LOCUS       NM_001271139            2459 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Rattus norvegicus glycyl-tRNA synthetase 1 (Gars1), mRNA.
ACCESSION   NM_001271139 XM_216152
VERSION     NM_001271139.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Oprescu,S.N., Chepa-Lotrea,X., Takase,R., Golas,G., Markello,T.C.,
            Adams,D.R., Toro,C., Gropman,A.L., Hou,Y.M., Malicdan,M.C.V.,
            Gahl,W.A., Tifft,C.J. and Antonellis,A.
  TITLE     Compound heterozygosity for loss-of-function GARS variants results
            in a multisystem developmental syndrome that includes severe growth
            retardation
  JOURNAL   Hum Mutat 38 (10), 1412-1420 (2017)
   PUBMED   28675565
REFERENCE   3  (bases 1 to 2459)
  AUTHORS   He,W., Bai,G., Zhou,H., Wei,N., White,N.M., Lauer,J., Liu,H.,
            Shi,Y., Dumitru,C.D., Lettieri,K., Shubayev,V., Jordanova,A.,
            Guergueltcheva,V., Griffin,P.R., Burgess,R.W., Pfaff,S.L. and
            Yang,X.L.
  TITLE     CMT2D neuropathy is linked to the neomorphic binding activity of
            glycyl-tRNA synthetase
  JOURNAL   Nature 526 (7575), 710-714 (2015)
   PUBMED   26503042
  REMARK    Erratum:[Nature. 2016 Apr 21;532(7599):402. doi:
            10.1038/nature16499.. PMID: 26789244]
REFERENCE   4  (bases 1 to 2459)
  AUTHORS   Guo,R.T., Chong,Y.E., Guo,M. and Yang,X.L.
  TITLE     Crystal structures and biochemical analyses suggest a unique
            mechanism and role for human glycyl-tRNA synthetase in Ap4A
            homeostasis
  JOURNAL   J Biol Chem 284 (42), 28968-28976 (2009)
   PUBMED   19710017
REFERENCE   5  (bases 1 to 2459)
  AUTHORS   Achilli,F., Bros-Facer,V., Williams,H.P., Banks,G.T., AlQatari,M.,
            Chia,R., Tucci,V., Groves,M., Nickols,C.D., Seburn,K.L.,
            Kendall,R., Cader,M.Z., Talbot,K., van Minnen,J., Burgess,R.W.,
            Brandner,S., Martin,J.E., Koltzenburg,M., Greensmith,L., Nolan,P.M.
            and Fisher,E.M.
  TITLE     An ENU-induced mutation in mouse glycyl-tRNA synthetase (GARS)
            causes peripheral sensory and motor phenotypes creating a model of
            Charcot-Marie-Tooth type 2D peripheral neuropathy
  JOURNAL   Dis Model Mech 2 (7-8), 359-373 (2009)
   PUBMED   19470612
REFERENCE   6  (bases 1 to 2459)
  AUTHORS   Nangle,L.A., Zhang,W., Xie,W., Yang,X.L. and Schimmel,P.
  TITLE     Charcot-Marie-Tooth disease-associated mutant tRNA synthetases
            linked to altered dimer interface and neurite distribution defect
  JOURNAL   Proc Natl Acad Sci U S A 104 (27), 11239-11244 (2007)
   PUBMED   17595294
REFERENCE   7  (bases 1 to 2459)
  AUTHORS   Cader,M.Z., Ren,J., James,P.A., Bird,L.E., Talbot,K. and
            Stammers,D.K.
  TITLE     Crystal structure of human wildtype and S581L-mutant glycyl-tRNA
            synthetase, an enzyme underlying distal spinal muscular atrophy
  JOURNAL   FEBS Lett 581 (16), 2959-2964 (2007)
   PUBMED   17544401
REFERENCE   8  (bases 1 to 2459)
  AUTHORS   Xie,W., Nangle,L.A., Zhang,W., Schimmel,P. and Yang,X.L.
  TITLE     Long-range structural effects of a Charcot-Marie-Tooth
            disease-causing mutation in human glycyl-tRNA synthetase
  JOURNAL   Proc Natl Acad Sci U S A 104 (24), 9976-9981 (2007)
   PUBMED   17545306
REFERENCE   9  (bases 1 to 2459)
  AUTHORS   Antonellis,A., Lee-Lin,S.Q., Wasterlain,A., Leo,P., Quezado,M.,
            Goldfarb,L.G., Myung,K., Burgess,S., Fischbeck,K.H. and Green,E.D.
  TITLE     Functional analyses of glycyl-tRNA synthetase mutations suggest a
            key role for tRNA-charging enzymes in peripheral axons
  JOURNAL   J Neurosci 26 (41), 10397-10406 (2006)
   PUBMED   17035524
REFERENCE   10 (bases 1 to 2459)
  AUTHORS   Lang,I.M., Chuang,T.L., Barbas,C.F. 3rd and Schleef,R.R.
  TITLE     Purification of storage granule protein-23. A novel protein
            identified by phage display technology and interaction with type I
            plasminogen activator inhibitor
  JOURNAL   J Biol Chem 271 (47), 30126-30135 (1996)
   PUBMED   8939962
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On Sep 18, 2014 this sequence version replaced NM_001271139.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMEA5760383, SAMEA5760386
                              [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-260               JAXUCZ010000004.1  85501865-85502124
            261-362             JAXUCZ010000004.1  85511957-85512058
            363-465             JAXUCZ010000004.1  85513620-85513722
            466-607             JAXUCZ010000004.1  85515991-85516132
            608-696             JAXUCZ010000004.1  85517952-85518040
            697-773             JAXUCZ010000004.1  85518485-85518561
            774-919             JAXUCZ010000004.1  85518985-85519130
            920-1069            JAXUCZ010000004.1  85524674-85524823
            1070-1232           JAXUCZ010000004.1  85526242-85526404
            1233-1397           JAXUCZ010000004.1  85528718-85528882
            1398-1505           JAXUCZ010000004.1  85531707-85531814
            1506-1651           JAXUCZ010000004.1  85532975-85533120
            1652-1737           JAXUCZ010000004.1  85536771-85536856
            1738-1847           JAXUCZ010000004.1  85538889-85538998
            1848-1941           JAXUCZ010000004.1  85540452-85540545
            1942-2132           JAXUCZ010000004.1  85541128-85541318
            2133-2459           JAXUCZ010000004.1  85542550-85542876
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q24"
     gene            1..2459
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /note="glycyl-tRNA synthetase 1"
                     /db_xref="GeneID:297113"
                     /db_xref="RGD:1307856"
     exon            1..260
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..2258
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /EC_number="6.1.1.14"
                     /note="AP-4-A synthetase; diadenosine tetraphosphate
                     synthetase; ap4A synthetase"
                     /codon_start=1
                     /product="glycine--tRNA ligase"
                     /protein_id="NP_001258068.1"
                     /db_xref="GeneID:297113"
                     /db_xref="RGD:1307856"
                     /translation="MPCLLPTLLRATRAALLLQSPRVVAAPASQRLLSAPAQPAASPS
                     SMDSAEELLAPLRLAVRQQGDFVRKLKEDKAPQVDVDRAVAELKARKRVLEAKELALQ
                     PKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQ
                     EEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDK
                     KCSAEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPTTGNDLSPPVPFNLMFQTFIG
                     PGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVRE
                     FTMAEIEHFVDPTEKDHPKFPSVADLYLYLYSAKAQVTGQSARKMRLGDAVEQGVINN
                     SVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGC
                     ADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPNKGAVGKAYKKDAKLVLEYL
                     GACDECYITEMELLLSEKGEFTIETEGKTFQLTKDMVSVKRFQKTLHVEEVVPSVIEP
                     SFGLGRIMYTILEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELS
                     EALTRNGVSHKVDDSSGSIGRRYARTDEIGVAFGITIDFDTVNKTPHTATLRDRDSMR
                     QIRAEVSELPSVVRDLANGNITWADVEARYPLFEGQETGKKETVEE"
     exon            261..362
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            363..465
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            466..607
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            608..696
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            697..773
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            774..919
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1069
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1232
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1397
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1505
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1651
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1737
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1847
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            1848..1941
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            1942..2132
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
     exon            2133..2459
                     /gene="Gars1"
                     /gene_synonym="GlyRS; RGD1559871"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tttcatcatg caccgggcgg gccgcgcgcc gcttccgtag ccgccctgcg caccgcctag
       61 gcggcgtgct catgccctgt ctgctcccca cgctgctcag agccacccgc gccgcgctgc
      121 tcctacagtc gccccgagtg gtcgcagcgc ccgcgtctca gcggctcctc agcgctcccg
      181 cgcagcccgc cgcctctccg agcagcatgg acagcgccga ggagctgcta gccccattgc
      241 ggctagctgt gcgccagcag ggagactttg tacggaagtt aaaagaagat aaagccccac
      301 aagtggatgt ggacagagca gtagctgagc tcaaggcccg gaagagggtt ctggaagcaa
      361 aggagctggc gttgcagccc aaagatgaca tcgtagatag agcaaaaatg gaggatactt
      421 tgaagaggag atttttctac gatcaagctt ttgctattta tggaggtgtc agtggtttgt
      481 atgactttgg gccagtggga tgtgctttga agaacaatat catccagacc tggaggcagc
      541 actttatcca agaggaacag atcctggaaa ttgactgcac catgctcacc cccgagccag
      601 ttttaaagac ctctggccat gtagacaaat ttgctgactt catggtgaag gacgtgaaga
      661 acggagagtg cttccgcgcg gaccacctgt tgaaagctca tttacagaaa ctgatgtcag
      721 ataagaagtg ctctgctgag aagaaatcag agatggaaag tgtcttggcc cagcttgata
      781 actatggaca acaagaactg gcggatcttt ttgtgaacta taatgtaaaa tctcccacca
      841 ctggcaatga cctgtcccct ccggtacctt ttaacttaat gttccagacc ttcattgggc
      901 ctggaggaaa catgcctgga tatctgagac cagaaactgc acagggaatt ttcctaaatt
      961 tcaaacgact tttggaattc aaccaaggga agttgccttt tgctgctgca cagattggaa
     1021 actcctttag aaatgagatc tcgccccggt ctggactgat ccgagtcagg gaattcacaa
     1081 tggcagagat cgagcacttt gtagatccca ctgagaaaga ccatcccaag ttcccgagcg
     1141 tggccgacct ctacctttat ttgtactctg ccaaagccca ggtcactgga cagtctgctc
     1201 gcaagatgcg tctgggagat gctgttgaac agggtgtgat taacaactca gtattaggct
     1261 attttattgg ccgcatctac ctctacctca cgaaggttgg aatatctcct gataaactcc
     1321 gcttccgaca gcatatggag aatgagatgg cccattatgc ctgcgactgc tgggatgctg
     1381 agtccaaaac gtcctatggc tggatcgaga ttgttggatg tgctgaccga tcctgctatg
     1441 acctctcctg tcatgctcga gccaccaaag tcccgctggt ggctgagaag cccctgaaag
     1501 agcccaaaac agttaacgtt gtacagtttg agcccaacaa gggagccgtg ggcaaggcgt
     1561 acaagaaaga tgcaaagctg gtgctggagt acctcggcgc ctgcgatgag tgctacatta
     1621 ccgagatgga gctgctgctg agcgagaaag gggaattcac tattgaaact gaaggaaaaa
     1681 catttcagtt aacaaaagac atggtcagtg tgaagagatt ccagaaaaca ctacacgtgg
     1741 aagaagttgt tccgagtgta attgagccct cttttggcct gggcaggatc atgtatacca
     1801 tattggaaca tacattccac gtccgggagg gagatgagca gagaacgttc ttcagtttcc
     1861 ctgctgtggt tgctccattc aaatgttctg tccttccact gagccaaaac caagagttta
     1921 tgccatttgt caaggaatta tcggaagctc tgactcgaaa tggcgtgtct cataaagttg
     1981 acgattcctc tgggtctatt ggaaggcgct atgcaagaac tgatgagatt ggcgtggcgt
     2041 ttggcatcac cattgacttt gatacagtga acaagacccc ccacactgca actctgaggg
     2101 accgagactc catgagacag ataagggcag aggtctctga actgcccagt gtggtccgtg
     2161 atctggccaa tggcaacatc acttgggctg atgtggaggc caggtacccc ctctttgaag
     2221 ggcaagagac tggcaagaag gagacagtgg aagaatgaga acagctggcg acttgtgaca
     2281 acctgtgcta ataaataact catcgtgtcc actacacaaa cgtttcgact gcttccaggg
     2341 actatttttc ctcagtggct gcttgatttt acccccacaa ttaaagttga aggaattctg
     2401 aacaaattta caatccatct gttgtttttc cattttttaa caaagacatt tctctcaga
//
