LOCUS       NM_001411535            9478 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus Fras1 related extracellular matrix 1 (Frem1),
            mRNA.
ACCESSION   NM_001411535 XM_006238349
VERSION     NM_001411535.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Takahashi,T., Friedmacher,F. and Puri,P.
  TITLE     Gene Expression of FRAS1-Related Extracellular Matrix 1 Is
            Decreased in Nitrofen-Induced Congenital Diaphragmatic Hernia
  JOURNAL   Eur J Pediatr Surg 26 (1), 81-85 (2016)
   PUBMED   26382659
  REMARK    GeneRIF: Decreased expression of FREM1 in the nitrofen-induced
            congenital diaphragmatic hernia model may disturb the formation of
            the diaphragmatic mesenchyme, thus contributing to the development
            of diaphragmatic defects.
REFERENCE   2  (bases 1 to 9478)
  AUTHORS   Beck,T.F., Veenma,D., Shchelochkov,O.A., Yu,Z., Kim,B.J.,
            Zaveri,H.P., van Bever,Y., Choi,S., Douben,H., Bertin,T.K.,
            Patel,P.I., Lee,B., Tibboel,D., de Klein,A., Stockton,D.W.,
            Justice,M.J. and Scott,D.A.
  TITLE     Deficiency of FRAS1-related extracellular matrix 1 (FREM1) causes
            congenital diaphragmatic hernia in humans and mice
  JOURNAL   Hum Mol Genet 22 (5), 1026-1038 (2013)
   PUBMED   23221805
  REMARK    Erratum:[Hum Mol Genet. 2020 Apr 15;29(6):1054. PMID: 32016392]
REFERENCE   3  (bases 1 to 9478)
  AUTHORS   Beck,T.F., Shchelochkov,O.A., Yu,Z., Kim,B.J., Hernandez-Garcia,A.,
            Zaveri,H.P., Bishop,C., Overbeek,P.A., Stockton,D.W., Justice,M.J.
            and Scott,D.A.
  TITLE     Novel frem1-related mouse phenotypes and evidence of genetic
            interactions with gata4 and slit3
  JOURNAL   PLoS One 8 (3), e58830 (2013)
   PUBMED   23536828
REFERENCE   4  (bases 1 to 9478)
  AUTHORS   Vissers,L.E., Cox,T.C., Maga,A.M., Short,K.M., Wiradjaja,F.,
            Janssen,I.M., Jehee,F., Bertola,D., Liu,J., Yagnik,G.,
            Sekiguchi,K., Kiyozumi,D., van Bokhoven,H., Marcelis,C.,
            Cunningham,M.L., Anderson,P.J., Boyadjiev,S.A., Passos-Bueno,M.R.,
            Veltman,J.A., Smyth,I., Buckley,M.F. and Roscioli,T.
  TITLE     Heterozygous mutations of FREM1 are associated with an increased
            risk of isolated metopic craniosynostosis in humans and mice
  JOURNAL   PLoS Genet 7 (9), e1002278 (2011)
   PUBMED   21931569
REFERENCE   5  (bases 1 to 9478)
  AUTHORS   Slavotinek,A.M., Baranzini,S.E., Schanze,D., Labelle-Dumais,C.,
            Short,K.M., Chao,R., Yahyavi,M., Bijlsma,E.K., Chu,C., Musone,S.,
            Wheatley,A., Kwok,P.Y., Marles,S., Fryns,J.P., Maga,A.M.,
            Hassan,M.G., Gould,D.B., Madireddy,L., Li,C., Cox,T.C., Smyth,I.,
            Chudley,A.E. and Zenker,M.
  TITLE     Manitoba-oculo-tricho-anal (MOTA) syndrome is caused by mutations
            in FREM1
  JOURNAL   J Med Genet 48 (6), 375-382 (2011)
   PUBMED   21507892
REFERENCE   6  (bases 1 to 9478)
  AUTHORS   Manabe,R., Tsutsui,K., Yamada,T., Kimura,M., Nakano,I., Shimono,C.,
            Sanzen,N., Furutani,Y., Fukuda,T., Oguri,Y., Shimamoto,K.,
            Kiyozumi,D., Sato,Y., Sado,Y., Senoo,H., Yamashina,S., Fukuda,S.,
            Kawai,J., Sugiura,N., Kimata,K., Hayashizaki,Y. and Sekiguchi,K.
  TITLE     Transcriptome-based systematic identification of extracellular
            matrix proteins
  JOURNAL   Proc Natl Acad Sci U S A 105 (35), 12849-12854 (2008)
   PUBMED   18757743
REFERENCE   7  (bases 1 to 9478)
  AUTHORS   Petrou,P., Pavlakis,E., Dalezios,Y. and Chalepakis,G.
  TITLE     Basement membrane localization of Frem3 is independent of the
            Fras1/Frem1/Frem2 protein complex within the sublamina densa
  JOURNAL   Matrix Biol 26 (8), 652-658 (2007)
   PUBMED   17596926
REFERENCE   8  (bases 1 to 9478)
  AUTHORS   Chiotaki,R., Petrou,P., Giakoumaki,E., Pavlakis,E., Sitaru,C. and
            Chalepakis,G.
  TITLE     Spatiotemporal distribution of Fras1/Frem proteins during mouse
            embryonic development
  JOURNAL   Gene Expr Patterns 7 (4), 381-388 (2007)
   PUBMED   17251066
REFERENCE   9  (bases 1 to 9478)
  AUTHORS   Kiyozumi,D., Sugimoto,N. and Sekiguchi,K.
  TITLE     Breakdown of the reciprocal stabilization of QBRICK/Frem1, Fras1,
            and Frem2 at the basement membrane provokes Fraser syndrome-like
            defects
  JOURNAL   Proc Natl Acad Sci U S A 103 (32), 11981-11986 (2006)
   PUBMED   16880404
REFERENCE   10 (bases 1 to 9478)
  AUTHORS   Smyth,I., Du,X., Taylor,M.S., Justice,M.J., Beutler,B. and
            Jackson,I.J.
  TITLE     The extracellular matrix gene Frem1 is essential for the normal
            adhesion of the embryonic epidermis
  JOURNAL   Proc Natl Acad Sci U S A 101 (37), 13560-13565 (2004)
   PUBMED   15345741
COMMENT     INFERRED REFSEQ: This record is predicted by genome sequence
            analysis and is not yet supported by experimental evidence. The
            reference sequence was derived from JAXUCZ010000005.1.
            
            On Aug 26, 2022 this sequence version replaced XM_006238349.4.
            
            ##Evidence-Data-START##
            CDS exon combination :: SRR26360172.870784.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA5760383,
                                    SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-264               JAXUCZ010000005.1  102515141-102515404 c
            265-778             JAXUCZ010000005.1  102486976-102487489 c
            779-873             JAXUCZ010000005.1  102482680-102482774 c
            874-1175            JAXUCZ010000005.1  102480242-102480543 c
            1176-1372           JAXUCZ010000005.1  102478416-102478612 c
            1373-1696           JAXUCZ010000005.1  102470282-102470605 c
            1697-1805           JAXUCZ010000005.1  102466272-102466380 c
            1806-1937           JAXUCZ010000005.1  102463718-102463849 c
            1938-2282           JAXUCZ010000005.1  102461924-102462268 c
            2283-2425           JAXUCZ010000005.1  102460819-102460961 c
            2426-2622           JAXUCZ010000005.1  102456085-102456281 c
            2623-2713           JAXUCZ010000005.1  102455022-102455112 c
            2714-2881           JAXUCZ010000005.1  102453644-102453811 c
            2882-3090           JAXUCZ010000005.1  102450685-102450893 c
            3091-3184           JAXUCZ010000005.1  102448027-102448120 c
            3185-3437           JAXUCZ010000005.1  102444843-102445095 c
            3438-3632           JAXUCZ010000005.1  102436686-102436880 c
            3633-3818           JAXUCZ010000005.1  102435249-102435434 c
            3819-4015           JAXUCZ010000005.1  102433942-102434138 c
            4016-4202           JAXUCZ010000005.1  102430844-102431030 c
            4203-4347           JAXUCZ010000005.1  102428268-102428412 c
            4348-4489           JAXUCZ010000005.1  102424241-102424382 c
            4490-4685           JAXUCZ010000005.1  102420753-102420948 c
            4686-4950           JAXUCZ010000005.1  102415287-102415551 c
            4951-5362           JAXUCZ010000005.1  102406080-102406491 c
            5363-5564           JAXUCZ010000005.1  102401832-102402033 c
            5565-5709           JAXUCZ010000005.1  102400850-102400994 c
            5710-5839           JAXUCZ010000005.1  102392971-102393100 c
            5840-5912           JAXUCZ010000005.1  102390718-102390790 c
            5913-6062           JAXUCZ010000005.1  102385005-102385154 c
            6063-6286           JAXUCZ010000005.1  102383002-102383225 c
            6287-6334           JAXUCZ010000005.1  102382408-102382455 c
            6335-6499           JAXUCZ010000005.1  102381952-102382116 c
            6500-6628           JAXUCZ010000005.1  102381623-102381751 c
            6629-6744           JAXUCZ010000005.1  102380951-102381066 c
            6745-6830           JAXUCZ010000005.1  102372765-102372850 c
            6831-9478           JAXUCZ010000005.1  102367201-102369848 c
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q31"
     gene            1..9478
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /note="Fras1 related extracellular matrix 1"
                     /db_xref="GeneID:298185"
                     /db_xref="RGD:1306981"
     exon            1..264
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            265..778
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     CDS             527..7030
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /note="FRAS1-related extracellular matrix protein 1"
                     /codon_start=1
                     /product="FRAS1-related extracellular matrix protein 1
                     precursor"
                     /protein_id="NP_001398464.1"
                     /db_xref="GeneID:298185"
                     /db_xref="RGD:1306981"
                     /translation="MHSPGCTGPKALLFLLLQLLMLHLDPANATFISINRGLRVMKGS
                     SAFLSGDHLRFAIPKEKDACRVEVVMNEPITQRVGKLIPQVFDCHFLPNEVQYVHNGC
                     PILDEDSVKLRLYRFTETETFMETFLLRVSLVEPDCNIIRLSSNVLEVTEFYGLSQAI
                     DKNLLQFDYDRTASLDCTIRVDPVKTQLPAHGKLVVVNRKSEGPRGDQPHSFFSESQL
                     GAERKCPDGSCTLEPKQVTSLKVSCEDFLLTGFHYQHMQPPSPNIDYIPIQLDLTDRR
                     SKTIYKSESAWLPVFIRVGIPNQAPRAAFMAMFVLEVDQFILTPLTTSVLDCEEDETP
                     KPLLVFNITEAPLQGYVTHLLDHTRPISSFTWKDLSDMQVAYQPPNNSHPERRHYKME
                     LEVYDLFFERSAPITVHISIRTADTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDD
                     IGAVQLVTIGGLQHGRLTVREGKGFLFTVADLQAGVVRYHHDDSDTTKDFVAFRIFDG
                     QHSSHHKFPINILPKDDSPPFLITNVVIELEEGKTILIQGSMLRASDMDSSDDYIFFN
                     ITKFPQAGEIMKKPGPRLIGYPVSGFLQRDLFSGIIYYRHFGGEIFEDSFEFVLWDSH
                     EPPNLSVPQVVMVHITPVDDQLPKEAPGISRHLIVKETEVAYITKKHLHFLDTESHGG
                     ELVYTITRPPCFSSSHRHLDAGKLFMVDSIPKPTKNPTAPGLNSFTQHAVNHMKVAYM
                     PPMQDIGPTPRHVQFTVSVSNQHGGTLHGICFNITVLPVDNQVPEVFTNTLRVAEGGQ
                     CTISTEHILVSDVDTPLDSICLSLQEMPLHGGVELDGFPLNPRGTFSWRDLHTLKVRY
                     QHDGSEVLQDEIFLEVTDGTNSAGFVLYIEVFPVNDEPPVLKADLIPMMHCSEGGEVT
                     ITPEYIFATDADSNDSELLFLITREPRHGVVRKAGLRVDRFSQGDVISGAVAYKHTGG
                     EIGLESCFDTVVLVVSDGEADPLVNGCCYNGPDSSVPLHKSFPTYQLNITVHPVDNQP
                     PSIIIGRTLTVDEGFSAALTTHHLTATDWDTAPDDLEFILVSHPQFGYLENTLPSAGF
                     EKRNTGISIASFQWRDMKASHINYVQSRHLREEPTADEFLVYATDGKQRSLETTFHVI
                     INPTNDEAPDFVVQNITVYEGHMVELDSAIISATDRDVPKDPLLFNITHKPKHGLLLI
                     SATSKDAHNEIHNFSMELLQNGMKLAYAHDDSESSADNFVIQLSDGKHKILKTVSVSI
                     TPVNDETPTLSKKAEVSVTVGDTRVLSSAVLSAIDKDSPREKIHYLFERLPQNGQLQL
                     KIGRDWVPLSAGMQCTQEDVDLNLLRYAHAGKMHSQDGDRFTFYLWDGDNRSPAFDCH
                     IIIEDTGRGDIVIHAKPLVVANGDRGLLTPATLLAEDGADKAEELLYLITSPPQYGQV
                     EYVYSPGVPITSFSQMDIAGQAVCYIHKSRTAVLSDSFRFTISNGLQTQQGVFEITLQ
                     GVDSALPVLTRNRRLRLVEGAMGLLSTDHLQLTDPDTPPENLTFFLVQLPRHGYLYLR
                     GKVLQHNFTQQDVDSRVVAYQHAGGGAQEDCFTFLATDRKNQGFVVDGKVQKEPVLFI
                     IQVDQLDKKAPRITRLHSPSQVGLLKNGCYGIYITSHVLKASDPDTEDDQIIFKILRG
                     PLHGRLENTTTGEFIHEQFSQKDLSRKTILYIINPSLQVNSDILEFQAMDPTGNTAPP
                     QRLELKWSYIEWSQTEYEVCENVGLLTLEVTRRGYSMDSAYVDIEVNQVSAVVGKDFT
                     VTPSKLLQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTQTKAAVKILD
                     SKGGRCHPSNSFNQSKHSTWGKGRWHPLPSGSSSLTTSGSPHLERRPPPSITKGDALR
                     GFDLTDLSQRKMTTQGNGKSVLPSSVCRNGTDTIYNYHGIVSLKLEGDRFSAHKRKAK
                     ISVIRQPQRTVKVAELPLADKVEPTTDSNFLSQGLRPLFPKNCSVELKGLFHLEEHTH
                     RLYQCDGIAWNVWSSQTKGLEDRSCPVGWLLHSDYCHVLVTRQKGTWATATRACREQH
                     QGTLVTVFSRRHMQWLWAISGRKPFWIGLKNQVRTGHWEWIGGEPVAFTNWRRGPPLH
                     PKPGKNCVLVQRRGQWQTKNCSKGKAHNFVCSRKL"
     exon            779..873
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1175
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            1176..1372
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1696
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1805
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1937
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2282
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            2283..2425
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            2426..2622
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            2623..2713
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            2714..2881
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            2882..3090
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            3091..3184
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            3185..3437
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            3438..3632
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            3633..3818
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            3819..4015
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            4016..4202
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            4203..4347
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            4348..4489
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            4490..4685
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            4686..4950
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            4951..5362
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            5363..5564
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            5565..5709
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            5710..5839
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            5840..5912
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            5913..6062
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            6063..6286
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            6287..6334
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            6335..6499
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            6500..6628
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            6629..6744
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            6745..6830
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
     exon            6831..9478
                     /gene="Frem1"
                     /gene_synonym="RGD1306981"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cagcggtact ggaggagccc tgcaaacgtg tttgggaact ccaattctct acctatttgt
       61 gttgtatggg ttaatggatt tttagtacaa acaaagccta aactcagctg tcttgaacga
      121 ctggaatttc agctctcctc cttggatggg atgtttttgc ttcctcttcc tctgaccact
      181 ctttaagcct gactaccaag ttggcgtcca gccttccact tccaaaggtg gtcagctctt
      241 ggcttggaaa ggaaaccact acctgattag gaaagcgctt gggaggcaca ttaatcttgc
      301 cagcgttgtc aggaaggatc agcgagtttt attaaaaagc cccgactgcc taaaaggcct
      361 ccttgtctga gtctgaatgg aaggggcatg ttaagatctt gggacatgct atcgggaccc
      421 ctggtcttgc cccctggacc tggagctgac actcggggag gaagtacaga aaccttgctc
      481 tgttgaacag actgtgctgc tggtggacag agctgaccct ggcatcatgc actctccagg
      541 ctgcactggg cccaaggctc tgttgttctt gctgctccag ctgcttatgc tacacctgga
      601 cccagccaat gccaccttca tcagcattaa ccggggcctg agggtgatga agggtagttc
      661 tgccttcctg tctggagatc acctgaggtt tgccatccct aaagagaagg acgcttgcag
      721 agtggaggtg gtgatgaatg aacccataac ccagagggtt gggaaactca ttccacaggt
      781 ctttgattgc cattttcttc ccaacgaagt acaatatgtt cacaatggat gcccaattct
      841 ggacgaagac tctgtgaagc ttagacttta caggtttact gaaacagaga ctttcatgga
      901 aaccttcctc ttgcgggtct ctcttgtgga accagactgt aacatcatcc gcctgagcag
      961 caacgtgttg gaagtgactg aattctatgg cttatcccaa gccattgata agaacctgct
     1021 ccagtttgat tatgatagga cagccagtct ggactgcacc atcagagtag accccgtgaa
     1081 gactcagctg ccagcccatg gcaagctggt tgtggtgaat cggaagtcag agggaccgcg
     1141 tggagaccag ccacacagct ttttctctga gtcacagcta ggggcagaac ggaagtgtcc
     1201 ggatggaagc tgtactctgg aaccgaagca agtgacaagt ctgaaagtga gctgtgagga
     1261 cttcctgctg acagggtttc actaccaaca catgcagccc ccttcaccca acattgacta
     1321 catccccatt cagttggatc tcacagacag aaggagtaaa accatttaca agtcagagag
     1381 cgcgtggttg cctgtcttca tcagagttgg cattcctaac caggccccaa gggctgcttt
     1441 catggccatg tttgttctgg aggtggacca gttcatattg acccctctga caacctccgt
     1501 gctggactgt gaggaggatg agactccgaa acccttgctg gtgtttaaca tcactgaagc
     1561 cccactccag ggctatgtga cccacctgct ggatcatacc agacccatct cttcatttac
     1621 ctggaaggat ctcagtgaca tgcaagttgc ctaccagcca ccgaacaaca gccaccctga
     1681 gaggagacat tacaagatgg agctggaggt gtacgacctc ttctttgaga ggagcgcccc
     1741 catcacagtt cacatctcca tcagaacagc agacacgaat gcacctcggg tgtcctggaa
     1801 tacaggtttg aatctcctag aggggcagtc tcgggccatc acttgggaac agtttcagat
     1861 cgtggacaat gatgacattg gtgctgtgca gttggtcacc attggtggtc tgcagcatgg
     1921 acggcttaca gtaagagagg ggaaaggatt tctcttcacc gtggctgatc ttcaggctgg
     1981 agttgttcgc tatcatcatg atgacagtga taccaccaaa gactttgtgg ctttcaggat
     2041 attcgatggc caacacagca gccatcacaa gtttcctatc aacatcttgc ctaaagatga
     2101 cagtccccca ttcctcataa ccaatgtcgt gattgaacta gaagagggga agaccatctt
     2161 gatccaaggg tccatgctga gagcttcaga catggactcc agtgatgact acatcttctt
     2221 caatatcaca aagttcccac aggctgggga gatcatgaag aagccagggc caagattgat
     2281 aggatatcct gtctctggct tccttcagag agacctgttc agtggaatca tttactatcg
     2341 tcactttggt ggagaaatct ttgaagattc ttttgaattt gtcctgtggg acagccatga
     2401 acctccaaat ctctcagtgc cacaggtggt gatggtccat ataactccag tagacgacca
     2461 gcttcccaag gaagctcccg gcatatcccg gcatttgatt gtcaaggaaa cggaagtggc
     2521 ctacatcact aaaaagcacc tacatttcct agacacggaa tcgcatggtg gggagcttgt
     2581 ctacacaata acacgccctc catgcttctc ctccagccac aggcacttgg atgctgggaa
     2641 gctgttcatg gtggacagca taccaaagcc aaccaagaat cccacagccc cagggctgaa
     2701 ctcatttact cagcatgctg tgaaccacat gaaagtggcc tacatgccac ccatgcagga
     2761 tataggtccc acacctcgac atgtgcagtt cacagtgtcc gtcagtaacc agcatggagg
     2821 tactttgcat ggaatctgct tcaacatcac agtgctccca gtggacaatc aggttcctga
     2881 ggtattcacc aacaccctga gagtggctga gggtggccaa tgcaccatca gcacagagca
     2941 cattttggtt tctgatgtgg acaccccact ggacagcatc tgcctctctc ttcaggagat
     3001 gcccctgcat ggcggggtag agctggatgg atttcctcta aacccaagag gcacattttc
     3061 ttggcgagac ctccatacct taaaagttag gtatcaacat gatggatctg aggttcttca
     3121 ggatgagata ttcttggagg tcacagacgg cacaaattct gcaggatttg ttctctacat
     3181 tgaggtattc cctgtgaatg atgagccacc tgttctaaag gctgacctca ttcccatgat
     3241 gcactgctca gagggtggag aggtgaccat cacccctgaa tacattttcg ctactgatgc
     3301 agacagcaat gactcagaac tgctatttct gattacccga gaacctcggc atggagtagt
     3361 gagaaaagct ggactccgtg tggacaggtt ctctcaggga gatgtcatct caggagctgt
     3421 ggcatacaaa catacaggtg gcgagattgg cctcgaatct tgttttgaca ctgttgtatt
     3481 ggtggtttcg gatggtgaag ctgacccttt ggtgaatggc tgttgctaca atggacccga
     3541 ttcatctgtt cctcttcata agtccttccc cacataccag ctcaacatca cggtgcatcc
     3601 agtcgacaat cagccacctt caattataat aggtaggacg ctcacggtgg acgaaggctt
     3661 ctcggcagcc cttacgactc atcacttgac tgccactgac tgggacactg ctccggatga
     3721 cttggaattc attttggttt ctcatcccca gtttggctac ctagaaaaca cactgccctc
     3781 tgcaggcttt gagaaaagga atacgggcat aagcatagct tcattccagt ggagagacat
     3841 gaaagcttcg catattaact atgtgcagtc caggcacctg agggaggagc caactgctga
     3901 tgagttcctg gtgtacgcca cagatgggaa gcaacgctcc ttggagacca catttcacgt
     3961 tatcatcaac cccacaaatg atgaagctcc tgattttgtg gtacagaata tcactgtgta
     4021 cgagggccac atggtagagc tggattctgc catcatcagt gctactgaca gggatgttcc
     4081 caaggacccc ttgctcttca acatcacgca caagcccaag catggcctcc tcctcatctc
     4141 ggccaccagc aaggatgctc ataatgaaat tcacaacttc tccatggaac tgctccagaa
     4201 tggaatgaag ctggcgtatg cccacgatga ctcggagagc tctgctgaca acttcgtgat
     4261 ccagttatca gacgggaaac ataaaatcct taaaaccgtt tcagtaagca tcaccccagt
     4321 gaacgacgag acaccaacac taagcaagaa ggctgaagtc tcagtgactg tgggcgacac
     4381 tcgtgttctg tctagtgctg ttctttcagc catagataaa gactcaccca gggagaagat
     4441 tcactactta tttgaaagac ttccccaaaa tgggcaactt caacttaaga tagggaggga
     4501 ctgggtccct ctctccgctg gcatgcagtg cacccaggag gatgtggact tgaacttgct
     4561 aagatacgcg catgctggta aaatgcattc ccaggatgga gaccgcttta ccttctacct
     4621 ctgggacggg gacaacaggt cacctgcatt tgactgtcac atcatcattg aagacacagg
     4681 aagaggtgat attgtcattc atgcgaaacc actcgtggta gccaacggtg acagaggtct
     4741 cttgacgcct gccactcttc tggctgagga tggagcagac aaggctgagg aattactcta
     4801 ccttatcact tccccaccac agtatggcca agtggagtat gtctactctc ctggagtccc
     4861 cattaccagc tttagccaaa tggacatagc aggacaggca gtctgctaca tacacaagag
     4921 caggaccgct gtcctcagtg acagtttcag atttaccatc agcaatggac tgcagaccca
     4981 gcagggggtg tttgagatca cactgcaggg tgtggacagt gccttgcctg tgctgaccag
     5041 gaaccgaagg ctgaggcttg tggaaggggc catgggcctg ctgtccacgg atcaccttca
     5101 gctgactgac cctgacacac ctccagagaa cctaaccttc tttctggtcc agctcccgcg
     5161 ccacgggtac ctctacctga gagggaaagt acttcagcac aacttcaccc agcaagatgt
     5221 agacagcagg gttgtggcct accagcatgc aggaggcggt gcccaggagg actgctttac
     5281 tttcctggcc acagacagga agaaccaagg cttcgttgtg gatgggaaag ttcagaaaga
     5341 gcccgttctc ttcattatcc aggtggacca gctggataag aaggcgcctc ggatcacacg
     5401 cttgcactcc ccttctcaag tggggctctt gaaaaatggc tgctatggga tttacatcac
     5461 ttcccatgtg ctaaaggcgt ctgaccctga cacagaggat gaccagatca tctttaagat
     5521 tttacgaggc ccgttgcatg gacgtctgga gaacacgaca acaggtgaat tcatccacga
     5581 gcaatttagc cagaaggact tgagccgcaa gaccatcctt tatatcataa acccttcttt
     5641 gcaagtgaat tcagatatcc tggaatttca ggccatggac cccacaggga acactgcccc
     5701 tccccagaga ttggaactga aatggtcata tattgaatgg tcccagactg aatatgaggt
     5761 ctgtgagaat gtgggcttac tgaccttgga agtcaccaga aggggatatt ccatggactc
     5821 agcctatgtg gacatagagg ttaaccaagt gtcagctgta gttggaaaag attttactgt
     5881 cactccatcc aaactgcttc aatttgaccc aggaatgtca actaagatgt ggaatatagc
     5941 aattacctat gacggattag aggaagacga tgaggtcttt gaagtaattc tgaactcccc
     6001 tgtgaatgcg gttcttggca cacagacaaa agcagcagtg aaaattttgg actcaaaagg
     6061 aggacggtgc catccttcaa attccttcaa ccaaagcaag cacagcacgt gggggaaggg
     6121 acgttggcat ccactgccct cagggtcatc ctcactcacc acttccggtt ctcctcatct
     6181 ggaaagaagg ccccctccat ccatcaccaa aggagatgct cttcggggtt ttgatctcac
     6241 agacctctct caaaggaaga tgacgacaca ggggaatggc aaatcagtcc tcccatcctc
     6301 tgtttgtaga aatggaacag acaccatcta caattatcat ggtatagttt ccttgaaact
     6361 ggagggggac agattctcag ctcacaaacg gaaggccaaa atatccgtca ttcgtcagcc
     6421 acagaggaca gtcaaggtgg cagagctgcc tctagccgat aaggtagaac ccacaaccga
     6481 ctcgaacttt ctcagtcagg gtctacggcc cttgtttcca aagaactgtt ctgtggaatt
     6541 aaagggactc tttcatttgg aagaacacac ccacagattg tatcaatgtg atgggatcgc
     6601 ctggaacgtc tggagctccc aaaccaaggg gctggaggac agatcctgcc cagttggatg
     6661 gcttcttcat tccgactact gtcacgtctt ggtcacaagg cagaaaggca cctgggccac
     6721 agctacccga gcttgcagag aacagcacca gggcaccctt gtgaccgtat tctccaggag
     6781 gcacatgcag tggctgtggg ctatcagcgg aagaaagccc ttctggatag gcttgaagaa
     6841 ccaagtgcga actggccact gggagtggat tggaggtgaa cctgttgctt tcaccaactg
     6901 gaggagaggg ccccctctgc acccgaagcc tggaaagaac tgtgttttgg ttcaaagaag
     6961 aggacaatgg caaacaaaga actgtagcaa aggcaaggct cacaattttg tgtgctcaag
     7021 gaaactctga acggaacccg agatttctca tctgtctatt tacaagatct acgagtattg
     7081 ttctgtggaa atgcattgct gtggctgact ggccatcatg cctttgtgag tctgtcaagt
     7141 aaacatggaa actagaaaaa cagtcccagg gagatagctg cgtgaatgtg cctttcaaga
     7201 tgagatacaa cttctgtgtc tcaatatttt tctggataag tgtctgcagt atgataggct
     7261 ttagtatttg cttcttctag aagattcaga cttattccta tgccagctct caggcagtac
     7321 gagtttccag ggaccttaaa tgcttttgtc tgtagacctc gaacgagtcc atccggaaag
     7381 atcagtgttg cagtgcttcc accacagtct gagtgaacct agccctgccg agccattaaa
     7441 ccaaggtcga atgccaaagg ttggtttgtt ttcttaggtc ctttaaatgt gaacttaata
     7501 ccagaggctg tgtatagaca aaggggctct gtttgaaatg gaagagtctc tcaggaggat
     7561 acttaactga ataggtctag tgggatgggg cagaggcctg cctttattgg agggctcaga
     7621 tgcaggcatg gctctgcctc tgctaaggag atcaaaccat tccactcatg agacgtgtta
     7681 catctcagtt caatcgtggc atcgtgccat tcctaaatga taggttttcc catcttccaa
     7741 ggtcacgtga actttgaagt agcaatattt tcatctctgt ttacaatttt gaccaaatat
     7801 ttttaaagga tatgtggaat aaggagtgta tatgactatg gttgacataa agaagaaaaa
     7861 ggccactgtc tcagaagaac cctcatgaac tgttctgatg accaaagaaa aataatactg
     7921 gaagcagacc ctttgaaagt ccacaccacg ctcttggaaa ggattttgat gggactttga
     7981 gactttacat ctctggaatt tgcagggatt ctctgctaat tagattctcc aatagaaggt
     8041 gccttattaa tttataaact cagttgagtg gaacatttgt agcaatgaag catgctgtgc
     8101 gtctctcaac aatcccttct ggaacccagg gttaattcta ggaagttagg acgtcctttt
     8161 tgaaatgttt aataaatttt tttcttttca taatctctaa aaaatacagc tgtgccaaat
     8221 ataacttgtc acatgagtac cggtgtaact ttagaattaa atgaggtgtt caagtagact
     8281 agggctttag ggcagacttt ttaaacagaa taataatatc acaagtgtta cagacactaa
     8341 aaatggctta agaaagtcgt tccagtttct ggatataaaa catgacaggc ttgcttcatt
     8401 tatcaaagtt ttggttcata gacattttta cctaaggagg ttaggataag actcagacat
     8461 tctgtctctt attgaaatcc agatgtaatt gcagagctgg ctgctcttag ctcggaggtt
     8521 tggaggagtc tgtagacagt aaatacaaag acccgtggaa cataaggatc ctttagtgtt
     8581 taacgaaaac tggccaaatc cttcctccac tccagttcca gccaagttcc ttgtaatcca
     8641 ctagcttcct caccgtacaa agttaggatt cagactgtgg gaaagaggat ggagttctcc
     8701 cattcagata ttgaggaatt cattctaaag ctgcgtgcac cattaaagct ggaaatacag
     8761 agctatgcca accatgctca acctgggtgg caaggccagt ttattagact ttcttgcact
     8821 gaaaatggca tggccgtaat tgcaagtggt taacataggg gatggcttgg gctttatatc
     8881 cattaggaat agatcattct gaaattttct tgacatgaaa gctttatcaa agtgagacat
     8941 caggtctaag tgattagaag aacacacaag ttcaggcaag cttaccttct ggccacgact
     9001 gtcagggttg agtagggagt gctggtgatg ccagtagcca agcagggtat acagcagagg
     9061 tccgactgta gtcagcccat gccctctaac ttgaatgtcc actcaggaac ttcctacatg
     9121 catggacata cctgcagctc aatgctttag aacttttaaa tttttttctg atatttgatc
     9181 tttaaaagga tacatagtat atgtttactg aagataaatc ttaaaagctg gccctaatca
     9241 tttcctcctc ttccctggag tggtgaaaac aaactgagca atgtttattt gtatttctag
     9301 tctagtttat ttttaataca gcccaaatat gcatatattt taatgaacac tgtggtcctt
     9361 tgtcatttaa atcatcgact ggtgctaatt tctgtaagga ggtagtatat cagccgtact
     9421 gtatctagtg ttgtgtcaaa gccaataaaa taaacatgtt taatcctcag acactgta
//
