LOCUS       NP_112293                423 aa            linear   ROD 14-FEB-2025
DEFINITION  glycine amidinotransferase, mitochondrial precursor [Rattus
            norvegicus].
ACCESSION   NP_112293 XP_063140701 XP_342509
VERSION     NP_112293.1
DBSOURCE    REFSEQ: accession NM_031031.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 423)
  AUTHORS   Lai,Q., Yuan,G., Wang,H., Liu,Z., Kou,J., Yu,B. and Li,F.
  TITLE     Metabolomic profiling of metoprolol-induced cardioprotection in a
            murine model of acute myocardial ischemia
  JOURNAL   Biomed Pharmacother 124, 109820 (2020)
   PUBMED   31972362
REFERENCE   2  (residues 1 to 423)
  AUTHORS   Ellery,S.J., Murthi,P., Gatta,P.A.D., May,A.K., Davies-Tuck,M.L.,
            Kowalski,G.M., Callahan,D.L., Bruce,C.R., Wallace,E.M.,
            Walker,D.W., Dickinson,H. and Snow,R.J.
  TITLE     The Effects of Early-Onset Pre-Eclampsia on Placental Creatine
            Metabolism in the Third Trimester
  JOURNAL   Int J Mol Sci 21 (3), 806 (2020)
   PUBMED   31991880
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 423)
  AUTHORS   Kazak,L., Chouchani,E.T., Lu,G.Z., Jedrychowski,M.P., Bare,C.J.,
            Mina,A.I., Kumari,M., Zhang,S., Vuckovic,I., Laznik-Bogoslavski,D.,
            Dzeja,P., Banks,A.S., Rosen,E.D. and Spiegelman,B.M.
  TITLE     Genetic Depletion of Adipocyte Creatine Metabolism Inhibits
            Diet-Induced Thermogenesis and Drives Obesity
  JOURNAL   Cell Metab 26 (4), 660-671 (2017)
   PUBMED   28844881
  REMARK    Erratum:[Cell Metab. 2017 Oct 3;26(4):693. doi:
            10.1016/j.cmet.2017.09.007.. PMID: 28978428]
REFERENCE   4  (residues 1 to 423)
  AUTHORS   DesRoches,C.L., Bruun,T., Wang,P., Marshall,C.R. and
            Mercimek-Mahmutoglu,S.
  TITLE     Arginine-Glycine Amidinotransferase Deficiency and Functional
            Characterization of Missense Variants in GATM
  JOURNAL   Hum Mutat 37 (9), 926-932 (2016)
   PUBMED   27233232
REFERENCE   5  (residues 1 to 423)
  AUTHORS   Stockler-Ipsiroglu,S., Apatean,D., Battini,R., DeBrosse,S.,
            Dessoffy,K., Edvardson,S., Eichler,F., Johnston,K., Koeller,D.M.,
            Nouioua,S., Tazir,M., Verma,A., Dowling,M.D., Wierenga,K.J.,
            Wierenga,A.M., Zhang,V. and Wong,L.J.
  TITLE     Arginine:glycine amidinotransferase (AGAT) deficiency: Clinical
            features and long term outcomes in 16 patients diagnosed worldwide
  JOURNAL   Mol Genet Metab 116 (4), 252-259 (2015)
   PUBMED   26490222
REFERENCE   6  (residues 1 to 423)
  AUTHORS   Tormanen,C.D.
  TITLE     Comparison of the properties of purified mitochondrial and
            cytosolic rat kidney transamidinase
  JOURNAL   Int J Biochem 22 (11), 1243-1250 (1990)
   PUBMED   2257950
REFERENCE   7  (residues 1 to 423)
  AUTHORS   Hirata,M.
  TITLE     [Study of impaired metabolism of guanidinoacetic acid in
            uremia--the compensatory role of the pancreas in guanidinoacetic
            acid synthesis]
  JOURNAL   Nihon Jinzo Gakkai Shi 31 (9), 951-961 (1989)
   PUBMED   2685430
REFERENCE   8  (residues 1 to 423)
  AUTHORS   Kuwagaki,Y. and Sudo,J.
  TITLE     Interrelation of urinary and plasma levels of guanidinoacetic acid
            with alteration in renal activity of glycine amidinotransferase in
            acute renal failure rats
  JOURNAL   Chem Pharm Bull (Tokyo) 37 (3), 781-784 (1989)
   PUBMED   2752493
REFERENCE   9  (residues 1 to 423)
  AUTHORS   Karelin,A.A.
  TITLE     [Creatine biosynthesis enzymes in the postnatal development of
            rats: the role of cyclo-3',5'-AMP and glucagon in the postnatal
            induction of liver guanidine acetate-N-methyltransferase]
  JOURNAL   Ontogenez 9 (2), 154-159 (1978)
   PUBMED   205820
REFERENCE   10 (residues 1 to 423)
  AUTHORS   Magri,E., Baldoni,G. and Grazi,E.
  TITLE     On the biosynthesis of creatine. Intramitochondrial localization of
            transamidinase from rat kidney
  JOURNAL   FEBS Lett 55 (1), 91-93 (1975)
   PUBMED   1140432
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            On or before Apr 3, 2024 this sequence version replaced
            XP_063140701.1, XP_342509.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26360176.475930.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG   :: lack of evidence for use of
                                                  upstream AUG
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Rattus norvegicus"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q35"
     Protein         1..423
                     /product="glycine amidinotransferase, mitochondrial
                     precursor"
                     /EC_number="2.1.4.1"
                     /note="glycine amidinotransferase, mitochondrial;
                     l-arginine:glycine amidinotransferase; transamidinase;
                     L-arginine: glycine amidinotransferase"
                     /calculated_mol_wt=43624
     transit_peptide 1..43
                     /note="Mitochondrion; propagated from UniProtKB/Swiss-Prot
                     (P50442.1)"
                     /calculated_mol_wt=4636
     mat_peptide     44..423
                     /product="Glycine amidinotransferase, mitochondrial.
                     /id=PRO_0000001208"
                     /note="propagated from UniProtKB/Swiss-Prot (P50442.1)"
                     /calculated_mol_wt=43624
     Site            46
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P50440; propagated from
                     UniProtKB/Swiss-Prot (P50442.1)"
     Site            49
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P50440; propagated from
                     UniProtKB/Swiss-Prot (P50442.1)"
     Region          65..415
                     /region_name="amidinotransferase_AGAT-like"
                     /note="L-arginine:glycine amidinotransferase and similar
                     proteins; cd21136"
                     /db_xref="CDD:439148"
     Site            order(167,169..170,256,303,305..306,322,354..355,361,402,
                     406..407)
                     /site_type="other"
                     /note="chemical substrate binding site [chemical binding]"
                     /db_xref="CDD:439148"
     Site            385
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P50440; propagated from
                     UniProtKB/Swiss-Prot (P50442.1)"
     CDS             1..423
                     /gene="Gatm"
                     /gene_synonym="AT"
                     /coded_by="NM_031031.3:675..1946"
                     /db_xref="GeneID:81660"
                     /db_xref="RGD:71090"
ORIGIN      
        1 mlrvrclrgg srgaeavhyi gsrlggsltg wvqrtfqstq aatassqnsc aaedkathpl
       61 pkdcpvssyn ewdpleeviv graenacvpp ftvevkanty ekywpfyqkn gglyfpkdhl
      121 kkavaeveem cnilsmegvt vkrpdpidws lkyktpdfes tglysamprd ilmvvgneii
      181 eapmawrsrf feyrayrsii kdyfhrgakw ttapkptmad elydqdypih svedrhklaa
      241 qgkfvttefe pcfdaadfir agrdifaqrs qvtnylgiew mrrhlapdyr vhiisfkdpn
      301 pmhidatfni igpglvlsnp drpchqidlf kkagwtivtp ptpvipddhp lwmsskwlsm
      361 nvlmldekrv mvdanevpiq kmfeklgist ikvnirnans lgggfhcwtc dvrrrgtlqs
      421 yfd
//
