LOCUS       NM_001168664            8538 bp    mRNA    linear   ROD 27-MAR-2024
DEFINITION  Rattus norvegicus GCN1 activator of EIF2AK4 (Gcn1), mRNA.
ACCESSION   NM_001168664 XM_001072034 XM_001072108
VERSION     NM_001168664.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Guo,Z., Neilson,L.J., Zhong,H., Murray,P.S., Zanivan,S. and
            Zaidel-Bar,R.
  TITLE     E-cadherin interactome complexity and robustness resolved by
            quantitative proteomics
  JOURNAL   Sci Signal 7 (354), rs7 (2014)
   PUBMED   25468996
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Cambiaghi,T.D., Pereira,C.M., Shanmugam,R., Bolech,M., Wek,R.C.,
            Sattlegger,E. and Castilho,B.A.
  TITLE     Evolutionarily conserved IMPACT impairs various stress responses
            that require GCN1 for activating the eIF2 kinase GCN2
  JOURNAL   Biochem Biophys Res Commun 443 (2), 592-597 (2014)
   PUBMED   24333428
REFERENCE   3  (bases 1 to 8538)
  AUTHORS   Roffe,M., Hajj,G.N., Azevedo,H.F., Alves,V.S. and Castilho,B.A.
  TITLE     IMPACT is a developmentally regulated protein in neurons that
            opposes the eukaryotic initiation factor 2alpha kinase GCN2 in the
            modulation of neurite outgrowth
  JOURNAL   J Biol Chem 288 (15), 10860-10869 (2013)
   PUBMED   23447528
REFERENCE   4  (bases 1 to 8538)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   5  (bases 1 to 8538)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000012.1.
            
            On or before Dec 13, 2009 this sequence version replaced
            XM_001072108.1, XM_001072034.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643429.43501.1,
                                           SRR26643299.50918.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                JAXUCZ010000012.1  46713272-46713289   c
            19-121              JAXUCZ010000012.1  46706442-46706544   c
            122-185             JAXUCZ010000012.1  46702942-46703005   c
            186-317             JAXUCZ010000012.1  46702314-46702445   c
            318-426             JAXUCZ010000012.1  46701863-46701971   c
            427-525             JAXUCZ010000012.1  46699424-46699522   c
            526-660             JAXUCZ010000012.1  46699202-46699336   c
            661-729             JAXUCZ010000012.1  46698608-46698676   c
            730-838             JAXUCZ010000012.1  46698389-46698497   c
            839-913             JAXUCZ010000012.1  46697780-46697854   c
            914-1042            JAXUCZ010000012.1  46697274-46697402   c
            1043-1093           JAXUCZ010000012.1  46696818-46696868   c
            1094-1192           JAXUCZ010000012.1  46696505-46696603   c
            1193-1366           JAXUCZ010000012.1  46696010-46696183   c
            1367-1519           JAXUCZ010000012.1  46695076-46695228   c
            1520-1612           JAXUCZ010000012.1  46692465-46692557   c
            1613-1688           JAXUCZ010000012.1  46690368-46690443   c
            1689-1848           JAXUCZ010000012.1  46689964-46690123   c
            1849-2038           JAXUCZ010000012.1  46688990-46689179   c
            2039-2163           JAXUCZ010000012.1  46688783-46688907   c
            2164-2342           JAXUCZ010000012.1  46688494-46688672   c
            2343-2436           JAXUCZ010000012.1  46687789-46687882   c
            2437-2550           JAXUCZ010000012.1  46686634-46686747   c
            2551-2749           JAXUCZ010000012.1  46686313-46686511   c
            2750-2905           JAXUCZ010000012.1  46685041-46685196   c
            2906-3087           JAXUCZ010000012.1  46684561-46684742   c
            3088-3168           JAXUCZ010000012.1  46683693-46683773   c
            3169-3312           JAXUCZ010000012.1  46683348-46683491   c
            3313-3440           JAXUCZ010000012.1  46681936-46682063   c
            3441-3630           JAXUCZ010000012.1  46681667-46681856   c
            3631-3701           JAXUCZ010000012.1  46681458-46681528   c
            3702-3867           JAXUCZ010000012.1  46680191-46680356   c
            3868-4062           JAXUCZ010000012.1  46679761-46679955   c
            4063-4309           JAXUCZ010000012.1  46677071-46677317   c
            4310-4431           JAXUCZ010000012.1  46675602-46675723   c
            4432-4546           JAXUCZ010000012.1  46674760-46674874   c
            4547-4726           JAXUCZ010000012.1  46673611-46673790   c
            4727-4947           JAXUCZ010000012.1  46668664-46668884   c
            4948-5016           JAXUCZ010000012.1  46666301-46666369   c
            5017-5155           JAXUCZ010000012.1  46665994-46666132   c
            5156-5352           JAXUCZ010000012.1  46665711-46665907   c
            5353-5495           JAXUCZ010000012.1  46665467-46665609   c
            5496-5613           JAXUCZ010000012.1  46664142-46664259   c
            5614-5829           JAXUCZ010000012.1  46663819-46664034   c
            5830-5994           JAXUCZ010000012.1  46662163-46662327   c
            5995-6156           JAXUCZ010000012.1  46661420-46661581   c
            6157-6249           JAXUCZ010000012.1  46661078-46661170   c
            6250-6393           JAXUCZ010000012.1  46660666-46660809   c
            6394-6663           JAXUCZ010000012.1  46660293-46660562   c
            6664-6777           JAXUCZ010000012.1  46659894-46660007   c
            6778-7008           JAXUCZ010000012.1  46659292-46659522   c
            7009-7202           JAXUCZ010000012.1  46658489-46658682   c
            7203-7317           JAXUCZ010000012.1  46658261-46658375   c
            7318-7414           JAXUCZ010000012.1  46655624-46655720   c
            7415-7563           JAXUCZ010000012.1  46655204-46655352   c
            7564-7671           JAXUCZ010000012.1  46654724-46654831   c
            7672-7890           JAXUCZ010000012.1  46653340-46653558   c
            7891-8538           JAXUCZ010000012.1  46652273-46652920   c
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="12"
                     /map="12q16"
     gene            1..8538
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /note="GCN1 activator of EIF2AK4"
                     /db_xref="GeneID:690632"
                     /db_xref="RGD:1593434"
     CDS             1..8016
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /note="translational activator GCN1; GCN1 general control
                     of amino-acid synthesis 1-like 1; eIF-2-alpha kinase
                     activator GCN1; GCN1 eIF2 alpha kinase activator-like 1;
                     GCN1, eIF2 alpha kinase activator homolog"
                     /codon_start=1
                     /product="stalled ribosome sensor GCN1"
                     /protein_id="NP_001162135.1"
                     /db_xref="GeneID:690632"
                     /db_xref="RGD:1593434"
                     /translation="MAADTQVSETLKRFAVKVTTASVKERREILSELGRCIAGKDLPE
                     GAVKGLCKLFCLTLHRYRDAASRRALQAAIQQLAEAQPEATAKNLLHSLQSSGVGSKA
                     CVPSKSSGSAALLALTWTCLLVRIVFPLKAKRQGDIWNKLVEVQCLLLLEVLGGSHKH
                     AVDGAVKKLTKLWKENPGLVEQYFSAILSLEPNQNYAGMLGLLVQFCTNHKEMDVVNQ
                     HKSTLLEFYVKHILMSKAKPPKYLLDNCAPLLRFMSHSEFKDLILPTIQKSLLRSPEN
                     VIETISSLLASVTLDLSQYALDIVKGLANQLKSNSPRLMDEAVLALRNLSRQCSDSSA
                     TEALTKHLFAILGGSEGKLTIIAQKMSVLSGIGSLSHHGVSGPSGQVLNGCVAELFIP
                     FLQQEVHEGTLVHAVSVLALWCNRFTTEVPKKLTDWFKKVFSLKTSTSAVRHAYLQCM
                     LASFRGDTLLQALDFLPLLMQTVEKAASQSTQVPTVTEGVAAALLVSKLSVADSQAEA
                     KLSNFWQLVVDEKKQIFTSEKFLLLASEEALCTVLHLTERLFLDHAHRLTNSRVQQYY
                     RVLVAVLLSRTWHVRRQAQQTVRKLLSSLGGIKLANGLLEELKTVLNSHKVLPLETLV
                     TDAGEVMETGKTYVPPRVLQEALCVISGVPGLKGDITSTEQLAQEMLIISHHPSLVAV
                     QSGLWPALLTRMKIDPEAFITRHLDQIIPRITTQSPLNQSSMNAMGSLSILSPDRVLP
                     QLISSITASVQNPALCLVTREEFAVMQTPAGELFDKSIIQSAQQDSIKKANMKRENKA
                     YSFKEQIIELELKEEIKKKKGIKEEVQLTSKQKEMLQAQMDKEAQIRRRLQELDGELE
                     AALGLLDAIMTRNPRGLTQYIPVLVDAFLPLLKSPLAAPRVKGPFLSLAACVMPPRLK
                     TLGTLVSHVTLRLLKPECALDQSWCQEELPVAVRRAVSLLHTHTIPSRVGKGEPDAAP
                     LSAPAFSLIFPMLKMVLTEMPYHSEEEEEQMAQILQILTVHAQLRASPDTPPERVDEN
                     GPELLPRVAMLRLLTWVIGTGSPRLQVLASDTLTALCASSSGEDGCAFAEQEEVDVLL
                     AALQSPCASVRETALRGLMELHLVLPAPDTDEKNGLNLLRRLWVVKFDKEEEIRKLAE
                     RLWTTMGLDLQSDLCSLLIDDVIYHEAAVRQAGAEALSQAVARYQRQAAEVMGRLMEI
                     YQEKLYRPPPVLDALGRVISESPPDQWEARCGLALALNKLSQYLDSSQVKPLFQFFVP
                     DALNDRNPDVRKCMLDAALATLNAHGKENVNSLLPVFEEFLKDAPNDASYDAVRQSVV
                     VLMGSLAKHLDKSDPKVKPIVAKLIAALSTPSQQVQESVASCLPPLVPAVKEDAGGMI
                     QRLMQQLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMAALTDAIQDKKNFRRRE
                     GALFAFEMLCTMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSAHG
                     VKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHV
                     KVQKAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQTLLDTKFVHFIDA
                     PSLALIMPIVQRAFQDRSTDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLD
                     PVPEVRTVSAKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAG
                     LGVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFTPYVGPIIPCILK
                     ALADENEFVRDTALRAGQRVISMYAETAIALLLPQLEQGLFDDLWRIRFSSVQLLGDL
                     LFHISGVTGKMTTETASEDDNFGTAQSNKAIITALGVDRRNRVLAGLYMGRSDTQLVV
                     RQASLHVWKIVVSNTPRTLREILPTLFGLLLGFLASTCADKRTIAARTLGDLVRKLGE
                     KILPEIIPILEEGLRSQKSDERQGVCIGLSEIMKSTSRDAVLYFSESLVPTARKALCD
                     PLEEVREAAAKTFEQLHSTIGHQALEDILPFLLKQLDDEEVSEFALDGLKQVMAVKSR
                     VVLPYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLGVILPAVMLALKEKLGTPDEQLE
                     MANCQAVILSVEDDTGHRIIIEDLLEATRSPEVGMRQAAAIILNMYCSRSKADYTSHL
                     RSLVSGLIRLFNDSSPVVLGESWDALNAITKKLDAGNQLALIEEFHKEIRYIGNECRG
                     EHVPGFCLPKKGVTSILPVLREGVLTGSPEQKEEAAKALGLVIRLTSADALRPSVVSI
                     TGPLIRILGDRFSWSVKAALLETLSLLLGKVGIALKPFLPQLQTTFTKALQDSNRGVR
                     LKAADALGKLISIHIKVDPLFTELLNGIRVVEDPGIRDTMLQALRFVIQGAGAKVDAA
                     IRKNIVSLLLGMLGHDEDNTRISSAGCLGELCAFLTEEELNTVLQQCLLADVSGIDWM
                     VRHGRSLALSVAVNVAPSRLCTGKYSNEVQDMVLSNAVADRIPIAVSGIRGMGFLMKY
                     HIETGGGQLPPRLSTLLIKCLQNPSSDIRLVAEKMIWWANKEPRPALEPQAIKPILKA
                     LLDNTKDKNTVVRAYSEQAIVNLLKLRQGEELLQSLSKILDVASLEALNECSRRSLKK
                     LACQADSVEQVDDTILT"
     exon            1..18
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            19..121
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            122..185
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            186..317
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            318..426
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            427..525
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            526..660
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            661..729
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            730..838
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            839..913
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1042
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1093
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1192
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1366
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1519
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1520..1612
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1688
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1848
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1849..2038
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2163
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2164..2342
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2343..2436
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2437..2550
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2551..2749
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2750..2905
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            2906..3087
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3088..3168
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3169..3312
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3313..3440
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3441..3630
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3631..3701
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3702..3867
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            3868..4062
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            4063..4309
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            4310..4431
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            4432..4546
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            4547..4726
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            4727..4947
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            4948..5016
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            5017..5155
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            5156..5352
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            5353..5495
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            5496..5613
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            5614..5829
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            5830..5994
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            5995..6156
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            6157..6249
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            6250..6393
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            6394..6663
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            6664..6777
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            6778..7008
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            7009..7202
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            7203..7317
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            7318..7414
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            7415..7563
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            7564..7671
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            7672..7890
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
     exon            7891..8538
                     /gene="Gcn1"
                     /gene_synonym="Gcn1l1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atggcggcgg acacgcaggt ctccgagaca ctgaagcgct ttgcagtgaa ggtgacaaca
       61 gctagtgtga aggagcggag agagattctc agtgaactgg ggcgatgtat tgctgggaaa
      121 gatctgccag agggagcagt gaaagggctc tgcaagctgt tctgcttgac cctgcatcga
      181 tacagggatg cagcttctcg cagagccctg caggcagcca ttcagcagct ggctgaggcc
      241 cagccagaag ccacagccaa gaacctcctg cactctcttc agtcctcagg ggtcggctcc
      301 aaagcgtgtg ttcccagcaa aagcagcggc tcagccgcct tgctggccct gacctggacc
      361 tgccttcttg tgcggattgt ctttcccctg aaggccaagc ggcaaggaga catctggaac
      421 aagctggtgg aggtgcagtg tctgctgctg ctggaggtgc tgggcggctc ccacaagcat
      481 gctgtggacg gcgctgtgaa gaaactcact aagctatgga aagagaaccc tgggctggtg
      541 gagcagtact tctcagccat ccttagccta gaacccaacc aaaactatgc cggcatgctg
      601 gggctgctcg tgcagttctg tacaaatcac aaagagatgg acgtggtcaa tcagcacaag
      661 agtaccctgc tggagttcta cgtgaagcac attctgatga gcaaagccaa gcctcccaag
      721 tacttgttgg acaactgcgc cccgctgctc cggttcatgt cccactctga gtttaaggac
      781 ctgattctgc ctaccataca gaagtccttg ctgaggagcc cggagaatgt tatagagact
      841 atctccagcc tgctggcttc agtgacactt gatctcagcc agtatgccct ggacatcgtg
      901 aaaggcttgg caaatcagct gaagtccaac agcccccgcc tgatggacga agcggtcctg
      961 gcactgcgta acctgtcccg tcagtgcagt gactcgtccg ccaccgaggc cctgactaag
     1021 catctgtttg caatccttgg aggctccgaa gggaagctga ccatcatagc ccagaagatg
     1081 agcgtccttt cagggattgg aagcctcagt catcatgggg tatcggggcc ttcaggtcaa
     1141 gttctgaatg ggtgtgtggc tgagctgttc atcccgttcc tgcaacagga agtgcacgaa
     1201 gggaccttgg tacatgctgt ctctgtcctg gcgctctggt gcaaccggtt cacgacagaa
     1261 gtgcccaaga agctcactga ctggttcaag aaagttttca gccttaaaac ctccacttct
     1321 gcagtgaggc acgcctacct gcagtgcatg ctggcctcct tccgaggtga cacactcttg
     1381 caggccttgg actttctgcc cttgctcatg cagacagtag agaaggcggc ctcccagagc
     1441 actcaggtcc ccacagtcac cgaaggcgtc gccgcagccc tgttggtctc caagctctct
     1501 gtggccgact cacaagctga ggccaaactg agcaactttt ggcagctggt tgtggatgag
     1561 aagaagcaga ttttcacatc tgagaagttt ctgctcctgg cttcagagga ggccctgtgt
     1621 actgtgctgc atctgaccga gaggcttttc cttgaccacg cccacaggct caccaacagc
     1681 agagtacagc agtactaccg ggtcctggtg gctgtgctct tgagccgcac ctggcatgtg
     1741 cgcaggcagg cccagcagac agtccggaag ctgctgtcct ctcttggggg cattaagcta
     1801 gccaacggcc ttttggagga gctgaagact gtcctcaact ctcacaaggt gctacccttg
     1861 gagactcttg tgacagatgc tggggaggta atggagacag gcaagaccta tgtgcctcct
     1921 cgagttctgc aggaagctct gtgtgtcatc tccggcgttc cggggctcaa gggcgacatc
     1981 accagcaccg agcagctggc ccaggagatg ctgatcatct cccaccaccc gtccttagtg
     2041 gcggtgcagt ctggactctg gccagcactt ctcaccagga tgaagattga ccctgaagcc
     2101 tttatcacca ggcacctgga ccagatcatc cccaggatca ctacacagag ccccctgaac
     2161 cagtcttcca tgaacgccat gggctccctt tccatcctgt ctcctgatcg agtcctccct
     2221 cagctcatca gcagtatcac cgcgtctgtg cagaatcctg cgctgtgcct cgtgactcgg
     2281 gaggagtttg ctgtcatgca gacccctgcc ggagagctgt ttgataaatc catcatccag
     2341 agtgcccagc aggacagcat aaaaaaggcc aacatgaagc gagagaacaa ggcctactct
     2401 tttaaggagc agatcatcga gctggagctg aaggaggaaa taaagaagaa gaaaggcata
     2461 aaagaggagg ttcagctgac gagtaagcag aaggagatgc tgcaggccca gatggacaag
     2521 gaggcgcaaa tccggaggcg gctgcaggag ctggatgggg aactagaagc agcgcttgga
     2581 ctcctagacg ccattatgac caggaaccca cgtggcctga cacagtacat ccctgttctg
     2641 gttgacgcct tcctgccctt gctgaagtct cctctggctg ctcccagggt caaaggcccc
     2701 ttcctgtctt tggctgcctg tgtcatgccc cctagactca agaccttggg caccttggtg
     2761 agtcatgtga ccctgcgcct gctgaagcca gagtgtgctc tggatcagtc ctggtgccaa
     2821 gaagagctgc cggtggctgt caggagggct gtgtcactgc tgcacacaca caccattccc
     2881 agcagggtgg gcaagggcga gccagatgct gcaccgctgt ctgcacccgc cttctccttg
     2941 atcttcccga tgttaaagat ggtgctcact gagatgccct accacagcga ggaggaagag
     3001 gagcagatgg cccagatcct tcagatcctc actgtccacg ctcagctgag ggcttcccct
     3061 gacaccccgc ccgagcgggt ggatgagaat ggcccggagt tgctgccccg agtggccatg
     3121 ctacgtctgc tgacgtgggt gatcggaacg ggctcccctc gcctacaggt tcttgcctca
     3181 gacactctga ccgccctatg tgccagcagc agtggggagg acggctgtgc ctttgcggag
     3241 caggaggagg tggatgtgct gctcgctgcc ttgcagtccc catgtgccag cgtgcgcgag
     3301 actgcgctcc gggggctgat ggagctccac ctggtgttgc cagcccctga cactgatgag
     3361 aagaacggcc tgaacctgct gcggagactc tgggttgtga agtttgacaa ggaggaggag
     3421 atacggaagc tggctgagag gctttggacg accatgggcc tcgatctgca gtcagatctc
     3481 tgctcgttgc tgatcgatga tgtgatctat cacgaggctg ctgtgaggca ggctggggct
     3541 gaagcccttt cgcaggcagt ggcacgatac cagcggcaag cagcagaggt tatgggcagg
     3601 ctcatggaga tctaccagga gaagctctat cggccacccc cagtgctgga tgctttgggc
     3661 cgagtgattt cagaatcccc tccagaccag tgggaagcca ggtgtggcct ggccttggcc
     3721 ctgaacaagc tctcacagta tttggatagc tctcaggtga agccgctctt tcagtttttt
     3781 gtcccggatg ccctcaatga ccgaaaccca gatgtgcgga agtgcatgtt agatgcagcg
     3841 cttgccacac tcaatgccca tgggaaggag aatgtcaact cactgctgcc cgtgtttgag
     3901 gagttcctga aggacgcgcc caatgatgcc agctacgacg ccgtgcgaca gagtgtggtg
     3961 gttctgatgg gctctttggc caagcacctg gacaagagtg accccaaagt gaaacccatt
     4021 gttgccaagc tcattgccgc cctctccaca ccttctcagc aggtccagga gtcagtcgcc
     4081 agctgcttac cgccacttgt gcctgccgtc aaggaggacg ctgggggcat gattcagcgg
     4141 ctcatgcagc agctgctgga gtcagacaag tacgcagagc gcaagggggc cgcgtacggg
     4201 ctggcgggcc tggtgaaagg cctgggcatc ctttccctga agcagcagga aatgatggct
     4261 gcactcaccg atgctatcca ggacaagaag aacttccgaa ggcgagaggg agctctgttt
     4321 gccttcgaga tgctctgcac catgctgggg aagctcttcg agccctatgt ggtccacgtg
     4381 ctgccccatc tattgctgtg ctttggagat ggcaatcagt acgtacgcga ggctgcagat
     4441 gactgtgcca aagctgtgat gagcaatttg agtgctcatg gtgtgaagtt ggtgctcccg
     4501 tccctgttag ctgccctgga ggaggagtcc tggaggacca aagctgggtc agtagagctg
     4561 ctcggggcga tggcgtactg tgcacctaag cagctgtcct cctgcctgcc caacattgtg
     4621 cccaagctga cagaggtgct gacagactcc cacgtcaagg tgcagaaggc tgggcagcaa
     4681 gccctcaggc agatcggctc tgtcatcagg aacccggaga ttctcgccat tgccccggtc
     4741 ctgctggacg ctctgacgga cccatcgagg aagactcaga agtgcctgca gaccctgctg
     4801 gacaccaagt ttgtccactt cattgacgcc ccgtccctgg cactcatcat gcccattgtc
     4861 cagagagcct tccaggatcg ctccacagac acacgcaaga tggccgcgca gattattggc
     4921 aacatgtact ccctgacaga ccagaaggac ttggctcctt acctgcccag tgtgacacct
     4981 ggcctgaaag cctcactctt agaccctgtc cctgaggtgc gcacagtgtc tgcaaaggct
     5041 cttggggcca tggtgaaggg catgggagag tcgtgctttg aggacttgct gccgtggctg
     5101 atggagactc tgacatatga gcagagctct gtggaccgct caggcgctgc ccaagggttg
     5161 gctgaggtaa tggctggctt aggagtagag aagttggaaa aattgatgcc agaaattgtg
     5221 gccactgcca gcaaagtgga catcgcaccc catgtccgag acggctacat catgatgttc
     5281 aactacctgc ctatcacctt tggggacaag tttacccctt atgtggggcc catcatccct
     5341 tgtatcctca aagctctggc tgatgagaat gagttcgttc gtgacaccgc cctccgtgcc
     5401 ggccagcggg tcatctccat gtatgctgag acagccattg ccctgctgct gccccagctg
     5461 gagcaaggcc tctttgatga cctctggaga atccggttca gctctgttca actccttggg
     5521 gacctcctgt tccacatctc tggggtcact gggaaaatga ccacagaaac agcctctgag
     5581 gatgataact ttggaactgc tcagtctaac aaggcaatca tcacggccct gggggtcgat
     5641 cggcggaacc gggtcctggc agggctctat atgggccgct cagacaccca gctggtggtg
     5701 aggcaggcgt cactgcacgt gtggaagatt gtcgtttcca acactccccg caccctgcga
     5761 gagatcctgc ccactctctt tggtctgctg ctgggtttcc tggctagcac gtgtgcagat
     5821 aagagaacga ttgcagccag gacgttggga gatcttgtcc ggaagctggg ggagaagatc
     5881 ctccctgaga tcatccccat cctcgaagaa gggctaaggt ctcagaagag cgatgagagg
     5941 cagggcgtgt gcatcgggct cagcgagatc atgaagtcca ccagccggga tgctgtgctc
     6001 tatttctctg agtcccttgt gcccacggca cggaaggcac tatgtgatcc cctggaggag
     6061 gtccgggagg cggcggccaa aacctttgag cagctgcact ccaccattgg ccaccaggct
     6121 ttggaggaca ttctcccgtt cctgctgaag cagctggatg acgaggaggt gtcagaattc
     6181 gccctggatg gtctgaagca ggtcatggcc gttaagagcc gtgtggtgct gccctatctg
     6241 gtacccaagc tgacgacacc acctgtcaac acccgggtgc tggctttcct ctcatctgtg
     6301 gctggcgatg ctctgacgcg tcaccttggt gtgattctcc cagcagtcat gttagcgctg
     6361 aaggaaaagc ttgggactcc agacgagcag ctggagatgg ccaactgtca ggctgtcatc
     6421 ctctcggtag aggatgacac tgggcaccgg atcatcattg aggatctgct ggaggccact
     6481 cgaagccctg aggtgggcat gaggcaggcc gccgccatca tcctcaacat gtactgctcc
     6541 cgctccaagg ctgactatac cagccacctg cgcagcctgg tctccggctt gatccgcctc
     6601 ttcaatgact ccagccctgt ggttctgggg gagagctggg atgctctcaa cgccatcact
     6661 aagaagctgg atgccggcaa ccagctggcc ctgatcgaag aattccataa ggaaatccgc
     6721 tacataggca acgagtgccg aggggagcat gtgccgggct tctgccttcc aaagaaggga
     6781 gtaacctcca tccttccagt gctacgggaa ggagtcctca caggcagtcc tgagcaaaag
     6841 gaagaagcag ccaaggcctt gggcttggtg atccgcctga cgtcggccga tgccctgagg
     6901 ccctctgtgg tcagcatcac tggccctctg attcgcatcc ttggggaccg gttcagctgg
     6961 agtgtgaagg cggccctgct ggagacactc agcctcttgc tgggcaaggt cgggatcgcc
     7021 ctgaagccct tcctgcccca gcttcagacc accttcacca aagctctgca ggactccaat
     7081 cggggcgtac gcctcaaggc tgcggatgcc ctagggaaac tcatttccat ccacatcaag
     7141 gtggaccccc tttttacaga gctgctcaat ggaatccgtg tggtggagga cccaggtata
     7201 agggacacca tgttgcaggc cctcaggttt gtgattcagg gtgcgggcgc caaagtggat
     7261 gctgccatcc ggaaaaacat tgtctcactc ctgctgggca tgctgggcca cgacgaggac
     7321 aacactcgga tctcttcagc cgggtgcctt ggggagctgt gtgcctttct gactgaggag
     7381 gagctcaaca cggtcctgca gcagtgcttg ctggctgacg tatctggcat tgactggatg
     7441 gttcggcacg gccggagctt ggcgctgtct gtggctgtga acgtggctcc cagcagactc
     7501 tgcacaggca aatacagcaa tgaggttcag gacatggtcc tcagcaacgc tgtggcagac
     7561 aggatcccca ttgccgtgag tgggattcgg ggcatgggct tcctcatgaa gtatcacatc
     7621 gagacaggag ggggacagct gccacccaga ctctccaccc tgctcattaa gtgtctgcag
     7681 aacccgtcca gtgacatcag gctggttgcc gagaagatga tctggtgggc caacaaggag
     7741 ccccggcctg ccctggagcc ccaggccatc aagcccatcc tgaaggctct actcgacaac
     7801 accaaggaca agaacacggt agtgagggcc tacagcgagc aggccatcgt gaatctgctc
     7861 aaactgaggc agggcgagga gctgctgcag tcactctcca agatcctgga tgtggccagc
     7921 ttggaggcgc tgaatgagtg cagccggcgg tcgctgaaga agctggcctg ccaggcggac
     7981 tctgtggagc aggtggatga caccatcctg acgtgacaag ctgggtccgc tacagccaca
     8041 ctgccccacg tctgcgctca cactgttttc atttttgaaa atatgtttat tccaagggga
     8101 gctcaggaga tggtgttccc agaaagtatt ttagtatatc agccaaccag agccaaagcc
     8161 ttaaatccaa cccacacaca actgaaaatc acctcctcca tctcttgccc ttatctctgg
     8221 agaagagaaa gaagcgagct cagcgacctc agtgagcgtg tgagcggccg ctagggggcc
     8281 tgactcactc tgccctgcca gagggatgag ctcaggtccc tcttctgtgc tgggtggcct
     8341 gtgtctgctg ggatctgagt ggggcacagg ctgcagagct ccgcctgacc tgacgctgtg
     8401 gcttgctttt catgtggcct gactggacct gacttgtctg gaggaggtga tgctcacgtc
     8461 tcctcctcag ttccaggtct cgggatgcag gccattggaa ggaggtttaa taaaggcttt
     8521 gatttcatct tgaaagaa
//
