LOCUS       NM_001146125            2586 bp    mRNA    linear   ROD 23-MAR-2023
DEFINITION  Rattus norvegicus folylpolyglutamate synthase (Fpgs), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_001146125 XM_001078256 XM_001078273
VERSION     NM_001146125.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Lawrence SA, Titus SA, Ferguson J, Heineman AL, Taylor SM and Moran
            RG.
  TITLE     Mammalian mitochondrial and cytosolic folylpolyglutamate synthetase
            maintain the subcellular compartmentalization of folates
  JOURNAL   J Biol Chem 289 (42), 29386-29396 (2014)
   PUBMED   25164808
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Schneider E and Ryan TJ.
  TITLE     Gamma-glutamyl hydrolase and drug resistance
  JOURNAL   Clin Chim Acta 374 (1-2), 25-32 (2006)
   PUBMED   16859665
  REMARK    Review article
REFERENCE   3  (bases 1 to 2586)
  AUTHORS   Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl
            E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES and
            Mann M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   4  (bases 1 to 2586)
  AUTHORS   Galivan J, Ryan TJ, Chave K, Rhee M, Yao R and Yin D.
  TITLE     Glutamyl hydrolase. pharmacological role and enzymatic
            characterization
  JOURNAL   Pharmacol Ther 85 (3), 207-215 (2000)
   PUBMED   10739875
  REMARK    Review article
REFERENCE   5  (bases 1 to 2586)
  AUTHORS   Barredo J and Moran RG.
  TITLE     Determinants of antifolate cytotoxicity: folylpolyglutamate
            synthetase activity during cellular proliferation and development
  JOURNAL   Mol Pharmacol 42 (4), 687-694 (1992)
   PUBMED   1435744
REFERENCE   6  (bases 1 to 2586)
  AUTHORS   Bolanowska WE, Russell CA and McGuire JJ.
  TITLE     Activation of mammalian folylpolyglutamate synthetase by sodium
            bicarbonate
  JOURNAL   Arch Biochem Biophys 281 (2), 198-203 (1990)
   PUBMED   2168155
REFERENCE   7  (bases 1 to 2586)
  AUTHORS   Clarke,L. and Waxman,D.J.
  TITLE     Human liver folylpolyglutamate synthetase: biochemical
            characterization and interactions with folates and folate
            antagonists
  JOURNAL   Arch Biochem Biophys 256 (2), 585-596 (1987)
   PUBMED   3619447
REFERENCE   8  (bases 1 to 2586)
  AUTHORS   Tolomelli B, Marchi Marchetti M, Laffi R and Marchetti M.
  TITLE     Folylpolyglutamate synthetase activity in regenerating rat liver
  JOURNAL   Int J Vitam Nutr Res 57 (2), 169-172 (1987)
   PUBMED   3654111
REFERENCE   9  (bases 1 to 2586)
  AUTHORS   McGuire,J.J., Hsieh,P., Coward,J.K. and Bertino,J.R.
  TITLE     In vitro methotrexate polyglutamate synthesis by rat liver
            folylpolyglutamate synthetase and inhibition by bromosulfophthalein
  JOURNAL   Adv Exp Med Biol 163, 199-214 (1983)
   PUBMED   6193685
REFERENCE   10 (bases 1 to 2586)
  AUTHORS   McGuire,J.J., Hsieh,P., Coward,J.K. and Bertino,J.R.
  TITLE     Enzymatic synthesis of folylpolyglutamates. Characterization of the
            reaction and its products
  JOURNAL   J Biol Chem 255 (12), 5776-5788 (1980)
   PUBMED   6892914
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CH474001.2.
            
            On Apr 3, 2009 this sequence version replaced NM_001146125.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMEA5760400
                              [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3p11"
     gene            1..2586
                     /gene="Fpgs"
                     /note="folylpolyglutamate synthase"
                     /db_xref="GeneID:687266"
                     /db_xref="RGD:1587713"
     exon            1..164
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..1790
                     /gene="Fpgs"
                     /EC_number="6.3.2.17"
                     /note="folylpolyglutamate synthase, mitochondrial"
                     /codon_start=1
                     /product="folylpolyglutamate synthase, mitochondrial
                     precursor"
                     /protein_id="NP_001139597.2"
                     /db_xref="GeneID:687266"
                     /db_xref="RGD:1587713"
                     /translation="MSWARSHLCSALSLAAVSARGATMEGAAYRWLSAWPAPQEPSME
                     YQDAVRTLNTLQTNASCLEEVKRQRSDPQAQLEAMEVYLARSGLQVEDLNQLKIIHVT
                     GTKGKGSTCAFTERILRNYGLKTGFFSSPHLVQVRERIRINGKPISPELFTKYFWHLY
                     QQLEEFKDDSHISMPSYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAFDCTNIIRKPVV
                     CGVSSLGIDHTSLLGDTVEKIAWQKGGIFKPGVPAFTVLQPEGPLAVLRDRAQQISCP
                     LYLCPPLEALEQVGLPLSLGLEGEHQRHNAALALQLARCWLEQQNYHDIQELKASKPS
                     IRWQLPLAPVFRPTPHMRRGLRDTEWPGRTQVLQRGPVTWYLDGAHTTSSVQACVHWY
                     CQSLERSRRTDGGHEVHILLFNSTGDRDSAALLKLLQPCHFDYAVFCPNVTEISSIGN
                     ADQQNFTVTLDQVLLRCLQHQKHWNCLAEKQASSNLWGSCGPESARPGSLLLAPHPPQ
                     PTSTSSLVFSCISHALQWISQGRDPIFQPESPPRNLLNHPTANSGASILREAAAIHVL
                     VTGSLHLVGGALRLLDPSMSQ"
     exon            165..293
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            294..347
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            348..412
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            413..527
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            528..605
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            606..667
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            668..770
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            771..848
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            849..996
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1086
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1237
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1313
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1380
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..2586
                     /gene="Fpgs"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gcctagcttg cagtggcatt aaggctatgt cgtgggcgcg gagccacctg tgctcggctc
       61 tatccttggc agctgtttct gcgcgtggtg caacgatgga gggcgcggcg tatcggtggt
      121 tgagcgcgtg gccagcgccg caggagccga gcatggagta tcaggatgct gtgcgcacgc
      181 tcaacaccct gcaaaccaat gccagctgcc tggaggaggt aaagcgtcaa cggagtgacc
      241 ctcaggcgca gctagaagct atggaggtgt acctcgcacg gagtggactg caggtggagg
      301 atttgaacca actaaaaatt atccatgtca ctgggaccaa aggaaagggc tccacctgtg
      361 ccttcactga acggatcctg cggaattacg gcctgaaaac tggcttcttt agctctcccc
      421 acctggtgca ggtgcgtgag cggattcgaa tcaacgggaa gcccatcagc cccgaactct
      481 ttaccaagta cttctggcac ctctatcagc agctggagga attcaaggat gacagccaca
      541 tttccatgcc ctcttacttc cgcttcctca cactcatggc tttccatgtc ttcctccaag
      601 agaaggtgga cctggccgtg gtggaagtgg gcattggtgg ggcttttgac tgcaccaaca
      661 tcatcagaaa gccagtggtg tgtggagtct cctctcttgg cattgaccac accagtctgc
      721 taggagatac agtggagaaa atagcatggc agaaaggggg catctttaag cctggtgtcc
      781 ctgccttcac tgtgctacag ccagaaggtc ccctggccgt gctgagggat agagcccagc
      841 agatctcatg ccccctgtac ctgtgtccac cattggaagc cctggagcag gttggattgc
      901 cattgagcct gggcctggag ggagagcacc agcggcataa tgcagccttg gccttgcagc
      961 tggcccgttg ttggctggag cagcagaact accatgacat ccaggagctg aaggcatcca
     1021 agccaagcat tcggtggcag ctgccgctgg cacctgtgtt ccgccccacg ccccacatga
     1081 gacgtgggct tcgggacaca gagtggcctg gtcggacaca ggtactccag cggggacctg
     1141 ttacctggta cctggatggc gctcatacca ccagcagtgt gcaggcctgt gtgcactggt
     1201 actgccagtc actggagcgc agcagacgca ccgacggtgg gcatgaagta cacatcttgc
     1261 tcttcaactc cactggtgac agggactctg ctgccctact gaagttgctg cagccctgcc
     1321 attttgatta cgctgtcttc tgccccaatg tgacagagat ttcatccata ggaaacgcag
     1381 accagcagaa cttcacggtg actctggacc aagtgctcct ccgctgcctg cagcaccaga
     1441 agcattggaa ctgcctggca gagaaacagg ccagctccaa cctctggggc agctgtggcc
     1501 cagagtctgc ccggccaggc tccctgctgc tggcaccgca cccacctcag cccactagca
     1561 cgagctccct tgttttcagc tgcatctccc acgccttgca gtggatcagc caaggccggg
     1621 atcccatctt tcagcccgag agccctccaa ggaaccttct gaaccacccc acggccaaca
     1681 gcggggcgag cattctccga gaggccgctg ccatccacgt actggtcacg gggagcctgc
     1741 acctggtggg tggggcgctg aggctgcttg atccctccat gtctcagtag ccaaggagcg
     1801 tcctacacca gcctgccttc ccacagagac tctcatactc agtgccttgt gatttctgct
     1861 ctcagattct tcgagactgg cccagggtcc tgggctcttg gtagagtgta gggatgggag
     1921 gggctctgtg ccttggcctc tcctttctct ggcagagaca gcaggggctt cccagagccc
     1981 ctaccatcag cccagcccag ctccctgccc actctgctgc ctccgggctc aggtccagct
     2041 tattgttgga gctgcctgag gttagaccag agacttcctt cttcctccca atgccttctg
     2101 gccaaagaca gggcccttgc ctgggcattt ggggactggg tgttgctgga actaattgaa
     2161 gcttttaaac ccttttattt tttatttttt ttaaataaat gacaaaactt ttgattgatt
     2221 cctaaccgcc cagctccctc atcagacatt tgggggctgg agtttggcac tgcaggagtg
     2281 taggaaggaa tggagcccta gcagtatagc ctgggattag tcctctccct cagagcataa
     2341 ggtggcctgt gtcaggaaag actggactaa tgtttatgtg ccacacacca aggcaaacct
     2401 cctggccaat aatgtgtcca agctggatgc ccaccttacg gatgagggag ggtggccggc
     2461 ttccaggagc tttgggcctg gacccataga cccattgcca acaagagtca tgtgcaggat
     2521 tagaacccag agctttatag gggtagctac gtcccggacc ccagagacta tacacagggc
     2581 ttttta
//
