LOCUS       NM_023964               1450 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Rattus norvegicus glyceraldehyde-3-phosphate dehydrogenase,
            spermatogenic (Gapdhs), mRNA.
ACCESSION   NM_023964
VERSION     NM_023964.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Liu,J., Sun,C.M., Zhang,C.L., Wang,X. and Li,J.Y.
  TITLE     Location and characterization of GAPDS in male reproduction
  JOURNAL   Urol Int 90 (4), 449-454 (2013)
   PUBMED   23306140
  REMARK    GeneRIF: GAPDS protein was expressed in both Sertoli cells and
            elongated sperms. The expression of GAPDS gradually increased with
            age in the epididymis.
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   de Mateo,S., Castillo,J., Estanyol,J.M., Ballesca,J.L. and Oliva,R.
  TITLE     Proteomic characterization of the human sperm nucleus
  JOURNAL   Proteomics 11 (13), 2714-2726 (2011)
   PUBMED   21630459
REFERENCE   3  (bases 1 to 1450)
  AUTHORS   Danshina,P.V., Geyer,C.B., Dai,Q., Goulding,E.H., Willis,W.D.,
            Kitto,G.B., McCarrey,J.R., Eddy,E.M. and O'Brien,D.A.
  TITLE     Phosphoglycerate kinase 2 (PGK2) is essential for sperm function
            and male fertility in mice
  JOURNAL   Biol Reprod 82 (1), 136-145 (2010)
   PUBMED   19759366
REFERENCE   4  (bases 1 to 1450)
  AUTHORS   Tanii,I., Yagura,T., Inagaki,N., Nakayama,T., Imaizumi,K. and
            Yoshinaga,K.
  TITLE     Preferential localization of rat GAPDS on the ribs of fibrous
            sheath of sperm flagellum and its expression during flagellar
            formation
  JOURNAL   Acta Histochem Cytochem 40 (1), 19-26 (2007)
   PUBMED   17375205
REFERENCE   5  (bases 1 to 1450)
  AUTHORS   Welch,J.E., Barbee,R.R., Magyar,P.L., Bunch,D.O. and O'Brien,D.A.
  TITLE     Expression of the spermatogenic cell-specific glyceraldehyde
            3-phosphate dehydrogenase (GAPDS) in rat testis
  JOURNAL   Mol Reprod Dev 73 (8), 1052-1060 (2006)
   PUBMED   16700075
  REMARK    GeneRIF: results describe the expression of the spermatogenic
            cell-specific glyceraldehyde 3-phosphate dehydrogenase (GAPDS) in
            rat testis.
REFERENCE   6  (bases 1 to 1450)
  AUTHORS   Eddy,E.M., Toshimori,K. and O'Brien,D.A.
  TITLE     Fibrous sheath of mammalian spermatozoa
  JOURNAL   Microsc Res Tech 61 (1), 103-115 (2003)
   PUBMED   12672126
  REMARK    Review article
REFERENCE   7  (bases 1 to 1450)
  AUTHORS   Welch,J.E., Brown,P.L., O'Brien,D.A., Magyar,P.L., Bunch,D.O.,
            Mori,C. and Eddy,E.M.
  TITLE     Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed
            specifically in spermatogenic cells
  JOURNAL   J Androl 21 (2), 328-338 (2000)
   PUBMED   10714828
REFERENCE   8  (bases 1 to 1450)
  AUTHORS   Bunch,D.O., Welch,J.E., Magyar,P.L., Eddy,E.M. and O'Brien,D.A.
  TITLE     Glyceraldehyde 3-phosphate dehydrogenase-S protein distribution
            during mouse spermatogenesis
  JOURNAL   Biol Reprod 58 (3), 834-841 (1998)
   PUBMED   9510974
REFERENCE   9  (bases 1 to 1450)
  AUTHORS   Ercolani,L., Florence,B., Denaro,M. and Alexander,M.
  TITLE     Isolation and complete sequence of a functional human
            glyceraldehyde-3-phosphate dehydrogenase gene
  JOURNAL   J Biol Chem 263 (30), 15335-15341 (1988)
   PUBMED   3170585
REFERENCE   10 (bases 1 to 1450)
  AUTHORS   Heinz,F. and Freimuller,B.
  TITLE     Glyceraldehyde-3-phosphate dehydrogenase from human tissues
  JOURNAL   Methods Enzymol 89 Pt D, 301-305 (1982)
   PUBMED   7144574
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AJ297631.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ297631.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="W"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q21"
     gene            1..1450
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /note="glyceraldehyde-3-phosphate dehydrogenase,
                     spermatogenic"
                     /db_xref="GeneID:66020"
                     /db_xref="RGD:620150"
     exon            1..106
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /inference="alignment:Splign:2.1.0"
     CDS             4..1302
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /EC_number="1.2.1.12"
                     /function="glycolytic enzyme"
                     /note="spermatogenic glyceraldehyde-3-phosphate
                     dehydrogenase; spermatogenic cell-specific glyceraldehyde
                     3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate
                     dehydrogenase type 2"
                     /codon_start=1
                     /product="glyceraldehyde-3-phosphate dehydrogenase,
                     testis-specific"
                     /protein_id="NP_076454.1"
                     /db_xref="GeneID:66020"
                     /db_xref="RGD:620150"
                     /translation="MSRRDVVLTNVTVVQLRRDPCPCPCPCPCPCPCPVIRPPPPPPK
                     VEEPPPPKEEPPPPPPPPPPPQIEPEEPKEAPPPPPPPPPPPPPPPPPPPKPAKELTV
                     GINGFGRIGRLVLRVCMEKGVRVVAVNDPFIDPEYMVYMFKYDSTHGRYKGTVEHKNG
                     RLVVDNLEINVFQCKEPKEIPWSSVGNPYVVEATGVYLSIEAASGHISSGARRVIVTA
                     PSPDAPMLVMGVNEKDYNPGSMTVVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHA
                     YTATQKTVDGPSKKDWRGGRGAHQNIIPSSTGAAKAVGKVIPELNGKLTGMAFRVPTP
                     NVSVVDLTCRLAQPASYTAIKEAVKAAAKGPMAGILAYTEDQVVSTDFNGDSHSSIFD
                     AKAGIALNDNFVKLVSWYDNEYGYSHRVVDLLRYMFSREK"
     exon            107..320
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            321..417
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            418..524
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            525..615
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            616..734
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            735..816
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            817..968
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1131
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1229
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1420
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1420
                     /gene="Gapdhs"
                     /gene_synonym="gapdh-2; Gapds"
ORIGIN      
        1 gccatgtcga gacgtgacgt ggttcttacc aatgttactg ttgtccagct acggcgggac
       61 ccatgcccat gtccatgccc atgtccatgt ccatgtccat gtcccgtgat cagaccacct
      121 ccacctccac ccaaggttga ggagcctccg ccacccaagg aggagccgcc gccaccgcca
      181 ccaccgcctc ctccacccca gatagagcca gaggagccta aagaggctcc acctccacct
      241 ccacctcctc caccccctcc ccctccccct cctccaccac ccccaaagcc agctaaagag
      301 ctaacagtgg gcatcaatgg atttggacgc attggtcgtc tggtgctacg agtctgcatg
      361 gagaagggcg ttcgggtagt agcagtgaat gacccattca ttgatccaga atacatggtg
      421 tacatgttca aatatgactc cacacatggt agatacaaag gaacggtgga acataagaat
      481 ggacgactag ttgtggacaa ccttgagatc aacgtgttcc agtgcaaaga acctaaggaa
      541 atcccctgga gctctgtagg gaatccctac gtggtggaag ccacaggtgt atacctgtcc
      601 atagaggcgg cttcggggca catttcatct ggtgccaggc gtgtcatcgt cactgcacct
      661 tcccccgatg cacccatgtt ggtcatgggt gtgaatgaga aggactataa ccctggctcc
      721 atgaccgttg tcagcaatgc atcctgtacc accaactgcc tggcccccct cgctaaggtt
      781 attcacgaac gcttcgggat cgtggaaggg ctaatgacca ccgtccatgc ctacacggcc
      841 actcagaaga cagtggacgg gccatcaaag aaggactggc gaggtggccg aggtgcccac
      901 cagaacatca tcccatcttc cactggggct gccaaggctg taggcaaagt catcccagag
      961 ctcaatggga agttaacagg aatggcattc cgggtgccaa ccccaaacgt atcagttgtg
     1021 gacctgacct gccgcctggc tcagcctgct tcctacactg ctatcaagga ggctgtgaaa
     1081 gcagcagcca agggacctat ggctggcatc cttgcttata cagaggacca ggtggtctcc
     1141 acggacttta atggcgattc ccattcttcc atctttgatg ctaaggctgg aattgccctc
     1201 aatgacaact tcgtgaagct tgtttcctgg tacgacaacg aatatggcta cagtcaccgg
     1261 gtagtcgacc tcctccgcta catgtttagc cgagagaaat aaaggcccct ccttgctccc
     1321 ctgcgcacct cgcgtacctg ccttcggctc cccttcaaaa gagggcgcac ttcgcgctgc
     1381 cgccgggtca acaatgaaat aaaaaccaga gtgcgcacag aaaaaaaaaa aaaaaaaaaa
     1441 aaaaaaaaaa
//
