LOCUS       NM_001100505            1365 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Rattus norvegicus guided entry of tail-anchored proteins factor 3,
            ATPase (Get3), mRNA.
ACCESSION   NM_001100505 XM_001066031 XM_213848
VERSION     NM_001100505.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Colombo,S.F., Cardani,S., Maroli,A., Vitiello,A., Soffientini,P.,
            Crespi,A., Bram,R.F., Benfante,R. and Borgese,N.
  TITLE     Tail-anchored Protein Insertion in Mammals: FUNCTION AND RECIPROCAL
            INTERACTIONS OF THE TWO SUBUNITS OF THE TRC40 RECEPTOR
  JOURNAL   J Biol Chem 291 (29), 15292-15306 (2016)
   PUBMED   27226539
  REMARK    GeneRIF: investigated the role of endogenous WRB and CAML in Asna1
            tail-anchored protein insertion as well as their association,
            concentration, and stoichiometry in liver microsomes
            Erratum:[J Biol Chem. 2016 Sep 2;291(36):18855. doi:
            10.1074/jbc.A115.707752.. PMID: 27591255]
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Mock,J.Y., Chartron,J.W., Zaslaver,M., Xu,Y., Ye,Y. and
            Clemons,W.M. Jr.
  TITLE     Bag6 complex contains a minimal tail-anchor-targeting module and a
            mock BAG domain
  JOURNAL   Proc Natl Acad Sci U S A 112 (1), 106-111 (2015)
   PUBMED   25535373
REFERENCE   3  (bases 1 to 1365)
  AUTHORS   Yamamoto,Y. and Sakisaka,T.
  TITLE     Molecular machinery for insertion of tail-anchored membrane
            proteins into the endoplasmic reticulum membrane in mammalian cells
  JOURNAL   Mol Cell 48 (3), 387-397 (2012)
   PUBMED   23041287
REFERENCE   4  (bases 1 to 1365)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB700861.1, BC098819.1 and AA997032.1.
            
            On or before Nov 21, 2008 this sequence version replaced
            XM_213848.4, XM_001066031.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC098819.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-301               CB700861.1         1-301
            302-1257            BC098819.1         237-1192
            1258-1365           AA997032.1         1-108               c
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="19"
                     /map="19q11"
     gene            1..1365
                     /gene="Get3"
                     /gene_synonym="Asna1"
                     /note="guided entry of tail-anchored proteins factor 3,
                     ATPase"
                     /db_xref="GeneID:288919"
                     /db_xref="RGD:1307906"
     exon            1..171
                     /gene="Get3"
                     /gene_synonym="Asna1"
                     /inference="alignment:Splign:2.1.0"
     CDS             11..1057
                     /gene="Get3"
                     /gene_synonym="Asna1"
                     /EC_number="7.3.2.7"
                     /note="ATPase ASNA1; arsenite-stimulated ATPase; arsenical
                     pump-driving ATPase; arsA arsenite transporter,
                     ATP-binding, homolog 1"
                     /codon_start=1
                     /product="ATPase GET3"
                     /protein_id="NP_001093975.1"
                     /db_xref="GeneID:288919"
                     /db_xref="RGD:1307906"
                     /translation="MAAGVAGWGVEAEEFEDAPDVEPLEPTLSNIIEQRSLKWIFVGG
                     KGGVGKTTCSCSLAVQLSKGRESVLIISTDPAHNISDAFDQKFSKVPTKVKGYDNLFA
                     MEIDPSLGVAELPDEFFEEDNMLSMGKKMMQEAMSAFPGIDEAMSYAEVMRLVKGMNF
                     SVVVFDTAPTGHTLRLLNFPTIVERGLGRLMQIKNQISPFISQMCNMLGLGDMNADQL
                     ASKLEETLPVIRSVSEQFKDPEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNII
                     VNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTF
                     SALLLEPYKPPSTQ"
     exon            172..319
                     /gene="Get3"
                     /gene_synonym="Asna1"
                     /inference="alignment:Splign:2.1.0"
     exon            320..468
                     /gene="Get3"
                     /gene_synonym="Asna1"
                     /inference="alignment:Splign:2.1.0"
     exon            469..619
                     /gene="Get3"
                     /gene_synonym="Asna1"
                     /inference="alignment:Splign:2.1.0"
     exon            620..727
                     /gene="Get3"
                     /gene_synonym="Asna1"
                     /inference="alignment:Splign:2.1.0"
     exon            728..925
                     /gene="Get3"
                     /gene_synonym="Asna1"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1351
                     /gene="Get3"
                     /gene_synonym="Asna1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgggtccaag atggcggcgg gggtggccgg gtggggggtt gaagcagaag agttcgagga
       61 tgcacctgac gtggagccac tggaacccac gcttagcaat atcatcgagc agcgtagcct
      121 taagtggatc ttcgtcgggg gcaaaggagg cgttggtaag accacctgca gctgcagcct
      181 ggcggtccag ctgtctaagg gacgggagag tgttctcatc atttctacag acccagctca
      241 taacatttca gatgcttttg atcagaagtt ctccaaggtg cctaccaagg tcaaaggcta
      301 tgacaacctc tttgctatgg agatagaccc cagcctgggc gttgcagagc tccccgacga
      361 gttcttcgag gaagacaaca tgctgagcat gggcaagaag atgatgcagg aggccatgag
      421 cgccttccct ggcatcgatg aggccatgag ttatgctgag gtcatgaggc tggtgaaagg
      481 catgaacttc tcagtggtgg tgttcgacac agcacccacc ggccatacac tcaggctcct
      541 gaacttcccc accatcgtgg agcggggcct gggccgcctg atgcagatca agaaccagat
      601 cagccccttc atctcacaga tgtgcaacat gctgggtctg ggggacatga acgctgacca
      661 gctggcctcc aagttagaag agaccttgcc cgtcatccga tccgtcagcg aacagttcaa
      721 ggaccctgaa cagacgacct tcatctgtgt gtgcatcgcc gagtttttgt ccctgtatga
      781 gacggagcgt ctgatccagg agctggccaa gtgcaagatc gacacccaca acatcatcgt
      841 caaccagctt gtcttccccg accctgagaa accctgcaag atgtgtgagg cccgacacaa
      901 gatccaggcc aaatacctgg accagatgga agacctctat gaagactttc acattgtaaa
      961 gctgccactg ttacctcacg aggttcgggg agccgacaaa gtcaacacct tctctgccct
     1021 cctcctggag ccctacaagc cccccagcac ccagtagcca accccattcc agctgctgcc
     1081 acttcatacc ctcctctttc cccacggggc agagtttgca caaagtcccc cataatgcag
     1141 ggggagccac ttaggaggtg ggagggggtc tgttccccct ggtggggcct ggggaggctg
     1201 caatgccccc ccacctcttc ctgcctcata ctcctcaata aaataatctt aaacagctgt
     1261 gtttgtccgt ggagggtggg taggtaagca cttgtgtgat gattctgcac gagtgtttgg
     1321 ctccctctca cacaggtcca tccatagcaa aaaaaaaaaa aaaaa
//
