LOCUS       NM_012563               1965 bp    mRNA    linear   ROD 07-MAY-2024
DEFINITION  Rattus norvegicus glutamate decarboxylase 2 (Gad2), mRNA.
ACCESSION   NM_012563
VERSION     NM_012563.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Anfimova,P.A., Pankrasheva,L.G., Emanuilov,A.I., Moiseev,K.Y. and
            Maslyukov,P.M.
  TITLE     Expression of GAD65/67 and VGLUT2 in Mediobasal Nuclei of Rat
            Hypothalamus during Aging
  JOURNAL   Bull Exp Biol Med 176 (2), 232-234 (2023)
   PUBMED   38194067
  REMARK    GeneRIF: Expression of GAD65/67 and VGLUT2 in Mediobasal Nuclei of
            Rat Hypothalamus during Aging.
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Zhang,S., Hu,S., Dong,W., Huang,S., Jiao,Z., Hu,Z., Dai,S., Yi,Y.,
            Gong,X., Li,K., Wang,H. and Xu,D.
  TITLE     Prenatal dexamethasone exposure induces anxiety- and
            depressive-like behavior of male offspring rats through
            intrauterine programming of the activation of NRG1-ErbB4 signaling
            in hippocampal PV interneurons
  JOURNAL   Cell Biol Toxicol 39 (3), 657-678 (2023)
   PUBMED   34189720
REFERENCE   3  (bases 1 to 1965)
  AUTHORS   Zeng,C., Lei,D., Lu,Y., Huang,Q., Wu,Y., Yang,S. and Wu,Y.
  TITLE     Parvalbumin in the metabolic pathway of glutamate and
            gamma-aminobutyric acid: Influence on expression of GAD65 and GAD67
  JOURNAL   Arch Biochem Biophys 734, 109499 (2023)
   PUBMED   36587827
  REMARK    GeneRIF: Parvalbumin in the metabolic pathway of glutamate and
            gamma-aminobutyric acid: Influence on expression of GAD65 and
            GAD67.
REFERENCE   4  (bases 1 to 1965)
  AUTHORS   Jiang,W., Kakizaki,T., Fujihara,K., Miyata,S., Zhang,Y., Suto,T.,
            Kato,D., Saito,S., Shibasaki,K., Ishizaki,Y., Isoda,K., Yokoo,H.,
            Obinata,H., Hirano,T., Miyasaka,Y., Mashimo,T. and Yanagawa,Y.
  TITLE     Impact of GAD65 and/or GAD67 deficiency on perinatal development in
            rats
  JOURNAL   FASEB J 36 (2), e22123 (2022)
   PUBMED   34972242
  REMARK    GeneRIF: Impact of GAD65 and/or GAD67 deficiency on perinatal
            development in rats.
REFERENCE   5  (bases 1 to 1965)
  AUTHORS   Kakizaki,T., Ohshiro,T., Itakura,M., Konno,K., Watanabe,M.,
            Mushiake,H. and Yanagawa,Y.
  TITLE     Rats deficient in the GAD65 isoform exhibit epilepsy and premature
            lethality
  JOURNAL   FASEB J 35 (2), e21224 (2021)
   PUBMED   33236473
  REMARK    GeneRIF: Rats deficient in the GAD65 isoform exhibit epilepsy and
            premature lethality.
REFERENCE   6  (bases 1 to 1965)
  AUTHORS   Christgau,S., Aanstoot,H.J., Schierbeck,H., Begley,K., Tullin,S.,
            Hejnaes,K. and Baekkeskov,S.
  TITLE     Membrane anchoring of the autoantigen GAD65 to microvesicles in
            pancreatic beta-cells by palmitoylation in the NH2-terminal domain
  JOURNAL   J Cell Biol 118 (2), 309-320 (1992)
   PUBMED   1321158
REFERENCE   7  (bases 1 to 1965)
  AUTHORS   Bu,D.F., Erlander,M.G., Hitz,B.C., Tillakaratne,N.J., Kaufman,D.L.,
            Wagner-McPherson,C.B., Evans,G.A. and Tobin,A.J.
  TITLE     Two human glutamate decarboxylases, 65-kDa GAD and 67-kDa GAD, are
            each encoded by a single gene
  JOURNAL   Proc Natl Acad Sci U S A 89 (6), 2115-2119 (1992)
   PUBMED   1549570
REFERENCE   8  (bases 1 to 1965)
  AUTHORS   Martin,D.L., Martin,S.B., Wu,S.J. and Espina,N.
  TITLE     Regulatory properties of brain glutamate decarboxylase (GAD): the
            apoenzyme of GAD is present principally as the smaller of two
            molecular forms of GAD in brain
  JOURNAL   J Neurosci 11 (9), 2725-2731 (1991)
   PUBMED   1880546
REFERENCE   9  (bases 1 to 1965)
  AUTHORS   Erlander,M.G., Tillakaratne,N.J., Feldblum,S., Patel,N. and
            Tobin,A.J.
  TITLE     Two genes encode distinct glutamate decarboxylases
  JOURNAL   Neuron 7 (1), 91-100 (1991)
   PUBMED   2069816
REFERENCE   10 (bases 1 to 1965)
  AUTHORS   Chang,Y.C. and Gottlieb,D.I.
  TITLE     Characterization of the proteins purified with monoclonal
            antibodies to glutamic acid decarboxylase
  JOURNAL   J Neurosci 8 (6), 2123-2130 (1988)
   PUBMED   3385490
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000017.1.
            
            On Nov 26, 2020 this sequence version replaced NM_012563.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M72422.1, SRR26643298.70095.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760393, SAMEA5760433
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-149               JAXUCZ010000017.1  89671718-89671866
            150-209             JAXUCZ010000017.1  89672553-89672612
            210-359             JAXUCZ010000017.1  89672804-89672953
            360-593             JAXUCZ010000017.1  89673928-89674161
            594-684             JAXUCZ010000017.1  89678485-89678575
            685-797             JAXUCZ010000017.1  89679048-89679160
            798-913             JAXUCZ010000017.1  89685036-89685151
            914-993             JAXUCZ010000017.1  89697298-89697377
            994-1048            JAXUCZ010000017.1  89713009-89713063
            1049-1165           JAXUCZ010000017.1  89714756-89714872
            1166-1230           JAXUCZ010000017.1  89716208-89716272
            1231-1309           JAXUCZ010000017.1  89719879-89719957
            1310-1459           JAXUCZ010000017.1  89725211-89725360
            1460-1567           JAXUCZ010000017.1  89728775-89728882
            1568-1657           JAXUCZ010000017.1  89729194-89729283
            1658-1965           JAXUCZ010000017.1  89733939-89734246
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="17"
                     /map="17q12.3"
     gene            1..1965
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /note="glutamate decarboxylase 2"
                     /db_xref="GeneID:24380"
                     /db_xref="RGD:2653"
     exon            1..149
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     CDS             74..1831
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /EC_number="4.1.1.15"
                     /note="glutamic acid decarboxylase 65; GAD-65; 65 kDa
                     glutamic acid decarboxylase; glutamic acid decarboxylase
                     2; Glutamate decarboxylase 2 (islet)"
                     /codon_start=1
                     /product="glutamate decarboxylase 2"
                     /protein_id="NP_036695.1"
                     /db_xref="GeneID:24380"
                     /db_xref="RGD:2653"
                     /translation="MASPGSGFWSFGSEDGSGDPENPGTARAWCQVAQKFTGGIGNKL
                     CALLYGDSEKPAESGGSVTSRAATRKVACTCDQKPCSCPKGDVNYALLHATDLLPACE
                     GERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEIL
                     THCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEY
                     VTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMLIARYKMFPEVKEKGMAAVPRLIAF
                     TSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEVKQKGFVPFLVSA
                     TAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLNGVERANSVTW
                     NPHKMMGVPLQCSALLVREEGLMQSCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHV
                     DVFKLWLMWRAKGTTGFEAHIDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWF
                     VPPSLRVLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAA
                     THQDIDFLIEEIERLGQDL"
     exon            150..209
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            210..359
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            360..593
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            594..684
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            685..797
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            798..913
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            914..993
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1048
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            1049..1165
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1230
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1309
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1459
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1567
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1657
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
     exon            1658..1965
                     /gene="Gad2"
                     /gene_synonym="gad65"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcgtgcaggg tcgagccgaa ggcagcttgc ccgcagccac tcggaggcga ccagcgccag
       61 actagcagaa cccatggcat ctccgggctc tggcttttgg tccttcggat ctgaagatgg
      121 ctctggggat cctgagaacc cgggaacagc gagagcctgg tgccaggtgg cccaaaagtt
      181 cacgggcggc atcggaaaca agctatgcgc tctgctctac ggagactctg agaagccagc
      241 agagagcggc gggagcgtga cctcgcgggc cgccactcgg aaggtcgcct gcacctgtga
      301 ccaaaaaccc tgcagctgcc ccaaaggaga tgtcaattat gcacttctcc acgcaacaga
      361 cctgctgcca gcctgtgaag gagaaaggcc cactctcgca tttctgcaag atgtaatgaa
      421 cattttgctt cagtacgtgg tgaaaagttt tgatagatca actaaagtga ttgatttcca
      481 ttaccccaat gagcttcttc aagagtataa ttgggaattg gcagaccaac cgcaaaatct
      541 ggaggaaatt ttgacgcact gccaaacaac tctaaaatat gcgattaaaa cagggcatcc
      601 ccgatatttt aatcagctgt ctaccggatt ggatatggtt ggattagcag cagattggtt
      661 gacatcaaca gcaaacacga acatgtttac ctatgagatc gcccctgtat ttgtactact
      721 ggaatatgtg acactaaaga aaatgaggga aatcattggc tggccaggag gctctggcga
      781 tggaatcttt tctcctggtg gtgccatctc caacatgtac gccatgctca ttgcccgcta
      841 taagatgttt ccagaagtca aggaaaaggg gatggcggcg gtgcccaggc tcatcgcatt
      901 cacgtcagag catagtcact tttctctcaa gaagggagct gcagccttgg ggatcggaac
      961 agacagcgtg attctgatta aatgtgatga gagagggaaa atgatcccat ctgaccttga
     1021 aagaagaatc cttgaagtca aacagaaagg atttgttcct ttcctggtga gtgccacagc
     1081 tggaaccact gtgtacgggg cttttgatcc tctcttggct gtagctgaca tctgcaaaaa
     1141 atataagatc tggatgcatg tggatgctgc ttggggtgga gggttactga tgtctcggaa
     1201 acacaagtgg aagctgaacg gtgtggagag ggccaactct gtgacatgga atccccacaa
     1261 gatgatgggt gtccccttgc aatgttcggc tctcctggtc agagaggagg gactgatgca
     1321 gagctgcaac cagatgcatg cttcctacct ctttcagcaa gataagcact atgacctgtc
     1381 ctatgacacg ggagacaagg ccttgcagtg tggacgccac gtcgatgtct ttaaattatg
     1441 gctcatgtgg agagcaaagg ggactactgg atttgaagct cacattgata agtgtttgga
     1501 gctggcagag tatttataca atatcattaa aaaccgagaa ggatatgaaa tggtgttcga
     1561 tgggaagcct cagcacacaa atgtctgctt ctggtttgta cctcctagtt tgcgagttct
     1621 ggaagacaat gaagagagaa tgagccgcct ctcaaaggtg gcgccagtga ttaaagccag
     1681 aatgatggag tatgggacca caatggtcag ctaccaaccc ttaggagata aggtcaactt
     1741 cttccgcatg gtcatctcaa accctgcagc aactcaccaa gacattgact tcctcattga
     1801 agaaatcgaa cgcctgggac aagatttgta atcactttgc tcaccaaact ttcagttctc
     1861 taggtagaca gctaagttgt cacaaactgt gtaaatgtat ttgtagtttg ttccagagta
     1921 attctatttc tatatcgtgg tgtcacagta gagtccagtt taaaa
//
