LOCUS       NM_024376               1590 bp    mRNA    linear   ROD 29-MAR-2024
DEFINITION  Rattus norvegicus gap junction protein, alpha 3 (Gja3), mRNA.
ACCESSION   NM_024376
VERSION     NM_024376.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abrams,C.K., Peinado,A., Mahmoud,R., Bocarsly,M., Zhang,H.,
            Chang,P., Botello-Smith,W.M., Freidin,M.M. and Luo,Y.
  TITLE     Alterations at Arg76 of human connexin 46, a residue associated
            with cataract formation, cause loss of gap junction formation but
            preserve hemichannel function
  JOURNAL   Am J Physiol Cell Physiol 315 (5), C623-C635 (2018)
   PUBMED   30044662
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Pinto,B.I., Pupo,A., Garcia,I.E., Mena-Ulecia,K., Martinez,A.D.,
            Latorre,R. and Gonzalez,C.
  TITLE     Calcium binding and voltage gating in Cx46 hemichannels
  JOURNAL   Sci Rep 7 (1), 15851 (2017)
   PUBMED   29158540
  REMARK    GeneRIF: In agreement with the model and suggesting a
            conformational change that narrows the pore, Ca(2+) inhibits the
            water flux through Cx hemichannels. We conclude that Ca(2+) and
            voltage act allosterically to stabilize the closed conformation of
            Cx46 hemichannels.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1590)
  AUTHORS   Pinto,B.I., Garcia,I.E., Pupo,A., Retamal,M.A., Martinez,A.D.,
            Latorre,R. and Gonzalez,C.
  TITLE     Charged Residues at the First Transmembrane Region Contribute to
            the Voltage Dependence of the Slow Gate of Connexins
  JOURNAL   J Biol Chem 291 (30), 15740-15752 (2016)
   PUBMED   27143357
  REMARK    GeneRIF: the role of the charged residues at the end of TM-1 in
            voltage sensing in Cx26, Cx46, and Cx50.
REFERENCE   4  (bases 1 to 1590)
  AUTHORS   Schlingmann,B., Schadzek,P., Hemmerling,F., Schaarschmidt,F.,
            Heisterkamp,A. and Ngezahayo,A.
  TITLE     The role of the C-terminus in functional expression and
            internalization of rat connexin46 (rCx46)
  JOURNAL   J Bioenerg Biomembr 45 (1-2), 59-70 (2013)
   PUBMED   23065326
  REMARK    GeneRIF: These results suggest that the C-terminal region of rCx46
            up to A311 (rCx46(35.3)) is necessary for its correct
            compartmentalization and internalization in the form of annular
            junctions
REFERENCE   5  (bases 1 to 1590)
  AUTHORS   Banerjee,D., Das,S., Molina,S.A., Madgwick,D., Katz,M.R., Jena,S.,
            Bossmann,L.K., Pal,D. and Takemoto,D.J.
  TITLE     Investigation of the reciprocal relationship between the expression
            of two gap junction connexin proteins, connexin46 and connexin43
  JOURNAL   J Biol Chem 286 (27), 24519-24533 (2011)
   PUBMED   21606502
  REMARK    GeneRIF: Cx43 and Cx46 have novel functions in regulating each
            other's expression and turnover in a reciprocal manner in addition
            to their conventional roles as gap junction proteins in lens cells.
REFERENCE   6  (bases 1 to 1590)
  AUTHORS   White,T.W.
  TITLE     Unique and redundant connexin contributions to lens development
  JOURNAL   Science 295 (5553), 319-320 (2002)
   PUBMED   11786642
REFERENCE   7  (bases 1 to 1590)
  AUTHORS   Ngezahayo,A., Zeilinger,C., Todt,I., Marten,I. and Kolb,H.A.
  TITLE     Inactivation of expressed and conducting rCx46 hemichannels by
            phosphorylation
  JOURNAL   Pflugers Arch 436 (4), 627-629 (1998)
   PUBMED   9683738
  REMARK    GeneRIF: Data provide evidence that the activation and inhibition
            of PKC affect the rCx46-mediated membrane conductance as well as
            the voltage-dependent current inactivation in an inverse manner.
REFERENCE   8  (bases 1 to 1590)
  AUTHORS   Gao,Y. and Spray,D.C.
  TITLE     Structural changes in lenses of mice lacking the gap junction
            protein connexin43
  JOURNAL   Invest Ophthalmol Vis Sci 39 (7), 1198-1209 (1998)
   PUBMED   9620080
REFERENCE   9  (bases 1 to 1590)
  AUTHORS   Koval,M., Harley,J.E., Hick,E. and Steinberg,T.H.
  TITLE     Connexin46 is retained as monomers in a trans-Golgi compartment of
            osteoblastic cells
  JOURNAL   J Cell Biol 137 (4), 847-857 (1997)
   PUBMED   9151687
REFERENCE   10 (bases 1 to 1590)
  AUTHORS   Paul,D.L., Ebihara,L., Takemoto,L.J., Swenson,K.I. and
            Goodenough,D.A.
  TITLE     Connexin46, a novel lens gap junction protein, induces
            voltage-gated currents in nonjunctional plasma membrane of Xenopus
            oocytes
  JOURNAL   J Cell Biol 115 (4), 1077-1089 (1991)
   PUBMED   1659572
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000015.1.
            
            On Mar 15, 2021 this sequence version replaced NM_024376.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X57970.1, CK358480.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760386, SAMEA5760475
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-199               JAXUCZ010000015.1  35322207-35322405   c
            200-1590            JAXUCZ010000015.1  35296946-35298336   c
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="15"
                     /map="15p12"
     gene            1..1590
                     /gene="Gja3"
                     /gene_synonym="Cxnj1"
                     /note="gap junction protein, alpha 3"
                     /db_xref="GeneID:79217"
                     /db_xref="RGD:621820"
     exon            1..199
                     /gene="Gja3"
                     /gene_synonym="Cxnj1"
                     /inference="alignment:Splign:2.1.0"
     exon            200..1590
                     /gene="Gja3"
                     /gene_synonym="Cxnj1"
                     /inference="alignment:Splign:2.1.0"
     CDS             217..1467
                     /gene="Gja3"
                     /gene_synonym="Cxnj1"
                     /note="cx46; connexin-46; gap junction membrane channel
                     protein alpha 3; connexin j1"
                     /codon_start=1
                     /product="gap junction alpha-3 protein"
                     /protein_id="NP_077352.2"
                     /db_xref="GeneID:79217"
                     /db_xref="RGD:621820"
                     /translation="MGDWSFLGRLLENAQEHSTVIGKVWLTVLFIFRILVLGAAAEEV
                     WGDEQSDFTCNTQQPGCENVCYDRAFPISHIRFWALQIIFVSTPTLIYLGHVLHIVRM
                     EEKKKEREEELLRRDNPQHGRGREPMRTGSPRDPPLRDDRGKVRIAGALLRTYVFNII
                     FKTLFEVGFIAGQYFLYGFQLQPLYRCDRWPCPNTVDCFISRPTEKTIFVIFMLAVAC
                     ASLVLNMLEIYHLGWKKLKQGVTNHFNPDASEVRHKPLDPLSEAANSGPPSVSIGLPP
                     YYTHPACPTVQAKATGFPGAPLLPADFTVVTLNDAQGRGHPVKHCNGHHLTTEQNWAS
                     LGAEPQTPASKPSSAASSPHGRKGLTDSSGSSLEESALVVTPEGEQALATTVEMHSPP
                     LVLLDPERSSKSSSGRARPGDLAI"
ORIGIN      
        1 cggcggggca gcggcgtggg gcaccaagtg cggtgcgtac cgcggcggtg gggatggcgg
       61 ccggggcgga gaagcccggc cctggcgcgg ggcgctcgtg ggaagaaggg gcgccgcacg
      121 agtgaagagg gagcctcctt tacagagcag gccgagcccg gctgggcgag gagcgccggt
      181 gccgccgcgg cagagcgggg ttagcagtcg ggagcaatgg gcgactggag cttcctgggg
      241 cggctgctgg agaatgcgca ggagcactct acagtcatcg gcaaggtgtg gctgaccgtc
      301 ctgttcatct tccgcattct ggtgctgggg gcggcagccg aggaggtgtg gggtgatgag
      361 cagtcggact tcacctgcaa cacacagcag ccaggctgtg agaacgtctg ctacgaccgt
      421 gccttcccca tctcgcacat tcgcttctgg gcgctgcaaa tcatcttcgt gtccacgccc
      481 accctcatct atctgggcca cgtgctgcac atcgtgcgca tggaggagaa gaagaaagag
      541 cgggaggagg agctgctgag gagagacaac cctcagcacg gccgtggtcg ggaaccaatg
      601 cgtacaggga gcccgcggga cccgccactg cgcgatgacc gtggcaaggt gcgcatcgca
      661 ggcgcgctgc tgcggaccta cgtcttcaac atcatcttca agacgctctt cgaagtgggg
      721 ttcatcgcgg gccagtactt tctatatggc ttccagctgc agccgcttta ccgctgcgac
      781 cgctggccct gccccaacac cgtggactgc ttcatctcca ggcccacgga gaagaccatc
      841 tttgttatct tcatgttggc tgtggcctgt gcgtcactgg tgctcaacat gctagagatt
      901 taccacctgg gctggaagaa gctcaagcag ggagttacca accacttcaa cccagatgcc
      961 tcagaagtca ggcacaagcc cttggacccc ctatccgagg ccgccaactc tggccctccc
     1021 agcgtctcca ttgggttgcc accttattac acacaccctg cctgtcccac agtacaggca
     1081 aaggccacag ggtttcctgg ggccccactg ctaccagcag acttcacagt ggtgacccta
     1141 aacgatgcgc aaggcagagg ccacccggtc aagcactgca atggccacca cctgacgaca
     1201 gagcagaact gggccagcct aggggcagag ccgcagactc cagccagcaa gccctcttca
     1261 gcagcctcca gccctcatgg ccgcaagggg ctcactgaca gcagtggcag cagcttagag
     1321 gagagtgcct tggtggtgac accagaggga gagcaggctt tggcgaccac agtggagatg
     1381 cactccccac cgttggtcct cctggaccca gaaaggtcca gcaagtccag cagcggacga
     1441 gccagaccag gtgacttggc catctagcca cagtctctcc agataccttt ataaaccttc
     1501 tctttacccc ttagaaacca aaaccaaacc aaaccaaaac aaaacagaac caaaaagcat
     1561 ttcaaatcaa caggtagaaa gagaaaaaac
//
