LOCUS       NM_013145               4140 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus G protein subunit alpha i1 (Gnai1), mRNA.
ACCESSION   NM_013145 XM_039107131
VERSION     NM_013145.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Andhirka,S.K., Vignesh,R. and Aradhyam,G.K.
  TITLE     The nucleotide-free state of heterotrimeric G proteins
            alpha-subunit adopts a highly stable conformation
  JOURNAL   FEBS J 284 (15), 2464-2481 (2017)
   PUBMED   28627018
  REMARK    GeneRIF: Data suggest that Gnai1 subunit of GTP-binding protein
            catalyzes nucleotide exchange by selective perturbance of GDP-bound
            conformation of Gnai1 to metastable intermediate structural state
            and accelerates rate-limiting step of GDP release by surpassing
            kinetic barrier.
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   van Keulen,S.C. and Rothlisberger,U.
  TITLE     Effect of N-Terminal Myristoylation on the Active Conformation of
            Galphai1-GTP
  JOURNAL   Biochemistry 56 (1), 271-280 (2017)
   PUBMED   27936598
  REMARK    GeneRIF: Molecular dynamics simulation of a myristoylated G protein
            subunit alpha i1 (Galphai1)-GTP complex suggests that the
            myristoyl-induced conformational changes of the switch II and alpha
            helical domains create new possibilities for protein-protein
            interactions.
REFERENCE   3  (bases 1 to 4140)
  AUTHORS   Black,L.A., Thomas,C.J., Nix,G.N., Terwilliger,M.C., Sprang,S.R.
            and Ross,J.B.A.
  TITLE     Nanosecond Dynamics of Galphai1 Bound to Nucleotides or Ric-8A, a
            Galpha Chaperone with GEF Activity
  JOURNAL   Biophys J 111 (4), 722-731 (2016)
   PUBMED   27558716
  REMARK    GeneRIF: Induction of Galphai1 structural heterogeneity by Ric-8A
            provides a mechanism for nucleotide release
REFERENCE   4  (bases 1 to 4140)
  AUTHORS   Chiu,C.W.N., Monat,C., Robitaille,M., Lacomme,M., Daulat,A.M.,
            Macleod,G., McNeill,H., Cayouette,M. and Angers,S.
  TITLE     SAPCD2 Controls Spindle Orientation and Asymmetric Divisions by
            Negatively Regulating the Galphai-LGN-NuMA Ternary Complex
  JOURNAL   Dev Cell 36 (1), 50-62 (2016)
   PUBMED   26766442
REFERENCE   5  (bases 1 to 4140)
  AUTHORS   Simon,K., Hennen,S., Merten,N., Blattermann,S., Gillard,M.,
            Kostenis,E. and Gomeza,J.
  TITLE     The Orphan G Protein-coupled Receptor GPR17 Negatively Regulates
            Oligodendrocyte Differentiation via Galphai/o and Its Downstream
            Effector Molecules
  JOURNAL   J Biol Chem 291 (2), 705-718 (2016)
   PUBMED   26620557
  REMARK    GeneRIF: stimulation of GPR17 by the small molecule agonist
            MDL29,951 (2-carboxy-4,6-dichloro-1H-indole-3-propionic acid)
            decreases myelin basic protein expression levels mainly by
            triggering the Galphai/o signaling pathway.
REFERENCE   6  (bases 1 to 4140)
  AUTHORS   Berghuis,A.M., Lee,E., Raw,A.S., Gilman,A.G. and Sprang,S.R.
  TITLE     Structure of the GDP-Pi complex of Gly203-->Ala gialpha1: a mimic
            of the ternary product complex of galpha-catalyzed GTP hydrolysis
  JOURNAL   Structure 4 (11), 1277-1290 (1996)
   PUBMED   8939752
REFERENCE   7  (bases 1 to 4140)
  AUTHORS   Wall,M.A., Coleman,D.E., Lee,E., Iniguez-Lluhi,J.A., Posner,B.A.,
            Gilman,A.G. and Sprang,S.R.
  TITLE     The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma
            2
  JOURNAL   Cell 83 (6), 1047-1058 (1995)
   PUBMED   8521505
REFERENCE   8  (bases 1 to 4140)
  AUTHORS   Kleuss,C., Raw,A.S., Lee,E., Sprang,S.R. and Gilman,A.G.
  TITLE     Mechanism of GTP hydrolysis by G-protein alpha subunits
  JOURNAL   Proc Natl Acad Sci U S A 91 (21), 9828-9831 (1994)
   PUBMED   7937899
REFERENCE   9  (bases 1 to 4140)
  AUTHORS   Coleman,D.E., Berghuis,A.M., Lee,E., Linder,M.E., Gilman,A.G. and
            Sprang,S.R.
  TITLE     Structures of active conformations of Gi alpha 1 and the mechanism
            of GTP hydrolysis
  JOURNAL   Science 265 (5177), 1405-1412 (1994)
   PUBMED   8073283
REFERENCE   10 (bases 1 to 4140)
  AUTHORS   Jones,D.T. and Reed,R.R.
  TITLE     Molecular cloning of five GTP-binding protein cDNA species from rat
            olfactory neuroepithelium
  JOURNAL   J Biol Chem 262 (29), 14241-14249 (1987)
   PUBMED   2820999
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On or before Mar 12, 2021 this sequence version replaced
            XM_039107131.1, NM_013145.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643290.14885.1,
                                           SRR26643298.21414.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-337               JAXUCZ010000004.1  17706061-17706397
            338-380             JAXUCZ010000004.1  17758401-17758443
            381-522             JAXUCZ010000004.1  17758535-17758676
            523-680             JAXUCZ010000004.1  17769840-17769997
            681-809             JAXUCZ010000004.1  17771976-17772104
            810-939             JAXUCZ010000004.1  17780923-17781052
            940-1093            JAXUCZ010000004.1  17782584-17782737
            1094-4140           JAXUCZ010000004.1  17787130-17790176
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q11"
     gene            1..4140
                     /gene="Gnai1"
                     /gene_synonym="BPGTPB"
                     /note="G protein subunit alpha i1"
                     /db_xref="GeneID:25686"
                     /db_xref="RGD:2713"
     exon            1..337
                     /gene="Gnai1"
                     /gene_synonym="BPGTPB"
                     /inference="alignment:Splign:2.1.0"
     CDS             220..1284
                     /gene="Gnai1"
                     /gene_synonym="BPGTPB"
                     /note="guanine nucleotide binding protein, alpha
                     inhibiting 1; guanine nucleotide-binding protein G(i),
                     alpha-1 subunit; adenylate cyclase-inhibiting G alpha
                     protein; guanine nucleotide binding protein (G protein),
                     alpha inhibiting 1; guanine nucleotide binding protein (G
                     protein), alpha inhibiting activity polypeptide 1"
                     /codon_start=1
                     /product="guanine nucleotide-binding protein G(i) subunit
                     alpha-1"
                     /protein_id="NP_037277.1"
                     /db_xref="GeneID:25686"
                     /db_xref="RGD:2713"
                     /translation="MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGES
                     GKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDAARAD
                     DARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLD
                     RIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGV
                     TAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEE
                     KIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQF
                     VFDAVTDVIIKNNLKDCGLF"
     exon            338..380
                     /gene="Gnai1"
                     /gene_synonym="BPGTPB"
                     /inference="alignment:Splign:2.1.0"
     exon            381..522
                     /gene="Gnai1"
                     /gene_synonym="BPGTPB"
                     /inference="alignment:Splign:2.1.0"
     exon            523..680
                     /gene="Gnai1"
                     /gene_synonym="BPGTPB"
                     /inference="alignment:Splign:2.1.0"
     exon            681..809
                     /gene="Gnai1"
                     /gene_synonym="BPGTPB"
                     /inference="alignment:Splign:2.1.0"
     exon            810..939
                     /gene="Gnai1"
                     /gene_synonym="BPGTPB"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1093
                     /gene="Gnai1"
                     /gene_synonym="BPGTPB"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..4140
                     /gene="Gnai1"
                     /gene_synonym="BPGTPB"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcggcggcgg tagcggagcc tagcggtcgc cgggactcgc gggcgcggat cgtagagcgt
       61 cgcgggcggg gacggccgcc cgaggcggcg gcgtatcggt tagcagacct cgcctccagc
      121 ttgccggagc tgaggaccta gcggggccgc ctccgggcca gcctctcccc gctctgccag
      181 ccggccccgg cgccgagggc gcggcgacgc tcggccacca tgggctgcac actgagcgct
      241 gaggacaagg cggccgtgga gcgcagcaag atgatcgacc gcaacctccg ggaggacgga
      301 gagaaggcgg cgcgcgaggt caagctgctg ctgctgggtg ctggtgaatc cgggaagagc
      361 acaattgtga agcagatgaa aattatccac gaggctggct actcagagga agagtgtaag
      421 cagtacaaag cagtggtcta cagcaacacc atccagtcca tcattgccat cattagagcc
      481 atggggagat tgaaaatcga ctttggagac gctgctcgtg cggatgatgc tcgccaactc
      541 ttcgtgcttg ctggggctgc agaggaaggc tttatgaccg cggagctcgc cggcgtcata
      601 aagagactgt ggaaggacag cggtgtgcaa gcctgcttca acagatcccg ggagtaccag
      661 ctgaacgatt cggcggcgta ctacctgaat gacttggaca gaatagcaca accaaattac
      721 atcccaaccc agcaggatgt tctcagaact agagtgaaaa cgacgggaat tgtggaaacc
      781 cactttactt tcaaagatct tcattttaaa atgtttgacg tgggaggcca gagatcagag
      841 cggaagaagt ggattcactg ctttgaaggc gtgactgcca tcatcttctg tgtggccctg
      901 agtgactatg acctggttct tgctgaggat gaagaaatga accggatgca cgaaagcatg
      961 aagctgttcg atagcatatg taacaacaag tggtttacgg acacatccat catccttttc
     1021 ctgaacaaga aggacctctt cgaagagaag atcaaaaaga gtcccctcac gatatgctat
     1081 ccagaatatg caggctcaaa cacatatgaa gaggcggctg cgtatatcca gtgtcagttt
     1141 gaagacctca ataaaaggaa ggacacaaag gaaatttaca cccacttcac ttgcgccacg
     1201 gatacgaaga atgtgcagtt tgtgttcgat gctgtaacgg acgtcatcat aaagaataac
     1261 ctaaaagact gtggtctctt ctaagctctg cagtggggta gtaaaaatgc attttcaaac
     1321 cagacgagta cttatatatg gatctctcta gactagagtc tcccagcaac agagaatgta
     1381 gtgtacagca aatgcatcct ggacctaacc agagttctga ccaaagttgt tctgtatcgt
     1441 gtctttatat gaacgtaata gaaggacctt tcgagaaggc gaaagatggt cctggagtgt
     1501 gagagggagg gttgatgttg aagctggggc tctagtgtac tgatgatttc tgtgtaagtg
     1561 taaatatgca aatgtaagat gtatttataa cttctgattt cccacgttat gtttatgttg
     1621 taagagtggc aacttatggt cctagtgcga tggctttggg aaaacataac tgttaagtac
     1681 cttgtacgga atgacagatt ggttctgtgt ttggcagttt tgatcagctt tatttatgtt
     1741 cgtaccccta aattttaggc accataatta acattaggaa atgtcaaccc ccttaccctg
     1801 attctatgtt tacttatact cataaaatgt tatttgtata aacgttgcac agactatttt
     1861 agtaccatga tttgtcctct aaagtttctg tgtttcatgg ggatgatctt tcagaggttg
     1921 ctgcacctcc tttggatcag tcatttaagc attgagagca ttaggattta tttacagagt
     1981 gcatgacgtg ccgattctac tttacatttg ttttaaaaaa ctaaaattcc agatctttga
     2041 ggacacagga tctcagactt aaaaaccctt ctgtcttaaa ttcactgctt tgaactggat
     2101 cttagtatct ggatggtatt tctgcattgc cttggagtga tgccaaattc attttataca
     2161 caataaccac tgttttattt ataatgacgt ttaggtgagt gatgagagca tttttgtgag
     2221 taaaagattt ttagtattat gtgaattttg agacattgat ttgtgtattt tttttttttg
     2281 acaaagccct tgtatttgag cacacatgat acttccttgt ctaggagaag accagaaaac
     2341 atgatattgc tgatattata caggcttttg attcataggg aactagtttg aggttcatta
     2401 ctttatgtca aaaatgactt cttttgcatt aggtattcga attccttcca cttgctcgga
     2461 tttctactct tttaaagcta cagtatgatt tttgacatta agtggacaat tattgttaga
     2521 aattaaataa tagtatttag caagatcagt gagtgtatgt gggcttaaat aggtgttacg
     2581 taacagctac atactgaact ttgttagtgc catttttaaa gaatcattta cggtgtgtgc
     2641 gtgcccttgt ttcagcaagc tgaatagcat tccccagctc cttccccttt ttaactcatc
     2701 tacaccattg agtctttcca taagctttgc tggtgcgtag ttgctcactt gagttagaag
     2761 ttgctggttc cttagagtct ccctgctgac gtttgtgcat atgtaagtgt atggagaacc
     2821 gtaaactaca tccacgcctg tttacttttg ggcacatttg ttctcttctt tgtaatgtaa
     2881 agaaattcag agttacccaa attccttcag tgttagtcta gattattttc tgtgcctttc
     2941 aggaaagatc catttccatt cattgactaa tggaagacct gtcagaccca ccttgtcaag
     3001 ctgtgtatga aattcaagta tactctggat acatttgagt gatgaaaaat agggtttgaa
     3061 agccacaaag aagcacatat tggtgaccac cattggtgaa tccctgaact ctgtgtaact
     3121 gtgctactaa tacaccccaa taaatttaaa tttttaaaat tttacaaacc tgttgactga
     3181 attcattttg tattataata taggcgtagc atgtaaaaac tcccttcttt gtttccagta
     3241 ctctctcatt ttagctttat ctgtctgaga agctccagtt tgccctgagt gttcttatag
     3301 agtatgattt caaatttaaa tgtcccgcct gtcatcccct atgggaattc atctcacagc
     3361 tcactgtacc catagaatca caaagaattg gggtcctagg tcacaacaat atgcattaga
     3421 agaggcttgt ggtggtctgc cccttcttgc tttctttaat gcttggctga ggcaatgagt
     3481 agattcattt gtgccctttt gcttccagca tgatgccttg ccacagagct aaagcagtgg
     3541 aatcagttga tcagactgga atattcaaat gcataagtca agataagtga cctcagatgt
     3601 attttgatag tgacagaaag ctgtataaca tacctactga ataccctctt tgttggggaa
     3661 atacaagcta taggtcacat gtgacctaaa gcatctgtga acgtggttca acacaaaacc
     3721 attaatgtaa aacattataa gtctctgtgt gtgtgtgtgt gtctgtgtgt gtctgtgtgt
     3781 ctgtatgtct gtgagtgtgt gaaagacaga cagctggccc cccagggggg atgaaatttc
     3841 ctaaatcttt cagttaatat tggccacctg gacttcctgt gtctccattt ctagatggag
     3901 agttcatttt cattaaataa cctctagttt agtttggggt aggaggggta tttttgtttg
     3961 tttcacattc tcaggtcatt attgagcttc cccttaaaat ggcttgtgaa taaaattcca
     4021 tcaaccccat gtaacctagg ttggcctgtt taatacaaaa tccagactac atcaccctct
     4081 gttttaactc acactggctc tgagttgtca ataaaattga gtattattta aagcctccca
//
