LOCUS       NM_012815               3079 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Rattus norvegicus glutamate-cysteine ligase, catalytic subunit
            (Gclc), mRNA.
ACCESSION   NM_012815
VERSION     NM_012815.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Zhang,S., Li,H., Zhang,L., Li,J., Wang,R. and Wang,M.
  TITLE     Effects of troxerutin on cognitive deficits and glutamate cysteine
            ligase subunits in the hippocampus of streptozotocin-induced type 1
            diabetes mellitus rats
  JOURNAL   Brain Res 1657, 355-360 (2017)
   PUBMED   27998794
  REMARK    GeneRIF: Study determined the effects of troxerutin on cognitive
            deficits and glutamate cysteine ligase subunits (GCLM and GCLC) in
            the hippocampus of streptozotocin-induced type 1 diabetes mellitus
            rat: troxerutin alleviates oxidative stress and promotes learning
            potential in STZ-induced diabetic rats likely through GCL
            mechanisms, especially that of GCLC.
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Mishra,M., Zhong,Q. and Kowluru,R.A.
  TITLE     Epigenetic modifications of Nrf2-mediated glutamate-cysteine
            ligase: implications for the development of diabetic retinopathy
            and the metabolic memory phenomenon associated with its continued
            progression
  JOURNAL   Free Radic Biol Med 75, 129-139 (2014)
   PUBMED   25016074
  REMARK    GeneRIF: in diabetic retinopathy, histone methylation at Gclc-ARE4
            plays an important role in regulating the Nrf2-Gclc-GSH cascade.
REFERENCE   3  (bases 1 to 3079)
  AUTHORS   Jang,H.I., Do,G.M., Lee,H.M., Ok,H.M., Shin,J.H. and Kwon,O.
  TITLE     Schisandra Chinensis Baillon regulates the gene expression of phase
            II antioxidant/detoxifying enzymes in hepatic damage induced rats
  JOURNAL   Nutr Res Pract 8 (3), 272-277 (2014)
   PUBMED   24944771
REFERENCE   4  (bases 1 to 3079)
  AUTHORS   Marson,F.A., Bertuzzo,C.S., Ribeiro,A.F. and Ribeiro,J.D.
  TITLE     Polymorphisms in the glutathione pathway modulate cystic fibrosis
            severity: a cross-sectional study
  JOURNAL   BMC Med Genet 15, 27 (2014)
   PUBMED   24593045
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3079)
  AUTHORS   Hu,G., Zhou,Y., Hong,W., Tian,J., Hu,J., Peng,G., Cui,J., Li,B. and
            Ran,P.
  TITLE     Development and systematic oxidative stress of a rat model of
            chronic bronchitis and emphysema induced by biomass smoke
  JOURNAL   Exp Lung Res 39 (6), 229-240 (2013)
   PUBMED   23682816
  REMARK    GeneRIF: Increased GCLC protein expression in the lungs were
            observed in the rats exposed to biomass smoke and cigarette smoke.
REFERENCE   6  (bases 1 to 3079)
  AUTHORS   Kuo,M.T., Bao,J.J., Curley,S.A., Ikeguchi,M., Johnston,D.A. and
            Ishikawa,T.
  TITLE     Frequent coordinated overexpression of the MRP/GS-X pump and
            gamma-glutamylcysteine synthetase genes in human colorectal cancers
  JOURNAL   Cancer Res 56 (16), 3642-3644 (1996)
   PUBMED   8705999
REFERENCE   7  (bases 1 to 3079)
  AUTHORS   Kondo,T., Yoshida,K., Urata,Y., Goto,S., Gasa,S. and Taniguchi,N.
  TITLE     gamma-Glutamylcysteine synthetase and active transport of
            glutathione S-conjugate are responsive to heat shock in K562
            erythroid cells
  JOURNAL   J Biol Chem 268 (27), 20366-20372 (1993)
   PUBMED   8104187
REFERENCE   8  (bases 1 to 3079)
  AUTHORS   Yan,N. and Meister,A.
  TITLE     Amino acid sequence of rat kidney gamma-glutamylcysteine synthetase
  JOURNAL   J Biol Chem 265 (3), 1588-1593 (1990)
   PUBMED   1967255
REFERENCE   9  (bases 1 to 3079)
  AUTHORS   Beutler,E., Moroose,R., Kramer,L., Gelbart,T. and Forman,L.
  TITLE     Gamma-glutamylcysteine synthetase deficiency and hemolytic anemia
  JOURNAL   Blood 75 (1), 271-273 (1990)
   PUBMED   2294991
REFERENCE   10 (bases 1 to 3079)
  AUTHORS   Lebo,R.V. and Kredich,N.M.
  TITLE     Inactivation of human gamma-glutamylcysteine synthetase by
            cystamine. Demonstration and quantification of enzyme-ligand
            complexes
  JOURNAL   J Biol Chem 253 (8), 2615-2623 (1978)
   PUBMED   24639
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC081702.1.
            
            On Sep 16, 2004 this sequence version replaced NM_012815.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081702.1, J05181.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132263
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q31"
     gene            1..3079
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /note="glutamate-cysteine ligase, catalytic subunit"
                     /db_xref="GeneID:25283"
                     /db_xref="RGD:619868"
     exon            1..213
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..1977
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /EC_number="6.3.2.2"
                     /note="gamma-ECS; GCS heavy chain; gamma-glutamylcysteine
                     synthetase; Glutamylcysteine gamma synthetase light chain"
                     /codon_start=1
                     /product="glutamate--cysteine ligase catalytic subunit"
                     /protein_id="NP_036947.1"
                     /db_xref="GeneID:25283"
                     /db_xref="RGD:619868"
                     /translation="MGLLSQGSPLSWEETQRHADHVRRHGILQFLHIYHAVKDRHKDV
                     LKWGDEVEYMLVSFDHENRKVQLLLNGGDVLETLQEKGERTNPNHPTLWRPEYGSYMI
                     EGTPGQPYGGTMSEFNTVEDNMRKRRKEATSVLGEHQALCTITSFPRLGCPGFTLPEH
                     RPNPEEGGASKSLFFPDEAINKHPRFGTLTRNIRHRRGEKVVINVPIFKDKNTPSPFV
                     ETFPEDEEASKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIV
                     MALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNRFKISKSRYDSIDS
                     YLSKCGEKYNDIDLTIDTEIYEQLLEEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDA
                     NESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTR
                     VILSYKLDFLIPLSKVDENMKVAQERDAVLQGMFYFRKDICKGGNAVVDGCSKAQTSS
                     EPSAEEYTLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCSILNYLKLIKKRA
                     SGELMTVARWMREFIANHPDYKQDSVITDEINYSLILKCNQIANELCECPELLGSGFR
                     KAKYSGGKSDPSD"
     exon            214..326
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            327..509
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            510..623
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            624..682
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            683..816
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            817..891
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1008
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1147
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1260
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1353
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1458
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1540
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1644
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1765
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..3048
                     /gene="Gclc"
                     /gene_synonym="Glclc"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgcccgccgc gcggcccgag ggcgccccca gagcggagct gcggccagga ggaggacgcg
       61 gccatggggc tgctgtccca aggctcgcca ctgagctggg aagagaccca gcgccacgcc
      121 gaccacgtgc ggagacacgg catcctccag ttcctgcaca tctaccacgc agtcaaggac
      181 cggcacaagg acgtgctcaa gtggggtgac gaggtggagt acatgttggt gtcctttgat
      241 catgaaaata ggaaagtcca gttgttactg aatggcggcg atgttcttga aactctgcaa
      301 gagaaggggg agaggacaaa ccccaaccac ccaaccctct ggagaccaga gtatgggagt
      361 tacatgattg aagggacacc tggccagccg tacggaggaa cgatgtccga gttcaacaca
      421 gtggaggaca acatgaggaa acgccggaag gaggctactt ctgtattagg agaacatcag
      481 gctctttgca cgataacttc atttcccagg ctaggctgcc ctggattcac actgccagag
      541 cacagaccca acccagagga aggaggtgca tctaagtccc tcttctttcc agacgaagcc
      601 ataaacaagc acccccgctt tggtactcta acaagaaaca tccggcatcg gagaggagaa
      661 aaggttgtca tcaatgtgcc aatattcaag gacaagaaca caccatctcc gtttgtagaa
      721 acatttcctg aggatgagga ggcatcaaag gcctctaagc cagaccacat ctacatggat
      781 gccatgggat ttgggatggg caactgctgt cttcaggtga cattccaagc ctgcagtata
      841 tctgaggcaa gataccttta tgaccagttg gccactatct gcccaattgt tatggctttg
      901 agtgctgcat cgccatttta ccgaggctac gtgtcagaca ttgattgtcg ctggggagtg
      961 atttctgcat ctgtagatga tagaacacgg gaggagagag gactggagcc cctgaagaac
     1021 aatcgcttta aaatcagtaa gtctcggtat gactcaatag atagctacct gtccaagtgt
     1081 ggagagaagt acaatgacat cgacctgacc atcgacacgg agatctacga gcagctctta
     1141 gaggaaggca tcgatcacct tctggcacag cacgttgctc atctctttat tagagaccca
     1201 ctgacccttt ttgaagagaa aattcatctg gatgatgcca acgagtctga ccattttgag
     1261 aatattcagt ccacaaactg gcagacaatg aggtttaagc ctcctcctcc aaactcagat
     1321 attggatgga gagtagagtt ccgaccaatg gaggtacagt tgacagactt tgagaactct
     1381 gcctatgtgg tatttgtggt actgctgacc agggtgatcc tctcatacaa actagacttc
     1441 ctcattccac tgtccaaggt tgacgagaac atgaaagtgg cacaggagcg agatgccgtc
     1501 ttacagggga tgttttattt caggaaagac atttgcaaag gtggcaacgc cgtggtggat
     1561 gggtgtagca aggcccagac cagctccgag ccatctgcag aggagtacac gctcatgagc
     1621 atagacacca tcatcaatgg gaaggaaggc gtgtttcctg gactcatccc cattctgaac
     1681 tcctaccttg aaaacatgga agtcgacgtg gacacccgat gcagtattct gaactacctg
     1741 aagctaatta agaagagagc atctggagaa ctaatgactg ttgccaggtg gatgagagag
     1801 tttattgcaa accatcctga ctacaagcaa gacagtgtaa taactgatga gatcaactat
     1861 agcctcattt tgaaatgcaa tcaaattgca aatgaattgt gtgaatgtcc agagttactt
     1921 ggatcaggct ttagaaaagc gaagtacagt ggaggtaaaa gcgacccttc agactagaca
     1981 tccagcaaag tggatggcag ctgcagccct gaccccgagc ccactcagac gctgtgaaga
     2041 ccaagatggc ctacagtgtt ccagttgccc acaagtgact agaagcttcc ttcagtaggt
     2101 gaatctgagt ttgtatatgt atatactaaa gtatttttat gattattaac aatgtatttt
     2161 aataacactg tatctacagt catggtgaac tggcttgtac atttttgaat tctgactctg
     2221 gagcgactgc tgtctaggca gttttgtaaa tgtaattgta caatacatgt acctgcattc
     2281 cagagtttca atgtttactg taaaccttct ttaatactac ctgggacctg attcactgaa
     2341 atttattttt aaaaaaatgc tttctcctac ctgtttcttg agtctcctct tgctgtgtac
     2401 gtgaagtcac tggactgcac tgatgccctc tgagggcgag gcaccttacc tgtgacactt
     2461 cctttgcaga gcagtcccac tcctgccacg gaactgaact ctgccagcta ttagaaaaga
     2521 aactacaaat cctagattcg tctgaatata cagactcaga gaatatttag tttcatctga
     2581 aaaggaaaac cttttccttc tatgtacacc aagagctctg tcaaatgtct gctctccatt
     2641 accacctgtc tgacctctgc tgagaacagt cgtcagtgca gccaccaggt ttccgccctc
     2701 tctcaagttt gctgtagcag attggcagac tgtcttctaa cagccattca tttcattcca
     2761 gattttccca ggggaatagt ctgcatcctg cttgctttct gtataaaact tacaaatcaa
     2821 tcatgaaatg cactaatttt tgtgaatcag gaccctaagt gtttaattgt aaataatttg
     2881 tttcatagat tgctttaggt ttgtgttatc tttttaattg ttaactgaat ttaactactt
     2941 ctaaagtaac tgaactgtag attaagccaa gctttacaga taaaaatatt tactgtaatg
     3001 ctaattttga aagattgcaa taaagccact tcagcttaaa gctttaaaaa aaaaaaaaaa
     3061 aaaaaaaaaa aaaaaaaaa
//
