LOCUS       NM_001004099            2457 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus gap junction protein, beta 2 (Gjb2), mRNA.
ACCESSION   NM_001004099 XM_237945
VERSION     NM_001004099.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Moscato,S., Cabiati,M., Bianchi,F., Panetta,D., Burchielli,S.,
            Massimetti,G., Del Ry,S. and Mattii,L.
  TITLE     Heart and liver connexin expression related to the first stage of
            aging: A study on naturally aged animals
  JOURNAL   Acta Histochem 122 (8), 151651 (2020)
   PUBMED   33171391
  REMARK    GeneRIF: Heart and liver connexin expression related to the first
            stage of aging: A study on naturally aged animals.
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Lin,J., Huang,H., Lv,G., Xu,X., Lin,W., Xu,X., Cheng,J. and
            Zheng,M.
  TITLE     Chronic prenatal hypoxia impairs cochlear development, a mechanism
            involving connexin26 expression and promoter methylation
  JOURNAL   Int J Mol Med 41 (2), 852-858 (2018)
   PUBMED   29207085
  REMARK    GeneRIF: These results demonstrate that chronic prenatal hypoxia
            caused hearing impairment, and suggest that promoter region
            hypermethylation and expression downregulation of Cx26 underlie the
            mechanism of action
REFERENCE   3  (bases 1 to 2457)
  AUTHORS   Santolim,L.V., Amaral,M.E.C.D., Fachi,J.L., Mendes,M.F. and
            Oliveira,C.A.
  TITLE     Vitamin E and caloric restriction promote hepatic homeostasis
            through expression of connexin 26, N-cad, E-cad and cholesterol
            metabolism genes
  JOURNAL   J Nutr Biochem 39, 86-92 (2017)
   PUBMED   27816814
  REMARK    GeneRIF: Supplementation with vitamin E, with or without caloric
            restriction, upregulated the mRNA expression of Cx26, N-cad, E-cad
            and genes involved in cholesterol metabolism, suggesting the
            maintenance of hepatic homeostasis.
REFERENCE   4  (bases 1 to 2457)
  AUTHORS   Pinto,B.I., Garcia,I.E., Pupo,A., Retamal,M.A., Martinez,A.D.,
            Latorre,R. and Gonzalez,C.
  TITLE     Charged Residues at the First Transmembrane Region Contribute to
            the Voltage Dependence of the Slow Gate of Connexins
  JOURNAL   J Biol Chem 291 (30), 15740-15752 (2016)
   PUBMED   27143357
  REMARK    GeneRIF: the role of the charged residues at the end of TM-1 in
            voltage sensing in Cx26, Cx46, and Cx50.
REFERENCE   5  (bases 1 to 2457)
  AUTHORS   Adam,C. and Cyr,D.G.
  TITLE     Role of Specificity Protein-1 and Activating Protein-2
            Transcription Factors in the Regulation of the Gap Junction Protein
            Beta-2 Gene in the Epididymis of the Rat
  JOURNAL   Biol Reprod 94 (6), 120 (2016)
   PUBMED   27053364
  REMARK    GeneRIF: SP1 and TFAP2A regulate Gjb2 promoter activity during
            epididymal differentiation in rat.
REFERENCE   6  (bases 1 to 2457)
  AUTHORS   Bennett,B.C., Purdy,M.D., Baker,K.A., Acharya,C., McIntire,W.E.,
            Stevens,R.C., Zhang,Q., Harris,A.L., Abagyan,R. and Yeager,M.
  TITLE     An electrostatic mechanism for Ca(2+)-mediated regulation of gap
            junction channels
  JOURNAL   Nat Commun 7, 8770 (2016)
   PUBMED   26753910
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2457)
  AUTHORS   Sakamoto,H., Oyamada,M., Enomoto,K. and Mori,M.
  TITLE     Differential changes in expression of gap junction proteins
            connexin 26 and 32 during hepatocarcinogenesis in rats
  JOURNAL   Jpn J Cancer Res 83 (11), 1210-1215 (1992)
   PUBMED   1336494
REFERENCE   8  (bases 1 to 2457)
  AUTHORS   Winterhager,E., Stutenkemper,R., Traub,O., Beyer,E. and Willecke,K.
  TITLE     Expression of different connexin genes in rat uterus during
            decidualization and at term
  JOURNAL   Eur J Cell Biol 55 (1), 133-142 (1991)
   PUBMED   1655436
REFERENCE   9  (bases 1 to 2457)
  AUTHORS   Zhang,J.T. and Nicholson,B.J.
  TITLE     Sequence and tissue distribution of a second protein of hepatic gap
            junctions, Cx26, as deduced from its cDNA
  JOURNAL   J Cell Biol 109 (6 Pt 2), 3391-3401 (1989)
   PUBMED   2557354
REFERENCE   10 (bases 1 to 2457)
  AUTHORS   Nicholson,B., Dermietzel,R., Teplow,D., Traub,O., Willecke,K. and
            Revel,J.P.
  TITLE     Two homologous protein components of hepatic gap junctions
  JOURNAL   Nature 329 (6141), 732-734 (1987)
   PUBMED   2823143
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1 and BC078952.1.
            
            On Sep 24, 2016 this sequence version replaced NM_001004099.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC078952.1, SRR26643287.31524.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN12840116, SAMN12840127
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-12                JAXUCZ010000015.1  35381942-35381953   c
            13-2457             BC078952.1         1-2445
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..2457
                     /gene="Gjb2"
                     /gene_synonym="Cx26; CXN-26; Cxne"
                     /note="gap junction protein, beta 2"
                     /db_xref="GeneID:394266"
                     /db_xref="RGD:728891"
     exon            1..267
                     /gene="Gjb2"
                     /gene_synonym="Cx26; CXN-26; Cxne"
                     /inference="alignment:Splign:2.1.0"
     exon            268..2433
                     /gene="Gjb2"
                     /gene_synonym="Cx26; CXN-26; Cxne"
                     /inference="alignment:Splign:2.1.0"
     CDS             290..970
                     /gene="Gjb2"
                     /gene_synonym="Cx26; CXN-26; Cxne"
                     /note="gap junction channel protein connexin 26; gap
                     junction membrane channel protein beta 2; connexin e"
                     /codon_start=1
                     /product="gap junction beta-2 protein"
                     /protein_id="NP_001004099.1"
                     /db_xref="GeneID:394266"
                     /db_xref="RGD:728891"
                     /translation="MDWGTLQSILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVW
                     GDEQADFVCNTLQPGCKNVCYDHYFPISHIRLWALQLIMVSTPALLVAMHVAYRRHEK
                     KRKFMKGEIKNEFKDIEEIKTQKVRIEGSLWWTYTTSIFFRVIFEAVFMYVFYIMYNG
                     FFMQRLVKCNAWPCPNTVDCFISRPTEKTVFTVFMISVSGICILLNITELCYLFIRYC
                     SGKSKRPV"
ORIGIN      
        1 aagatcgcag cgcgcggcgc gctccgctct cccaactcgc agccagtcgg cggcgctccc
       61 gcctactgag cgcagcctcc accaggatcc gcggggacca gctcgggatc agccggcgac
      121 ccacttctga ccaacccagg agcggcccga tacccactcc cgaccaaccc gcgaccgacc
      181 cagggaccca ctccggacct gctccttaca ggggacagcg cctcgccgct tcccgccgcc
      241 cagcgccgca cgctcctcgg gacacagtgc caaccatcca gaggacaaga tggattgggg
      301 cacactacag agcatcctcg ggggtgtcaa caagcactcc accagcattg ggaaaatctg
      361 gctcactgtc ctcttcatct tccgcatcat gatcctcgtg gtggccgcga aggaggtgtg
      421 gggagatgag caagccgatt ttgtttgcaa cactctccag cctggctgta agaatgtgtg
      481 ctacgaccac tacttcccca tctctcacat ccggctctgg gctctgcagc tgatcatggt
      541 gtccacgccg gccctcctgg tagctatgca cgtggcctac cggagacacg aaaagaaacg
      601 gaagttcatg aagggagaga taaagaacga gtttaaggac atcgaagaga tcaaaaccca
      661 gaaggtccgt atcgaagggt ccctgtggtg gacctacacc accagcatct tcttccgggt
      721 catcttcgaa gctgtcttca tgtatgtctt ttacatcatg tacaatggct tcttcatgca
      781 gcgtctggtg aagtgtaacg cctggccttg tcccaataca gtggactgct tcatttccag
      841 gcccacagaa aagactgtct tcacggtgtt catgatctct gtgtctggaa tttgcatcct
      901 gctaaacatc acagagctgt gctatctgtt cattaggtat tgctcaggga agtccaaaag
      961 accagtctaa tgcattgcct ggctgttaag caaagatgag ggagaggatg aggcaacctg
     1021 tgcttagtta tcagagttca gctaccagca tctcccgggc aaacattccc accttaaatg
     1081 ccgccatttg aagtcccccg caggcctccc atgaaactcc agaagcctcc atgggcctcc
     1141 cttcccccaa agctcccaaa caaaggccca attctatgcc tgtattaatg ggttctaaag
     1201 ttagttagac cccgtgctgg tgtgactatg ctttaggata cattcacagt ttaaacaaag
     1261 ggatctcaca ttgtttctct tcctctgagg acaggagaca tgagcccagt cctgaggaag
     1321 gtacagagaa agttccttct tccgggtccc cttccccaag ttgcccccag ttaagggtaa
     1381 agaatcttcg ttctgttatt ttctttcata gtttaagttt gcaacaatgg acaaaagcta
     1441 tttaatgttt caagctagct gtgtcctttt ttttttttta aatgaaaacc ttaaaatgat
     1501 aggttctttt gttcttaaaa tgatctggaa agcattatac attcctccta tttcagaggt
     1561 tcggtttgtg atgtgagcat ggtgtataac cagatctcac aaggtcttta aaacgttggc
     1621 cttttggtta tgggaaacct gggctgtggc tgagagccca cctactgtat tcatccttgg
     1681 tgtgctgagt acagcccgca acaacgttac agcctgtctc aaatgagaca aactggaagc
     1741 ttctcgtgtt agcttctgac aagaagaggc cttgattaaa attttcaacc gtaattttgt
     1801 gtaagaggca gataggttat gcctacaact gccccctgcc atgagcctaa ctcagccccc
     1861 ctccaccccc agctcgtcta ctctgtagct gtgggatgtg gcagtcagta tcaaaagact
     1921 tcatgagttt gcttgggaat ttcactgcca tggtacaatt taatggtgca gaaacaagat
     1981 ggggtggttt tcaaagaacc gatgaaactt ctagactcta aatcctgttg attaaaactg
     2041 agtttttcta ctttgaatgt ctgtttgcct cccttttcag cattgccttc taaactggaa
     2101 acagaaatgt tgatatttgg aaaaaataga agaaactagt ttaggtcaat gtgtaacttt
     2161 tctaggacaa gttgaacctt agcattgtca ttctgcctga tgtgttgtcc acaagatgac
     2221 agtcaacaaa tccaacaggg gacacttctt cctgccaaga atgtcgttgg gaagccattc
     2281 tgtaacaata aataagagtt gtggtttcat gcttgtcact gatataattt tgtaaactcc
     2341 taaatgtcat ttattaaaat atgattattt tgtatacttt aaaattaaat aaggttttta
     2401 atagaatacc aaactttaat gatcaatagt aaaaaaaaaa aaaaaaaaaa aaaaaaa
//
