LOCUS       NM_031030               4453 bp    mRNA    linear   ROD 16-JUN-2024
DEFINITION  Rattus norvegicus cyclin G associated kinase (Gak), mRNA.
ACCESSION   NM_031030
VERSION     NM_031030.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Beilina,A., Rudenko,I.N., Kaganovich,A., Civiero,L., Chau,H.,
            Kalia,S.K., Kalia,L.V., Lobbestael,E., Chia,R., Ndukwe,K., Ding,J.,
            Nalls,M.A., Olszewski,M., Hauser,D.N., Kumaran,R., Lozano,A.M.,
            Baekelandt,V., Greene,L.E., Taymans,J.M., Greggio,E. and
            Cookson,M.R.
  CONSRTM   International Parkinson's Disease Genomics Consortium; North
            American Brain Expression Consortium
  TITLE     Unbiased screen for interactors of leucine-rich repeat kinase 2
            supports a common pathway for sporadic and familial Parkinson
            disease
  JOURNAL   Proc Natl Acad Sci U S A 111 (7), 2626-2631 (2014)
   PUBMED   24510904
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Yim,Y.I., Sun,T., Wu,L.G., Raimondi,A., De Camilli,P., Eisenberg,E.
            and Greene,L.E.
  TITLE     Endocytosis and clathrin-uncoating defects at synapses of auxilin
            knockout mice
  JOURNAL   Proc Natl Acad Sci U S A 107 (9), 4412-4417 (2010)
   PUBMED   20160091
REFERENCE   3  (bases 1 to 4453)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   4  (bases 1 to 4453)
  AUTHORS   Lee,D.W., Zhao,X., Yim,Y.I., Eisenberg,E. and Greene,L.E.
  TITLE     Essential role of cyclin-G-associated kinase (Auxilin-2) in
            developing and mature mice
  JOURNAL   Mol Biol Cell 19 (7), 2766-2776 (2008)
   PUBMED   18434600
REFERENCE   5  (bases 1 to 4453)
  AUTHORS   Zhang,C.X., Engqvist-Goldstein,A.E., Carreno,S., Owen,D.J.,
            Smythe,E. and Drubin,D.G.
  TITLE     Multiple roles for cyclin G-associated kinase in clathrin-mediated
            sorting events
  JOURNAL   Traffic 6 (12), 1103-1113 (2005)
   PUBMED   16262722
  REMARK    GeneRIF: Early stages of clathrin-mediated endocytosis (CME) were
            partially inhibited when GAK expression was knocked down; GAK plays
            a role in clathrin-dependent trafficking from the trans Golgi
            network.
REFERENCE   6  (bases 1 to 4453)
  AUTHORS   Lee,D.W., Zhao,X., Zhang,F., Eisenberg,E. and Greene,L.E.
  TITLE     Depletion of GAK/auxilin 2 inhibits receptor-mediated endocytosis
            and recruitment of both clathrin and clathrin adaptors
  JOURNAL   J Cell Sci 118 (Pt 18), 4311-4321 (2005)
   PUBMED   16155256
REFERENCE   7  (bases 1 to 4453)
  AUTHORS   Kanaoka,Y., Kimura,S.H., Okazaki,I., Ikeda,M. and Nojima,H.
  TITLE     GAK: a cyclin G associated kinase contains a tensin/auxilin-like
            domain
  JOURNAL   FEBS Lett 402 (1), 73-80 (1997)
   PUBMED   9013862
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from D38560.1.
            
            On Aug 31, 2012 this sequence version replaced NM_031030.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D38560.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132266
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4453              D38560.1           2-4454
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="14"
                     /map="14p22"
     gene            1..4453
                     /gene="Gak"
                     /note="cyclin G associated kinase"
                     /db_xref="GeneID:81659"
                     /db_xref="RGD:621589"
     exon            1..249
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..4022
                     /gene="Gak"
                     /EC_number="2.7.11.1"
                     /note="dnaJ homolog subfamily C member 26"
                     /codon_start=1
                     /product="cyclin-G-associated kinase"
                     /protein_id="NP_112292.1"
                     /db_xref="GeneID:81659"
                     /db_xref="RGD:621589"
                     /translation="MSLLQSALDFLAGPGSLGGAAGRDQSDFVGQTVELGELRLRVRR
                     VLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFLKKLSGHPNIVQFC
                     SAASIGKEESDTGQAEFLLLTELCKGQLVEFLRRVECKGPLSCDSILKIFYQTCRAVQ
                     HMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQKRAMVEEEI
                     TRNTTPMYRTPEIVDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKY
                     SIPVNDTRYTVFHDLIRGMLKVNPEERLSIAEVVRQLQEIAAARNVNPKAPITELLEQ
                     NGGYGNSGPSRAQPPSGGPVNSSGVLALAEYDQPYGGFLDILRGGTERLFTNLKDTSS
                     KVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDAKHPGHYAV
                     YNLSPRIYRASKFHNRVTECGWAVRRAPHLHSLYTLCRSMHAWLREDHRNVCVVHCMD
                     GRAASAVAVCAFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYVCDMVAEEPI
                     TPHSKPMLVKSVVMTPVPLFSKQRNGCRPFCEVYVGEERVTTTSQEYDRMKEFKIEDG
                     KAVIPLGITVQGDVLTIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFA
                     KYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSRDVPPWENTSLRGLNPKILFSNREEQ
                     QDILSKFGKPELPRQPGSTAQYDAEAGSPEAEITESDSPQSSSTDTNHFLHTLDWQEE
                     KDPETGVDNTSPKESQSNLIADGDGSEVSDEEEASCPSEERKPGAGEDTPRLAAGTRQ
                     QDLIFDVGMLAAPQEPVQPEEGVDLLGLHSEGDLRPAAPLQASGVQSSNTDLLSSLLE
                     PSDASQVGPPGDLLGGETPLLLASPVSLLGVQSNLQGKVPDTVDPFDQFLLPSSSDTQ
                     PCSKPDLFGEFLNSDSVASSTAFPSTHSAPPPSCSTAFLHLGDLPAEPNKVIASSSHP
                     DLLGGWDTWAETALPGPASMPVPEGTLFSSAGHPAPPGPNPSQTKSQNPDPFADLSDL
                     SSSLQGLPAGLPAGSFVGTSATTHKSNSSWQTTRPTAPGTSWPPQAKPAPRASEQLRS
                     HFSVIGAREERGVRAPSFAQKPKVSENDFEDLLPNQGFSKSDKKGPKTMAEMRKQELA
                     RDTDPFKLKLLDWIEGKERNIRALLSTLHTVLWDGESRWTPVSMADLVTPEQVKKQYR
                     RAVLVVHPDKATGQPYEQSAKMIFMELNDAWSEFENQGSRPLF"
     exon            250..311
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            312..371
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            372..486
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            487..629
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            630..755
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            756..845
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            846..981
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1094
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1179
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1303
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1353
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1502
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            1503..1625
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1759
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            1760..1954
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2072
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            2073..2152
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            2153..2346
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2493
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            2494..2973
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            2974..3144
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            3145..3267
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            3268..3384
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            3385..3597
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            3598..3743
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            3744..3920
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
     exon            3921..4453
                     /gene="Gak"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cgtcacggcg cgggcggaag atggccgggt tggttgcccg cagctgctaa cccggcagtg
       61 cggagctgga gcctggctcc cgctgccgcg aagcggccgt cgccatgtcg ctgctgcagt
      121 ctgcactgga cttcctggcg ggccctggtt ctcttggcgg agctgccggc cgtgaccaga
      181 gtgacttcgt ggggcagact gtggagctgg gcgagctgcg tctgcgggtg cggcgggtcc
      241 tggccgaggg agggtttgca tttgtttatg aagctcaaga tctgggaagt ggcagagagt
      301 atgcattaaa gagattacta tccaatgaag aggaaaagaa cagagccatc attcaggaag
      361 tatgtttctt gaaaaaactt tctggccacc ccaatattgt ccagttctgc tctgcagcat
      421 ccataggaaa agaggaatcg gacactgggc aggctgagtt cctcctgctt acggagctct
      481 gtaaaggaca gctggtggag tttctcagga gagttgaatg taaaggccct ctatcctgcg
      541 acagcattct gaagatcttc taccagacat gcagagcagt gcagcacatg cacaggcaga
      601 aaccacccat catccacagg gatctcaagg ttgaaaactt actgcttagt aaccagggga
      661 ccattaagct gtgtgacttt ggcagtgcca caaccatctc ccattatcct gactacagct
      721 ggagcgccca gaagcgagca atggtggagg aagagatcac gaggaacacc acacccatgt
      781 acagaacgcc agaaattgta gacctgtatt ctaacttccc tattggcgaa aagcaggata
      841 tctgggcact gggctgtatc ttatacctgc tgtgtttccg gcagcatcct tttgaagatg
      901 gagcaaaact tcggatagtc aatgggaagt attccattcc tgtgaatgac actcgttaca
      961 cagtcttcca tgaccttatt cgtggcatgc taaaggtcaa tccagaagag aggctatcca
     1021 ttgctgaagt tgtccgacaa ctgcaggaaa ttgcagcagc ccggaatgtg aaccccaaag
     1081 cccccatcac agaacttctg gagcagaatg gtggctatgg gaactcaggg ccttcccgag
     1141 cacaaccacc ttctgggggc cctgtgaaca gcagtggagt tttggctctg gcagagtatg
     1201 accagcccta tggtgggttt ctcgatatcc tacggggtgg gacagaacgg ctcttcacca
     1261 acctcaagga tacttcctcc aaggtcatcc agtctgtggc taactatgca aagggcgatc
     1321 ttgacatatc ttacatcaca tccaggattg cagtgatgtc gttcccagca gaaggtgtgg
     1381 agtcagcaat caaaaacaat atagaggatg tacgattgtt tctggatgcc aagcatccag
     1441 gacattatgc tgtctacaac ctttctccaa gaatataccg ggcttccaag ttccacaatc
     1501 gggtcactga gtgtggctgg gcagtcaggc gggcaccaca tctccacagt ttgtatactc
     1561 tatgcaggag catgcatgcc tggctccggg aagaccacag gaacgtctgt gttgtacatt
     1621 gcatggatgg gagagctgcg tctgctgtgg cagtctgtgc attcctgtgc ttctgccgtc
     1681 tcttcagcac tgcagaggct gctgtgtaca tgttcagcat gaagcgctgc ccaccaggca
     1741 tttggccatc ccacaaaagg tacattgaat acgtgtgtga catggtggca gaggaaccca
     1801 tcacgcccca cagcaagcca atgctggtga aatctgttgt catgacccct gtgccactgt
     1861 tcagcaagca gaggaatggc tgccgaccat tctgtgaggt ctatgttggg gaggagcgtg
     1921 taaccaccac atcccaggag tatgacagga tgaaggaatt taaaattgag gatggcaagg
     1981 ctgtcatccc cctgggcata acagttcaag gcgatgtcct caccatcatt taccatgcca
     2041 gatccacttt aggagggaga ctgcaggcca agatggcgtc catgaaaatg ttccagattc
     2101 agttccacac tgggttcgtg cctcgaaacg caaccactgt gaaatttgca aagtatgacc
     2161 tggatgcttg tgacattcaa gagaagtacc cagatctgtt ccaggtgaac ctggaagtgg
     2221 aggtggagcc tagagacagg cccagccgag acgttccacc ttgggagaat accagcctaa
     2281 gggggttaaa ccccaagatc ctcttttcca acagggaaga gcaacaggac attctgtcta
     2341 agtttgggaa accagagcta ccccggcagc cgggctccac agctcagtat gatgctgagg
     2401 cagggtctcc agaggctgag atcacagagt cggactcacc acagagcagc agcacggaca
     2461 ccaaccactt tcttcacaca ctggattggc aggaggaaaa agacccagag actggtgtag
     2521 acaatacctc tcctaaggag agtcagtcta acctgattgc agatggagat ggaagtgaag
     2581 tatcagatga agaagaggct tcatgcccta gtgaagagag aaagcctggg gctggagaag
     2641 atacaccaag gctggcagct gggaccagac agcaagactt aatatttgat gtgggcatgc
     2701 tggctgcccc acaggagcct gtacagcctg aagaaggtgt cgatctcctg gggctgcact
     2761 ctgaagggga cttaaggcct gctgctccct tgcaggctag cggggtccag tctagcaaca
     2821 ctgacctgtt gagctccctt cttgaaccat ctgatgcttc tcaagtggga cctcctggtg
     2881 acctgcttgg tggtgagact cctctgctgt tagcaagccc agtttctctt cttggggtgc
     2941 agagcaacct gcaaggaaaa gtccctgaca ctgtggaccc atttgaccag ttcctgctgc
     3001 catccagctc ggacacccag ccctgctcca agcctgatct ctttggagag tttctcaact
     3061 ctgactctgt agcttcctca actgccttcc catcgaccca cagcgcccca cccccatcct
     3121 gcagcactgc cttcctgcac ctgggagatc tgccagcaga gcccaacaag gtgattgctt
     3181 catccagcca cccagatctg ctaggaggat gggatacgtg ggctgagact gctctacctg
     3241 ggccggcctc catgccagta ccagaaggta ctctcttctc ttctgcaggt cacccagccc
     3301 ctccaggccc caaccccagc caaaccaagt ctcagaaccc agacccattt gctgacctca
     3361 gtgatctcag ctctagcctc caaggcttgc ctgctggact tcctgcaggg agcttcgttg
     3421 gcacatcagc caccactcac aaaagcaaca gctcctggca gacaactcgt cccacagccc
     3481 ctggaacctc atggcccccg caggccaagc cagcccccag agcctctgaa caactaaggt
     3541 ctcactttag tgtgattggg gcccgagaag agagaggtgt ccgtgcgccc agctttgccc
     3601 aaaagccaaa ggtctcagaa aatgattttg aagatctgct gcctaatcaa ggcttctcta
     3661 agtctgacaa gaaggggcca aagaccatgg cagagatgcg gaaacaggaa cttgccagag
     3721 atacagaccc attcaaattg aagcttttgg actggattga aggcaaagag aggaatattc
     3781 gtgcactgct gtccactctg cacacagtat tgtgggatgg ggagagccgc tggacacctg
     3841 tgagtatggc tgacctggtg actccagagc aggtgaagaa gcagtaccgc cgtgcagtgc
     3901 tggtagtgca tcctgataag gccacagggc agccatatga acagtctgcc aagatgatct
     3961 tcatggagct gaatgatgca tggtctgagt ttgaaaacca gggctcaagg cccctcttct
     4021 gaggcctgtg atgactatgg ctgcaggagc tgctcatgaa tcctgaatgc tgctggcctg
     4081 actgaggcac aaatgggacc ataacaggtg tggcaggtga tatcgcccca ggggcctgga
     4141 ccatctactc ccaatcatcc ctgcattcat catggacaaa taccattcca tggctgggag
     4201 agaaagcatt cctcagatct gatatttgtt gttttgtttt tctttttctt gtcccagagg
     4261 aaaagtgttg atttatattc cttccacagc tgtcacaatt tgtgatttac ttggtgtctg
     4321 gtggctgtca cctcttgaat ggcacatgtc gctggcactt ctctcctgta atttcttcaa
     4381 atatggggtg catccacacc ccactctgat aagtttgcca atcatctgta cataattaaa
     4441 ctattttccg atg
//
