 8243-8251 (1990)
   PUBMED   2159473
REFERENCE   9  (bases 1 to 3069)
  AUTHORS   Itoh,H., Toyama,R., Kozasa,T., Tsukamoto,T., Matsuoka,M. and
            Kaziro,Y.
  TITLE     Presence of three distinct molecular species of Gi protein alpha
            subunit. Structure of rat cDNAs and human genomic DNAs
  JOURNAL   J Biol Chem 263 (14), 6656-6664 (1988)
   PUBMED   2834384
REFERENCE   10 (bases 1 to 3069)
  AUTHORS   Jones,D.T. and Reed,R.R.
  TITLE     Molecular cloning of five GTP-binding protein cDNA species from rat
            olfactory neuroepithelium
  JOURNAL   J Biol Chem 262 (29), 14241-14249 (1987)
   PUBMED   2820999
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000002.1.
            
            On Nov 26, 2020 this sequence version replaced NM_013106.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M20713.1, FQ222046.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760393 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               JAXUCZ010000002.1  198468674-198468874 c
            202-244             JAXUCZ010000002.1  198446703-198446745 c
            245-386             JAXUCZ010000002.1  198446453-198446594 c
            387-544             JAXUCZ010000002.1  198442158-198442315 c
            545-673             JAXUCZ010000002.1  198440010-198440138 c
            674-803             JAXUCZ010000002.1  198436959-198437088 c
            804-957             JAXUCZ010000002.1  198436416-198436569 c
            958-1167            JAXUCZ010000002.1  198432915-198433124 c
            1168-3069           JAXUCZ010000002.1  198430920-198432821 c
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q34"
     gene            1..3069
                     /gene="Gnai3"
                     /gene_synonym="GIP3A"
                     /note="G protein subunit alpha i3"
                     /db_xref="GeneID:25643"
                     /db_xref="RGD:2714"
     exon            1..201
                     /gene="Gnai3"
                     /gene_synonym="GIP3A"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..1148
                     /gene="Gnai3"
                     /gene_synonym="GIP3A"
                     /note="guanine nucleotide-binding protein G(k) subunit
                     alpha; guanine nucleotide binding protein (G protein),
                     alpha inhibiting 3; g(i) alpha-3; Guanine nucleotide
                     binding, protein, alpha inhibiting polypeptide 3; guanine
                     nucleotide binding protein (G protein), alpha inhibiting
                     activity polypeptide 3; guanine nucleotide binding
                     protein, alpha inhibiting 3; guanine nucleotide-binding
                     protein G(i) subunit alpha"
                     /codon_start=1
                     /product="guanine nucleotide-binding protein G(i) subunit
                     alpha-3"
                     /protein_id="NP_037238.1"
                     /db_xref="GeneID:25643"
                     /db_xref="RGD:2714"
                     /translation="MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGES
                     GKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARAD
                     DARQLFVLAGSAEEGVMTSELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLD
                     RISQTNYIPTQQDVLRTRVKTTGIVETHFTFKELYFKMFDVGGQRSERKKWIHCFEGV
                     TAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEE
                     KIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEVYTHFTCATDTKNVQF
                     VFDAVTDVIIKNNLKECGLY"
     exon            202..244
                     /gene="Gnai3"
                     /gene_synonym="GIP3A"
                     /inference="alignment:Splign:2.1.0"
     exon            245..386
                     /gene="Gnai3"
                     /gene_synonym="GIP3A"
                     /inference="alignment:Splign:2.1.0"
     exon            387..544
                     /gene="Gnai3"
                     /gene_synonym="GIP3A"
                     /inference="alignment:Splign:2.1.0"
     exon            545..673
                     /gene="Gnai3"
                     /gene_synonym="GIP3A"
                     /inference="alignment:Splign:2.1.0"
     exon            674..803
                     /gene="Gnai3"
                     /gene_synonym="GIP3A"
                     /inference="alignment:Splign:2.1.0"
     exon            804..957
                     /gene="Gnai3"
                     /gene_synonym="GIP3A"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1167
                     /gene="Gnai3"
                     /gene_synonym="GIP3A"
                     /inference="alignment:Splign:2.1.0"
     exon            1168..3069
                     /gene="Gnai3"
                     /gene_synonym="GIP3A"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagaagacgc gtctcagccg gtgtgtcgcg gttcccgcgg tgtgtgagtg agccagggcc
       61 cggtcccctc tccggccgcc gtcatgggct gcacgttgag cgccgaggac aaggcggcgg
      121 tggagcggag taagatgatc gaccgcaact tgcgggagga cggagagaaa gcggccaaag
      181 aagtgaagct gctgctgctc ggcgctggag aatctggtaa aagtactatt gtgaaacaga
      241 tgaaaatcat tcatgaggat ggctattccg aggacgagtg taagcagtat aaagttgtcg
      301 tctacagcaa taccattcag tccatcattg caatcataag agccatggga cggttgaaga
      361 ttgattttgg ggaagctgcc agagcggatg atgcccgaca gttatttgtt ttagctggca
      421 gtgctgagga aggagtcatg acttcagaac tagcaggcgt gattaaacgt ttatggcgag
      481 atggcggggt gcaggcatgc ttcagcaggt ccagggaata tcagctcaat gattctgctt
      541 catattacct aaatgatttg gatagaatat cccagaccaa ctacattcca actcagcaag
      601 atgttcttcg gacgagagtg aagactacag gcattgtgga gacccacttc accttcaagg
      661 agctctactt caaaatgttt gatgtaggtg gccaaagatc cgaacggaaa aagtggatcc
      721 actgttttga gggagtgaca gcaattatct tttgtgtggc tctcagtgat tacgaccttg
      781 ttctggctga ggacgaggaa atgaaccgaa tgcatgaaag catgaaattg tttgacagca
      841 tttgtaacaa caaatggttt acagacactt caatcattct cttccttaat aagaaagacc
      901 tttttgagga aaaaataaag aggagtccat taacaatctg ttatccagaa tacacaggtt
      961 ccaatacgta cgaagaggca gctgcttaca tccagtgcca gtttgaagat ctgaaccgaa
     1021 gaaaggacac caaggaggtc tacactcact ttacctgtgc cactgacacc aaaaacgtgc
     1081 agtttgtttt tgatgctgtt acagatgtca tcattaaaaa caacttaaag gaatgtgggc
     1141 tttactgaga ggatggcata gtaaaagcta ctacagggag gaatgttgag accagacatc
     1201 atctactgtc tcttgggtca gcgacaagca tgacaggacc aaggaatggc aacagcatgc
     1261 agaatcttta gcacaatctt ctgtattagg aaatgtttaa ttggcatgag aagagatgat
     1321 cgagtcagac atgaaattgg acaactgtaa agtatgaccg gatcatcagg gcaccgcttg
     1381 gactttaaat ctcaatgttt agggcatatt gaagtcgagg tgctgcattc cagaacttaa
     1441 acttgtagct tactgttccc cgtcttttta acaaatgacc agtagttaat ttctaaggtt
     1501 tttctcatca agagaacaat acctaaaaac tcttacttgt ttgcaaaaga atcttctggg
     1561 gactcagtct taactatgca cacaatgtag ccggacatct tgaaatattc ctcttagtag
     1621 gaactgtttg tttttaactc ttggaatggt caggagtaat attccacagc taaaaatctt
     1681 cctggttatt gggactatac taatagagct ttttggaatg aagtttatgt tactgtcctg
     1741 tttaaagtat cacaatggct tctaaacagc attcacttcg gagagctgta cagtaagatt
     1801 ctagctctcg tttcttaagc ttacggttgg agttaacctt gtttgtgctg attaccaaat
     1861 tactagatga atactgatgt aatatgataa actgctcttg ggtacagaca cacgcaggat
     1921 agctgtcagt cagagacgca gactgctaag gtgagaagca gtgacttgct gctacagatc
     1981 taccgaggtg gccacactga tttgcctgct cgtctcaggc gtgtgggttg tatgcggtgc
     2041 cccttgtttt ccatctttgt ggcctttttt gctaggagac gtctaagagt ataacaactg
     2101 tgcatgcctt tcctttgtaa atgtgctgcc aaatccctgc ttgctgtcat tgtcactgta
     2161 tcgatggtaa ctgcatagcg gcccttgctg tgttcttttt ctaagacttg cattctggga
     2221 ctctgttcca ccttgtcacg tgtacagact ttaatctgtt acagctggca gcagtattaa
     2281 cgcggtgagt aaagagccca ggattgctcc tgtttgtaac cgatcctttc tggaagccct
     2341 gccttctgtt tctttttgcc cctgctgagt gttctttagc ctttgagagt gggtttaggt
     2401 agaaaagtgt ttttgaaagt ctagttcctt gatgtaatag tgatatcaaa tctagggctg
     2461 ggggctttat gtccagtctg tgcaagtcat acaagtgtcc gatgtgtaca gtttgcttgc
     2521 gctgagagct tcagtcctct tgtacagact gtatcaaatg tcattaaaca ctgagtggac
     2581 cactgaggga cggcaaacag aggatgcatt ttgagcattt cagatcagta gtttgatcag
     2641 tgccttttga ggtccagtct ttgtggattg gatccgttca tcttttgatg ttacctccta
     2701 ctcatttgaa agaaggttgg tggtcagaac tgctttggga agcaagccac ggtgatcagt
     2761 gccttcattg gcagctttta ccatttttaa ttcttacgtg tctttgcgaa atcattatta
     2821 tgcagacagt ataactgtta tcttacaaat atttccatag ggctaagaaa gtatcacttg
     2881 aaagaaaagt attgatttca atcagcgatt gactcaattc ccacagtgta ggggttaata
     2941 agtcttactc tcaatttatt gttcctttgt tgtattctgt aatataagaa catctgggaa
     3001 tgcgaactag tggaatatgt tttcataatt tttctatact ttgggtttaa taaaatggtg
     3061 ttgataatc
//
