LOCUS       NM_053724               7378 bp    mRNA    linear   ROD 09-JUL-2024
DEFINITION  Rattus norvegicus glycine receptor, alpha 3 (Glra3), mRNA.
ACCESSION   NM_053724 XM_344515
VERSION     NM_053724.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Velazquez-Flores,M.A., Sanchez-Chavez,G., Morales-Lazaro,S.L., Ruiz
            Esparza-Garrido,R., Canizales-Ontiveros,A. and Salceda,R.
  TITLE     Streptozotocin-Induced Diabetic Rats Showed a Differential Glycine
            Receptor Expression in the Spinal Cord: A GlyR Role in Diabetic
            Neuropathy
  JOURNAL   Neurochem Res 49 (3), 684-691 (2024)
   PUBMED   38017313
  REMARK    GeneRIF: Streptozotocin-Induced Diabetic Rats Showed a Differential
            Glycine Receptor Expression in the Spinal Cord: A GlyR Role in
            Diabetic Neuropathy.
REFERENCE   2  (bases 1 to 7378)
  AUTHORS   Raltschev,C., Hetsch,F., Winkelmann,A., Meier,J.C. and Semtner,M.
  TITLE     Electrophysiological Signature of Homomeric and Heteromeric Glycine
            Receptor Channels
  JOURNAL   J Biol Chem 291 (34), 18030-18040 (2016)
   PUBMED   27382060
  REMARK    GeneRIF: data show that the current-voltage relationships of
            homomeric channels formed by the alpha2 or alpha3 subunits change
            upon receptor desensitization from a linear to an inwardly
            rectifying shape, in contrast to their heteromeric counterparts.
REFERENCE   3  (bases 1 to 7378)
  AUTHORS   Kono,Y. and Hulsmann,S.
  TITLE     Presynaptic facilitation of glycinergic mIPSC is reduced in mice
            lacking alpha3 glycine receptor subunits
  JOURNAL   Neuroscience 320, 1-7 (2016)
   PUBMED   26851771
REFERENCE   4  (bases 1 to 7378)
  AUTHORS   Huang,X., Chen,H., Michelsen,K., Schneider,S. and Shaffer,P.L.
  TITLE     Crystal structure of human glycine receptor-alpha3 bound to
            antagonist strychnine
  JOURNAL   Nature 526 (7572), 277-280 (2015)
   PUBMED   26416729
REFERENCE   5  (bases 1 to 7378)
  AUTHORS   McCracken,L.M., Trudell,J.R., McCracken,M.L. and Harris,R.A.
  TITLE     Zinc-dependent modulation of alpha2- and alpha3-glycine receptor
            subunits by ethanol
  JOURNAL   Alcohol Clin Exp Res 37 (12), 2002-2010 (2013)
   PUBMED   23895467
REFERENCE   6  (bases 1 to 7378)
  AUTHORS   Eichler,S.A., Forstera,B., Smolinsky,B., Juttner,R., Lehmann,T.N.,
            Fahling,M., Schwarz,G., Legendre,P. and Meier,J.C.
  TITLE     Splice-specific roles of glycine receptor alpha3 in the hippocampus
  JOURNAL   Eur J Neurosci 30 (6), 1077-1091 (2009)
   PUBMED   19723286
  REMARK    GeneRIF: the long (L) splice variant of GlyR alpha3 predominates in
            the brain of rodents. In primary hippocampal neurons GlyR alpha3L
            clusters also preferred glutamatergic nerve endings while alpha3K
            was mainly in a diffuse state.
REFERENCE   7  (bases 1 to 7378)
  AUTHORS   Chen,X., Webb,T.I. and Lynch,J.W.
  TITLE     The M4 transmembrane segment contributes to agonist efficacy
            differences between alpha1 and alpha3 glycine receptors
  JOURNAL   Mol Membr Biol 26 (5), 321-332 (2009)
   PUBMED   19626554
  REMARK    GeneRIF: Using chimeras of alpha1 and alpha3 subunit containing
            glycine receptors, we identified the structurally divergent M4
            transmembrane segment and C-terminal tail as a specific determinant
            of the efficacy difference.
REFERENCE   8  (bases 1 to 7378)
  AUTHORS   Meier,J.C., Henneberger,C., Melnick,I., Racca,C., Harvey,R.J.,
            Heinemann,U., Schmieden,V. and Grantyn,R.
  TITLE     RNA editing produces glycine receptor alpha3(P185L), resulting in
            high agonist potency
  JOURNAL   Nat Neurosci 8 (6), 736-744 (2005)
   PUBMED   15895087
  REMARK    GeneRIF: GlyR alpha3(P185L), a result of cytidine 554 deamination,
            confers high glycine sensitivity (EC50 approximately 5 microM) to
            neurons and thereby promotes the generation of sustained chloride
            conductances associated with tonic inhibition
REFERENCE   9  (bases 1 to 7378)
  AUTHORS   Nikolic,Z., Laube,B., Weber,R.G., Lichter,P., Kioschis,P.,
            Poustka,A., Mulhardt,C. and Becker,C.M.
  TITLE     The human glycine receptor subunit alpha3. Glra3 gene structure,
            chromosomal localization, and functional characterization of
            alternative transcripts
  JOURNAL   J Biol Chem 273 (31), 19708-19714 (1998)
   PUBMED   9677400
REFERENCE   10 (bases 1 to 7378)
  AUTHORS   Kuhse,J., Schmieden,V. and Betz,H.
  TITLE     Identification and functional expression of a novel ligand binding
            subunit of the inhibitory glycine receptor
  JOURNAL   J Biol Chem 265 (36), 22317-22320 (1990)
   PUBMED   2176214
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000016.1.
            
            On Aug 31, 2022 this sequence version replaced NM_053724.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M55250.1, SRR26360194.1138999.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN14984014, SAMN14984017
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-560               JAXUCZ010000016.1  39856447-39857006   c
            561-688             JAXUCZ010000016.1  39816987-39817114   c
            689-756             JAXUCZ010000016.1  39796646-39796713   c
            757-980             JAXUCZ010000016.1  39766242-39766465   c
            981-1063            JAXUCZ010000016.1  39748230-39748312   c
            1064-1201           JAXUCZ010000016.1  39256852-39256989   c
            1202-1416           JAXUCZ010000016.1  39254173-39254387   c
            1417-1560           JAXUCZ010000016.1  39231805-39231948   c
            1561-1605           JAXUCZ010000016.1  39230243-39230287   c
            1606-7378           JAXUCZ010000016.1  39210536-39216308   c
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="16"
                     /map="16p11"
     gene            1..7378
                     /gene="Glra3"
                     /note="glycine receptor, alpha 3"
                     /db_xref="GeneID:114516"
                     /db_xref="RGD:621229"
     exon            1..560
                     /gene="Glra3"
                     /inference="alignment:Splign:2.1.0"
     CDS             490..1884
                     /gene="Glra3"
                     /note="glycine receptor subunit alpha-3; glycine receptor,
                     alpha 3 subunit; glycine receptor subunit alpha-3-like"
                     /codon_start=1
                     /product="glycine receptor subunit alpha-3 precursor"
                     /protein_id="NP_446176.4"
                     /db_xref="GeneID:114516"
                     /db_xref="RGD:621229"
                     /translation="MAHVRHFRTLLSGFYFWEAALLLSLVATKETNSARSRSAPMSPS
                     DFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSIAETTMDYRVNIFLRQKWN
                     DPRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGANFHEVTTDNKLLRIFKNGNVL
                     YSIRLTLTLSCPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQDEAPVQVAEGLTLP
                     QFLLKEEKDLRYCTKHYNTGKFTCIEVRFHLERQMGYYLIQMYIPSLLIVILSWVSFW
                     INMDAAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEY
                     AAVNFVSRQHKELLRFRRKRKNKTEAFALEKFYRFSDTDDEVRESRFSFTAYGMGPCL
                     QAKDGVVPKGPNHAVQVMPKSADEMRKVFIDRAKKIDTISRACFPLAFLIFNIFYWVI
                     YKILRHEDIHHQQD"
     exon            561..688
                     /gene="Glra3"
                     /inference="alignment:Splign:2.1.0"
     exon            689..756
                     /gene="Glra3"
                     /inference="alignment:Splign:2.1.0"
     exon            757..980
                     /gene="Glra3"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1063
                     /gene="Glra3"
                     /inference="alignment:Splign:2.1.0"
     exon            1064..1201
                     /gene="Glra3"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1416
                     /gene="Glra3"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1560
                     /gene="Glra3"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1605
                     /gene="Glra3"
                     /inference="alignment:Splign:2.1.0"
     exon            1606..7378
                     /gene="Glra3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccgcgctcc gctcggtggg gttttcgcaa aggtggagag cgagactctg gctcttggac
       61 ctggtggagc agcattctca attttgactg ctgttgtgct tcctcgatcc ctgcctgggc
      121 cagagccgag aaaaggcgag tccttttctg ctgatagaac tgtgaggaac tgttgctcag
      181 gacctctgca ctcctgacac gtcgtcacag tctctcgctt ttctgtggct tcacagtgaa
      241 atcactcgca cgtttgtagc cgggtgaatc cttctgctcc agtgctcaga ggaggggtct
      301 aggaaaacat caagtgtttg aaagagggat tcggagtact gcgggagctg tctgaggtgc
      361 tgaagcttcc caagcgtttg cctctagcag ccccagaagg ggttagatgg cacgttttca
      421 cttctgtctt tctggtcccc catgccttgg ataagactgt tttcaggatc gggaatatct
      481 ttccgtatca tggcccacgt gagacacttt cggacactac tttctggatt ttacttctgg
      541 gaagccgcac tgttactgag tttggtggcc acgaaggaaa caaacagtgc acggtcccga
      601 agtgccccaa tgtcaccttc tgattttctg gacaaactaa tggggaggac atccgggtat
      661 gatgcaagaa tcaggcccaa cttcaaaggt cctccagtta atgtcacatg caacatattc
      721 ataaacagct ttggctccat cgcagagacg actatggatt acagagtaaa cattttcctt
      781 cgtcagaagt ggaatgatcc tcgccttgca tacagtgaat accctgatga ttcattagac
      841 ctcgacccat ccatgttgga ctccatatgg aaacctgact tgttctttgc taatgagaag
      901 ggggctaact tccacgaagt caccaccgat aacaagctgc taagaatttt caaaaatgga
      961 aatgttcttt attcaataag gctgacatta acactctctt gtccaatgga tctcaagaat
     1021 ttcccaatgg atgttcaaac atgcataatg caactcgaaa gctttgggta cacgatgaat
     1081 gatctcattt tcgaatggca agatgaagca ccagtacaag tggctgaagg actcactttg
     1141 cctcaatttc tgttgaaaga agaaaaagat ttgcgatact gcactaaaca ctacaataca
     1201 ggaaagttta catgcataga agtacgattt catcttgagc ggcaaatggg ctactacttg
     1261 atccagatgt acattcccag ccttctgatt gtcattctgt cctgggtctc attctggatt
     1321 aacatggatg cagctccggc tcgggtagcg ttgggtatca ccactgtact tacgatgacc
     1381 acgcagagtt ctggatcccg ggcttcttta ccaaaggtgt cctatgtcaa ggcaattgac
     1441 atttggatgg cagtgtgtct cctttttgtg ttctcagcac ttctggagta tgcagccgtg
     1501 aattttgtat caaggcaaca caaagaactg ctgaggtttc ggcgaaagag gaaaaataaa
     1561 acagaagctt ttgcactgga gaagttttac cgtttctcag acacggatga tgaggtgagg
     1621 gagagtcggt tcagcttcac tgcctatgga atggggccct gtcttcaagc aaaggatggt
     1681 gtggttccaa aaggtcccaa ccatgctgtc caggtcatgc caaagagcgc cgatgaaatg
     1741 aggaaggtct tcatcgacag ggctaagaag atcgacacca tctcccgagc ctgctttccg
     1801 ctagcatttc tgatttttaa cattttctac tgggttatct ataaaatcct taggcatgaa
     1861 gacattcatc atcagcaaga ttaagtctat gaaggcatac aaaaacagag cattgagaag
     1921 aaagagtccc cgctaaagga gcggggtgtg tgtgcatgtg gggtgcaggt gagagtcagt
     1981 gcatcagaac agcatatggg gaaagaattt attttgattt cctatgtaaa atagtgagac
     2041 agtttggtta aatgatacaa aatgtacatc gtgggtgtcc tgcttcataa tcctgacagg
     2101 tgatctgttg tcttcctagt gtgaatatgt gttgcatatg ctttacaagc acatagagta
     2161 gacatgataa ctacattggg aggtcttgag ttttaattca attatagtaa acactgcgga
     2221 agcaagattt acacaataaa atgtataaag caagtatagc aaaatgatga gtataataaa
     2281 atgtcttgag atatacagca tatcgatgaa gtaacaagat cattaaatgt caaggtcact
     2341 catgtcagaa cactgtccat agaaacagtg tggaaccaac aaccacaata aaaacaaaat
     2401 cggatggtca aaatgaaagc catcatttag aacactatgg cttaagcaat catgtttata
     2461 aaattctact tatctttaca tctctaggaa tgaggaggaa gatgttgaag acagtttttt
     2521 cttgtaaata agtgttgcct ttattttaat tagtgctaaa cactaatgac taagccaata
     2581 catttgtttt ccttaaatta agtaggggct tgtttgtatg tatggtgttt ttaatgtaac
     2641 taaataagat ccaataagac ttttctatct agtgtagaat gagactttaa atattaatta
     2701 tactaaagca cttacatttt gataaaaaac aaatacaaat agattcataa aagattttta
     2761 ataagaatca tttctctcac tgaagtttca tctcatcagt gtaatctatc atcccaaaat
     2821 gcctccaaat aacaggagaa tgggttttaa ttctaagtga tttaaacata ttagctccat
     2881 cgagtgagat tattttaatc tttatgagct aatgcattca gagtagtagg gactcgtgag
     2941 ttgtaaagaa tttaaaagca actctgcttg aaagttgttc atgacccatg caacatttca
     3001 gctgtattga agttttgtta gctcagtcac acagcagcta ccaatctctg attcttttac
     3061 tgtttttctt aattattgtt gtcattaata ttatacttta aaacctttgg agtgacttta
     3121 agatacttta attattggct agtctataaa attatataca gtgaaaatat caagaatttt
     3181 aaaacaactc agatatgtta tagaaaaatc aagtttttga acccaatggt actatttttt
     3241 tgaccttcaa caactccgaa aactcaaagc agaaactgcc atttaaagcc aaaaagaagc
     3301 ttttcaatat ctctgtagtt ttattagcca accatagatt ttaaaactaa ctttttaaaa
     3361 agtaatccat gctgatcagt gtttgctaat aaaaagtaaa aatatcactt ttataagtag
     3421 tttacacaag ataagaacat tgtggttgtg attttatgca aagtctacaa ggaaaataat
     3481 ttgataccaa gcgttccctc tgcttgaaaa ctatccactt gaaaatcaga tatacatgtt
     3541 atcctttcct gtttatgagt gaaaaccata gaaatatatt catgtaatat atatcagagc
     3601 aagatatgtt acttaggctg agagttagcc agagcgtttg aagtaatgat gaattaaccc
     3661 cttccatgta tgctagcaca catttttcta ttcttaatgt atcttacttc ataagttttc
     3721 taacatcact taaaatgagt aaaccctgcc aaaagcaaag agaaatataa taatcacaaa
     3781 cacatatatg ccccagaggt gtgatcatca ctccccaagt gtgatgagct ttcatatttg
     3841 aggttgctag atgtcatttt tctttttttt taaatctctg aagtagaaaa ataatgtatc
     3901 agtcatgaga aaatttgtca agaatgacat tcattctaca gaaatattgc tatctcaaaa
     3961 gtatatttcc ttattttttc ttatgaataa ttctgctttc tattatgttt attctgtatg
     4021 ccaacacaca cacacacaca cacacacaca cacacacaca caacccacta aaaacaactt
     4081 tctcttacaa ggcagccatt acaaagcaaa cagggatgaa ttaaaggagc ttaaaaattt
     4141 gtccccttta ataaactaat taatgcaata ttaccgaacc tttaaaatta tagtaacaac
     4201 aacatcctgt ggtattagta tcaaaatcct atatcaaatg aagattttga tccatagtac
     4261 tgagaattgg aaccagagtt tgttacatgt gagggaagag attattgcta aaccatagcc
     4321 cgagtcccaa actagaatta aaactagagc aatagcaatg agaagaaata attcaaatat
     4381 ttacctaacc acaactcatt taagctgagc tccagtgcaa acgttctgag gtattctaaa
     4441 ttagccatac tgcaactatg gggagtgggg cgcagggtgt ggagtggagc acatgcatga
     4501 catttatttc acttaatttt aaaatctatt cattttaaca tgaaatattt ggttccccag
     4561 gacacacatt tttaggtaat tcattatctt attagtatag atgtgtataa ttttcttttt
     4621 cttccctaat cactacaaga cctagaactt tctttatgac ttacaggagt tatcagaact
     4681 actttagaca tgaaacagag ttttaagcca tcctttttct ctccagaatt tcttccatta
     4741 catttatcca aagatctaat cagctctcag ctctgctaca cagagtatca tggacagtta
     4801 ccatatttca caatacattc ttctatgcat aaattctaat aaatttaatt tttaactttg
     4861 aactataagc acatacctat tatataattg catgcattga ttttgtgaat gtcctgtatc
     4921 tatataagat actggaagtt atcaagaaaa ttatgaaaaa taaatcatat atttaaaaga
     4981 agataatgag ctcttaacta cactaatatt tttgtaaatg aacacactat ttgaatatag
     5041 tcaggtgaat ctttcatatt gtttcaaaaa attacattca tctcattttg tgaaaatagt
     5101 tttctattct cagcatgata tattttaatc tgctagttac ttggaagaat ggtgtatcat
     5161 attggcaagt aaatttcctt ttattataat gtgtaccaca tgaagttgac ttgtgagaag
     5221 atgacaggta acaagctaaa ataggaaata tattttagta ttaaatattg gaaaatggaa
     5281 gcaaaacgat ccaatcccct ctgaaataga ttttatatta tcatatactg tttaatatta
     5341 gcagcatata ttcttttatt tttcagccaa attggtcagt attcattgaa actgaacagg
     5401 tcaaaactat gagagtagac attatccttg acatattagg aacacattag aatggtttcc
     5461 aaccttgaag caatttaatt tgacaatcag tccaagccaa ttataatcaa tgaaaactga
     5521 aacctataaa cataggaagt gtcttatctc tgtgagcaga ttattatatt agtgattgaa
     5581 attgtaagac tttcttaaat aatacctacc aaccaagcac aactataata cttgcattcc
     5641 tggcctgcta atttgtattc attttcagca agatgagccg gattgtgttg cctatgattg
     5701 actttaagga cactgtaaag tcgaaataaa tggctgactt ttgtgccttt tgggaaagaa
     5761 tttagtgatc aaactgcaca atattttaaa tatcaaattt caagtcctat aaaactggac
     5821 cgaagaattg tctaacctgt gccaataggt ctaaagaact taccagaggc aatctttttc
     5881 aaataatttt aacaagaata ccttagctga atttatgtga ataaatagac agtggaaaca
     5941 aaacatgaaa cttctattca agatttacat gatctcataa tgcctccaga actcattact
     6001 gcattttctt tgggggatta gagtcctaga cataatacta catagtaacc acagggaggt
     6061 ggtttaatgg ggagagtgct ttcttggtat ttgaaagacc ctaggttcca tgcaaacaat
     6121 actaacaata ataagactta gacagactcc tagctcccat ctagagactg aagtcagcag
     6181 tctcttcctt tgcagcataa tttgcataag aaaaggttaa ctgtcatcca aaaccattta
     6241 cttcctaaaa aggaaactcc actctgtctt gaccaaagcc cactctgcat ttcctaatac
     6301 ctgctgccat tgtttttgtc cgactcaata tccatattat tcattttatt tatttaagaa
     6361 attcattgca gcactttgtc aaatcataag atatattcag gtttgcaaaa tagtgaaata
     6421 taaaattgta tatttagtaa aagttattgg tttttatgaa gttctatgtc attattttca
     6481 gtaactttaa attctgtcct ataacttagt acttatttta aattaaatat ttagtcaatg
     6541 tatttcagat ataaatatat agaaagtctt agaatgatag aaaatgtaaa acaaactatg
     6601 aaataaacac cgatgctttt atttagatta aaggtataat tcaaatatat tgacctgatt
     6661 aagtttattt taatacttta tttttctgtg aagtgcatga gcaaaaatta tcttcataga
     6721 caaagcattt ttacacagtc tctagccaag cttgttaatg gtcttgagga ataggaactc
     6781 tgaattaaat gccaaaaaag gattatgatg taaaactgta cttgaccttg tttgaataat
     6841 tttgcatttc tttactgttt catctttatg aattatgcag aagctgctct gtaagttcac
     6901 gttttagtgt atgatgagtg ataaaacttt gaaaccatgg agtgaagcat cttgacattt
     6961 tatcagcatt gtggtacaca aaatctaaag aaacaatgtc atatactctt ccatggattc
     7021 cttttacatg ttgtgtaatg acttcattta ctaatgagaa aagatgttct aaaaggctat
     7081 tcctcatttt atgatactag ttattttctg cacattgaaa gtacatcttc ctgaggacgg
     7141 tatttgagtc tccctttctg tctagatgga tgccacagtt tttcacgaca ttgtgatttg
     7201 gaggtatgaa gatgttttgc atggctttac caacttgttt ttgttatatg acctatagta
     7261 taattttgaa gctaacaaaa tctctaatga atcattaacc tgtgaacagt atattgtctt
     7321 cctcgtggaa ctgcagataa tttgatcaga aatataataa aaattaatgt ttttcaag
//
