LOCUS       NM_031035               2218 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus G protein subunit alpha i2 (Gnai2), mRNA.
ACCESSION   NM_031035
VERSION     NM_031035.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Sarkar,O., Li,Y. and Anand-Srivastava,M.B.
  TITLE     Nitric oxide attenuates overexpression of Gialpha proteins in
            vascular smooth muscle cells from SHR: Role of ROS and ROS-mediated
            signaling
  JOURNAL   PLoS One 12 (7), e0179301 (2017)
   PUBMED   28692698
  REMARK    GeneRIF: Nitric oxide decreased the enhanced expression of
            Gialpha-2/3 proteins and hyperproliferation of Vascular smooth
            muscle cells from spontaneously hypertensive rats by
            cGMP-independent mechanism.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Ali El-Basyuni,Y., Li,Y. and Anand-Srivastava,M.B.
  TITLE     Knockdown of Inhibitory Guanine Nucleotide Binding Protein
            Gialpha-2 by Antisense Oligodeoxynucleotides Attenuates the
            Development of Hypertension and Tachycardia in Spontaneously
            Hypertensive Rats
  JOURNAL   J Am Heart Assoc 5 (11), e004594 (2016)
   PUBMED   27912212
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2218)
  AUTHORS   Wang,X.Y., Fan,X.S., Cai,L., Liu,S., Cong,X.F. and Chen,X.
  TITLE     Lysophosphatidic acid rescues bone mesenchymal stem cells from
            hydrogen peroxide-induced apoptosis
  JOURNAL   Apoptosis 20 (3), 273-284 (2015)
   PUBMED   25633408
REFERENCE   4  (bases 1 to 2218)
  AUTHORS   Chiasserini,D., van Weering,J.R., Piersma,S.R., Pham,T.V.,
            Malekzadeh,A., Teunissen,C.E., de Wit,H. and Jimenez,C.R.
  TITLE     Proteomic analysis of cerebrospinal fluid extracellular vesicles: a
            comprehensive dataset
  JOURNAL   J Proteomics 106, 191-204 (2014)
   PUBMED   24769233
REFERENCE   5  (bases 1 to 2218)
  AUTHORS   Moller,D., Kling,R.C., Skultety,M., Leuner,K., Hubner,H. and
            Gmeiner,P.
  TITLE     Functionally selective dopamine D, D receptor partial agonists
  JOURNAL   J Med Chem 57 (11), 4861-4875 (2014)
   PUBMED   24831693
REFERENCE   6  (bases 1 to 2218)
  AUTHORS   Lerman,B.B., Dong,B., Stein,K.M., Markowitz,S.M., Linden,J. and
            Catanzaro,D.F.
  TITLE     Right ventricular outflow tract tachycardia due to a somatic cell
            mutation in G protein subunitalphai2
  JOURNAL   J Clin Invest 101 (12), 2862-2868 (1998)
   PUBMED   9637720
REFERENCE   7  (bases 1 to 2218)
  AUTHORS   Rudolph,U., Spicher,K. and Birnbaumer,L.
  TITLE     Adenylyl cyclase inhibition and altered G protein subunit
            expression and ADP-ribosylation patterns in tissues and cells from
            Gi2 alpha-/-mice
  JOURNAL   Proc Natl Acad Sci U S A 93 (8), 3209-3214 (1996)
   PUBMED   8622915
REFERENCE   8  (bases 1 to 2218)
  AUTHORS   Linder,M.E., Ewald,D.A., Miller,R.J. and Gilman,A.G.
  TITLE     Purification and characterization of Go alpha and three types of Gi
            alpha after expression in Escherichia coli
  JOURNAL   J Biol Chem 265 (14), 8243-8251 (1990)
   PUBMED   2159473
REFERENCE   9  (bases 1 to 2218)
  AUTHORS   Jones,D.T. and Reed,R.R.
  TITLE     Molecular cloning of five GTP-binding protein cDNA species from rat
            olfactory neuroepithelium
  JOURNAL   J Biol Chem 262 (29), 14241-14249 (1987)
   PUBMED   2820999
REFERENCE   10 (bases 1 to 2218)
  AUTHORS   Itoh,H., Kozasa,T., Nagata,S., Nakamura,S., Katada,T., Ui,M.,
            Iwai,S., Ohtsuka,E., Kawasaki,H., Suzuki,K. et al.
  TITLE     Molecular cloning and sequence determination of cDNAs for alpha
            subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go
            from rat brain
  JOURNAL   Proc Natl Acad Sci U S A 83 (11), 3776-3780 (1986)
   PUBMED   3086867
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CO394955.1, BC078806.1 and CR465951.1.
            
            On Oct 25, 2007 this sequence version replaced NM_031035.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC078806.1, M17528.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                CO394955.1         1-48
            49-1781             BC078806.1         1-1733
            1782-2211           BC078806.1         1735-2164
            2212-2218           CR465951.1         1-7                 c
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q32"
     gene            1..2218
                     /gene="Gnai2"
                     /gene_synonym="Galphai2; Hg1c"
                     /note="G protein subunit alpha i2"
                     /db_xref="GeneID:81664"
                     /db_xref="RGD:620243"
     exon            1..270
                     /gene="Gnai2"
                     /gene_synonym="Galphai2; Hg1c"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..1220
                     /gene="Gnai2"
                     /gene_synonym="Galphai2; Hg1c"
                     /note="guanine nucleotide-binding protein G(i), alpha-2
                     subunit; G alpha inhibitory type 2 (a); G alpha inhibitory
                     type 2 (b); adenylate cyclase-inhibiting G alpha protein;
                     GTP-binding protein (G-alpha-i2); guanine nucleotide
                     binding protein, alpha inhibiting 2; guanine nucleotide
                     binding protein (G protein), alpha inhibiting 2; guanine
                     nucleotide binding protein (G protein), alpha inhibiting
                     activity polypeptide 2; heterotrimeric guanine
                     nucleotide-binding protein 1c"
                     /codon_start=1
                     /product="guanine nucleotide-binding protein G(i) subunit
                     alpha-2"
                     /protein_id="NP_112297.1"
                     /db_xref="GeneID:81664"
                     /db_xref="RGD:620243"
                     /translation="MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGES
                     GKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPQRAD
                     DARQLFALSCAAEEQGMLPEDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDL
                     ERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEG
                     VTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFE
                     EKITQSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQ
                     FVFDAVTDVIIKNNLKDCGLF"
     exon            271..313
                     /gene="Gnai2"
                     /gene_synonym="Galphai2; Hg1c"
                     /inference="alignment:Splign:2.1.0"
     exon            314..455
                     /gene="Gnai2"
                     /gene_synonym="Galphai2; Hg1c"
                     /inference="alignment:Splign:2.1.0"
     exon            456..616
                     /gene="Gnai2"
                     /gene_synonym="Galphai2; Hg1c"
                     /inference="alignment:Splign:2.1.0"
     exon            617..745
                     /gene="Gnai2"
                     /gene_synonym="Galphai2; Hg1c"
                     /inference="alignment:Splign:2.1.0"
     exon            746..875
                     /gene="Gnai2"
                     /gene_synonym="Galphai2; Hg1c"
                     /inference="alignment:Splign:2.1.0"
     exon            876..1029
                     /gene="Gnai2"
                     /gene_synonym="Galphai2; Hg1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1243
                     /gene="Gnai2"
                     /gene_synonym="Galphai2; Hg1c"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..2217
                     /gene="Gnai2"
                     /gene_synonym="Galphai2; Hg1c"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcggcggccg ggaaggcgcc tcccgcagtc gctcggaact gcggacctga gagcttcccg
       61 cagagggccg gcggtgggag cggagtgggt cgggcggggc cgagccgggc cgtgggccgt
      121 gtgggggcca ggccgggccg gcggacggca ggatgggctg caccgtgagc gccgaggaca
      181 aggcggcagc cgagcgctct aagatgatcg acaagaacct gcgggaggac ggcgagaagg
      241 cggcacggga ggtgaagttg cttctgttag gtgctggaga atcagggaag agcaccatcg
      301 tcaagcagat gaagatcatc cacgaggatg gctactcaga ggaggagtgc cggcagtacc
      361 gtgcggttgt ctacagcaac accatccagt ctatcatggc catcgtcaaa gccatgggca
      421 acctgcagat cgactttgct gacccccagc gtgcggatga tgccaggcag ctgttcgcac
      481 tgtcctgtgc tgccgaggag caaggcatgc ttccggaaga cctgtcgggc gtcatccgga
      541 ggctctgggc tgaccatggt gtgcaagcct gctttggccg ctcacgggaa tatcaactca
      601 atgactcagc cgcttactac ctgaatgacc tggagcgcat agcacagagt gactatatcc
      661 ctacacagca ggatgtgctg cggacccgtg tgaagaccac aggcatcgtc gaaacacact
      721 tcaccttcaa ggacttacac ttcaagatgt ttgatgtggg tggtcagcga tctgagcgga
      781 agaagtggat ccactgcttt gagggtgtca cggccatcat cttctgtgtc gccttgagcg
      841 cgtacgactt ggtgctggct gaggatgagg agatgaatcg catgcatgag agcatgaagc
      901 tgtttgatag catctgcaat aataagtggt tcacagacac ctccatcatc ctcttcctca
      961 acaagaagga cctgtttgaa gagaagatca cacagagccc cctgaccatc tgtttccctg
     1021 agtacacagg ggccaacaag tatgacgagg cagccagcta catccagagc aagtttgagg
     1081 acctgaataa acgcaaagac accaaggaga tctacacgca cttcacatgc gccaccgaca
     1141 ccaagaacgt gcagtttgtg tttgatgccg tcactgacgt catcatcaag aacaacctga
     1201 aggactgtgg cctcttctga ggggcagtgg gcctggcagg atgggccacc gctgactctg
     1261 ctccccacta cctgtgagga agatgggggc aagaagacca tgctccctgc ctgttccccc
     1321 agctgcttct cccctgtctc ttctctctgt tctcagctcc cctgtcccct ccctcggctc
     1381 tagacttggg ggaggggttg ccacaggcct ccctgtctaa aacccacctt tgtctgaggt
     1441 gccgggagtg gccatggtac ctccttactg ggcatccatt cgggttttct aaccattgtc
     1501 ttgttctggg gtgagcgggg agcgcatgca gagtttccca aggcctatgt ctggaggagt
     1561 accaattcct ccagcctaga cccctggctt tgtccaacac cagccctgac ccaagtccaa
     1621 atgtttacag ggagcctcct gcctacccca ctctctgccg cttggaggcc ccaaaggaaa
     1681 aagcacaaga agcgtgagag acaccaccat tcctggagac aaagcccacc tgctcattct
     1741 cgtagcttta aaaaaaagga aaaggaaagg agaaaaaaaa aggaaaactg caaatctaga
     1801 aaacttttta gagaaaccta tttaaaactg tcaggtcctg accagcatcc ctccccccgc
     1861 cccccagccc ctttccagtg attccgtgcc ttgagtgttt ctgcctgttt acacccatcc
     1921 ctctttgggc ggccccctgc cctgccctcc acggaattgg attccaaggg ctgttccaga
     1981 caactgccaa tgtcactgag ggccctgccc tgaagccctg ggcccaggct ccattaacct
     2041 aaatgtagct ccttagcgct aatctaggaa ccgccgctgc ctgctgaggg tcaggcccct
     2101 cacgccctcg ccccaggccc gggacctcca gcgttgaaca cttccttgct tttttcacat
     2161 gttttatgga attgttcaca tgatttgaaa taataaaatg tagaaagaaa acaagaaa
//
