LOCUS       NM_031776               1460 bp    mRNA    linear   ROD 26-APR-2024
DEFINITION  Rattus norvegicus guanine deaminase (Gda), mRNA.
ACCESSION   NM_031776
VERSION     NM_031776.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Rodriguez,A.R., O'Neill,K.M., Swiatkowski,P., Patel,M.V. and
            Firestein,B.L.
  TITLE     Overexpression of cypin alters dendrite morphology, single neuron
            activity, and network properties via distinct mechanisms
  JOURNAL   J Neural Eng 15 (1), 016020 (2018)
   PUBMED   29091046
  REMARK    GeneRIF: Overexpression of cypin alters dendrite branching and
            effects mean spike rate over time.
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Fernandez,J.R., Welsh,W.J. and Firestein,B.L.
  TITLE     Structural characterization of the zinc binding domain in cytosolic
            PSD-95 interactor (cypin): Role of zinc binding in guanine
            deamination and dendrite branching
  JOURNAL   Proteins 70 (3), 873-881 (2008)
   PUBMED   17803218
  REMARK    GeneRIF: Identification of several histidines and aspartic acid
            residues of cypin responsible for zinc binding.
REFERENCE   3  (bases 1 to 1460)
  AUTHORS   Chen,H. and Firestein,B.L.
  TITLE     RhoA regulates dendrite branching in hippocampal neurons by
            decreasing cypin protein levels
  JOURNAL   J Neurosci 27 (31), 8378-8386 (2007)
   PUBMED   17670984
  REMARK    GeneRIF: Cypin protein acts downstream of the small GTPase RhoA
            protein to regulate dendrite branching in hippocampal neurons.
REFERENCE   4  (bases 1 to 1460)
  AUTHORS   Sannomiya,K., Honda,H., Kubo,K., Ii,K., Yuan,Y., Aoyagi,E.,
            Muguruma,N., Shimizu,I. and Ito,S.
  TITLE     A histochemical and immunohistochemical investigation of guanase
            and nedasin in rat and human tissues
  JOURNAL   J Med Invest 53 (3-4), 246-254 (2006)
   PUBMED   16953061
  REMARK    GeneRIF: the experiments on guanase and nedasin in rat organs
            performed in this study are considered to have important
            implications in the investigation of their physiological
            significance
REFERENCE   5  (bases 1 to 1460)
  AUTHORS   Akum,B.F., Chen,M., Gunderson,S.I., Riefler,G.M.,
            Scerri-Hansen,M.M. and Firestein,B.L.
  TITLE     Cypin regulates dendrite patterning in hippocampal neurons by
            promoting microtubule assembly
  JOURNAL   Nat Neurosci 7 (2), 145-152 (2004)
   PUBMED   14730308
REFERENCE   6  (bases 1 to 1460)
  AUTHORS   Kuwahara,H., Araki,N., Makino,K., Masuko,N., Honda,S., Kaibuchi,K.,
            Fukunaga,K., Miyamoto,E., Ogawa,M. and Saya,H.
  TITLE     A novel NE-dlg/SAP102-associated protein, p51-nedasin, related to
            the amidohydrolase superfamily, interferes with the association
            between NE-dlg/SAP102 and N-methyl-D-aspartate receptor
  JOURNAL   J Biol Chem 274 (45), 32204-32214 (1999)
   PUBMED   10542258
REFERENCE   7  (bases 1 to 1460)
  AUTHORS   Firestein,B.L., Firestein,B.L., Brenman,J.E., Aoki,C.,
            Sanchez-Perez,A.M., El-Husseini,A.E. and Bredt,D.S.
  TITLE     Cypin: a cytosolic regulator of PSD-95 postsynaptic targeting
  JOURNAL   Neuron 24 (3), 659-672 (1999)
   PUBMED   10595517
REFERENCE   8  (bases 1 to 1460)
  AUTHORS   Yuan,G., Bin,J.C., McKay,D.J. and Snyder,F.F.
  TITLE     Cloning and characterization of human guanine deaminase.
            Purification and partial amino acid sequence of the mouse protein
  JOURNAL   J Biol Chem 274 (12), 8175-8180 (1999)
   PUBMED   10075721
REFERENCE   9  (bases 1 to 1460)
  AUTHORS   Durak,I., Cetin,R., Canbolat,O., Cetin,D., Yurtarslani,Z. and
            Unal,A.
  TITLE     Adenosine deaminase, 5'-nucleotidase, guanase and cytidine
            deaminase activities in gastric tissues from patients with gastric
            cancer
  JOURNAL   Cancer Lett 84 (2), 199-202 (1994)
   PUBMED   8076377
REFERENCE   10 (bases 1 to 1460)
  AUTHORS   Craft,C.M., Whitmore,D.H. and Wiechmann,A.F.
  TITLE     Cone arrestin identified by targeting expression of a functional
            family
  JOURNAL   J Biol Chem 269 (6), 4613-4619 (1994)
   PUBMED   8308033
  REMARK    Erratum:[J Biol Chem 1994 Jul 1;269(26):17756]
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF245172.1.
            
            On Jun 10, 2007 this sequence version replaced NM_031776.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF245172.1, FQ233257.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN16676809 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q51"
     gene            1..1460
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /note="guanine deaminase"
                     /db_xref="GeneID:83585"
                     /db_xref="RGD:621617"
     exon            1..169
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..1411
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /EC_number="3.5.4.3"
                     /note="GAH; guanase; guanine aminase; guanine
                     aminohydrolase"
                     /codon_start=1
                     /product="guanine deaminase"
                     /protein_id="NP_113964.2"
                     /db_xref="GeneID:83585"
                     /db_xref="RGD:621617"
                     /translation="MCAARTPQLALIFRGTFVHSTWTCPMEVLRDHLLGVSDSGKIVF
                     LEESSQQEKLAKEWCFKPCEIRELSHHEFFMPGLVDTHIHAPQYAFAGSNVDLPLLDW
                     LNKYTFPTEKRFQSTDVAEEVYTRVVRRTLKNGTTTACYFGTIHTDSSLILAEITDKF
                     GQRAFVGKVCMDLNNTVPEYKETTEESVKETERFVSEMLQKNYSRVKPIVTPRFSLSC
                     TETLMSELGNIAKTHDLYIQSHISENREEIEAVKSLYPGYKNYTDVYDKNNLLTNKTV
                     MAHGCYLSEEELNVFSERGASIAHCPNSNLSLSSGLLNVLDVLKHKVKIGLGTDVAGG
                     YSYSMLDAIRRAVMVSNVLLINKVNEKSLTLKEVFRLATLGGSQALGLDREIGNFEVG
                     KDFDALLINPRASDSPIDLFCGDFVGDISEAVIQKFLYLGDDRNIEEVYVGGKQVVPF
                     SSSV"
     exon            170..258
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            259..430
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            431..518
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            519..624
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            625..652
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            653..760
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            761..868
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            869..966
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1034
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1181
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1312
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1340
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1460
                     /gene="Gda"
                     /gene_synonym="Cypin"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggtgcaccct ctttggtcag tgaacttgcg ctgccccgct gctgccatgt gtgcggctcg
       61 gacgccgcag ctggcgctca tcttccgagg gactttcgtc cactccacct ggacctgccc
      121 catggaggtg cttcgcgatc accttcttgg cgtgagcgac agcggcaaaa tagtgtttct
      181 agaagaatca tctcagcaag aaaagctggc caaagaatgg tgcttcaaac cgtgtgagat
      241 cagagagctg agccaccatg agttcttcat gccaggcctt gttgatacac acatccatgc
      301 ccctcagtat gcctttgctg gaagcaacgt cgacctgcca cttttggatt ggctgaacaa
      361 gtatacattt cctacagaaa aaaggttcca gagcaccgat gtggctgaag aagtctacac
      421 tagagtcgtt aggagaacac taaagaacgg caccaccacg gcatgctact ttggaacaat
      481 tcacactgac tcatccctga tccttgcgga aattacagat aaatttgggc agcgagcatt
      541 tgtgggcaaa gtatgcatgg atttaaataa cactgttcca gaatacaagg agaccaccga
      601 ggagtcagtc aaggagacag agagatttgt gtcagaaatg cttcaaaaga attattcaag
      661 ggtgaaaccc atagtgaccc cgcgattttc tctttcttgc acggagactc tgatgagtga
      721 acttggcaac atcgccaaga ctcatgatct gtacatccag agccatataa gtgaaaatcg
      781 tgaagaaatt gaagctgtga aaagcttata ccctggctac aaaaactaca cagatgtcta
      841 tgataaaaac aatcttctga caaacaaaac agtgatggct catggctgct acctttctga
      901 agaagagctg aacgtcttca gtgaacgagg agcatccatt gcacattgtc ccaactctaa
      961 tctgtcgctg agcagtggct tactgaacgt gctcgatgtc ctgaagcata aagtgaagat
     1021 agggcttggg acagatgtgg ctggtggtta ctcctattcc atgcttgacg ccatccgaag
     1081 agcagtgatg gtttccaacg tcctcttaat taataaggtg aatgagaaaa gcctcaccct
     1141 caaagaagtc ttcagactag ccactcttgg aggaagccaa gccctggggc ttgatcgtga
     1201 aattggaaac tttgaggtcg gcaaggattt tgatgccctc ttgatcaacc ccagagcatc
     1261 ggactctccc attgatctgt tttgtgggga tttcgttggt gatatttctg aggctgttat
     1321 ccagaagttc ctctatctag gagatgaccg aaacattgag gaggtttatg tgggtggaaa
     1381 gcaggtcgtt ccattctcca gctccgtgta aggcccttgg acatccatga cgctctcctg
     1441 ggaggacatg agtctgctat
//
