LOCUS       NM_012793                994 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Rattus norvegicus guanidinoacetate N-methyltransferase (Gamt),
            transcript variant 1, mRNA.
ACCESSION   NM_012793 NM_001207007
VERSION     NM_012793.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Ellery,S.J., Murthi,P., Gatta,P.A.D., May,A.K., Davies-Tuck,M.L.,
            Kowalski,G.M., Callahan,D.L., Bruce,C.R., Wallace,E.M.,
            Walker,D.W., Dickinson,H. and Snow,R.J.
  TITLE     The Effects of Early-Onset Pre-Eclampsia on Placental Creatine
            Metabolism in the Third Trimester
  JOURNAL   Int J Mol Sci 21 (3), 806 (2020)
   PUBMED   31991880
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  AUTHORS   Hanna-El-Daher,L., Beard,E., Henry,H., Tenenbaum,L. and
            Braissant,O.
  TITLE     Mild guanidinoacetate increase under partial guanidinoacetate
            methyltransferase deficiency strongly affects brain cell
            development
  JOURNAL   Neurobiol Dis 79, 14-27 (2015)
   PUBMED   25896543
  REMARK    GeneRIF: 3D organotypic brain cell cultures showed that GAMT
            deficiency with significant residual enzymatic activity does not
            lead to creatine deficiency but generates sufficient
            guanidinoacetate to irreversibly affect developing brain
REFERENCE   3  (bases 1 to 994)
  AUTHORS   Choe,C.U., Atzler,D., Wild,P.S., Carter,A.M., Boger,R.H., Ojeda,F.,
            Simova,O., Stockebrand,M., Lackner,K., Nabuurs,C., Marescau,B.,
            Streichert,T., Muller,C., Luneburg,N., De Deyn,P.P., Benndorf,R.A.,
            Baldus,S., Gerloff,C., Blankenberg,S., Heerschap,A., Grant,P.J.,
            Magnus,T., Zeller,T., Isbrandt,D. and Schwedhelm,E.
  TITLE     Homoarginine levels are regulated by L-arginine:glycine
            amidinotransferase and affect stroke outcome: results from human
            and murine studies
  JOURNAL   Circulation 128 (13), 1451-1461 (2013)
   PUBMED   24004504
REFERENCE   4  (bases 1 to 994)
  AUTHORS   Braissant,O., Henry,H., Villard,A.M., Speer,O., Wallimann,T. and
            Bachmann,C.
  TITLE     Creatine synthesis and transport during rat embryogenesis:
            spatiotemporal expression of AGAT, GAMT and CT1
  JOURNAL   BMC Dev Biol 5, 9 (2005)
   PUBMED   15918910
  REMARK    GeneRIF: results suggest that de novo synthesis of creatine(Cr) by
            AGAT and GAMT, as well as cellular Cr uptake by CT1, are essential
            during embryonic development
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 994)
  AUTHORS   Komoto,J., Yamada,T., Takata,Y., Konishi,K., Ogawa,H., Gomi,T.,
            Fujioka,M. and Takusagawa,F.
  TITLE     Catalytic mechanism of guanidinoacetate methyltransferase: crystal
            structures of guanidinoacetate methyltransferase ternary complexes
  JOURNAL   Biochemistry 43 (45), 14385-14394 (2004)
   PUBMED   15533043
  REMARK    GeneRIF: On the basis of the crystal structure of
            GAMT-S-adenosyl-L-homocysteine (SAH)+ guanidinoacetate (GAA) and
            the model structure of GAMT-S-adenosylmethionine (SAM)+ GAA, a
            possible catalytic mechanism of the methyl transfer reaction of
            GAMT is proposed.
REFERENCE   6  (bases 1 to 994)
  AUTHORS   Stockler,S., Isbrandt,D., Hanefeld,F., Schmidt,B. and von Figura,K.
  TITLE     Guanidinoacetate methyltransferase deficiency: the first inborn
            error of creatine metabolism in man
  JOURNAL   Am J Hum Genet 58 (5), 914-922 (1996)
   PUBMED   8651275
REFERENCE   7  (bases 1 to 994)
  AUTHORS   Kristensen,T., Lopez,R. and Prydz,H.
  TITLE     An estimate of the sequencing error frequency in the DNA sequence
            databases
  JOURNAL   DNA Seq 2 (6), 343-346 (1992)
   PUBMED   1446073
  REMARK    Erratum:[DNA Seq 1993;3(5):337]
REFERENCE   8  (bases 1 to 994)
  AUTHORS   Fujioka,M., Takata,Y. and Gomi,T.
  TITLE     Recombinant rat guanidinoacetate methyltransferase: structure and
            function of the NH2-terminal region as deduced by limited
            proteolysis
  JOURNAL   Arch Biochem Biophys 285 (1), 181-186 (1991)
   PUBMED   1990977
REFERENCE   9  (bases 1 to 994)
  AUTHORS   Ogawa,H. and Fujioka,M.
  TITLE     Nucleotide sequence of the rat guanidinoacetate methyltransferase
            gene
  JOURNAL   Nucleic Acids Res 16 (17), 8715-8716 (1988)
   PUBMED   3419933
REFERENCE   10 (bases 1 to 994)
  AUTHORS   Ogawa,H., Date,T., Gomi,T., Konishi,K., Pitot,H.C., Cantoni,G.L.
            and Fujioka,M.
  TITLE     Molecular cloning, sequence analysis, and expression in Escherichia
            coli of the cDNA for guanidinoacetate methyltransferase from rat
            liver
  JOURNAL   Proc Natl Acad Sci U S A 85 (3), 694-698 (1988)
   PUBMED   3277179
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000007.1.
            
            On Mar 8, 2022 this sequence version replaced NM_012793.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: FQ225896.1, FQ227114.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-248               JAXUCZ010000007.1  10099284-10099531
            249-394             JAXUCZ010000007.1  10100354-10100499
            395-458             JAXUCZ010000007.1  10100605-10100668
            459-526             JAXUCZ010000007.1  10101041-10101108
            527-637             JAXUCZ010000007.1  10101241-10101351
            638-994             JAXUCZ010000007.1  10101727-10102083
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q11"
     gene            1..994
                     /gene="Gamt"
                     /gene_synonym="GMT"
                     /note="guanidinoacetate N-methyltransferase"
                     /db_xref="GeneID:25257"
                     /db_xref="RGD:2659"
     exon            1..248
                     /gene="Gamt"
                     /gene_synonym="GMT"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..778
                     /gene="Gamt"
                     /gene_synonym="GMT"
                     /EC_number="2.1.1.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     guanidinoacetate methyltransferase"
                     /codon_start=1
                     /product="guanidinoacetate N-methyltransferase isoform 1"
                     /protein_id="NP_036925.2"
                     /db_xref="GeneID:25257"
                     /db_xref="RGD:2659"
                     /translation="MSSSAASPLFAPGEDCGPAWRAAPAAYDTSDTHLQILGKPVMER
                     WETPYMHSLAAAAASRGGRVLEVGFGMAIAASRVQQAPIKEHWIIECNDGVFQRLQNW
                     ALKQPHKVVPLKGLWEEVAPTLPDGHFDGILYDTYPLSEETWHTHQFNFIKTHAFRLL
                     KPGGILTYCNLTSWGELMKSKYTDITAMFEETQVPALLEAGFQRENICTEVMALVPPA
                     DCRYYAFPQMITPLVTKH"
     exon            249..394
                     /gene="Gamt"
                     /gene_synonym="GMT"
                     /inference="alignment:Splign:2.1.0"
     exon            395..458
                     /gene="Gamt"
                     /gene_synonym="GMT"
                     /inference="alignment:Splign:2.1.0"
     exon            459..526
                     /gene="Gamt"
                     /gene_synonym="GMT"
                     /inference="alignment:Splign:2.1.0"
     exon            527..637
                     /gene="Gamt"
                     /gene_synonym="GMT"
                     /inference="alignment:Splign:2.1.0"
     exon            638..994
                     /gene="Gamt"
                     /gene_synonym="GMT"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 accccgcctc ctccagcctg gtggttccgc agccgtactc tcctggcctg gtgtgcacag
       61 cctcaccatg agttcttctg cagccagccc gcttttcgcg cctggcgagg actgcggccc
      121 cgcgtggcgc gcggcccccg cggcctatga tacgtctgac acgcacctgc agatcctggg
      181 caagccagta atggagcgtt gggagacccc ctacatgcat tcgctggcgg ctgctgctgc
      241 ctccagaggg ggccgggtcc tggaagtggg ctttgggatg gccattgcag cctccagggt
      301 gcagcaggcc cccataaagg aacactggat tattgaatgc aacgatgggg tcttccagcg
      361 tctacaaaac tgggccctga agcagccaca taaggttgtt cccttgaaag gcctgtggga
      421 ggaggtggca cctacactgc ctgatggtca ctttgatggg attctatacg acacatatcc
      481 actgtctgaa gagacctggc acactcacca gttcaacttt attaagactc atgctttccg
      541 tttgctgaag cctgggggta tcctcactta ctgcaacctc acgtcctggg gggaactcat
      601 gaagtccaag tacacagaca tcactgccat gtttgaggag actcaggtgc ctgcactgct
      661 ggaagctggc ttccagagag aaaacatctg tacagaggtg atggcgctgg tgcccccagc
      721 cgactgccgc tactatgcct tccctcagat gatcacaccc ctggtcacca agcactgagc
      781 ggctggccca gggctacaag gagaatatgt cctcctcagt gcctttgtag ctggagtgtg
      841 ggctccagcc tctccactat ccctgcagtg tgacatccta acctctgcct ggtacggcca
      901 tctccccaga gctcaggagt aaaataaatg ctaccaagac tagtagtggg ttggctggcg
      961 gtgagccgga ggtgaggccc gcatctggtt caga
//
