LOCUS       NM_001106717            4285 bp    mRNA    linear   ROD 20-OCT-2024
DEFINITION  Rattus norvegicus GEN1 Holliday junction 5' flap endonuclease
            (Gen1), transcript variant 2, mRNA.
ACCESSION   NM_001106717 XM_001072749 XM_233966
VERSION     NM_001106717.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Shah Punatar,R., Martin,M.J., Wyatt,H.D., Chan,Y.W. and West,S.C.
  TITLE     Resolution of single and double Holliday junction recombination
            intermediates by GEN1
  JOURNAL   Proc Natl Acad Sci U S A 114 (3), 443-450 (2017)
   PUBMED   28049850
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Lee,S.H., Princz,L.N., Klugel,M.F., Habermann,B., Pfander,B. and
            Biertumpfel,C.
  TITLE     Human Holliday junction resolvase GEN1 uses a chromodomain for
            efficient DNA recognition and cleavage
  JOURNAL   Elife 4, e12256 (2015)
   PUBMED   26682650
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4285)
  AUTHORS   Chan,Y.W. and West,S.
  TITLE     GEN1 promotes Holliday junction resolution by a coordinated nick
            and counter-nick mechanism
  JOURNAL   Nucleic Acids Res 43 (22), 10882-10892 (2015)
   PUBMED   26578604
REFERENCE   4  (bases 1 to 4285)
  AUTHORS   Garner,E., Kim,Y., Lach,F.P., Kottemann,M.C. and Smogorzewska,A.
  TITLE     Human GEN1 and the SLX4-associated nucleases MUS81 and SLX1 are
            essential for the resolution of replication-induced Holliday
            junctions
  JOURNAL   Cell Rep 5 (1), 207-215 (2013)
   PUBMED   24080495
REFERENCE   5  (bases 1 to 4285)
  AUTHORS   Rodrigue,A., Coulombe,Y., Jacquet,K., Gagne,J.P., Roques,C.,
            Gobeil,S., Poirier,G. and Masson,J.Y.
  TITLE     The RAD51 paralogs ensure cellular protection against mitotic
            defects and aneuploidy
  JOURNAL   J Cell Sci 126 (Pt 1), 348-359 (2013)
   PUBMED   23108668
REFERENCE   6  (bases 1 to 4285)
  AUTHORS   Gao,M., Rendtlew Danielsen,J., Wei,L.Z., Zhou,D.P., Xu,Q., Li,M.M.,
            Wang,Z.Q., Tong,W.M. and Yang,Y.G.
  TITLE     A novel role of human holliday junction resolvase GEN1 in the
            maintenance of centrosome integrity
  JOURNAL   PLoS One 7 (11), e49687 (2012)
   PUBMED   23166748
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH473947.1.
            
            On or before Oct 4, 2007 this sequence version replaced
            XM_233966.4, XM_001072749.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMD00132261, SAMD00132262
                              [ECO:0000350]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="6"
                     /map="6q15"
     gene            1..4285
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /note="GEN1 Holliday junction 5' flap endonuclease"
                     /db_xref="GeneID:298884"
                     /db_xref="RGD:1559792"
     exon            1..217
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            218..404
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     CDS             244..2970
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /note="isoform 2 is encoded by transcript variant 2; flap
                     endonuclease GEN homolog 1; Gen homolog 1, endonuclease;
                     Gen endonuclease homolog 1"
                     /codon_start=1
                     /product="flap endonuclease GEN homolog 1 isoform 2"
                     /protein_id="NP_001100187.1"
                     /db_xref="GeneID:298884"
                     /db_xref="RGD:1559792"
                     /translation="MGVNDLWQILEPVKQHIHLQDLCGKTIAVDLSLWVCEAQTVKKM
                     IGTVMKPHLRNLFFRISYLTQMNVKLVFVMEGEPPKLKADVMNKRTQTRYGPSGKSRS
                     QKTGRSHFKSVLRECLEMLECLGMPWVQAAGEAEAMCAYLNASGHVDGCLTNDGDAFL
                     YGAQTVYRNFTMNTKDPHVDCYTVSSIKSKLGLDRDALVGLAVLLGCDYLPKGVPGVG
                     KEQALKLLQILKGQSLLQRFNQWTEEPGYSVPQSAAKKVVHCSVCSHPGSPKDHERNG
                     CTLCKSDRYCEPHDYEYLCPCEWHQTDHNRQLNEIENNIKKKACSCEGFPFHEVIQEF
                     LSNKNKLLKPIRYQRPDLLLFQRFTVQKMEWPSHYACEKLLVLLTRYDMIERKLGRKT
                     SNQLQPIRIVKPRIRNGVCCLEIEWEKPEHYSVEDTQPGGLDLLTIEEASLFEAAYPE
                     VVAIYQKQQSETKGKKQKNIKIKPKGSHLPESDDVISSQSLITLEPISKAFPKQNPKF
                     DLEISPDPILAQESTSPSLSSFVSPENALGLNLQEQLMPSPRTLAVKQTKDVSNFLVS
                     ECSQPSSSSHNISVITDLHLSTIDWAGTSFSNSPAVQRNTFSQDLQSEPESSAILPAF
                     EQLSYESEQCTLSNKVSNRGLQKSGPEEHLLSSITGLHLHDLPLKERIHIKSSCPQYN
                     VGADADLESLPLTMNHSCIAYSSSGGSSHFSKDLTGVYLQKELRNSKVLDSHFLQENF
                     RVNTSLPYSFNDEAVKTSSLQVGLPTAAVPHNPKVDVKTKNLVKKSSVCLKRDSSDEE
                     NAAVSWKAKYTAPKLKHSSHNHSLVHDRDSTHNKHRNPKDESNETKLCTESFKIAEDE
                     ENKFSDPARNPQSFLQCHNKDEDSADCWESPLPLRQRLKLRFQNTQSRLYNT"
     exon            405..591
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            592..768
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            769..879
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            880..953
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1042
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1193
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1230
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1311
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1442
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            1443..1504
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..1648
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
     exon            1649..4285
                     /gene="Gen1"
                     /gene_synonym="RGD1559792"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tggcgatggc gctggattcg aactgctgcc gggagcttag tgcttgggag tgctgtgaga
       61 ggcttccccg ccctttggtc ttgtttgttt gtttgtttta actttggttt gagggaattc
      121 gggaacttag gggcagcgat agttattaga ctcctggcgg ccagattggt acatgacaga
      181 agtgttcctt ccacagcctc gaagaggggg tgtctggata acaccagata atcaaccatc
      241 aggatgggag tgaatgacct gtggcaaatt ttggagcctg taaagcaaca tatccacttg
      301 caggatcttt gtgggaagac cattgccgtt gatttgagtc tctgggtatg cgaggcacag
      361 acagtgaaga aaatgattgg aactgtcatg aagccccacc tcaggaacct attttttcgt
      421 atttcatatt taactcaaat gaatgtaaaa ctggtgtttg tgatggaagg ggagccacca
      481 aagctgaaag ctgatgtcat gaacaagagg actcagactc gatacgggcc ttctggaaaa
      541 tcgaggtctc agaaaacagg gagatcgcat tttaagtcag tcttacgaga gtgccttgaa
      601 atgctagagt gcctaggaat gccctgggtc caggctgcgg gggaagctga ggccatgtgt
      661 gcttacctta atgccagcgg ccatgtggac ggctgcctca ccaacgacgg cgatgctttc
      721 ctttatggag cccagactgt gtacaggaat ttcaccatga acacaaagga tccacatgtt
      781 gactgttaca cggtatcatc tatcaagagt aagctaggct tggatcgaga tgctctggtg
      841 ggactggcgg tacttcttgg ttgtgattac cttccgaagg gagtgcctgg agttggaaaa
      901 gaacaagcgt taaaactttt gcagattttg aaaggtcaaa gtttgcttca gaggtttaac
      961 cagtggactg aagaacctgg ctactctgtt ccacaatcag cagcaaaaaa agtggttcac
     1021 tgctcggtgt gttcccatcc aggttctcct aaggatcatg aacgcaatgg gtgcacacta
     1081 tgtaaaagtg atagatactg tgaaccacat gattatgaat acctctgtcc ttgtgaatgg
     1141 caccagacag accataatag gcaactaaat gaaatagaaa acaatattaa aaagaaagct
     1201 tgcagttgcg aaggatttcc attccatgag gtcatccaag aattcctttc gaataagaat
     1261 aaactgttaa aaccgatcag gtaccaaaga cctgatttat tattgtttca gagatttact
     1321 gttcaaaaaa tggagtggcc cagtcactat gcgtgtgaaa aattgttggt gcttttgacc
     1381 cgctatgaca tgatagaaag aaaacttggt agaaagactt ccaatcaact acagccaatt
     1441 agaattgtta aacctcgaat cagaaatgga gtctgttgtc ttgaaataga atgggaaaag
     1501 cctgaacatt attcggtaga agatacacaa cctggaggac tggatctcct tacaatcgaa
     1561 gaagcatcac tgtttgaagc agcctatcct gaagttgtag ctatctacca gaaacaacag
     1621 tcagaaacta aagggaagaa acagaaaaat ataaaaatta agcccaaagg aagccatcta
     1681 ccagaatcag atgatgtgat cagttcccag tcacttatta ctttagaacc tatatcaaaa
     1741 gccttcccca agcagaatcc caaatttgat ttggaaattt ctccagatcc tattttagca
     1801 caggagtcta cttctccctc attgagtagt tttgtttccc ctgaaaatgc tcttggtttg
     1861 aatttgcaag aacagttaat gccttctcca agaactttgg ctgtaaagca aactaaagat
     1921 gtcagtaatt ttctagtttc agagtgtagt cagcccagtt cttcatccca taatatttct
     1981 gtgattactg atctacactt gagtaccatt gactgggcag gtacttcttt tagtaattct
     2041 ccagcagttc aaagaaacac tttctctcaa gatttacaat cagaacctga atcatcagcc
     2101 atccttcctg cctttgaaca gttgtcatat gaatcagaac aatgtacctt aagcaacaaa
     2161 gtgtccaata gaggtcttca gaagagtggt cctgaagagc atctcctttc cagcatcact
     2221 ggtttacatc ttcacgattt gcctctaaag gaaagaatac atataaaatc ttcatgccct
     2281 cagtataatg taggagcaga tgctgacctg gaaagtttac ccctaacaat gaatcactct
     2341 tgcattgctt acagtagctc tggtggttca tcccattttt cgaaggatct tacaggggta
     2401 tatcttcaga aagagctcag aaactctaaa gttctagaca gccacttcct tcaagaaaac
     2461 ttcagggtga atacttcctt accttattct ttcaatgacg aagcagtaaa gacttccagt
     2521 cttcaagttg ggctgccaac tgctgctgta cctcataatc caaaagttga tgtgaaaact
     2581 aagaaccttg tcaagaagag tagtgtttgc ctcaagagag attcctcaga tgaagagaat
     2641 gctgcagtgt cctggaaagc taagtatact gctcctaaat tgaaacacag ctctcataat
     2701 catagcctag tccatgacag agacagtact cacaacaaac acagaaatcc gaaagatgaa
     2761 tctaacgaaa ccaaactgtg tactgagtct tttaaaatag ctgaagatga agaaaacaag
     2821 ttctcagatc cagcaagaaa tccccagagt tttctacaat gtcataacaa ggatgaggac
     2881 tctgctgact gctgggaaag tcctctccct ttacgccaga ggttaaagct caggttccag
     2941 aacactcaaa gtagacttta taatacttaa atttaatgtg tgtaaatgct ggtaacatga
     3001 gcagaagcca agcaaacttg ggtttggtag aaaatgtaca tgactatgtg ccatttaaat
     3061 caaaattgta attttaaaat gttaacagct aggtgtggct catgcctata atcacagcat
     3121 gtctgaggtt aaaagagtcc agaggatcat gcaccaggag ttcaaagtca tcttctggta
     3181 tatggagatt gtcagaccaa tctcagttat ctaagaaagg aaaaaaatta aaattttgac
     3241 tataaaagat gagcctctgg tgacaacttg aaatggtttt tttttttaga acatttgaat
     3301 gtcttcttga ggtatcttta tattatctag ctttaacata ttatttcttg acaaagtaaa
     3361 tgctttatca ttttttgttc atcttatgtt agggtctaaa gcaactgttt actgtctagc
     3421 catcctcaag atggtaacac tttataatgg tgttgcagta tcttttatga cccttcctaa
     3481 tggctgtatt tccccttcat gttaaagaac cattagcatg ctcttccact tttagtaatg
     3541 tcatgtttga ggagtgaaaa tggaagagga tgtgtagtga agtgtgattg tgaagtcttg
     3601 gtaaggaaag ttaccttata tacattcaga agttcacagg cactgtaagc aggaaatatt
     3661 ttaaatcaga tgtaaaccaa aagagaattg gaagcttcct caggatcaca cagtaataat
     3721 agtaatggtg tggatcccat ctttccagtt tggttcttgt gattttattt ttctgttttg
     3781 tttgtaatct atagaactgt tcccttttgt cttaaatttt agtttgcatg taatgttgac
     3841 tttaattcct ataagtctta aatcttacat aaattgtaaa aatcctaaag gcatgtagag
     3901 ttacagctgt ttgtatgtga ctggagcctg aaagtagagg atcctaagga atccatcaaa
     3961 aacgtattaa aagcaagcca ggcttggtga cacacttctt taatgccagc atttggaagg
     4021 cagaggtaag tggatctcta aattcaagac tagcctggta ggcagagtta gttccaggac
     4081 agccagggct acacagaatt cctgttttga aaaaacaaaa aaaaaatttt ttaaaccaaa
     4141 ataaaaacta ttaaaagtga agagtaagtt tgcaaggttg caaaattcaa aaaatagttt
     4201 actaactaca tagttgtagt tctgtatatt cagtgtatga ctgaaattta accaagaaga
     4261 ttaaagttac tctgtttaca acatg
//
