LOCUS       NM_001398784            3859 bp    mRNA    linear   ROD 23-DEC-2021
DEFINITION  Rattus norvegicus glucosidase II alpha subunit (Ganab), transcript
            variant 1, mRNA.
ACCESSION   NM_001398784 XM_006230976
VERSION     NM_001398784.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Tian W, Jiang X, Sung YK, Shuffle E, Wu TH, Kao PN, Tu AB,
            Dorfmuller P, Cao A, Wang L, Peng G, Kim Y, Zhang P, Chappell J,
            Pasupneti S, Dahms P, Maguire P, Chaib H, Zamanian R, Peters-Golden
            M, Snyder MP, Voelkel NF, Humbert M, Rabinovitch M and Nicolls MR.
  TITLE     Phenotypically Silent Bone Morphogenetic Protein Receptor 2
            Mutations Predispose Rats to Inflammation-Induced Pulmonary
            Arterial Hypertension by Enhancing the Risk for Neointimal
            Transformation
  JOURNAL   Circulation 140 (17), 1409-1425 (2019)
   PUBMED   31462075
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Besse,W., Dong,K., Choi,J., Punia,S., Fedeles,S.V., Choi,M.,
            Gallagher,A.R., Huang,E.B., Gulati,A., Knight,J., Mane,S.,
            Tahvanainen,E., Tahvanainen,P., Sanna-Cherchi,S., Lifton,R.P.,
            Watnick,T., Pei,Y.P., Torres,V.E. and Somlo,S.
  TITLE     Isolated polycystic liver disease genes define effectors of
            polycystin-1 function
  JOURNAL   J Clin Invest 127 (5), 1772-1785 (2017)
   PUBMED   28375157
  REMARK    Erratum:[J Clin Invest. 2017 Sep 1;127(9):3558. PMID: 28862642]
REFERENCE   3  (bases 1 to 3859)
  AUTHORS   Caputo AT, Alonzi DS, Marti L, Reca IB, Kiappes JL, Struwe WB,
            Cross A, Basu S, Lowe ED, Darlot B, Santino A, Roversi P and
            Zitzmann N.
  TITLE     Structures of mammalian ER alpha-glucosidase II capture the binding
            modes of broad-spectrum iminosugar antivirals
  JOURNAL   Proc Natl Acad Sci U S A 113 (32), E4630-E4638 (2016)
   PUBMED   27462106
REFERENCE   4  (bases 1 to 3859)
  AUTHORS   Porath B, Gainullin VG, Cornec-Le Gall E, Dillinger EK, Heyer CM,
            Hopp K, Edwards ME, Madsen CD, Mauritz SR, Banks CJ, Baheti S,
            Reddy B, Herrero JI, Banales JM, Hogan MC, Tasic V, Watnick TJ,
            Chapman AB, Vigneau C, Lavainne F, Audrezet MP, Ferec C, Le Meur Y,
            Torres VE and Harris PC.
  CONSRTM   Genkyst Study Group, HALT Progression of Polycystic Kidney Disease
            Group; Consortium for Radiologic Imaging Studies of Polycystic
            Kidney Disease
  TITLE     Mutations in GANAB, Encoding the Glucosidase IIalpha Subunit, Cause
            Autosomal-Dominant Polycystic Kidney and Liver Disease
  JOURNAL   Am J Hum Genet 98 (6), 1193-1207 (2016)
   PUBMED   27259053
REFERENCE   5  (bases 1 to 3859)
  AUTHORS   Zanivan S, Maione F, Hein MY, Hernandez-Fernaud JR, Ostasiewicz P,
            Giraudo E and Mann M.
  TITLE     SILAC-based proteomics of human primary endothelial cell
            morphogenesis unveils tumor angiogenic markers
  JOURNAL   Mol Cell Proteomics 12 (12), 3599-3611 (2013)
   PUBMED   23979707
REFERENCE   6  (bases 1 to 3859)
  AUTHORS   Ghosh D, Lippert D, Krokhin O, Cortens JP and Wilkins JA.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   7  (bases 1 to 3859)
  AUTHORS   Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE and
            Yates JR.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   8  (bases 1 to 3859)
  AUTHORS   Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta
            R, Wang NS and Knepper MA.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   9  (bases 1 to 3859)
  AUTHORS   Pelletier MF, Marcil A, Sevigny G, Jakob CA, Tessier DC, Chevet E,
            Menard R, Bergeron JJ and Thomas DY.
  TITLE     The heterodimeric structure of glucosidase II is required for its
            activity, solubility, and localization in vivo
  JOURNAL   Glycobiology 10 (8), 815-827 (2000)
   PUBMED   10929008
REFERENCE   10 (bases 1 to 3859)
  AUTHORS   Arendt CW and Ostergaard HL.
  TITLE     Identification of the CD45-associated 116-kDa and 80-kDa proteins
            as the alpha- and beta-subunits of alpha-glucosidase II
  JOURNAL   J Biol Chem 272 (20), 13117-13125 (1997)
   PUBMED   9148925
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JACYVU010000045.1.
            
            [WARNING] On Apr 3, 2024 this sequence was replaced by
            NM_001398784.2.
            
            On Dec 23, 2021 this sequence version replaced XM_006230976.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMD00132261,
                              SAMD00132262 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                JACYVU010000045.1  4565967-4566018
            53-157              JACYVU010000045.1  4569942-4570046
            158-266             JACYVU010000045.1  4570197-4570305
            267-394             JACYVU010000045.1  4572337-4572464
            395-574             JACYVU010000045.1  4576115-4576294
            575-640             JACYVU010000045.1  4576768-4576833
            641-710             JACYVU010000045.1  4577612-4577681
            711-798             JACYVU010000045.1  4577849-4577936
            799-895             JACYVU010000045.1  4578020-4578116
            896-1076            JACYVU010000045.1  4578357-4578537
            1077-1230           JACYVU010000045.1  4579318-4579471
            1231-1466           JACYVU010000045.1  4580103-4580338
            1467-1593           JACYVU010000045.1  4580431-4580557
            1594-1673           JACYVU010000045.1  4580671-4580750
            1674-1817           JACYVU010000045.1  4580984-4581127
            1818-1914           JACYVU010000045.1  4581253-4581349
            1915-2016           JACYVU010000045.1  4581858-4581959
            2017-2260           JACYVU010000045.1  4582100-4582343
            2261-2325           JACYVU010000045.1  4583392-4583456
            2326-2402           JACYVU010000045.1  4583618-4583694
            2403-2477           JACYVU010000045.1  4583782-4583856
            2478-2591           JACYVU010000045.1  4584005-4584118
            2592-2704           JACYVU010000045.1  4584237-4584349
            2705-2805           JACYVU010000045.1  4584486-4584586
            2806-3859           JACYVU010000045.1  4584708-4585761
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     gene            1..3859
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /note="glucosidase II alpha subunit"
                     /db_xref="GeneID:293721"
                     /db_xref="RGD:1309775"
     exon            1..52
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..2915
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /EC_number="3.2.1.84"
                     /note="isoform 1 is encoded by transcript variant 1;
                     neutral alpha-glucosidase AB; alpha glucosidase 2 alpha
                     neutral subunit; neutral AB; glucosidase, alpha; neutral
                     AB"
                     /codon_start=1
                     /product="neutral alpha-glucosidase AB isoform 1"
                     /protein_id="NP_001385713.1"
                     /db_xref="GeneID:293721"
                     /db_xref="RGD:1309775"
                     /translation="MAAIAAVAARRRRSWLSLVLAYLGVCLGITLAVDRSNFKTCEES
                     SFCKRQRSIRPGLSPYRALLDTLQLGPDALTVHLTHEVTKVLLVLELQGLQKNMTRIR
                     IDELEPRRPRYRVPDVLVADPATARLSISGRDDNSVELTVAEGPYKIILTAKPFRLDL
                     LEDRSLLLSVNARGLMAFEHQRAPRVPFSDKVSLTLGSMWDKIKNLFSRQEPKDAAEG
                     NGAEPEARPGDGDKPEETQEKAEKDEPGAWEETFKTHSDSKPYGPTSVGLDFSLPGME
                     HVYGIPEHADSLRLKVTEGGEPYRLYNLDVFQYELNNPMALYGSVPVLLAHSFHRDLG
                     IFWLNAAETWVDISSNTAGKTLFGKMLDYLQGSGETPQTDIRWMSESGIVDVFLMLGP
                     SVLDVFRQHASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNMPCDVIWL
                     DIEHADGKRYFTWDPTRFPQPLNMLEHLASKRRKLVAIVDPHIKVDSGYRVHEELRNH
                     GLYVKTRDGSDYEGWCWPGSASYPDFTNPQMRAWWANMFNFHNYEGSAPNLFVWNDMN
                     EPSVFNGPEVTMLKDAVHYGGWEHRDIHNIYGLYVHMATADGLIQRSGGIERPFVLSR
                     AFFSGSQRFGAVWTGDNTAEWDHLKISIPMCLSLALVGLSFCGADVGGFFKNPEPELL
                     VRWYQMGAYQPFFRAHAHLDTGRREPWLLASQYQDAIRDALFQRYSLLPFWYTLFYQA
                     HQEGFPVMRPLWVQYPEDMSTFSMEDQFMLGDALLIHPVSDAGAHGVQVYLPGEEEVW
                     YDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSDCMKEDPITLFVAL
                     SAQGTAQGELFLDDGHTFNYQNRHEFLLRRFSFSGNTLVSSSADPKGHLETPVWIERV
                     VIMGAGKPAAVVLQTKGSPESRLSFQHDPETSVLTLRKPGVNVASDWSIHLR"
     exon            53..157
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            158..266
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            267..394
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            395..574
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            575..640
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            641..710
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            711..798
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            799..895
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1076
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1230
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1466
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1593
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            1594..1673
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            1674..1817
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..1914
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..2016
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2260
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            2261..2325
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            2326..2402
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            2403..2477
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            2478..2591
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            2592..2704
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            2705..2805
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..3859
                     /gene="Ganab"
                     /gene_synonym="G2an"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaacccggta caagatggcg gcgatagcgg cagtggcggc gcgtaggagg cggtcttggt
       61 taagtttggt gctggcatac ttaggggtct gtctggggat tactcttgct gtggatagaa
      121 gcaactttaa gacctgtgaa gagagttcct tttgcaaacg gcagcgaagc attcggccag
      181 gcctctctcc ttaccgcgcc ttgctggaca ctctgcagct tggtcctgat gctcttacag
      241 tccatctgac ccatgaagtc accaaggtgt tgcttgtgct ggagctccag ggccttcaga
      301 agaacatgac tcggatcagg atcgatgagc tagagccccg gcggcctcga taccgagtgc
      361 cagatgtttt agtggctgac cccgccacag ctcggctttc gatttctggc cgtgatgaca
      421 acagtgtgga gctaacagtg gctgaaggac cttacaaaat cattttgaca gcaaagccat
      481 tccgccttga cctgctagaa gaccgaagcc tcctgctcag tgtcaatgcc cgtggactta
      541 tggcttttga gcatcagagg gctcccaggg tccctttctc ggataaagtt agtctcacgc
      601 tcggtagcat gtgggataag atcaagaacc ttttctctag gcaagaacca aaagatgcag
      661 ctgaaggcaa cggagccgag cctgaagcaa ggcctgggga tggtgacaag ccagaggaga
      721 cccaggaaaa ggctgagaaa gatgagccag gagcctggga ggagacattc aaaacacact
      781 ctgacagcaa gccttacggc cccacgtcgg taggtttgga cttttctctg ccaggaatgg
      841 aacatgtgta tgggatccct gagcatgcag acagcctgag actgaaggtc actgagggtg
      901 gtgagccgta ccgtctgtac aacttggatg tgttccagta tgagctgaac aaccccatgg
      961 ctttgtatgg gtctgtgcct gtgctcttgg cacacagctt tcatcgggac ttgggtatct
     1021 tctggcttaa tgctgctgag acatgggttg atatatcgtc caacacagct gggaagaccc
     1081 tgtttgggaa gatgctggat tacctgcagg ggtctgggga gactccacag acagacattc
     1141 gttggatgtc cgagagcggc attgttgatg ttttcctcat gctcggccct tcagtccttg
     1201 atgtcttccg gcagcatgct agccttacag ggacccaggc actaccccca ctcttctccc
     1261 tgggctacca ccagagtcgc tggaactacc gggatgaggc tgatgttttg gaagtggatc
     1321 agggttttga tgatcacaac atgccttgtg atgtgatatg gttggacatt gaacatgctg
     1381 atggcaagcg gtacttcact tgggacccca cccgatttcc tcagcccctc aatatgcttg
     1441 agcacttggc ttctaaaagg cggaagctgg tggccatcgt ggatccccac atcaaggtag
     1501 actctggtta ccgagttcat gaagaattgc gaaaccatgg gctgtatgtt aaaactcggg
     1561 atggctctga ttacgagggc tggtgctggc caggttcggc tagttaccct gacttcacta
     1621 acccacagat gagggcctgg tgggctaaca tgttcaactt tcacaactat gagggttcag
     1681 ctcctaatct ttttgtctgg aatgacatga atgaaccgtc tgtgttcaat ggtcctgaag
     1741 tcaccatgct gaaggatgct gtgcattatg gcggctggga gcaccgggac atccataaca
     1801 tctatggctt atatgtgcac atggcaactg ctgacgggct aatacagcgc tctggtggca
     1861 tagagcggcc ctttgtcctg agtagggcct tcttctcagg ctcccagcgc tttggagctg
     1921 tgtggacagg ggacaacact gcagagtggg atcatttgaa gatctctatc cctatgtgtc
     1981 tcagcctggc actggtgggg ctttccttct gtggcgcgga tgtgggtggc ttcttcaaga
     2041 acccagagcc agaactgctt gtgcgctggt accaaatggg tgcctaccag ccattctttc
     2101 gggctcatgc tcacttggac actgggcggc gagagccgtg gctgttagcg tctcaatacc
     2161 aagatgcaat ccgagatgcc ttgttccagc gatactcttt gctgcccttc tggtataccc
     2221 tcttctatca agctcaccag gaagggtttc ctgtcatgag gcccctctgg gtacagtatc
     2281 ctgaggatat gtctaccttc agtatggagg atcagttcat gcttggggac gcactcctta
     2341 ttcaccctgt gtcggatgct ggggcccatg gagtgcaggt ctatttgcct ggcgaagaag
     2401 aggtgtggta tgacattcaa agctatcaga agcatcatgg tccccagacc ttgtatctgc
     2461 cagtgaccct gagcagtata cctgtgttcc agcgtggcgg gacaattgtc cctcgatgga
     2521 tgcgcgtgag gcgttcttca gactgtatga aggaagatcc tatcactctc tttgttgctc
     2581 tcagtgccca gggcacggcc caaggagagc tctttctcga tgatggacac acttttaact
     2641 atcagaatcg ccatgagttc ctgttgcgtc gattctcttt ctctggcaac acacttgtct
     2701 ctagttcagc agaccccaaa ggccaccttg agacacctgt ctggattgag cgagtggtca
     2761 taatgggggc tggaaagcca gcagccgtgg tgctccagac caaaggctct cctgaaagtc
     2821 gcctgtcctt ccagcatgac cctgagacct ctgtgctgac attgcgtaaa cctggcgtca
     2881 atgtggcatc tgactggagt attcatcttc gataacccaa ggaacttgtt ctgatttggg
     2941 aggaagggtg tgatggtatt gagttacctt ttcttttgcc ttggagattg accctaccta
     3001 gatttcacct tctaattcca agacccagat tctgtcaaca tctgggcaag atgacagggc
     3061 tgtcctaagg ctctgaattc ctcttgtgat ctagtttttc catctgtgtg ttaccaagct
     3121 cttcccttca tacccagtgt cctgttgctg taactgagca cagtcatttg tgaagagccg
     3181 aggggccttg ccgttctttc cccttcctta gggtccccac cttttcctct gaccccctgt
     3241 attcatgcct cttgtgtgtt gatgccattt cttggaagaa gatgagggca gtgaactaca
     3301 gctttccttt tcctcttccc tgttccctcc tttccagaat gcccacccct tcatcccttc
     3361 ccctgaagtc cccttctctg ttccactgat acactgggac caccccttac ctggtaaggg
     3421 acaatggatc aagattgagg ttcctagaga atgtcctctt ccttctcatc tcaacctttt
     3481 cttcctgttt ctttccagag ctgctgtgtt tctcccattc ttagagggag aagcttccac
     3541 acaattaaga ggggatggtt attaaacttc ttttgcccct ctggtcccct tgggacactc
     3601 aagatagaga aataagttgt ggtttcactg aatcacggtc acctgtattt attgctggga
     3661 agaaggctga gggtgggggg agatgatcat gtgtacccag agatggcttc aagccctggg
     3721 aagcgagttg agggaggact caggaaccag aagaaatatt tgtgggaatt tttttttttt
     3781 acttcctctg ggttcccaac tgtgacaggt tttgataaaa aggagaaaca ataaaaaata
     3841 ataataccat agataacaa
//
