LOCUS       NM_021654               1617 bp    mRNA    linear   ROD 16-NOV-2024
DEFINITION  Rattus norvegicus gap junction protein, alpha 4 (Gja4), mRNA.
ACCESSION   NM_021654
VERSION     NM_021654.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Taylor,S.Z., Jacobsen,N.L., Pontifex,T.K., Langlais,P. and
            Burt,J.M.
  TITLE     Serine 319 phosphorylation is necessary and sufficient to induce a
            Cx37 conformation that leads to arrested cell cycling
  JOURNAL   J Cell Sci 133 (12) (2020)
   PUBMED   32350069
  REMARK    GeneRIF: Serine 319 phosphorylation is necessary and sufficient to
            induce a Cx37 conformation that leads to arrested cell cycling.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Guo,S., Yang,Y., Yang,Z., You,H., Shi,Y., Hu,Z., Meng,Z. and
            Xiao,J.
  TITLE     Improving myocardial fractional flow reserve in coronary
            atherosclerosis via CX37 gene silence: a preclinical validation
            study in pigs
  JOURNAL   Interact Cardiovasc Thorac Surg 26 (1), 139-145 (2018)
   PUBMED   29049831
  REMARK    GeneRIF: FFR levels increased along with decreased doses of siRNA
            CX37 lentivirus, indicating that siRNA CX37 lentivirus may reduce
            the risk of coronary atherosclerosis and provide a potential
            approach to treat coronary heart disease.
REFERENCE   3  (bases 1 to 1617)
  AUTHORS   Jacobsen,N.L., Pontifex,T.K., Li,H., Solan,J.L., Lampe,P.D.,
            Sorgen,P.L. and Burt,J.M.
  TITLE     Regulation of Cx37 channel and growth-suppressive properties by
            phosphorylation
  JOURNAL   J Cell Sci 130 (19), 3308-3321 (2017)
   PUBMED   28818996
  REMARK    GeneRIF: Substituting alanine at serine residues 275, 302 and 328
            eliminated Cx37-induced cell death, supported proliferation and
            reduced the GJCh closed state probability.
REFERENCE   4  (bases 1 to 1617)
  AUTHORS   Good,M.E., Ek-Vitorin,J.F. and Burt,J.M.
  TITLE     Structural determinants and proliferative consequences of connexin
            37 hemichannel function in insulinoma cells
  JOURNAL   J Biol Chem 289 (44), 30379-30386 (2014)
   PUBMED   25217644
  REMARK    GeneRIF: Cx37-N55I and -Q58L mutations formed hemichannels with
            behavior and permeation properties similar to Cx37-WT (especially
            Q58L), but none of the mutants suppressed rat insulinoma cell
            proliferation.
REFERENCE   5  (bases 1 to 1617)
  AUTHORS   Ganesan,S. and Keating,A.F.
  TITLE     Impact of 7,12-dimethylbenz[a]anthracene exposure on connexin gap
            junction proteins in cultured rat ovaries
  JOURNAL   Toxicol Appl Pharmacol 274 (2), 209-214 (2014)
   PUBMED   24269759
  REMARK    GeneRIF: 7,12-Dimethylbenz[a]anthracene increases Cx37 and Cx43
            expression prior to follicle loss and during follicle loss both
            Cx37 and Cx43 expressions are reduced in cultured rat ovaries.
REFERENCE   6  (bases 1 to 1617)
  AUTHORS   Lin,S.H., Lu,C.Y., Muhammad,R., Chou,W.Y., Lin,F.C., Wu,P.C.,
            Lin,C.R. and Yang,L.C.
  TITLE     Induction of connexin 37 expression in a rat model of neuropathic
            pain
  JOURNAL   Brain Res Mol Brain Res 99 (2), 134-140 (2002)
   PUBMED   11978404
  REMARK    GeneRIF: Upregulation of connexin 37 mRNA after sciatic nerve
            injury causes thermal hyperalgesia; gap junctions thus may be
            responsible for this hyperexcitability.
REFERENCE   7  (bases 1 to 1617)
  AUTHORS   Yeh,H.I., Lai,Y.J., Chang,H.M., Ko,Y.S., Severs,N.J. and Tsai,C.H.
  TITLE     Multiple connexin expression in regenerating arterial endothelial
            gap junctions
  JOURNAL   Arterioscler Thromb Vasc Biol 20 (7), 1753-1762 (2000)
   PUBMED   10894813
REFERENCE   8  (bases 1 to 1617)
  AUTHORS   Yeh,H.I., Rothery,S., Dupont,E., Coppen,S.R. and Severs,N.J.
  TITLE     Individual gap junction plaques contain multiple connexins in
            arterial endothelium
  JOURNAL   Circ Res 83 (12), 1248-1263 (1998)
   PUBMED   9851942
REFERENCE   9  (bases 1 to 1617)
  AUTHORS   Vrionis,F.D., Wu,J.K., Qi,P., Waltzman,M., Cherington,V. and
            Spray,D.C.
  TITLE     The bystander effect exerted by tumor cells expressing the herpes
            simplex virus thymidine kinase (HSVtk) gene is dependent on
            connexin expression and cell communication via gap junctions
  JOURNAL   Gene Ther 4 (6), 577-585 (1997)
   PUBMED   9231074
REFERENCE   10 (bases 1 to 1617)
  AUTHORS   Haefliger,J.A., Bruzzone,R., Jenkins,N.A., Gilbert,D.J.,
            Copeland,N.G. and Paul,D.L.
  TITLE     Four novel members of the connexin family of gap junction proteins.
            Molecular cloning, expression, and chromosome mapping
  JOURNAL   J Biol Chem 267 (3), 2057-2064 (1992)
   PUBMED   1370487
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC078837.1.
            
            On Aug 8, 2004 this sequence version replaced NM_021654.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC078837.1, EX490393.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760494, SAMN16093043
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..1617
                     /gene="Gja4"
                     /gene_synonym="CXN37; Cxnh1"
                     /note="gap junction protein, alpha 4"
                     /db_xref="GeneID:25655"
                     /db_xref="RGD:2691"
     exon            1..60
                     /gene="Gja4"
                     /gene_synonym="CXN37; Cxnh1"
                     /inference="alignment:Splign:2.1.0"
     exon            61..1590
                     /gene="Gja4"
                     /gene_synonym="CXN37; Cxnh1"
                     /inference="alignment:Splign:2.1.0"
     CDS             78..1079
                     /gene="Gja4"
                     /gene_synonym="CXN37; Cxnh1"
                     /note="cx37; connexin-37; Gap junction membrane channel,
                     protein alpha 4 (connexin 37); connexin h1; gap junction
                     membrane channel protein alpha 4"
                     /codon_start=1
                     /product="gap junction alpha-4 protein"
                     /protein_id="NP_067686.1"
                     /db_xref="GeneID:25655"
                     /db_xref="RGD:2691"
                     /translation="MGDWGFLEKLLDQVQEHSTVVGKIWLTVLFIFRILILGLAGESV
                     WGDEQSDFECNTAQPGCTNVCYDQAFPISHIRYWVLQFLFVSTPTLIYLGHVIYLSRR
                     EERLRQKEGELRALPSKDPHVERALAAIEHQMAKISVAEDGRLRIRGALMGTYVISVL
                     CKSVLEAGFLYGQWRLYGWTMEPVFVCQRAPCPHVVDCYVSRPTEKTIFIIFMLVVGV
                     ISLVLNLLELVHLLCRCVSREIKARRDHDTRPAQGSASDPYPEQVFFYLPMGEGPSSP
                     PCPTYNGLSSTEQNWANLTTEERLTSTRPPPFVNAAPQGGQKSSSRPNSSASKKQYV"
ORIGIN      
        1 cgcaggcgca gcttcggcca cggtcgtccc ctctacctgg gccagccggg caagcaggcg
       61 agagaggccc cggaaacatg ggtgactggg gcttcctgga gaagctgcta gaccaggtcc
      121 aggagcactc gaccgtggtg ggcaagatct ggttaacggt gctcttcatc ttccgaatcc
      181 tcatcctggg cctggctggt gagtcggtgt ggggcgacga gcagtctgat ttcgaatgta
      241 acacagccca gccaggctgc accaatgtgt gctatgacca ggctttcccc atctcccaca
      301 tccgatactg ggtgctgcag ttcctcttcg tgagcacacc caccctgatc tacctgggcc
      361 acgtcattta cctgtctcgg cgggaagaac ggttgcggca gaaagaggga gagcttcggg
      421 cactgccatc caaggaccct catgtagagc gggcactagc agccatagaa catcagatgg
      481 ccaagatctc ggtggcagag gacggtcgtc ttcgcattcg aggggcgctc atgggtacct
      541 acgtgatcag tgtgctgtgt aagagtgtgc tggaggcagg cttcctctat ggccagtggc
      601 gtctctacgg ctggaccatg gagccggtgt ttgtgtgcca gcgtgcgccc tgcccccacg
      661 tcgtggactg ctacgtctct cgacccaccg agaagaccat cttcatcatt ttcatgttgg
      721 tggtaggggt catctccttg gtgctcaacc tactggagct ggtgcacctg ttgtgccgct
      781 gtgtcagccg ggagataaag gcacggaggg accatgacac ccgcccggct cagggcagtg
      841 cctcagaccc ttaccctgaa caggttttct tctacctccc catgggcgag ggaccctcgt
      901 ccccaccgtg tcccacctac aatgggctct catccactga gcaaaactgg gccaatttga
      961 ccaccgagga gagactgacc tctaccagac ctcccccatt tgtaaatgca gccccccagg
     1021 gtggccagaa gtcctctagc cgccccaaca gctctgcatc caagaagcag tatgtatagg
     1081 gctctggggc ttctgtcact ccacaggcta tcctgaggag tgtgtagtac cttgaatgcc
     1141 tctcgggcgg tccactcctc aaaggcactt gagagacaac tgggcctgga aggtggacat
     1201 tcgctgggca gaagacctga tcacaggtgg ttctggaata ccctgagcct tagtgatgac
     1261 caaaggacac tggcttcttt ccagaatgtg accctaccag cctcggtgac agcaggtggg
     1321 gtgctcttgg ctaagtgtgc tgtgaactct ctgatccttt tcgcctgatg cagagggatc
     1381 ccgagccaag tcgacttcag gtttccatca caccccactt ctcctgggag aaggcactga
     1441 tgtaggctgc tggctcagct tcgtcaccag gacagacaca gcctgcctgc gctggatctg
     1501 gccctttcca ggggagctgg ttggtgtctt gtttccctca ctcctagttc cactgtttat
     1561 gatcctaaaa taaacgggac tccatcataa aaaaaaaaaa aaaaaaaaaa aaaaaaa
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