LOCUS       NM_144561               2278 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus flavin containing dimethylaniline monoxygenase 4
            (Fmo4), transcript variant 2, mRNA.
ACCESSION   NM_144561
VERSION     NM_144561.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Novick,R.M., Mitzey,A.M., Brownfield,M.S. and Elfarra,A.A.
  TITLE     Differential localization of flavin-containing monooxygenase (FMO)
            isoforms 1, 3, and 4 in rat liver and kidney and evidence for
            expression of FMO4 in mouse, rat, and human liver and kidney
            microsomes
  JOURNAL   J Pharmacol Exp Ther 329 (3), 1148-1155 (2009)
   PUBMED   19307449
  REMARK    GeneRIF: Data show a compelling visual demonstration of the
            isoform-specific localization patterns of FMO1, -3, and -4 in the
            rat liver and kidney and the first evidence for expression of FMO4
            at the protein level in mouse and human liver and kidney
            microsomes.
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Hernandez,D., Janmohamed,A., Chandan,P., Omar,B.A., Phillips,I.R.
            and Shephard,E.A.
  TITLE     Deletion of the mouse Fmo1 gene results in enhanced pharmacological
            behavioural responses to imipramine
  JOURNAL   Pharmacogenet Genomics 19 (4), 289-299 (2009)
   PUBMED   19262426
REFERENCE   3  (bases 1 to 2278)
  AUTHORS   Lattard,V., Longin-Sauvageon,C. and Benoit,E.
  TITLE     Cloning, sequencing and tissue distribution of rat
            flavin-containing monooxygenase 4: two different forms are produced
            by tissue-specific alternative splicing
  JOURNAL   Mol Pharmacol 63 (1), 253-261 (2003)
   PUBMED   12488558
  REMARK    GeneRIF: By Western blotting using the two different forms of FMO4
            antibodies, a long FMO4 protein was detected in the rat kidney,
            whereas in the rat brain, only the short form of FMO4 was observed.
REFERENCE   4  (bases 1 to 2278)
  AUTHORS   McCombie,R.R., Dolphin,C.T., Povey,S., Phillips,I.R. and
            Shephard,E.A.
  TITLE     Localization of human flavin-containing monooxygenase genes FMO2
            and FMO5 to chromosome 1q
  JOURNAL   Genomics 34 (3), 426-429 (1996)
   PUBMED   8786146
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On Mar 15, 2021 this sequence version replaced NM_144561.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF458416.1, BC081721.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-186               JAXUCZ010000013.1  77705915-77706100   c
            187-313             JAXUCZ010000013.1  77704734-77704860   c
            314-453             JAXUCZ010000013.1  77702344-77702483   c
            454-642             JAXUCZ010000013.1  77700804-77700992   c
            643-805             JAXUCZ010000013.1  77700100-77700262   c
            806-948             JAXUCZ010000013.1  77697179-77697321   c
            949-1145            JAXUCZ010000013.1  77695612-77695808   c
            1146-1498           JAXUCZ010000013.1  77693123-77693475   c
            1499-1568           JAXUCZ010000013.1  77689305-77689374   c
            1569-2278           JAXUCZ010000013.1  77687909-77688618   c
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q22"
     gene            1..2278
                     /gene="Fmo4"
                     /note="flavin containing dimethylaniline monoxygenase 4"
                     /db_xref="GeneID:246247"
                     /db_xref="RGD:628601"
     exon            1..186
                     /gene="Fmo4"
                     /inference="alignment:Splign:2.1.0"
     exon            187..313
                     /gene="Fmo4"
                     /inference="alignment:Splign:2.1.0"
     exon            314..453
                     /gene="Fmo4"
                     /inference="alignment:Splign:2.1.0"
     CDS             322..2004
                     /gene="Fmo4"
                     /EC_number="1.14.13.8"
                     /note="long isoform is encoded by transcript variant 2;
                     dimethylaniline monooxygenase [N-oxide-forming] 4; FMO 4;
                     dimethylaniline oxidase 4; hepatic flavin-containing
                     monooxygenase 4; flavin containing monooxygenase 4"
                     /codon_start=1
                     /product="dimethylaniline monooxygenase [N-oxide-forming]
                     4 long isoform"
                     /protein_id="NP_653147.2"
                     /db_xref="GeneID:246247"
                     /db_xref="RGD:628601"
                     /translation="MAKKVAVIGAGVSGLSSIKCCLDENLEPTCFERSSDFGGLWKFA
                     EASEDGMTRVYRSLVTNVCKEMSCYSDFPFHEDYPNFMSHEKFWDYLREFAEHFGLLK
                     YIRFKTTVRSVTKRPDFSETGQWEVVTETEGKQDRAVFDAVMVCTGQFLSPRLPLESF
                     PGIHKFKGQILHSQEYRIPDAFRGKRILVVGLGNTGGDVAVELSGIAAQVFLSTRTGA
                     WVRSRSSVGGYPLNMMQTRWRNFLAQVLPSRFVSWNQERQMNKIFNHENYGLSIAKGK
                     KPKFIVNDELPTCILCGKITMKTSVKDFTESSIVFEDGTIEANIDVVIFTTGYEFSFP
                     FFEEPLKSLCTKKVILYKRVFPPNLERSTLAIIGLISLTGSILVGTEFQARWATRVFK
                     GLCNIPPSQKLMAEAIKKEELIKRGVIKDTSQDKLDFISYMDELTQCIGAKPNIPLLF
                     LKDPRLAWEVFFGPCTPYQYRLMGPGRWDGARNAILTQWDRTVKPLKTRTVPKSQEPA
                     SLSRYLKTWGAPVLIVSLLLIYKSSLFLELVQSKLQGRFSPSRILWYIPQNS"
     exon            454..642
                     /gene="Fmo4"
                     /inference="alignment:Splign:2.1.0"
     exon            643..805
                     /gene="Fmo4"
                     /inference="alignment:Splign:2.1.0"
     exon            806..948
                     /gene="Fmo4"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1145
                     /gene="Fmo4"
                     /inference="alignment:Splign:2.1.0"
     exon            1146..1498
                     /gene="Fmo4"
                     /inference="alignment:Splign:2.1.0"
     exon            1499..1568
                     /gene="Fmo4"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..2278
                     /gene="Fmo4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atagcagaag aagcaacggg cttttgtgtt gaactgagag tttgagcaaa gtcagcaaag
       61 tcagtagcac tctgtttcct ccttaggaca aggctctgag accgaaactt cttcctgttg
      121 ttttataaaa caaactccac ctttgacctc tcacctttgg cttctcacct gctctatgct
      181 tgcaagacag ggcacggcgg agatccgaca acttcagacc ttcacccaga aattcaagtt
      241 gttcttctcg gtgaaggccg tttgtccctg gttgaatgcc acccaggtgt tgacaaccca
      301 agggaatcca cagagcgcac catggccaag aaagtggcag tgattggagc tggagtgagt
      361 ggcctgtcct caatcaagtg ctgcttggac gagaacctgg agcctacctg ttttgagagg
      421 agtagtgact tcggcggact gtggaagttt gctgaagcat ctgaggatgg aatgaccagg
      481 gtttacaggt cactagtgac aaatgtctgc aaggaaatgt catgttacag tgacttccca
      541 ttccacgaag attaccccaa tttcatgagc catgagaaat tctgggacta tctcagagaa
      601 tttgctgagc acttcggcct cctgaaatat attcggttta agactactgt gcggagtgta
      661 actaagcgtc cagacttctc tgaaactggc caatgggaag ttgtcacaga gacagagggc
      721 aaacaggaca gagctgtatt tgatgctgtt atggtttgca ctgggcaatt cctgagccca
      781 cgtttacctc tggagtcctt ccctggaatc cataagttta aaggacagat cctacacagc
      841 caagagtaca ggattccaga tgcctttcgg ggcaagcgca tcctggtggt tggtcttgga
      901 aacactggcg gggatgtcgc agtggagctc agtggaatag cagcacaggt gtttctcagc
      961 actaggactg gtgcctgggt tcgcagccgc tcctcagttg gtggctaccc tttgaatatg
     1021 atgcagacaa gatggcgcaa cttcctcgca caagttctgc cctcccgctt tgtaagctgg
     1081 aatcaagaga ggcaaatgaa taaaatattt aaccatgaga actatggatt aagcattgca
     1141 aaagggaaaa aaccaaaatt tattgtgaat gatgaactac caacttgtat cctctgtggg
     1201 aaaatcacca tgaaaaccag cgtgaaggat tttacagaat cctctatcgt ctttgaagat
     1261 gggacaatag aagccaacat tgatgtagtg atcttcacta caggatatga attttctttt
     1321 cccttttttg aagagcctct caaaagcctt tgcacaaaga aggtcatact atacaagcga
     1381 gtgtttcccc caaatttgga gagatcaaca ttagccatca ttggcctgat cagccttact
     1441 gggtctatct tagtgggcac agagttccaa gcacggtggg ccacgagagt attcaaaggg
     1501 ctctgtaata tacctccatc tcagaaactg atggccgaag ccattaaaaa ggaagagctc
     1561 attaaaaggg gtgtgattaa agataccagc caagacaaac tcgacttcat ttcctacatg
     1621 gatgagctca ctcagtgcat aggcgcgaag cccaatattc cacttctgtt ccttaaggac
     1681 ccaagattgg cgtgggaagt tttctttgga ccatgtaccc cttaccaata tcgcctaatg
     1741 ggccctggaa gatgggacgg agccagaaat gccatcctga cccagtggga caggactgtg
     1801 aaaccactaa agactcgaac tgttcctaag tcccaggagc ctgcctccct ctcacgttac
     1861 ttaaaaactt ggggggcacc tgtcctgatt gtctctcttt tacttatcta caagtcttca
     1921 ctgtttcttg aactggtgca aagtaagctg caaggaaggt tttccccttc ccgaatactg
     1981 tggtacatcc cccagaactc atgagttggt ctccagtttg gcaatggagg aagttatctg
     2041 ggacatccct taaacagact agtgctgctc tcaaacactg gatcagtctc catagggcag
     2101 gttgtcataa agcaaggctg cctcttatcg ctttttcatg catttatttg tgccctttga
     2161 cctttctact atgtaatgac atagtacaaa cctctcacca gaagctgcca ctgtgtcctt
     2221 gagccctcca gcctccagaa tcatgaacaa aataaacttc atttacccat tttatgta
//
