LOCUS       NM_001245978            9810 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus FRAS1 related extracellular matrix 2 (Frem2),
            mRNA.
ACCESSION   NM_001245978 XM_001057556 XM_227142
VERSION     NM_001245978.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Zhang X, Wang D, Dongye M, Zhu Y, Chen C, Wang R, Long E, Liu Z, Wu
            X, Lin D, Chen J, Lin Z, Wang J, Li W, Li Y, Li D and Lin H.
  TITLE     Loss-of-function mutations in FREM2 disrupt eye morphogenesis
  JOURNAL   Exp Eye Res 181, 302-312 (2019)
   PUBMED   30802441
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   Yu Q, Lin B, Xie S, Gao S, Li W, Liu Y, Wang H, Huang D and Xie Z.
  TITLE     A homozygous mutation p.Arg2167Trp in FREM2 causes isolated
            cryptophthalmos
  JOURNAL   Hum Mol Genet 27 (13), 2357-2366 (2018)
   PUBMED   29688405
REFERENCE   3  (bases 1 to 9810)
  AUTHORS   Palmer K, Fairfield H, Borgeia S, Curtain M, Hassan MG, Dionne L,
            Yong Karst S, Coombs H, Bronson RT, Reinholdt LG, Bergstrom DE,
            Donahue LR, Cox TC and Murray SA.
  TITLE     Discovery and characterization of spontaneous mouse models of
            craniofacial dysmorphology
  JOURNAL   Dev Biol 415 (2), 216-227 (2016)
   PUBMED   26234751
REFERENCE   4  (bases 1 to 9810)
  AUTHORS   Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y,
            Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin
            JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J,
            Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury
            L, Ganapathiraju MK, Tobita K, Pazour GJ and Lo CW.
  TITLE     Global genetic analysis in mice unveils central role for cilia in
            congenital heart disease
  JOURNAL   Nature 521 (7553), 520-524 (2015)
   PUBMED   25807483
REFERENCE   5  (bases 1 to 9810)
  AUTHORS   Seibert FS, Pagonas N, Arndt R, Heller F, Dragun D, Persson P,
            Schmidt-Ott K, Zidek W and Westhoff TH.
  TITLE     Calprotectin and neutrophil gelatinase-associated lipocalin in the
            differentiation of pre-renal and intrinsic acute kidney injury
  JOURNAL   Acta Physiol (Oxf) 207 (4), 700-708 (2013)
   PUBMED   23336369
REFERENCE   6  (bases 1 to 9810)
  AUTHORS   Kiyozumi D, Nakano I, Takahashi KL, Hojo H, Aoyama H and Sekiguchi
            K.
  TITLE     Fused pulmonary lobes is a rat model of human Fraser syndrome
  JOURNAL   Biochem Biophys Res Commun 411 (2), 440-444 (2011)
   PUBMED   21756877
  REMARK    GeneRIF: These findings indicate that the fpl mutant is a rat model
            of human Fraser syndrome.
REFERENCE   7  (bases 1 to 9810)
  AUTHORS   Petrou P, Pavlakis E, Dalezios Y and Chalepakis G.
  TITLE     Basement membrane localization of Frem3 is independent of the
            Fras1/Frem1/Frem2 protein complex within the sublamina densa
  JOURNAL   Matrix Biol 26 (8), 652-658 (2007)
   PUBMED   17596926
REFERENCE   8  (bases 1 to 9810)
  AUTHORS   Chiotaki R, Petrou P, Giakoumaki E, Pavlakis E, Sitaru C and
            Chalepakis G.
  TITLE     Spatiotemporal distribution of Fras1/Frem proteins during mouse
            embryonic development
  JOURNAL   Gene Expr Patterns 7 (4), 381-388 (2007)
   PUBMED   17251066
REFERENCE   9  (bases 1 to 9810)
  AUTHORS   Kiyozumi D, Sugimoto N and Sekiguchi K.
  TITLE     Breakdown of the reciprocal stabilization of QBRICK/Frem1, Fras1,
            and Frem2 at the basement membrane provokes Fraser syndrome-like
            defects
  JOURNAL   Proc Natl Acad Sci U S A 103 (32), 11981-11986 (2006)
   PUBMED   16880404
REFERENCE   10 (bases 1 to 9810)
  AUTHORS   Jadeja S, Smyth I, Pitera JE, Taylor MS, van Haelst M, Bentley E,
            McGregor L, Hopkins J, Chalepakis G, Philip N, Perez Aytes A, Watt
            FM, Darling SM, Jackson I, Woolf AS and Scambler PJ.
  TITLE     Identification of a new gene mutated in Fraser syndrome and mouse
            myelencephalic blebs
  JOURNAL   Nat Genet 37 (5), 520-525 (2005)
   PUBMED   15838507
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB612236.1.
            
            On or before Oct 10, 2011 this sequence version replaced
            XM_227142.5, XM_001057556.2.
            
            Sequence Note: A downstream translational start codon is selected
            for this RefSeq based on its better conservation in mammalian
            species, on a strong Kozak signal, and on the presence of a
            predicted signal peptide in the protein N-terminus. Studies in
            orthologs support the secretion of this protein. An upstream
            in-frame start codon is also present but has a weaker Kozak signal
            and is poorly conserved. The use of the upstream start codon would
            result in a protein that lacks a predicted signal peptide and is 66
            aa longer at the N-terminus. Leaky scanning by ribosomes may allow
            translation initiation at the downstream start codon.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB612236.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5756307,
                                           SAMEA5760383 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="2"
                     /map="2q26"
     gene            1..9810
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /note="FRAS1 related extracellular matrix 2"
                     /db_xref="GeneID:310418"
                     /db_xref="RGD:1566012"
     exon            1..5382
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     CDS             249..9725
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /note="FRAS1-related extracellular matrix protein 2; Fras1
                     related extracellular matrix protein 2"
                     /codon_start=1
                     /product="FRAS1-related extracellular matrix protein 2
                     precursor"
                     /protein_id="NP_001232907.1"
                     /db_xref="GeneID:310418"
                     /db_xref="RGD:1566012"
                     /translation="MATRGRWTAKYSNFQSILTQSPRLLLLLLSLVSRVSTQDAAPGA
                     AFLSLGLSGTSGVPTEEAIVVANRGLRVPFGREVWLDPLRDLVLQVQPGDRCTVAVLD
                     NDALAQRPGRLSPKRFACDYGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAIVLPLA
                     LEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAYQPETEECRVGILSGLSAL
                     PRYGELLHYPQVPGGTGERGSSETPLMDCKAFQELGVRYRHTALSHSPNRDWLPMVVE
                     IHSRGAPVGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDML
                     AAEDAESNPDLLIFNLTSAFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSED
                     SDQERLFELELEVVDPEGAASDPFAFMVVVKPMNTLAPVVTRNTGLILYEGQYRPLTG
                     PVGSGPQNLVISDEDDLEAVRLEVVAGLRHGHLVILGSPSSDPAPKTFTVAELAAGQV
                     VYQHDDRDGSLCDNLVLRMSDGGGRHQVQFLFPITLVPVDDQPPVLNANTGLTVAEGD
                     TVPIPPSTLSATDMDSDDSQLLFVLEPPFSSSGRLLLRQTHAPQEEQGLWHKQGSFYE
                     RMVTEWRQQDITEGKLFYRHSGPHSPGPVMDQFLFRVQDNQDPPNQSGIQRFVIRIHP
                     VDRLPPELGSGCPLRMVVQESQLTPLRKRWLHYTDLDTDDRELHYTVTQPPTDTDENH
                     SPAPLGTLVFTDNPSVVVTHFTQAQVNHHKIAYRPPGQELGVAARVAQFQFQVEDRAG
                     NVAPGTFTLYLQPVDNQPPEIVNTGFTVEEKGHHVLRETELHVSDVDTDVAHISFTLT
                     QAPKHGHLQISGRPLHVGGQFRLEDIKQGRISYLNSGDESLTDSCSLEVSDRHHVVPI
                     TLRVNVRPADREGAMSVLPAGTLESYLDVLENGATEVTANIIKGTYRDTDDLMLTFLL
                     EGPPLYGEILVHGAPAEQFTQRDILEGSVVYAHTSGEIGLLPKADSFNLSLSAMSQEW
                     RIGSSVIQGVTVWVTILPVDSQAPEISVGEQFVVLEGDKSLISLTHLSAEDVDSLMDD
                     LLCTIVIQPTSGYVENISPAPGSEKSRAGIAISAFTLKDLRQGHINYVQSVHRGVEPV
                     EDRFIFRCSDGINFSERQIFPIVIIPTNDEQPEMFMREFMVMEGMSLVVDTPILNAAD
                     ADIPRDDLTFTITRFPTHGHIMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQED
                     SFVIKLTDGKHSVEKMALIMVIPVDDETPRMAINNGLEIEIGETKVINNKVLAATDLD
                     SDDKSLVYIIRYGPGHGLLQRRKPLGVFENITLGMNFTQDEVDRNLIQYVHFGQEGIR
                     DLIKFDVTDGTNALIDRYFYVSIGSVDIVFPDVVSKGVSLKEGGKVTLTTDLLSTSDL
                     NSPDENLVFTITRAPMRGHLECTDQRGLSITSFTQLQLAGNKIYYIHTAEDEVKMDSF
                     EFQVTDGRNPVFRTFRISISDVDNKKPVVTIHNLVVSESESKLITPFELTVEDRDTPD
                     RLLKFIVTQVPVHGHLLFNNTRSVMVFTKQDLNENLISYKHDGTESTEDSFSFTVTDG
                     THSDFYVFPDTVFETRRPQVMKIRVLPVDNRVPQIVVNKGASTLRTLPTGHLGFMITS
                     KILKVQDRDSLHFSLRFIVTEAPQHGYLLNLGQGNHSVTQFTQADIDDMKICYVLRER
                     ANATSDVFHFIVEDDGGNRLTNQHFRLNWAWISFEKEYYLINEDSKFLDIVLKRRGYL
                     GETSFISISTRDGTAEKDKDFKGKAQKQVQFNPGQTRASWRVRILSDGEHEHSETFQV
                     VLSEPVLAVLEFPEVTTVEIIDPGDESTVFIPQSEYSVEEDVSELFIPIRRSGDISQE
                     LMVICYTQQGTATSTVRTSVLSYSDYISRPEDHSSVIRFDKDEREKMCRVVVIDDSLY
                     EEEETFQVLLSMPMGGRIGNKFPGANVTILTDKDDEPAFYFGDTQHSVDESAGYVEVQ
                     VWRTGTDLTKPSSVTVRSRKTEPPSADAGTDYVGISRNLDFAPGVSMQTVRVVILDDL
                     GRPVLEGIEKFELVLRMPMNAALGEPSKATVSINDSASDLPKMQFKERVYTCSENDGH
                     VVAMIYRSGDLQHRSSVRCYTRQGSAQVMMDFEERPNTDISIVTFLPGESEKPCVLEL
                     MDDDLYEDVEELRLVLGTPQGSSAFGAAVGEQNETLIKIQDDADKAVIKFGETKFSVT
                     EPSRPGESVVVKIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQH
                     TVEIEVIFDGVREMREAFTVHLKPDENMVAETQTTKAIVYIEEINSMADVTFPSVPHI
                     VSLMVYDDTFKGREDAGPVSGYPIICITACNPKYPDYDKTGSICASENINDTLTRYRW
                     LTSAPTGPDGVASPMREVDFDTFFTSSKMITLDSIYFQPGSRVQCAARAVNTNGNEGL
                     ELMSPIVTIAREEGLCQPRVPGVVGAEPFSAKLRYTGPEDPDFANLIKLTVTMPHIDG
                     MLPVISTRELSNFELTLSPDGTRVGNHKCSNLLDYSEVRTHHGFLTNATKNPEVIGET
                     YPYQYSAPVRGSSTLRFYRNLNLEACLWEFVSYYDMSELLADCGGTIGTDGQVLNLVQ
                     SYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWNHGIGSPPEAELQ
                     GSLYPTSMRIGEEGRLAVNFKTEAQFHGLFVLSHPASFTSSLIVSADHPGLTFSLRLI
                     RSEPTYNQPVQQWSFISDFAVRDYSGTYTVKLVPCTTPSNQEYRLPVTCNPREPVTFD
                     LDIRFQQVSDPVAAEFSLNTHMYLLSKKSLWLSDGSMGFGQESDVAFAEGDVIYGRVM
                     VDPVQNLGDSFYCSIEKVFLCTGDDGYVPKYSPANAEYGCLADSPSLLHRFKIVDKAQ
                     PETQATSFGDVLFNAKLAVDDPEAALLVNQPGSDGFKVDSTPLFQVALGREWYIHTIY
                     TVRSKGTSHRGIGKRSLEYQYHSVVQHGPPQATTKSWKKRAVRSTPSLAGEIGAENNR
                     GTNIQHISLDRRGKRQVPHGRIPPDGILPWELNSPSSEMSLVTVLGGISVGLLAVCLA
                     VAVAVMCRNRSIKGKDAPKGSGSTEPMMSPQSHYNDSSEV"
     exon            5383..5472
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            5473..5619
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            5620..5850
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            5851..5976
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            5977..6228
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            6229..6378
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            6379..6588
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            6589..6786
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            6787..6951
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            6952..7134
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            7135..7265
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            7266..7424
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            7425..7728
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            7729..7860
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            7861..8192
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            8193..8385
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            8386..8490
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            8491..8618
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            8619..8753
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            8754..8880
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            8881..9068
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            9069..9215
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
     exon            9216..9810
                     /gene="Frem2"
                     /gene_synonym="RGD1566012"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acacgccctg gacgcgctgg aagtggtcta gtaccctggg ttccagctcc atggcgggac
       61 cgcgcggcgc acttggccat cctcctggtc cagccctgcc actcggaggg ccccgcaggc
      121 gccgcgcaga gttcccggcc cttcccatcg ggctctggag gagcctgcaa agactggctt
      181 tgcatactcg ccgagtgaac tgtcggaact ctaacgcctg caacctgcat gctacaagga
      241 cccccgggat ggccacccgc gggcgctgga cagccaagta ctccaacttc cagtctatac
      301 tcacacagtc gcccagactg ctgctgctgc tcctgtcact ggtaagccgc gtctccacac
      361 aggatgcagc ccctggcgct gcatttctat ccctgggtct ctccgggact tcgggggtcc
      421 ctaccgagga agctatagtg gtggcgaacc gcgggctccg tgtgcccttc ggtcgcgaag
      481 tctggctgga tccgttgcgt gacttggtgc tgcaggtgca gccaggagac cgctgcacag
      541 tggccgtgct agacaacgac gcgctggccc agcggcccgg ccgcttgagt cccaagcgtt
      601 ttgcgtgcga ctacggcccg ggtgaggtgc gttactcgca cctgggtgct cgcagccctt
      661 cccgcgaccg cgtccggctg cagctgcgct acgatgctcc aggaggggcc atagtcttgc
      721 cattggcact agaggtggaa gtggttttca cccagctgga ggttgtgact cggaatttac
      781 ctctggtcgt ggaagaactg ctagggacca gtaatgccct ggatgctcgg agcttagagt
      841 tcgcctacca gcctgagacc gaggagtgcc gcgtgggcat cttgtcaggt ttgagcgctt
      901 tgcctcgtta tggagaactc ctgcactacc cacaggtccc gggagggacc ggagagagag
      961 ggtcctccga gacccctttg atggactgca aagcattcca ggagctggga gtgcgctatc
     1021 gtcacacagc cctgagtcac tcacctaaca gggactggct gcctatggtg gtggagatac
     1081 attcaagagg ggctccagtg ggcagcccgg ctttaaagcg tgagcatttc caggtgcttg
     1141 tgaggatccg gggaggtgcg gagaacactg cgcccaagcc cagtttcgtg gccatgatga
     1201 tgatggaggt agaccagttt gtactgactg ctctcactcc agacatgctg gcagcagagg
     1261 atgctgaatc caacccggac cttttgattt tcaacctcac ctctgccttc cagcctgggc
     1321 aaggctactt ggtgagcact gatgatagaa gcctgcctct gtcctccttt acacagcggg
     1381 atttgcgcct gctgaagatt gcctaccagc ctccctctga agactccgat caggagcgcc
     1441 tctttgagct agagctggag gtagtggacc ctgaaggagc agcctcagac cctttcgcct
     1501 tcatggtggt ggtgaaaccc atgaacacac tggctccagt ggtcacacgg aacactggcc
     1561 tcattctcta tgagggtcag tatcggccac tcacgggtcc tgtaggcagt ggaccccaga
     1621 acttggtcat cagtgatgaa gatgacctag aagctgtgag actggaggtg gtggccgggc
     1681 tccggcatgg tcaccttgtt attctgggtt ctcccagtag tgaccctgct cccaagacct
     1741 tcactgtggc tgagctggcc gctggccagg tggtctacca gcatgatgac agagacggtt
     1801 cactgtgtga caacttggtg ctgcgcatgt cagatggagg aggcaggcac caggtccagt
     1861 tcctgttccc tatcactctg gtgcctgtgg atgaccagcc acctgtcctg aacgccaaca
     1921 ctgggctgac agtggcagag ggtgacaccg tgccaatccc gccctcgact ctgagtgcaa
     1981 ctgatatgga ctcggatgac tctcagctgc tttttgtact ggagccgccc ttctcaagct
     2041 caggacgcct gcttctccgc caaacgcatg ctccccagga agagcagggt ctgtggcaca
     2101 aacaagggtc cttttatgag cgaatggtga cagagtggcg gcaacaggat ataacggagg
     2161 ggaaactctt ctacaggcac tcggggcccc acagccctgg gccagtgatg gaccagttcc
     2221 tgtttagagt ccaagacaat caagaccccc ctaatcagtc tgggatccag aggtttgtaa
     2281 tacgcatcca tcccgtggac cgtctccctc cagaactggg cagtggttgt ccccttcgga
     2341 tggtggtaca ggaatcccag ctcacaccac tgaggaaaag gtggctgcac tacactgacc
     2401 tagacacaga cgaccgagag ctccactaca cagtgaccca gccccctacg gacacagatg
     2461 agaaccactc accggctccg ctggggactc tggtcttcac tgataatccc tcagttgtgg
     2521 tgacccattt tacccaagcc caggtcaacc atcataaaat tgcttacagg cccccagggc
     2581 aggagctagg agtagcagcc cgagtggctc agttccagtt ccaagtagag gatcgagctg
     2641 ggaatgtggc tccaggcacc ttcaccctct acctgcagcc tgtggataac cagccccccg
     2701 agattgtcaa cacgggcttc actgttgagg agaagggcca ccacgtcctt cgtgagactg
     2761 agctgcatgt gagtgacgtt gacacggatg tggcccacat ctcgttcact ctcacacagg
     2821 cccccaaaca tggccacttg caaatatctg gacggccact gcatgtagga ggacagttcc
     2881 gtttagagga cataaagcag ggcagaattt cctatctgaa tagtggggac gaatctttga
     2941 ctgacagctg ctccctggag gtcagcgaca gacaccacgt ggtgccaatc actctcagag
     3001 tgaacgttcg gccagcggac cgtgaaggtg ctatgtcagt ccttcctgct ggtactctgg
     3061 agtcctatct agatgttttg gagaatgggg ctaccgaagt cactgccaac atcattaagg
     3121 ggacctatcg ggataccgat gatttgatgt tgactttcct gttggaaggt ccgcccttat
     3181 atggggagat cctggtccac ggagctccag ccgagcagtt cacccagagg gacatcctgg
     3241 agggctctgt tgtctacgct cataccagtg gtgagattgg cctgttgccc aaagcagact
     3301 cttttaacct gagtctgtca gctatgtctc aagaatggag aattggtagc agtgttatcc
     3361 aaggcgttac tgtgtgggtg accatcctcc ccgtggacag ccaagcccct gaaatttctg
     3421 ttggtgagca gtttgtggtg ctggaagggg acaagagttt gataagcctg acccacctaa
     3481 gtgctgagga cgtggactcc ctcatggatg acctcttgtg cacaatagtc attcagccaa
     3541 cctcgggcta tgtagagaac atttctccag caccaggctc agaaaaatca agggctggga
     3601 ttgccataag tgccttcacg ctgaaggatc tcaggcaagg acacattaac tatgtccaga
     3661 gtgtccacag aggggtggag cctgtggaag accgctttat attccgttgt tctgacggca
     3721 ttaacttttc tgagagacag attttcccca ttgtaatcat tcctaccaac gatgagcagc
     3781 cagaaatgtt catgagggaa ttcatggtga tggaaggcat gagtctggtg gtcgatacac
     3841 ccatcctcaa cgctgccgat gccgacattc cccgggatga tttaacgttc accattaccc
     3901 ggtttccgac tcatggtcat atcatgaacc agctgatcaa tggtacagtg ttggttgaaa
     3961 gcttcacctt ggaccagatt atagagagtt ccagcattat ttatgagcat gatgactctg
     4021 agacacaaga ggacagtttt gtgattaagt tgacagatgg caagcactct gtggagaaga
     4081 tggccctcat catggtcatc cctgttgatg acgagacgcc cagaatggcc atcaataacg
     4141 ggctagaaat agaaattggg gaaaccaaag ttatcaataa caaagttcta gcggcaactg
     4201 atttagactc tgatgacaaa tcactggttt atattattcg gtatggaccg gggcatggct
     4261 tgttacagag acggaagcct ctaggtgtct ttgaaaatat cacgctaggc atgaacttta
     4321 cccaggatga agtggacagg aacttaattc aatatgtcca ctttgggcaa gagggcattc
     4381 gagacctaat taaattcgat gtgacagatg ggacgaatgc tctcatagac cgctacttct
     4441 acgtgtctat tggaagtgtt gacattgtct tccctgatgt ggtaagtaag ggcgtgtcct
     4501 tgaaagaagg tggcaaggtc actctgacaa ccgacctgct aagcacaagc gacctgaaca
     4561 gtcctgatga gaacttagtt ttcaccatta ccagggctcc catgagaggt cacctggagt
     4621 gcacggacca aagaggactg tccatcactt ctttcactca gctccagctg gctggcaaca
     4681 aaatctacta catacacaca gccgaggatg aggtcaaaat ggacagtttc gagtttcaag
     4741 tcaccgacgg ccggaaccct gtcttccgaa ctttccggat ctcaatcagc gacgtagaca
     4801 acaaaaagcc agtggtcacc atccacaact tggtggtcag tgaaagtgaa agtaagctta
     4861 tcaccccctt tgaactcacc gtggaagaca gagatacccc cgacaggctc ctaaaattca
     4921 tcgtcaccca ggtccctgtt catggtcacc tcctcttcaa caacaccaga tctgtcatgg
     4981 ttttcaccaa gcaagacttg aatgaaaact taatcagcta caaacacgat ggcacagaat
     5041 ccacggaaga cagcttctcc ttcacggtga ctgacggcac ccactcggac ttctatgttt
     5101 ttcctgatac agtgtttgag actaggagac ctcaagtgat gaaaatccgg gtgttacctg
     5161 ttgacaaccg tgtcccccaa attgtagtga acaaaggagc ttctacactt cggactctgc
     5221 ccaccggcca tttggggttc atgatcacga gcaaaatatt gaaagtgcag gacagagaca
     5281 gcctgcattt ttctctcagg ttcattgtga cagaagcccc ccaacatggc tatcttctca
     5341 acctgggcca aggaaaccac agtgtcacac agtttaccca agctgacatt gacgacatga
     5401 aaatatgcta tgtcttgaga gagagagcaa atgccacgag cgatgtgttc catttcatag
     5461 tggaagatga cggtggaaac aggttaacca accagcattt tcgactgaat tgggcatgga
     5521 tctcttttga aaaggagtat tacctgatca acgaagattc caaatttctg gatattgttc
     5581 ttaaacgcag aggatacttg ggagaaactt cgtttataag tatcagcaca agggatggga
     5641 ctgcagaaaa agacaaagac ttcaagggta aagctcagaa acaagtgcag ttcaatccgg
     5701 gccaaaccag ggcctcgtgg agagtgcgga tcctgagcga tggggaacac gagcactccg
     5761 agaccttcca agtggttctc tctgagccgg tgctggcagt cctggagttc ccggaagtga
     5821 ctacggtgga gatcattgac ccaggcgatg aatcaaccgt gttcattccc cagtctgaat
     5881 actcagttga agaagatgtg agcgagctgt tcattcccat caggaggagt ggagacatca
     5941 gccaggagct gatggtgatc tgctatacac agcaagggac agcaaccagc actgtgcgga
     6001 catccgtgct gtcttactct gactacatct ccaggcctga ggaccacagc agcgtgatcc
     6061 gctttgacaa agatgaacgt gagaaaatgt gtcgtgtggt ggtgattgat gactcgttgt
     6121 acgaagagga agagactttc caggtccttt tgagcatgcc catgggcggg aggatcggca
     6181 acaagtttcc gggagccaat gtgaccatcc tcacagacaa ggatgatgag cctgccttct
     6241 actttgggga tacccagcat tcggtggatg aaagtgctgg ctacgtggag gtgcaggtgt
     6301 ggaggacggg gaccgacctc acgaagcctt ctagtgtcac ggtgaggtcc cggaaaacag
     6361 aacctccctc tgcagacgct ggaacagatt atgtgggaat cagtcgcaat ttagacttcg
     6421 cacctggcgt cagcatgcag accgttcgcg ttgtcatcct cgatgacctg ggacgaccag
     6481 tgctggaggg aatcgagaag tttgaactgg tacttcgaat gcctatgaac gctgcccttg
     6541 gcgaacccag caaggccacc gtgtccataa atgactctgc ctcagacttg cccaaaatgc
     6601 aattcaaaga aagagtgtac acttgcagcg aaaatgatgg acacgtggtt gcaatgatct
     6661 acaggagtgg tgacctccag cacaggtctt ctgtgagatg ctacacgagg caggggtctg
     6721 cccaggtgat gatggatttt gaagaacgcc caaataccga catttccatt gtcacattcc
     6781 tccctggtga gtcggagaag ccgtgtgtcc ttgaactgat ggacgatgat ctctatgagg
     6841 acgtggagga gctgcgcctg gtcctcggca caccacaggg cagctctgcc tttggagctg
     6901 cagtgggcga gcagaacgaa accctgataa agatccagga tgatgctgac aaagctgtta
     6961 tcaaatttgg agaaaccaaa tttagtgtca ctgaacccag cagaccagga gagtcggtgg
     7021 ttgtcaaaat tccggtgatc cgccaaggag acacttcgaa ggtttccatc gtgagagtgc
     7081 acaccaagga tggctcggct acctctggag aggattacca ccctgtgtcg gaagaaatcg
     7141 agtttaagga aggggaaacg caacacactg ttgaaattga agtaatcttc gatggggtga
     7201 gagagatgag agaggctttc actgttcacc ttaaaccgga tgaaaatatg gtagcagaga
     7261 cacagacaac caaggccatt gtatatatag aagaaatcaa cagcatggca gatgtcacct
     7321 ttccttctgt ccctcacatt gtgtccttga tggtatatga tgacactttc aaaggcagag
     7381 aggacgctgg acctgtttct ggctatccta tcatctgcat tacggcctgc aaccccaaat
     7441 atccagacta tgacaaaaca ggctctattt gtgccagtga gaacatcaac gacactctga
     7501 cccgatacag atggctgacc agtgctccca ctggcccgga tggtgtagcc agccctatga
     7561 gagaggtgga cttcgacacc ttcttcacat catccaagat gatcacacta gactccatat
     7621 actttcagcc aggctctcgg gtacagtgtg cggcccgagc agtgaacacc aatgggaacg
     7681 aaggcctgga actaatgagc cccatcgtca ccattgccag agaggaaggt ctctgtcagc
     7741 cccgtgtgcc tggggtagtg ggggcagagc cgttctcagc aaagttgcgc tacactggcc
     7801 cggaggaccc agactttgcc aacctcatca agctcacggt cacgatgcca cacatagacg
     7861 gcatgctccc ggtcatctcc acgagagagc tgtccaactt tgagctgacg ctcagcccag
     7921 atggcacaag agttgggaat cacaaatgct ccaacctgct ggactacagt gaagtgagga
     7981 cccaccatgg cttcctgact aatgccacga agaacccgga ggtcattgga gagacctatc
     8041 cttaccagta cagtgcgccg gtcaggggtt ccagcacctt acgcttctac cgaaacctga
     8101 acctggaggc ctgtctgtgg gagtttgtca gctactacga catgtcggag ctcctggctg
     8161 actgtggagg aaccatcggg accgatggcc aggtcctcaa cctcgtgcag tcctacgtga
     8221 cccttcgagt tcctctgtat gtttcctacg tgttccactc ccccgtgggc gtgggaggct
     8281 ggcagcattt cgacttgaag tccgagctcc ggctcacatt cgtgtacgac actgccatct
     8341 tgtggaacca tgggatcggc agccccccgg aagctgaact gcaaggctct ctctatccaa
     8401 ccagcatgcg cattggtgag gagggacgcc tggctgtgaa cttcaagaca gaagctcagt
     8461 tccacggctt gtttgtgctg tcccatcctg cttccttcac cagctcattg attgtgtcag
     8521 ctgaccatcc gggcctcaca ttttccctcc gccttatacg gagtgaacca acctacaatc
     8581 agccagtcca gcagtggagt ttcatctcag actttgcagt tcgtgactac tcgggcacct
     8641 acacagtaaa gctggtgcct tgcaccaccc catccaacca ggagtaccgt ttgccggtca
     8701 cctgcaaccc cagagagcct gtcacctttg accttgacat tcgattccaa caggtcagcg
     8761 accccgtggc agctgagttc agcctgaaca cccacatgta tctgctgtct aagaagagtc
     8821 tctggttgtc tgatgggtcc atgggatttg ggcaggagag cgatgttgcc tttgcagaag
     8881 gggacgtgat ttatggtcgt gtcatggtgg atcctgtcca gaaccttggt gactcctttt
     8941 actgcagcat tgagaaggtg ttcttatgca cgggggatga tggctacgtg cccaagtaca
     9001 gcccggcaaa tgcagaatat ggctgcctgg ctgattctcc ttcactctta catagattca
     9061 aaattgtgga taaggctcag ccagagacac aagccacaag ctttggagat gtcctgttta
     9121 acgccaaact ggcggtggat gaccctgaag cagctctctt agtgaatcag cctggatcag
     9181 atggttttaa agtagactca acgccactct ttcaggttgc cttgggccga gaatggtaca
     9241 tacacacaat ctatacggtg aggtcaaagg gcacctccca tcgaggcatc ggcaagagga
     9301 gcctggagta ccagtatcac tctgtggtgc agcacggacc gccccaggca actaccaaga
     9361 gctggaagaa gagagcagtc aggagcacgc cctcactcgc aggggaaatc ggtgccgaga
     9421 acaatcgagg aacaaatatc caacacatct ccctggaccg ccgtggcaag aggcaggtcc
     9481 cccatggaag aattcctcct gacggcatcc tgccctggga gctcaatagt cccagctctg
     9541 aaatgagcct tgtcactgtc ctgggaggca tctcggtggg tttgcttgct gtctgcttgg
     9601 ctgtggccgt ggcagtcatg tgcaggaacc ggagtatcaa gggcaaggat gctccaaagg
     9661 gctctgggag cactgagccc atgatgtccc cgcagagcca ctacaatgac agctcagagg
     9721 tgtgatgacc ttctcttcga gtgcctcaga ggacggggca ggaaccccga ctgcttgggg
     9781 gcagcggcag agaaccgaga actttctgtg
//
