LOCUS       NP_059001                274 aa            linear   ROD 03-APR-2024
DEFINITION  glutamate--cysteine ligase regulatory subunit [Rattus norvegicus].
ACCESSION   NP_059001
VERSION     NP_059001.1
DBSOURCE    REFSEQ: accession NM_017305.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 274)
  AUTHORS   Zhang,S., Li,H., Zhang,L., Li,J., Wang,R. and Wang,M.
  TITLE     Effects of troxerutin on cognitive deficits and glutamate cysteine
            ligase subunits in the hippocampus of streptozotocin-induced type 1
            diabetes mellitus rats
  JOURNAL   Brain Res 1657, 355-360 (2017)
   PUBMED   27998794
  REMARK    GeneRIF: Study determined the effects of troxerutin on cognitive
            deficits and glutamate cysteine ligase subunits (GCLM and GCLC) in
            the hippocampus of streptozotocin-induced type 1 diabetes mellitus
            rat: troxerutin alleviates oxidative stress and promotes learning
            potential in STZ-induced diabetic rats likely through GCL
            mechanisms, especially that of GCLC.
REFERENCE   2  (residues 1 to 274)
  AUTHORS   Jang,H.I., Do,G.M., Lee,H.M., Ok,H.M., Shin,J.H. and Kwon,O.
  TITLE     Schisandra Chinensis Baillon regulates the gene expression of phase
            II antioxidant/detoxifying enzymes in hepatic damage induced rats
  JOURNAL   Nutr Res Pract 8 (3), 272-277 (2014)
   PUBMED   24944771
REFERENCE   3  (residues 1 to 274)
  AUTHORS   Sikalidis,A.K., Mazor,K.M., Lee,J.I., Roman,H.B., Hirschberger,L.L.
            and Stipanuk,M.H.
  TITLE     Upregulation of capacity for glutathione synthesis in response to
            amino acid deprivation: regulation of glutamate-cysteine ligase
            subunits
  JOURNAL   Amino Acids 46 (5), 1285-1296 (2014)
   PUBMED   24557597
  REMARK    GeneRIF: Upregulation of both GCLC and GLCM mRNA levels in response
            to cysteine deprivation was dependent on new protein synthesis.
REFERENCE   4  (residues 1 to 274)
  AUTHORS   Ramani,K., Tomasi,M.L., Yang,H., Ko,K. and Lu,S.C.
  TITLE     Mechanism and significance of changes in glutamate-cysteine ligase
            expression during hepatic fibrogenesis
  JOURNAL   J Biol Chem 287 (43), 36341-36355 (2012)
   PUBMED   22942279
REFERENCE   5  (residues 1 to 274)
  AUTHORS   Yang,L., Chen,J.H., Xu,T., Zhou,A.S. and Yang,H.K.
  TITLE     Rice protein improves oxidative stress by regulating glutathione
            metabolism and attenuating oxidative damage to lipids and proteins
            in rats
  JOURNAL   Life Sci 91 (11-12), 389-394 (2012)
   PUBMED   22906634
REFERENCE   6  (residues 1 to 274)
  AUTHORS   Galloway,D.C., Blake,D.G. and McLellan,L.I.
  TITLE     Regulation of gamma-glutamylcysteine synthetase regulatory subunit
            (GLCLR) gene expression: identification of the major
            transcriptional start site in HT29 cells
  JOURNAL   Biochim Biophys Acta 1446 (1-2), 47-56 (1999)
   PUBMED   10395918
REFERENCE   7  (residues 1 to 274)
  AUTHORS   Tipnis,S.R., Blake,D.G., Shepherd,A.G. and McLellan,L.I.
  TITLE     Overexpression of the regulatory subunit of gamma-glutamylcysteine
            synthetase in HeLa cells increases gamma-glutamylcysteine
            synthetase activity and confers drug resistance
  JOURNAL   Biochem J 337 (Pt 3) (Pt 3), 559-566 (1999)
   PUBMED   9895302
REFERENCE   8  (residues 1 to 274)
  AUTHORS   Tu,Z. and Anders,M.W.
  TITLE     Identification of an important cysteine residue in human
            glutamate-cysteine ligase catalytic subunit by site-directed
            mutagenesis
  JOURNAL   Biochem J 336 (Pt 3) (Pt 3), 675-680 (1998)
   PUBMED   9841880
REFERENCE   9  (residues 1 to 274)
  AUTHORS   Misra,I. and Griffith,O.W.
  TITLE     Expression and purification of human gamma-glutamylcysteine
            synthetase
  JOURNAL   Protein Expr Purif 13 (2), 268-276 (1998)
   PUBMED   9675072
REFERENCE   10 (residues 1 to 274)
  AUTHORS   Huang,C.S., Anderson,M.E. and Meister,A.
  TITLE     Amino acid sequence and function of the light subunit of rat kidney
            gamma-glutamylcysteine synthetase
  JOURNAL   J Biol Chem 268 (27), 20578-20583 (1993)
   PUBMED   8104188
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC078867.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC078867.1, L22191.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q42"
     Protein         1..274
                     /product="glutamate--cysteine ligase regulatory subunit"
                     /EC_number="6.3.2.2"
                     /note="GCS light chain; gamma-ECS regulatory subunit;
                     gamma-glutamylcysteine synthetase regulatory subunit;
                     glutamate cysteine ligase (gamma-glutamylcysteine
                     synthetase), regulatory"
                     /calculated_mol_wt=30417
     Site            59
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:22673903; propagated from
                     UniProtKB/Swiss-Prot (P48508.1)"
     Region          <86..>211
                     /region_name="AKR_SF"
                     /note="Aldo-keto reductase (AKR) superfamily; cl00470"
                     /db_xref="CDD:444925"
     Site            263
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P48507; propagated from
                     UniProtKB/Swiss-Prot (P48508.1)"
     CDS             1..274
                     /gene="Gclm"
                     /gene_synonym="Glclr"
                     /coded_by="NM_017305.2:123..947"
                     /db_xref="GeneID:29739"
                     /db_xref="RGD:619871"
ORIGIN      
        1 mgtdsraaga llarastlhl qtgnllnwgr lrkkcpsths eelrdciqkt lnewssqisp
       61 dlvrefpdvl ectmshavek inpdereemk vsaklfivgs nsssstrnav dmacsvlgva
      121 qldsvimasp piedgvnlsl ehlqpyweel enlvqskkiv aigtsdldkt qleqlyqwaq
      181 vkpnsnqvnl asccvmppdl tafakqfdiq llthndpkel lseasfqeal qesipdieaq
      241 ewvplwllry svivksrgii kskgyilqak rkgs
//
