LOCUS       NM_001270786            2552 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus glutaminase 2 (Gls2), transcript variant 1, mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_001270786
VERSION     NM_001270786.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Zhou,X., Zhuo,M., Zhang,Y., Shi,E., Ma,X. and Li,H.
  TITLE     miR-190a-5p regulates cardiomyocytes response to ferroptosis via
            directly targeting GLS2
  JOURNAL   Biochem Biophys Res Commun 566, 9-15 (2021)
   PUBMED   34111670
  REMARK    GeneRIF: miR-190a-5p regulates cardiomyocytes response to
            ferroptosis via directly targeting GLS2.
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Cardona,C., Sanchez-Mejias,E., Davila,J.C., Martin-Rufian,M.,
            Campos-Sandoval,J.A., Vitorica,J., Alonso,F.J., Mates,J.M.,
            Segura,J.A., Norenberg,M.D., Rama Rao,K.V., Jayakumar,A.R.,
            Gutierrez,A. and Marquez,J.
  TITLE     Expression of Gls and Gls2 glutaminase isoforms in astrocytes
  JOURNAL   Glia 63 (3), 365-382 (2015)
   PUBMED   25297978
  REMARK    GeneRIF: GABAergic neurons and astrocytes express Gls and Gls2
            isoenzymes in nucleus and mitochondria, in addition to
            glutamatergic neurons
REFERENCE   3  (bases 1 to 2552)
  AUTHORS   Savilov,P.N. and Yakovlev,V.N.
  TITLE     Phosphate-dependent glutaminase response to liver injury and
            hyperbaric oxygenation
  JOURNAL   Bull Exp Biol Med 157 (3), 299-301 (2014)
   PUBMED   25065305
  REMARK    GeneRIF: In animals with chronic hepatitis; hyperbaric oxygenation
            restricted the stimulating effect of hepatic resection on
            phosphate-dependent glutaminase activity.
REFERENCE   4  (bases 1 to 2552)
  AUTHORS   Hasson,S.A., Kane,L.A., Yamano,K., Huang,C.H., Sliter,D.A.,
            Buehler,E., Wang,C., Heman-Ackah,S.M., Hessa,T., Guha,R.,
            Martin,S.E. and Youle,R.J.
  TITLE     High-content genome-wide RNAi screens identify regulators of parkin
            upstream of mitophagy
  JOURNAL   Nature 504 (7479), 291-295 (2013)
   PUBMED   24270810
REFERENCE   5  (bases 1 to 2552)
  AUTHORS   Martin-Rufian,M., Tosina,M., Campos-Sandoval,J.A., Manzanares,E.,
            Lobo,C., Segura,J.A., Alonso,F.J., Mates,J.M. and Marquez,J.
  TITLE     Mammalian glutaminase Gls2 gene encodes two functional alternative
            transcripts by a surrogate promoter usage mechanism
  JOURNAL   PLoS One 7 (6), e38380 (2012)
   PUBMED   22679499
  REMARK    GeneRIF: Study demonstrated expression of alternative transcripts
            of the mammalian Gls2 gene. Transcriptional mechanisms giving rise
            to GLS2 variants and isolation of novel GLS2 transcripts in human,
            rat and mouse are presented.
REFERENCE   6  (bases 1 to 2552)
  AUTHORS   Suzuki,S., Tanaka,T., Poyurovsky,M.V., Nagano,H., Mayama,T.,
            Ohkubo,S., Lokshin,M., Hosokawa,H., Nakayama,T., Suzuki,Y.,
            Sugano,S., Sato,E., Nagao,T., Yokote,K., Tatsuno,I. and Prives,C.
  TITLE     Phosphate-activated glutaminase (GLS2), a p53-inducible regulator
            of glutamine metabolism and reactive oxygen species
  JOURNAL   Proc Natl Acad Sci U S A 107 (16), 7461-7466 (2010)
   PUBMED   20351271
REFERENCE   7  (bases 1 to 2552)
  AUTHORS   Chung-Bok,M.I., Vincent,N., Jhala,U. and Watford,M.
  TITLE     Rat hepatic glutaminase: identification of the full coding sequence
            and characterization of a functional promoter
  JOURNAL   Biochem J 324 (Pt 1) (Pt 1), 193-200 (1997)
   PUBMED   9164856
REFERENCE   8  (bases 1 to 2552)
  AUTHORS   Smith,E.M. and Watford,M.
  TITLE     Molecular cloning of a cDNA for rat hepatic glutaminase. Sequence
            similarity to kidney-type glutaminase
  JOURNAL   J Biol Chem 265 (18), 10631-10636 (1990)
   PUBMED   2191954
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1 and BC089776.1.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089776.1, SRR26643286.21266.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-418               JAXUCZ010000007.1  1201879-1202296
            419-2552            BC089776.1         258-2391
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..2552
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /note="glutaminase 2"
                     /db_xref="GeneID:192268"
                     /db_xref="RGD:620359"
     exon            1..418
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     CDS             237..2045
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /EC_number="3.5.1.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     glutaminase 2 (liver, mitochondrial); GLS; L-glutaminase;
                     L-glutamine amidohydrolase; liver mitochondrial
                     glutaminase"
                     /codon_start=1
                     /product="glutaminase liver isoform, mitochondrial isoform
                     1"
                     /protein_id="NP_001257715.1"
                     /db_xref="GeneID:192268"
                     /db_xref="RGD:620359"
                     /translation="MRSMRALQNALSRAGSHGQRGGWGHPSRGPLLGGGVRYYFGEAA
                     AQGRGTPHSHQPQHSDHDASNSGMLPRLGDLLFYTIAEGQERIPIHKFTTALKATGLQ
                     TSDPRLQDCMSKMQRMVQESSSGGLLDRELFQKCVSSNIVLLTQAFRKKFVIPDFEEF
                     TGHVDRIFEDAKELTGGKVAAYIPHLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQ
                     SCVKPLTYAISVSTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSS
                     LIKMDCNKAEKFDFVLQYLNKMAGNEFMGFSNATFQSEKETGDRNYAIGYYLKEKKCF
                     PKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSL
                     MHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGISF
                     CQKLVSLFNFHNYDNLRHCARKLDPRREGGEVRNKTVVNLLFAAYSGDVSALRRFALS
                     AVDMEQKDYDSRTALHVAAAEGHIDVVKFLIEACKVNPFVKDRWGNIPLDDAVQFNHL
                     EVVKLLQDYHDSYMLSETQAEVAAETLSKENLESMV"
     exon            419..518
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            519..640
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            641..770
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            771..850
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            851..1014
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1073
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1106
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1165
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1232
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1283
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1460
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1461..1592
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1685
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..1747
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1748..1824
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1888
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..2528
                     /gene="Gls2"
                     /gene_synonym="Ga"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtgttagagg ctgtgaaggg gctatgagtg cacctgcggg gtcgaagtcg ccccggactg
       61 gcccctcccc ggactccaac ccctcccacc cggccggccc tccccactgc gggttccgct
      121 ggccggggga ctgttcccgg ggctcctagt ctcggccgcc actcttccgc ccctccctcc
      181 tcgccgccgc tcgcacgtct gcagctcccg ggccgagcgg gcggcgcgct gggggcatgc
      241 gctccatgag ggctctgcag aacgcgctga gccgcgcggg cagccacggc cagaggggag
      301 gctggggtca cccgagccgg ggcccgctcc tgggcggggg tgtccggtac tacttcggtg
      361 aggccgcggc gcagggcagg gggacgccgc acagccacca gccgcagcac tcggaccatg
      421 atgcctcgaa cagtggcatg ttgcctcgac ttggtgacct gcttttctac accattgcag
      481 aggggcagga acgcatcccc atccataagt tcaccacggc cctgaaggcc actggactgc
      541 agacgtcaga cccacggctc caggactgca tgagcaagat gcagcgcatg gtccaagagt
      601 ccagcagtgg tggcctcttg gaccgagaac tcttccaaaa gtgtgtgagc agcaacattg
      661 tgctcctgac tcaggcattc cggaagaagt ttgtcattcc tgactttgag gagttcacag
      721 gccatgtgga tcgtatattt gaggatgcca aagagctcac tggaggcaaa gtggcagcct
      781 acatccctca cctggccaag tcaaacccag atctttgggg cgtctccctg tgcactgtgg
      841 atgggcagcg acactctgtg ggccacacga agatcccttt ctgcctacag tcctgtgtca
      901 agccactcac ttacgccatc tccgtgagca ccttaggcac cgactacgtg cacaagttcg
      961 tgggcaagga acccagtggt ctgcgctata acaaactctc cctcaacgag gaaggaatcc
     1021 ctcataaccc catggtcaat gctggtgcta ttgtggtcag ctccctgatc aagatggact
     1081 gtaacaaagc agagaagttt gactttgtgt tacagtatct gaacaagatg gctgggaatg
     1141 aattcatggg gttcagcaat gccacattcc agtcagagaa ggagactggg gatcggaatt
     1201 acgccattgg ctattacctc aaggagaaga agtgcttccc taagggagtg gacatgatgg
     1261 ctgcccttga tctctatttc cagctgtgct ctgtggaggt tacctgtgag tcaggcagtg
     1321 tcatggcggc cactcttgcc aatggcggca tctgccccat cacaggggag agcgtgctga
     1381 gtgcggaagc cgtgcgcaac accctcagcc tcatgcactc ctgtggcatg tatgacttct
     1441 cgggccagtt tgccttccac gtgggcctgc cagccaagtc agctgtgtcg ggagccatcc
     1501 tcctggttgt acccaatgtc atggggatga tgtgtctgtc acccccgtta gacaagctgg
     1561 ggaacagcca caggggcatc agcttctgcc agaagttggt gtctctgttt aacttccaca
     1621 actacgacaa cctgcggcac tgtgctcgga agttagaccc acggagggaa gggggggaag
     1681 ttcggaacaa gaccgtggtg aacctgttat ttgctgcata tagtggagat gtctcagctc
     1741 ttcgaaggtt tgccttgtct gccgtggata tggagcagaa ggactatgat tcccgcacag
     1801 ccctacatgt ggcggcagcg gaaggacaca ttgacgttgt caagtttctg atcgaggctt
     1861 gcaaagtgaa tccttttgtc aaggacaggt ggggcaacat tcccctggat gatgccgtgc
     1921 agttcaatca cctggaggtg gtcaaactgc ttcaggatta ccatgactcc tacatgctgt
     1981 ctgagactca agctgaggta gcagctgaga ctctgtcaaa agagaactta gagagcatgg
     2041 tgtgagcaca ggccaggggc agcccctgct caagaaaaag catgagccgg ccacacattt
     2101 aacccaaggc caccaaaaat actattgcaa gctgcttcag tggggatcaa cacagccatc
     2161 tggtgacaca ggccagtgtt ttctgtgaga atcaaaatgc cccattccct catcggacag
     2221 cacagagaaa agcttcagtg gacacctgag cagagctagc cacggagacc tcaaggtata
     2281 gcttaagtga catcctccac cagaaagtag cccaggcttt tacccaggtc cccatttcaa
     2341 cttccttgga gagcctctag ctacatgcat atgtatctgt cacagagcaa gagaggtggg
     2401 tgagagccca atcacctggc tttagaaatc tgcagagatc tgtccatctt agccaagaca
     2461 tgctgctact gctgacagga gttttataga caaagtattt tgtgttcaaa taaactttaa
     2521 ttacatgaaa aaaaaaaaaa aaaaaaaaaa aa
//
