LOCUS       NM_001033931            2947 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus germ cell-less 1, spermatogenesis associated
            (Gmcl1), transcript variant 1, mRNA.
ACCESSION   NM_001033931 XM_579332
VERSION     NM_001033931.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Yachie,N., Petsalaki,E., Mellor,J.C., Weile,J., Jacob,Y., Verby,M.,
            Ozturk,S.B., Li,S., Cote,A.G., Mosca,R., Knapp,J.J., Ko,M., Yu,A.,
            Gebbia,M., Sahni,N., Yi,S., Tyagi,T., Sheykhkarimli,D., Roth,J.F.,
            Wong,C., Musa,L., Snider,J., Liu,Y.C., Yu,H., Braun,P.,
            Stagljar,I., Hao,T., Calderwood,M.A., Pelletier,L., Aloy,P.,
            Hill,D.E., Vidal,M. and Roth,F.P.
  TITLE     Pooled-matrix protein interaction screens using Barcode Fusion
            Genetics
  JOURNAL   Mol Syst Biol 12 (4), 863 (2016)
   PUBMED   27107012
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Maekawa,M., Ito,C., Toyama,Y., Suzuki-Toyota,F., Kimura,T.,
            Nakano,T. and Toshimori,K.
  TITLE     Stage-specific expression of mouse germ cell-less-1 (mGCL-1), and
            multiple deformations during mgcl-1 deficient spermatogenesis
            leading to reduced fertility
  JOURNAL   Arch Histol Cytol 67 (4), 335-347 (2004)
   PUBMED   15700541
REFERENCE   3  (bases 1 to 2947)
  AUTHORS   Masuhara,M., Nagao,K., Nishikawa,M., Kimura,T. and Nakano,T.
  TITLE     Enhanced degradation of MDM2 by a nuclear envelope component, mouse
            germ cell-less
  JOURNAL   Biochem Biophys Res Commun 308 (4), 927-932 (2003)
   PUBMED   12927808
REFERENCE   4  (bases 1 to 2947)
  AUTHORS   Kleiman,S.E., Yogev,L., Gal-Yam,E.N., Hauser,R., Gamzu,R.,
            Botchan,A., Paz,G., Yavetz,H., Maymon,B.B., Schreiber,L.,
            Barzilai,S., Amariglio,N., Rechavi,G. and Simon,A.J.
  TITLE     Reduced human germ cell-less (HGCL) expression in azoospermic men
            with severe germinal cell impairment
  JOURNAL   J Androl 24 (5), 670-675 (2003)
   PUBMED   12954656
REFERENCE   5  (bases 1 to 2947)
  AUTHORS   Kimura,T., Ito,C., Watanabe,S., Takahashi,T., Ikawa,M.,
            Yomogida,K., Fujita,Y., Ikeuchi,M., Asada,N., Matsumiya,K.,
            Okuyama,A., Okabe,M., Toshimori,K. and Nakano,T.
  TITLE     Mouse germ cell-less as an essential component for nuclear
            integrity
  JOURNAL   Mol Cell Biol 23 (4), 1304-1315 (2003)
   PUBMED   12556490
REFERENCE   6  (bases 1 to 2947)
  AUTHORS   Nili,E., Cojocaru,G.S., Kalma,Y., Ginsberg,D., Copeland,N.G.,
            Gilbert,D.J., Jenkins,N.A., Berger,R., Shaklai,S., Amariglio,N.,
            Brok-Simoni,F., Simon,A.J. and Rechavi,G.
  TITLE     Nuclear membrane protein LAP2beta mediates transcriptional
            repression alone and together with its binding partner GCL
            (germ-cell-less)
  JOURNAL   J Cell Sci 114 (Pt 18), 3297-3307 (2001)
   PUBMED   11591818
REFERENCE   7  (bases 1 to 2947)
  AUTHORS   Leatherman,J.L., Kaestner,K.H. and Jongens,T.A.
  TITLE     Identification of a mouse germ cell-less homologue with conserved
            activity in Drosophila
  JOURNAL   Mech Dev 92 (2), 145-153 (2000)
   PUBMED   10727854
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from JAXUCZ010000004.1, FM064983.1
            and BP492225.1.
            
            On Apr 28, 2015 this sequence version replaced NM_001033931.1.
            
            Summary: This gene encodes a nuclear envelope protein that contains
            a broad-complex, tramtrack and bric a brac/ Pox virus and Zinc
            finger (BTB/POZ) protein-protein interaction domain. In mouse, this
            gene is expressed at high levels in the testis, and null mutant
            mice exhibit morphologically abnormal sperm that have chromatin
            condensation defects. These defects result in significantly
            impaired fertility, demonstrating a role for this gene in male germ
            cell development in mouse. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Mar 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY862307.1, SRR26643289.13455.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-464               JAXUCZ010000004.1  120736794-120737257 c
            465-588             JAXUCZ010000004.1  120729316-120729439 c
            589-685             JAXUCZ010000004.1  120725810-120725906 c
            686-742             JAXUCZ010000004.1  120724696-120724752 c
            743-1239            FM064983.1         1-497
            1240-1567           BP492225.1         334-661
            1568-1655           JAXUCZ010000004.1  120703879-120703966 c
            1656-2946           JAXUCZ010000004.1  120697976-120699266 c
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q34"
     gene            1..2947
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /note="germ cell-less 1, spermatogenesis associated"
                     /db_xref="GeneID:312516"
                     /db_xref="RGD:1359265"
     exon            1..464
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     CDS             211..1785
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /note="isoform 1 is encoded by transcript variant 1; germ
                     cell-less homolog 1; germ cell-less protein-like 1; germ
                     cell-less, spermatogenesis associated 1"
                     /codon_start=1
                     /product="germ cell-less protein-like 1 isoform 1"
                     /protein_id="NP_001029103.1"
                     /db_xref="GeneID:312516"
                     /db_xref="RGD:1359265"
                     /translation="MGALSSRVLRPAGRTEQPEPTPGAGGSARRSDAGEDAAHGFCYC
                     PGGRKRKRSSGTFCYCHPESETDEDEDEGDEQQRLLNTPRRKKLKSTSKYIYQTLFLN
                     GENSDIKICALGEEWSLHKIYLCQSGYFSSMFSGSWKESSMNIIELEIPDQNIDIEAL
                     QVAFGSLYRDDVLIKPSRVVAILAAACMLQLDGLIQQCGETMKETISVRTVCGYYTSA
                     GTYGLDSVKKKCLEWLLNNLMTHQSVELFKELSINVMRQLIGSSNLFVMQVEMDVYTA
                     LKKWMFLQLVPSWNGSLKQLLTETDVWFSKWKKDFEGTTFLETEQGKPFAPVFRHLRL
                     QYIISDLASARIIEQDSLVPSEWLASVYKQQWLAMLRAEQDSEVGPQEINKEELEGNS
                     MRCGRKLAKDGEYCWRWTGFNFGFDLLVTYTNRYIIFKRNTLNQPCSGSVSLQPRRSI
                     AFRLRLASFDSSGKLICSRATGYQILTLEKDQEQVVMNLDSRLLIFPLYICCNFLYIS
                     PEKRTESNRHPENPGH"
     exon            465..588
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            589..685
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            686..783
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            784..896
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            897..962
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1047
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1138
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1276
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1346
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1422
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1423..1568
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1656
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..2947
                     /gene="Gmcl1"
                     /gene_synonym="Gcl"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcctgcgccc tgcggcgcgc gctttgttgg gagaacgagg gggcgaggtc ttgagaagcc
       61 ggtcggctaa ggcgcgacgg gcggggctga ggatggaggt gactgcgttc gcggcgacgg
      121 ttggggctgc gcgcgaggcg gcggcagagt aggcggtgga gcgacaggta tggcgtggcg
      181 gcccctcagt gctccctagg cgccggagcc atgggcgctc tgagcagccg ggtcctgcgg
      241 cccgcggggc gcacagagca gcccgaaccc acgcccggtg ctgggggctc ggcccgcaga
      301 tcggacgcgg gcgaagatgc ggcccacggc ttctgttact gtcccggcgg ccgcaagcgc
      361 aagcgcagca gcggcacttt ctgctactgt caccccgagt cagagacaga cgaagacgag
      421 gacgagggcg acgagcagca gaggctgctg aacacgccgc gcaggaaaaa attaaagagc
      481 acatcgaaat acatctacca aacgctgttt ttgaatggtg aaaacagtga cattaagatc
      541 tgtgctctag gtgaagagtg gagcttacac aaaatctact tatgtcaatc tggctacttt
      601 tctagtatgt tcagtggttc ttggaaagaa tccagcatga atattattga actggagatt
      661 cctgaccaga acattgatat agaagcactg caggttgcat ttggatcact gtatcgagat
      721 gacgtcttaa taaagcccag cagggttgtc gccattttgg cagcagcttg catgctgcag
      781 ttggatggtt tgatacagca gtgtggtgag accatgaagg agaccatctc tgtaagaact
      841 gtgtgtggct attacacatc ggcagggacc tatggactag actctgtaaa gaaaaagtgc
      901 ctcgagtggc tgctgaacaa cctcatgact caccagagtg tggagctttt caaagaactc
      961 agtataaacg tcatgaggca gctcattggt tcatctaact tatttgtgat gcaagtggaa
     1021 atggatgtat atacagctct gaaaaagtgg atgttccttc agttggtgcc ttcctggaat
     1081 gggtctttaa agcagctttt gacagaaaca gatgtctggt tttcaaagtg gaaaaaagac
     1141 tttgaaggga cgactttcct tgaaactgag cagggaaaac catttgcgcc cgtgttcaga
     1201 catttaaggc tacagtacat tatcagtgat ctggcttctg caaggatcat tgagcaggac
     1261 tctctggtac cttcagaatg gctggcttca gtgtataagc agcagtggtt ggctatgctt
     1321 cgggctgaac aagacagtga agtggggcct caagaaatca ataaagaaga acttgaggga
     1381 aatagcatga ggtgtggtag aaagcttgcc aaagatggtg aatactgctg gcggtggaca
     1441 ggttttaatt ttggctttga cctccttgtg acttacacca atcgatacat cattttcaaa
     1501 cgcaatacgc taaaccagcc atgtagtgga tctgtcagct tgcagcctcg aagaagcata
     1561 gcatttagat tgcgcttggc ttctttcgac agtagtggaa aactcatatg cagtagagcg
     1621 actggctacc aaatcctgac gcttgagaaa gaccaggagc aagtggtgat gaacttggac
     1681 agcagactgc tgatcttccc tctatacatc tgctgtaact tcttgtatat atcaccagaa
     1741 aaaagaactg agagtaatcg tcacccagaa aacccaggac actgaggcac gcatcggtgg
     1801 ccagctttaa cttaatgacc tactgcgttc acgtccaagg tgactaacag tgaccggcct
     1861 tatgaactgt gtggacgatg cagatgtcct caccctcatt acatttctat gcacatatga
     1921 aaaagtatgt taaaaccaag aaagcatctg tcaaaccata tatgttaaaa ggatatctat
     1981 cagctctagc tttcatttag tagcagtaga aaattgctgt aggtaaattt ctcacttctt
     2041 tgcaacaaaa tatagaattg attttgtgct tgaattttga tcctatctaa tgttagtgaa
     2101 cttattcatc tgtaaatgtg ttcctgtttt gttaagagaa tgctaaggat gtagtttgag
     2161 tggccaatcg taaatgctct ttcaatcggt gcctttaaag ctgtcctttc atttttcagt
     2221 ctcccggtta agtcctctgt ttagtttaac cctttccagt ctctgcttct ttttaaccat
     2281 tatttccttt ttcatggttt gggagactaa gcttaggaat tggttttgtt ttgtttcgtt
     2341 ttgttttttt attctaaatg tatgcatttt catatttttt aaactttaac tctcctacag
     2401 ttttaaatga caacaagagt cagctttagg acttgaaata aacttgaata agggaaatca
     2461 gtttgattct gaggatattc cttcgcctta cgtgcgtggt cttgtctggc attccgtgta
     2521 ctcttacgtt atgaattatt tatgtacaga aacatcttta aacattttca tattctctga
     2581 tcgttggaat ttatgttcaa attttagttg ggaatcttgt ttcctactta agctcaggac
     2641 tttataacct ctgaactgag tgcctttgag tcacatacgc aaatagattt catggtgaat
     2701 gtacaggagg agctgggaac aaagcattca tgttagaaca acgtactttg tcaaggataa
     2761 gatgtatacg catcatgact tgtatgtgtt ggttccttgt gtttattgct attgaaaata
     2821 gccttgatca ttgtcctcca gtcagagtaa gtgcttttgt ggttgatgta tgtttttagt
     2881 atttttttaa attggaatca tttttatgta tctgttcaaa taataaattg ctcatttgga
     2941 aaaggaa
//
