LOCUS       NM_001393806            1748 bp    mRNA    linear   ROD 28-MAR-2024
DEFINITION  Rattus norvegicus gap junction protein, beta 6 (Gjb6), transcript
            variant 2, mRNA.
ACCESSION   NM_001393806 XM_006252079
VERSION     NM_001393806.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Jothi,J., Janardhanam,V.A. and Rama,K.
  TITLE     Connexin 30 mediated rewiring of glucose metabolism in rat C6
            xenograft and grades of glioma
  JOURNAL   Mol Cell Biochem 470 (1-2), 157-164 (2020)
   PUBMED   32462383
  REMARK    GeneRIF: Connexin 30 mediated rewiring of glucose metabolism in rat
            C6 xenograft and grades of glioma.
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Arun,S., Ravisankar,S. and Vanisree,A.J.
  TITLE     Implication of connexin30 on the stemness of glioma: connexin30
            reverses the malignant phenotype of glioma by modulating IGF-1R,
            CD133 and cMyc
  JOURNAL   J Neurooncol 135 (3), 473-485 (2017)
   PUBMED   28875331
  REMARK    GeneRIF: Investigation of stemness-related CD133 and cMyc in human
            samples and rat xenografts exhibited a reciprocal relationship
            between Cx30 and IGF-1R in the low and high grades (HG) of glioma.
            Cx30 was completely abolished in HG; levels of IGF-1R, CD133 and
            cMyc expression were positively correlated with HG. Cx30
            transfection could attenuate the malignant burden of glioma in rat
            xenografts.
            Erratum:[J Neurooncol. 2021 May;152(3):621-622. PMID: 33761056]
REFERENCE   3  (bases 1 to 1748)
  AUTHORS   Arun,S., Vanisree,A.J. and Ravisankar,S.
  TITLE     Connexin 30 downregulates Insulin-like growth factor receptor-1,
            abolishes Erk and potentiates effects of an IGF-R inhibitor in a
            glioma cell line
  JOURNAL   Brain Res 1643, 80-90 (2016)
   PUBMED   27130897
  REMARK    GeneRIF: Study demonstrates that connexin 30 has the potential to
            alter the Insulin-like growth factor-1 receptor mediated pathway
            thereby influencing the growth, proliferation and migration of
            glioma cells.
REFERENCE   4  (bases 1 to 1748)
  AUTHORS   Kamiya,K., Yum,S.W., Kurebayashi,N., Muraki,M., Ogawa,K.,
            Karasawa,K., Miwa,A., Guo,X., Gotoh,S., Sugitani,Y., Yamanaka,H.,
            Ito-Kawashima,S., Iizuka,T., Sakurai,T., Noda,T., Minowa,O. and
            Ikeda,K.
  TITLE     Assembly of the cochlear gap junction macromolecular complex
            requires connexin 26
  JOURNAL   J Clin Invest 124 (4), 1598-1607 (2014)
   PUBMED   24590285
REFERENCE   5  (bases 1 to 1748)
  AUTHORS   Allard,C., Carneiro,L., Grall,S., Cline,B.H., Fioramonti,X.,
            Chretien,C., Baba-Aissa,F., Giaume,C., Penicaud,L. and Leloup,C.
  TITLE     Hypothalamic astroglial connexins are required for brain glucose
            sensing-induced insulin secretion
  JOURNAL   J Cereb Blood Flow Metab 34 (2), 339-346 (2014)
   PUBMED   24301293
  REMARK    GeneRIF: These results illustrate that astroglial connexins 30 and
            43 contribute to hypothalamic GS.
REFERENCE   6  (bases 1 to 1748)
  AUTHORS   Princen,F., Robe,P., Gros,D., Jarry-Guichard,T., Gielen,J.,
            Merville,M.P. and Bours,V.
  TITLE     Rat gap junction connexin-30 inhibits proliferation of glioma cell
            lines
  JOURNAL   Carcinogenesis 22 (3), 507-513 (2001)
   PUBMED   11238193
REFERENCE   7  (bases 1 to 1748)
  AUTHORS   Rash,J.E., Yasumura,T., Davidson,K.G., Furman,C.S., Dudek,F.E. and
            Nagy,J.I.
  TITLE     Identification of cells expressing Cx43, Cx30, Cx26, Cx32 and Cx36
            in gap junctions of rat brain and spinal cord
  JOURNAL   Cell Commun Adhes 8 (4-6), 315-320 (2001)
   PUBMED   12064610
REFERENCE   8  (bases 1 to 1748)
  AUTHORS   Lamartine,J., Munhoz Essenfelder,G., Kibar,Z., Lanneluc,I.,
            Callouet,E., Laoudj,D., Lemaitre,G., Hand,C., Hayflick,S.J.,
            Zonana,J., Antonarakis,S., Radhakrishna,U., Kelsell,D.P.,
            Christianson,A.L., Pitaval,A., Der Kaloustian,V., Fraser,C.,
            Blanchet-Bardon,C., Rouleau,G.A. and Waksman,G.
  TITLE     Mutations in GJB6 cause hidrotic ectodermal dysplasia
  JOURNAL   Nat Genet 26 (2), 142-144 (2000)
   PUBMED   11017065
REFERENCE   9  (bases 1 to 1748)
  AUTHORS   Nagy,J.I., Patel,D., Ochalski,P.A. and Stelmack,G.L.
  TITLE     Connexin30 in rodent, cat and human brain: selective expression in
            gray matter astrocytes, co-localization with connexin43 at gap
            junctions and late developmental appearance
  JOURNAL   Neuroscience 88 (2), 447-468 (1999)
   PUBMED   10197766
REFERENCE   10 (bases 1 to 1748)
  AUTHORS   Lautermann,J., Frank,H.G., Jahnke,K., Traub,O. and Winterhager,E.
  TITLE     Developmental expression patterns of connexin26 and -30 in the rat
            cochlea
  JOURNAL   Dev Genet 25 (4), 306-311 (1999)
   PUBMED   10570462
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On Mar 15, 2021 this sequence version replaced XM_006252079.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC078753.1, CV080813.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760389, SAMEA5760393
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-111               JAXUCZ010000015.1  35410028-35410138   c
            112-279             JAXUCZ010000015.1  35407641-35407808   c
            280-1748            JAXUCZ010000015.1  35400147-35401615   c
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..1748
                     /gene="Gjb6"
                     /gene_synonym="Cx30; Cxnf"
                     /note="gap junction protein, beta 6"
                     /db_xref="GeneID:84403"
                     /db_xref="RGD:621830"
     exon            1..111
                     /gene="Gjb6"
                     /gene_synonym="Cx30; Cxnf"
                     /inference="alignment:Splign:2.1.0"
     exon            112..279
                     /gene="Gjb6"
                     /gene_synonym="Cx30; Cxnf"
                     /inference="alignment:Splign:2.1.0"
     exon            280..1748
                     /gene="Gjb6"
                     /gene_synonym="Cx30; Cxnf"
                     /inference="alignment:Splign:2.1.0"
     CDS             294..1079
                     /gene="Gjb6"
                     /gene_synonym="Cx30; Cxnf"
                     /note="gap junction protein, beta 6 (connexin 30); gap
                     junction membrane channel protein beta 6; connexin f"
                     /codon_start=1
                     /product="gap junction beta-6 protein"
                     /protein_id="NP_001380735.1"
                     /db_xref="GeneID:84403"
                     /db_xref="RGD:621830"
                     /translation="MDWGTLHTVIGGVNKHSTSIGKVWITVIFIFRVMILVVAAQEVW
                     GDEQEDFVCNTLQPGCKNVCYDHFFPVSHIRLWALQLIFVSTPALLVAMHVAYYRHET
                     ARKFIRGEKRNEFKDLEDIKRQKVRIEGSLWWTYTSSIFFRIIFEAAFMYVFYFLYNG
                     YHLPWVLKCGIDPCPNLVDCFISRPTEKTVFTVFMISASVICMLLNVAELCYLLLKLC
                     FRRSKRTQAQRNHPNHALKESKQNEMNELISDSGQNAITSFPS"
ORIGIN      
        1 tctgttcccg attctgttcc cgccgcgaac cgccggcttc tgatccaacc gacgctcagg
       61 gcaggagctc tgagtgccgc tgcaagcttg gagccggctc ccaccgagcg ggtgacattc
      121 ccactgccac cctaaacaga gcagcagggc agggccacca ccactaacac tgctgactgc
      181 cagaggagta gaagggaaag ccagaagtcc ttgaggaggg attttgcagt ggttcttgga
      241 ctggacgacg cactggaagt ccttgggaac gagactcaga aggagcgtgc gcaatggact
      301 gggggaccct gcacactgtc atcggcggcg tgaacaagca ctcgaccagc atagggaagg
      361 tatggatcac ggtcatcttc atcttccgag tcatgatcct cgtggtggcc gcccaggaag
      421 tgtggggaga tgagcaggag gacttcgtct gcaacacgct gcagccgggg tgcaagaacg
      481 tctgctacga ccacttcttc cccgtgtccc acattcggct ctgggccctg cagctgatct
      541 ttgtgtccac cccagccctg ctggtggcca tgcacgtggc ctactacaga catgaaactg
      601 cccgaaagtt tatccgcggg gagaagagga acgagtttaa agacctggag gacatcaaac
      661 ggcagaaggt gcgcatcgag ggctcgctgt ggtggacgta caccagcagc atcttcttcc
      721 gcatcatctt cgaagccgcc ttcatgtatg tgttctactt cctctacaat gggtaccacc
      781 tgccctgggt gctgaaatgc ggcattgacc cctgccccaa tctcgtggac tgcttcattt
      841 ccaggccaac cgagaaaacg gtgttcactg tttttatgat ttccgcatct gtgatttgca
      901 tgctgctcaa tgtggccgag ttgtgttacc tgctgcttaa gctgtgtttt aggagatcca
      961 agagaacaca ggcccagaga aaccacccca accatgccct gaaagagagc aagcagaatg
     1021 aaatgaatga gctgatctcg gatagcggtc agaatgccat caccagcttc ccaagttaag
     1081 catttcaagg tcaagcgtag gtgagccctt ctgagtgcgc tccctctttg taggcatcga
     1141 tctgacaact tctctaggcc aagatgagtc acagcaaata gatacctgag ttcaattatt
     1201 gtaggacacc gattgtgatc cagctctgaa aaccaagtcc tcatgacaac tgggccttga
     1261 agtcacctgg acaagtccta tgtgaactgt tgtgacgtag tatttcccga aaatttatat
     1321 aacttgttta taaagagcaa tttatctaga aattgatagc tttatcagta agatattttt
     1381 ataggaatga atattttagt tctgactttg aatttatata aagtattttt ttaatgaccg
     1441 gccttcttta cctggaaaaa ataaacaaag ttagctttac aagagcaccc ccaagtatct
     1501 aaagtttgaa cttgtaaagg gtctagcatt ttataaacat tgtcttcaca ctgtctgagg
     1561 acggtttggt gagctagtgt gtgtgcctgg gatgggacat ggtcattgga caacatacat
     1621 cgccgtttgc taagtatgaa tgggatgcaa ggaagtgaga ccctctctaa aattagcctt
     1681 tccaccggtg gcgaacagtg taagcacaaa tacaatcact ccaataaagc ttgaccctgc
     1741 tgcttcag
//
