LOCUS       NM_001395627            2069 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus glucosylceramidase beta 3 (Gba3), mRNA.
ACCESSION   NM_001395627
VERSION     NM_001395627.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Marques,A.R., Mirzaian,M., Akiyama,H., Wisse,P., Ferraz,M.J.,
            Gaspar,P., Ghauharali-van der Vlugt,K., Meijer,R., Giraldo,P.,
            Alfonso,P., Irun,P., Dahl,M., Karlsson,S., Pavlova,E.V., Cox,T.M.,
            Scheij,S., Verhoek,M., Ottenhoff,R., van Roomen,C.P., Pannu,N.S.,
            van Eijk,M., Dekker,N., Boot,R.G., Overkleeft,H.S., Blommaart,E.,
            Hirabayashi,Y. and Aerts,J.M.
  TITLE     Glucosylated cholesterol in mammalian cells and tissues: formation
            and degradation by multiple cellular beta-glucosidases
  JOURNAL   J Lipid Res 57 (3), 451-463 (2016)
   PUBMED   26724485
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Wang,L., Seino,J., Tomotake,H., Funakoshi,Y., Hirayama,H. and
            Suzuki,T.
  TITLE     Co-Expression of NEU2 and GBA3 Causes a Drastic Reduction in
            Cytosolic Sialyl Free N-glycans in Human MKN45 Stomach Cancer
            Cells-Evidence for the Physical Interaction of NEU2 and GBA3
  JOURNAL   Biomolecules 5 (3), 1499-1514 (2015)
   PUBMED   26193330
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2069)
  AUTHORS   Dekker,N., Voorn-Brouwer,T., Verhoek,M., Wennekes,T., Narayan,R.S.,
            Speijer,D., Hollak,C.E., Overkleeft,H.S., Boot,R.G. and Aerts,J.M.
  TITLE     The cytosolic beta-glucosidase GBA3 does not influence type 1
            Gaucher disease manifestation
  JOURNAL   Blood Cells Mol Dis 46 (1), 19-26 (2011)
   PUBMED   20728381
REFERENCE   4  (bases 1 to 2069)
  AUTHORS   Hayashi,Y., Okino,N., Kakuta,Y., Shikanai,T., Tani,M., Narimatsu,H.
            and Ito,M.
  TITLE     Klotho-related protein is a novel cytosolic neutral
            beta-glycosylceramidase
  JOURNAL   J Biol Chem 282 (42), 30889-30900 (2007)
   PUBMED   17595169
REFERENCE   5  (bases 1 to 2069)
  AUTHORS   Berrin,J.G., McLauchlan,W.R., Needs,P., Williamson,G.,
            Puigserver,A., Kroon,P.A. and Juge,N.
  TITLE     Functional expression of human liver cytosolic beta-glucosidase in
            Pichia pastoris. Insights into its role in the metabolism of
            dietary glucosides
  JOURNAL   Eur J Biochem 269 (1), 249-258 (2002)
   PUBMED   11784319
REFERENCE   6  (bases 1 to 2069)
  AUTHORS   de Graaf,M., van Veen,I.C., van der Meulen-Muileman,I.H.,
            Gerritsen,W.R., Pinedo,H.M. and Haisma,H.J.
  TITLE     Cloning and characterization of human liver cytosolic
            beta-glycosidase
  JOURNAL   Biochem J 356 (Pt 3), 907-910 (2001)
   PUBMED   11389701
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000014.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643287.30405.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760434 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-75                JAXUCZ010000014.1  64933969-64934043   c
            76-303              JAXUCZ010000014.1  64887405-64887632   c
            304-1100            JAXUCZ010000014.1  64874797-64875593   c
            1101-1224           JAXUCZ010000014.1  64873957-64874080   c
            1225-2069           JAXUCZ010000014.1  64787601-64788445   c
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14q11"
     gene            1..2069
                     /gene="Gba3"
                     /note="glucosylceramidase beta 3"
                     /db_xref="GeneID:289687"
                     /db_xref="RGD:1309539"
     exon            1..75
                     /gene="Gba3"
                     /inference="alignment:Splign:2.1.0"
     CDS             18..1427
                     /gene="Gba3"
                     /EC_number="3.2.1.21"
                     /note="cytosolic beta-glucosidase; glucosidase, beta, acid
                     3 (cytosolic)"
                     /codon_start=1
                     /product="glucosylceramidase beta 3"
                     /protein_id="NP_001382556.1"
                     /db_xref="GeneID:289687"
                     /db_xref="RGD:1309539"
                     /translation="MAFPVGFGWGAATAAYQVEGGWDADGRGPCVWDTFTHQGGERVF
                     ENQTGDVACGSYTLWEEDLKCIKQLGLTHYRFSLSWSRLLPDGTTGFINQKGIDYYNK
                     IIDDLLKNGVTPIVAIYHFDLPQALEDLGGWLSEAIVEAFDKYAQFCFSTFGDRVKQW
                     LTINEPNILALLAYDMGIFAPGVPHIGIGGYQAAHNLIKAHARSWHSYDSLFREEQKG
                     FVSLSLFFCWLEPADPNSEIDQEATKRAINFHLDFFAKPIFIDGDYPDVVKSQVASMS
                     KKQGYPSSRLPEFTEEEKKMIKGTADFFAVQYYTTRLVRHQENKKRELGFLQDVEIEF
                     FPNPFWKNVGWIYVVPWGIRKLLKYIKDTYNNPVIYITENGFPQCDPPSLDDTQRWEY
                     FRQTFQELFKAIHVDDVNLQLYCAWSLLDNFEWNNGYSRRFGLFHVDFEDPARPRTPY
                     RSAKEYAKVIRDNGLAGAM"
     exon            76..303
                     /gene="Gba3"
                     /inference="alignment:Splign:2.1.0"
     exon            304..1100
                     /gene="Gba3"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1224
                     /gene="Gba3"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..2069
                     /gene="Gba3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 attctacctc tgcagtcatg gctttccctg tgggattcgg atggggagca gccacggctg
       61 cataccaagt agaaggaggc tgggatgcag atggaagagg cccttgtgtc tgggacacat
      121 tcactcatca gggaggagag cgagtttttg agaaccagac tggtgatgta gcttgtggca
      181 gctacactct gtgggaagaa gatttgaaat gtatcaaaca gcttggattg actcattacc
      241 gtttctctct ttcctggtca cgtctgttac ctgatgggac gacaggtttc atcaaccaga
      301 aaggaatcga ctattacaac aagatcatag acgatttgtt aaagaatggt gtgacgccca
      361 tagtggccat ctatcacttt gatttgcctc aagctttgga agacctagga ggttggttgt
      421 cagaagcaat tgtagaagcc tttgacaaat atgctcagtt ttgcttcagt acctttggag
      481 accgagtgaa gcagtggctc accataaatg agcctaatat tcttgctctt ttggcctatg
      541 acatgggtat ctttgctcct ggtgtgcctc atattgggat tgggggttat caagcagctc
      601 ataatttgat taaggctcat gccagatcct ggcacagcta tgattcttta ttccgagaag
      661 aacagaaggg ttttgtgtcc ctatcattat ttttttgctg gctagaacca gcagatccca
      721 actcagagat tgaccaggaa gctactaaga gggccatcaa tttccacttg gatttctttg
      781 ctaaacccat atttattgat ggtgattatc cagatgttgt caagtcccag gttgcctcca
      841 tgagcaaaaa gcaaggctat ccatcatcaa ggcttccaga attcacagag gaagagaaga
      901 aaatgatcaa aggcactgcc gatttctttg ctgttcaata ttatacaact cgcttagtca
      961 ggcatcagga gaataagaag agagaactgg gcttccttca ggatgtggag attgaatttt
     1021 ttcctaatcc attttggaaa aatgtgggtt ggatctacgt ggtaccatgg ggaatacgaa
     1081 aactactgaa atatattaag gatacatata ataaccctgt aatttacatc accgagaatg
     1141 ggtttcccca gtgtgacccc ccatctcttg atgacactca gcgttgggag tatttcagac
     1201 agacatttca ggaactattc aaagctatcc atgttgatga tgtcaatctc caactctact
     1261 gtgcgtggtc gcttctggat aactttgagt ggaacaatgg atacagcaga cggtttggtc
     1321 tcttccacgt tgattttgaa gatcccgcta gaccccggac tccatacaga tcagccaagg
     1381 aatatgccaa ggtcatcaga gacaatggcc tggcaggcgc tatgtaggga aggcagttgg
     1441 gaaatgccct gctggaagga ctgagggacg cattggtcat gtctctcaag ttgcctctgt
     1501 ctcaatggtc tgtgattata aagcctgaaa cctaagacta tatctgctga gaagctctac
     1561 ttggctaacg gcattttatc acacagcatc ttagatagtg aaatagagaa tcacagaaat
     1621 taagttttat taaaaatttt ggtttgattt aaaaacttca atttaaattc ttagaataat
     1681 ctcctagaaa aggaacagtg agtttataaa gataatatac tgtttatttg taactctaac
     1741 agcagaaacc ttagaagggt agattattgt tacaaaggga gatttatgca cctccttact
     1801 ttggctacat agctattttt gctttggaga agatctctgt tagtgtttga tccataaaat
     1861 ttccagtatt taatctagca cagaatagcc aagctaaaag ccacaacata cgttctatac
     1921 aacagaggaa atgataatat atttaatgaa atatatatgt taatatgttc atgatatgtt
     1981 ttgtcaatga ttggttaaat atgtctttct tttgtctaat gaaatttgga aaatgttaaa
     2041 aaataaaatg aattacgttt tgaatggaa
//
