LOCUS       NM_145717                536 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus FXYD domain-containing ion transport regulator 2
            (Fxyd2), transcript variant a, mRNA.
ACCESSION   NM_145717 XM_006242911
VERSION     NM_145717.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Sweadner,K.J., Pascoa,J.L., Salazar,C.A. and Arystarkhova,E.
  TITLE     Post-transcriptional control of Na,K-ATPase activity and cell
            growth by a splice variant of FXYD2 protein with modified mRNA
  JOURNAL   J Biol Chem 286 (20), 18290-18300 (2011)
   PUBMED   21460224
  REMARK    GeneRIF: Post-transcriptional control of Na,K-ATPase activity and
            cell growth by a splice variant of FXYD2 protein with modified
            mRNA.
REFERENCE   2  (bases 1 to 536)
  AUTHORS   Gonzales,P.A., Pisitkun,T., Hoffert,J.D., Tchapyjnikov,D.,
            Star,R.A., Kleta,R., Wang,N.S. and Knepper,M.A.
  TITLE     Large-scale proteomics and phosphoproteomics of urinary exosomes
  JOURNAL   J Am Soc Nephrol 20 (2), 363-379 (2009)
   PUBMED   19056867
REFERENCE   3  (bases 1 to 536)
  AUTHORS   Pihakaski-Maunsbach,K., Vorum,H., Honore,B., Tokonabe,S.,
            Frokiaer,J., Garty,H., Karlish,S.J. and Maunsbach,A.B.
  TITLE     Locations, abundances, and possible functions of FXYD ion transport
            regulators in rat renal medulla
  JOURNAL   Am J Physiol Renal Physiol 291 (5), F1033-F1044 (2006)
   PUBMED   16757733
  REMARK    GeneRIF: the unchanged levels of FXYD2 and FXYD4 proteins suggest
            that they are not primary determinants for urine electrolyte
            composition during NaCl depletion.
REFERENCE   4  (bases 1 to 536)
  AUTHORS   Lindzen,M., Gottschalk,K.E., Fuzesi,M., Garty,H. and Karlish,S.J.
  TITLE     Structural interactions between FXYD proteins and Na+,K+-ATPase:
            alpha/beta/FXYD subunit stoichiometry and cross-linking
  JOURNAL   J Biol Chem 281 (9), 5947-5955 (2006)
   PUBMED   16373350
  REMARK    GeneRIF: Data describe the interactions of rat FXYD1,-2, and -4
            proteins with rat alpha1 subunits of Na(+),K(+)-ATPase.
REFERENCE   5  (bases 1 to 536)
  AUTHORS   Jones,D.H., Li,T.Y., Arystarkhova,E., Barr,K.J., Wetzel,R.K.,
            Peng,J., Markham,K., Sweadner,K.J., Fong,G.H. and Kidder,G.M.
  TITLE     Na,K-ATPase from mice lacking the gamma subunit (FXYD2) exhibits
            altered Na+ affinity and decreased thermal stability
  JOURNAL   J Biol Chem 280 (19), 19003-19011 (2005)
   PUBMED   15755730
REFERENCE   6  (bases 1 to 536)
  AUTHORS   Arystarkhova,E., Donnet,C., Asinovski,N.K. and Sweadner,K.J.
  TITLE     Differential regulation of renal Na,K-ATPase by splice variants of
            the gamma subunit
  JOURNAL   J Biol Chem 277 (12), 10162-10172 (2002)
   PUBMED   11756431
  REMARK    GeneRIF: Differential regulation of renal Na,K-ATPase by splice
            variants of the gamma subunit
REFERENCE   7  (bases 1 to 536)
  AUTHORS   Arystarkhova,E., Wetzel,R.K. and Sweadner,K.J.
  TITLE     Distribution and oligomeric association of splice forms of
            Na(+)-K(+)-ATPase regulatory gamma-subunit in rat kidney
  JOURNAL   Am J Physiol Renal Physiol 282 (3), F393-F407 (2002)
   PUBMED   11832419
REFERENCE   8  (bases 1 to 536)
  AUTHORS   Meij,I.C., Koenderink,J.B., van Bokhoven,H., Assink,K.F.,
            Groenestege,W.T., de Pont,J.J., Bindels,R.J., Monnens,L.A., van den
            Heuvel,L.P. and Knoers,N.V.
  TITLE     Dominant isolated renal magnesium loss is caused by misrouting of
            the Na(+),K(+)-ATPase gamma-subunit
  JOURNAL   Nat Genet 26 (3), 265-266 (2000)
   PUBMED   11062458
REFERENCE   9  (bases 1 to 536)
  AUTHORS   Therien,A.G., Karlish,S.J. and Blostein,R.
  TITLE     Expression and functional role of the gamma subunit of the Na,
            K-ATPase in mammalian cells
  JOURNAL   J Biol Chem 274 (18), 12252-12256 (1999)
   PUBMED   10212192
REFERENCE   10 (bases 1 to 536)
  AUTHORS   Mercer,R.W., Biemesderfer,D., Bliss,D.P. Jr., Collins,J.H. and
            Forbush,B. 3rd.
  TITLE     Molecular cloning and immunological characterization of the gamma
            polypeptide, a small protein associated with the Na,K-ATPase
  JOURNAL   J Cell Biol 121 (3), 579-586 (1993)
   PUBMED   8387529
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000008.1.
            
            On or before Sep 22, 2022 this sequence version replaced
            XM_006242911.4, NM_145717.1.
            
            Summary: This gene encodes a member of a family of small membrane
            proteins that share a 35-amino acid signature sequence domain,
            beginning with the sequence PFXYD and containing 7 invariant and 6
            highly conserved amino acids. This gene, also known as the gamma
            subunit of the Na,K-ATPase, regulates the properties of that
            enzyme. Related gene family members have been shown to induce
            channel activity in experimental expression systems. Transmembrane
            topology has been established for two family members, with the
            N-terminus extracellular and the C-terminus on the cytoplasmic side
            of the membrane. The Type III integral membrane protein encoded by
            this gene is the gamma subunit of the Na,K-ATPase present on the
            plasma membrane. Although the Na,K-ATPase does not depend on the
            gamma subunit to be functional, it is thought that the gamma
            subunit modulates the enzyme's activity by inducing ion channel
            activity. Two transcript variants have been described for this gene
            that encode distinct isoforms. [provided by RefSeq, Jan 2010].
            
            Transcript Variant: This variant (a) encodes the longer isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BQ201573.1, CB719709.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760386, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-83                JAXUCZ010000008.1  54612466-54612548
            84-122              JAXUCZ010000008.1  54614726-54614764
            123-197             JAXUCZ010000008.1  54615051-54615125
            198-234             JAXUCZ010000008.1  54616052-54616088
            235-265             JAXUCZ010000008.1  54616227-54616257
            266-536             JAXUCZ010000008.1  54616518-54616788
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="8"
                     /map="8q22"
     gene            1..536
                     /gene="Fxyd2"
                     /gene_synonym="ATP1C; Atp1g1; GNAKATP"
                     /note="FXYD domain-containing ion transport regulator 2"
                     /db_xref="GeneID:29639"
                     /db_xref="RGD:2173"
     exon            1..83
                     /gene="Fxyd2"
                     /gene_synonym="ATP1C; Atp1g1; GNAKATP"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..259
                     /gene="Fxyd2"
                     /gene_synonym="ATP1C; Atp1g1; GNAKATP"
                     /note="isoform a is encoded by transcript variant a;
                     sodium/potassium-transporting ATPase gamma chain;
                     sodium/potassium-transporting ATPase subunit gamma; sodium
                     pump gamma chain; Na(+)/K(+) ATPase subunit gamma; ATPase,
                     Na+/K+ transporting, gamma 1 polypeptide; gamma subunit of
                     sodium potassium ATPase; FXTD domain-containing ion
                     transport regulator 2"
                     /codon_start=1
                     /product="sodium/potassium-transporting ATPase subunit
                     gamma isoform a"
                     /protein_id="NP_663769.1"
                     /db_xref="GeneID:29639"
                     /db_xref="RGD:2173"
                     /translation="MTELSANHGGSAKGTENPFEYDYETVRKGGLIFAGLAFVVGLLI
                     LLSKRFRCGGSKKHRQVNEDEL"
     exon            84..122
                     /gene="Fxyd2"
                     /gene_synonym="ATP1C; Atp1g1; GNAKATP"
                     /inference="alignment:Splign:2.1.0"
     exon            123..197
                     /gene="Fxyd2"
                     /gene_synonym="ATP1C; Atp1g1; GNAKATP"
                     /inference="alignment:Splign:2.1.0"
     exon            198..234
                     /gene="Fxyd2"
                     /gene_synonym="ATP1C; Atp1g1; GNAKATP"
                     /inference="alignment:Splign:2.1.0"
     exon            235..265
                     /gene="Fxyd2"
                     /gene_synonym="ATP1C; Atp1g1; GNAKATP"
                     /inference="alignment:Splign:2.1.0"
     exon            266..536
                     /gene="Fxyd2"
                     /gene_synonym="ATP1C; Atp1g1; GNAKATP"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 actatccccg tagtggaggc cacaggaaga tcgtggagaa gccctggtgg ctggggaaat
       61 gacagagctg tcagctaacc atggtggcag tgccaagggg acggagaatc ccttcgagta
      121 tgactatgaa accgtccgca aaggaggcct gatcttcgcg ggccttgcct tcgtcgtggg
      181 actcctcatt ctcctcagca aaagattccg ctgtgggggc agtaagaagc ataggcaggt
      241 caatgaagat gagctgtgac ggaagagccg gcagtgccac cggtgcatgg gcaccagtga
      301 tgcccacctt ccccagccag gcaccacgga ccccaccatg gtcagcactg gctgaaggaa
      361 gtgccaggct tactgggacc ccctcatggt catccctcat gcctctagat cgttaacttc
      421 ctctctcccc tccccaacct ctcttacacc caactcccta ttccaagttg gggtacatgt
      481 gcaatctcat tgccctggtt ttcaatgaat cgctctacaa gaatctgcca acaaaa
//
