LOCUS       NM_001270800            2653 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus flotillin 2 (Flot2), transcript variant 2, mRNA.
ACCESSION   NM_001270800
VERSION     NM_001270800.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Kessler,E.L., van Stuijvenberg,L., van Bavel,J.J.A., van
            Bennekom,J., Zwartsen,A., Rivaud,M.R., Vink,A., Efimov,I.R.,
            Postma,A.V., van Tintelen,J.P., Remme,C.A., Vos,M.A., Banning,A.,
            de Boer,T.P., Tikkanen,R. and van Veen,T.A.B.
  TITLE     Flotillins in the intercalated disc are potential modulators of
            cardiac excitability
  JOURNAL   J Mol Cell Cardiol 126, 86-95 (2019)
   PUBMED   30452906
  REMARK    GeneRIF: the presence of Flotillin-1, in addition to Flotillin-2 in
            the cardiac intercalated disc, is reported.
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Bodrikov,V., Pauschert,A., Kochlamazashvili,G. and Stuermer,C.A.O.
  TITLE     Reggie-1 and reggie-2 (flotillins) participate in Rab11a-dependent
            cargo trafficking, spine synapse formation and LTP-related AMPA
            receptor (GluA1) surface exposure in mouse hippocampal neurons
  JOURNAL   Exp Neurol 289, 31-45 (2017)
   PUBMED   27993509
  REMARK    Erratum:[Exp Neurol. 2017 Jul;293:200. doi:
            10.1016/j.expneurol.2017.02.016.. PMID: 28292530]
REFERENCE   3  (bases 1 to 2653)
  AUTHORS   Luo,Y., Akama,T., Okayama,A., Yoshihara,A., Sue,M., Oda,K.,
            Hayashi,M., Ishido,Y., Hirano,H., Hiroi,N., Katoh,R. and Suzuki,K.
  TITLE     A Novel Role for Flotillin-Containing Lipid Rafts in
            Negative-Feedback Regulation of Thyroid-Specific Gene Expression by
            Thyroglobulin
  JOURNAL   Thyroid 26 (11), 1630-1639 (2016)
   PUBMED   27676653
  REMARK    GeneRIF: Data suggest that flotillin-1 and flotillin-2 function as
            thyroglobulin-binding proteins in lipid rafts of thyrocytes; these
            interactions appear to exert negative-feedback effects on gene
            expression in thyrocytes.
REFERENCE   4  (bases 1 to 2653)
  AUTHORS   Possidonio,A.C., Soares,C.P., Portilho,D.M., Midlej,V.,
            Benchimol,M., Butler-Browne,G., Costa,M.L. and Mermelstein,C.
  TITLE     Differences in the expression and distribution of flotillin-2 in
            chick, mice and human muscle cells
  JOURNAL   PLoS One 9 (8), e103990 (2014)
   PUBMED   25105415
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2653)
  AUTHORS   Fork,C., Hitzel,J., Nichols,B.J., Tikkanen,R. and Brandes,R.P.
  TITLE     Flotillin-1 facilitates toll-like receptor 3 signaling in human
            endothelial cells
  JOURNAL   Basic Res Cardiol 109 (6), 439 (2014)
   PUBMED   25204797
REFERENCE   6  (bases 1 to 2653)
  AUTHORS   Neumann-Giesen,C., Falkenbach,B., Beicht,P., Claasen,S., Luers,G.,
            Stuermer,C.A., Herzog,V. and Tikkanen,R.
  TITLE     Membrane and raft association of reggie-1/flotillin-2: role of
            myristoylation, palmitoylation and oligomerization and induction of
            filopodia by overexpression
  JOURNAL   Biochem J 378 (Pt 2), 509-518 (2004)
   PUBMED   14599293
  REMARK    GeneRIF: Myristoylation and palmitoylation of flotillin-2 are
            necessary for raft membrane association.
REFERENCE   7  (bases 1 to 2653)
  AUTHORS   Rouvinski,A., Gahali-Sass,I., Stav,I., Metzer,E., Atlan,H. and
            Taraboulos,A.
  TITLE     Both raft- and non-raft proteins associate with CHAPS-insoluble
            complexes: some APP in large complexes
  JOURNAL   Biochem Biophys Res Commun 308 (4), 750-758 (2003)
   PUBMED   12927782
REFERENCE   8  (bases 1 to 2653)
  AUTHORS   Vorbach,C., Harrison,R. and Capecchi,M.R.
  TITLE     Xanthine oxidoreductase is central to the evolution and function of
            the innate immune system
  JOURNAL   Trends Immunol 24 (9), 512-517 (2003)
   PUBMED   12967676
  REMARK    Review article
REFERENCE   9  (bases 1 to 2653)
  AUTHORS   Lang,D.M., Lommel,S., Jung,M., Ankerhold,R., Petrausch,B.,
            Laessing,U., Wiechers,M.F., Plattner,H. and Stuermer,C.A.
  TITLE     Identification of reggie-1 and reggie-2 as
            plasmamembrane-associated proteins which cocluster with activated
            GPI-anchored cell adhesion molecules in non-caveolar micropatches
            in neurons
  JOURNAL   J Neurobiol 37 (4), 502-523 (1998)
   PUBMED   9858255
REFERENCE   10 (bases 1 to 2653)
  AUTHORS   Bickel,P.E., Scherer,P.E., Schnitzer,J.E., Oh,P., Lisanti,M.P. and
            Lodish,H.F.
  TITLE     Flotillin and epidermal surface antigen define a new family of
            caveolae-associated integral membrane proteins
  JOURNAL   J Biol Chem 272 (21), 13793-13802 (1997)
   PUBMED   9153235
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CB581049.1 and BC083550.1.
            
            Transcript Variant: This variant (2) has an alternate coding exon
            of the same size in place of an internal coding exon compared to
            variant 1. The resulting isoform (2) is the same size as isoform 1
            but has a different internal segment compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF023302.1, SRR8487231.80177.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-608               CB581049.1         1-608
            609-2653            BC083550.1         485-2529
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="10"
                     /map="10q25"
     gene            1..2653
                     /gene="Flot2"
                     /note="flotillin 2"
                     /db_xref="GeneID:83764"
                     /db_xref="RGD:70993"
     exon            1..152
                     /gene="Flot2"
                     /inference="alignment:Splign:2.1.0"
     CDS             104..1390
                     /gene="Flot2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     REG-1; reggie-1; reggie1-1"
                     /codon_start=1
                     /product="flotillin-2 isoform 2"
                     /protein_id="NP_001257729.1"
                     /db_xref="GeneID:83764"
                     /db_xref="RGD:70993"
                     /translation="MGNCHTVGPNEALVVSGGCCGSDYKQYVFGGWAWAWWCISDTQR
                     ISLEIMTLQPRCEDVETAEGVALTVTGVAQVKIMTEKELLAVACEQFLGKNVQDIKNV
                     VLQTLEGHLRSILGTLTVEQIYQDRDQFAKLVREVAAPDVGRMGIEILSFTIKDVYDK
                     VDYLSSLGKTQTAVVQRDADIGVAEAERDAGIREAECKKEMLDVKFMADTKIADSKRA
                     FELQKSAFSEEVNIKTAEAQLAYELQGAREQQKIRQEEIEIEVVQRKKQIAVEAQEIL
                     RTDKELIATVRRPAEAEAHRIQQIAEGEKVKQVLLAQAEAEKIRKIGEAEAAVIEAMG
                     KAEAERMKLKAEAYQKYGDAAKMALVLEALPQIAAKISAPLTKVDEIVVLSGDNSKVT
                     SEVNRLLAELPASVHALTGVDLSKIPLIKNATGAQV"
     exon            153..234
                     /gene="Flot2"
                     /inference="alignment:Splign:2.1.0"
     exon            235..325
                     /gene="Flot2"
                     /inference="alignment:Splign:2.1.0"
     exon            326..449
                     /gene="Flot2"
                     /inference="alignment:Splign:2.1.0"
     exon            450..568
                     /gene="Flot2"
                     /inference="alignment:Splign:2.1.0"
     exon            569..682
                     /gene="Flot2"
                     /inference="alignment:Splign:2.1.0"
     exon            683..802
                     /gene="Flot2"
                     /inference="alignment:Splign:2.1.0"
     exon            803..1017
                     /gene="Flot2"
                     /inference="alignment:Splign:2.1.0"
     exon            1018..1201
                     /gene="Flot2"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1351
                     /gene="Flot2"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..2636
                     /gene="Flot2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ggctggggga ggcgggatag gctgggcgcg gccggtgctg cggacccgcc acagccctcc
       61 aggtcgctgc ggacccaagg cctgcttgcc tgccagcggc accatgggca attgccacac
      121 ggtgggcccc aacgaggcac tggtggtctc agggggctgt tgtggttctg actacaagca
      181 gtatgtgttt ggcggctggg cttgggcctg gtggtgtatc tcggacactc agaggatttc
      241 cctagagatt atgacgttgc agccccgctg tgaggacgta gagacggccg agggggtagc
      301 tttaactgtg acgggtgtcg cccaggtgaa gatcatgacg gagaaggagc tcctggctgt
      361 agcctgtgaa cagttcctgg gcaagaacgt gcaggacatt aagaacgtcg tactgcagac
      421 cctggagggg catctacgct ccatccttgg gactctgact gtggagcaga tttatcagga
      481 ccgagaccag tttgccaagc tggtgcggga agtggcagcc cctgatgttg gccgtatggg
      541 catcgagatc ctcagcttca ccatcaagga tgtctatgac aaagtagact atctgagctc
      601 cctgggcaag acacagactg ccgtggtaca gagagatgca gacatcggtg tggcagaggc
      661 agagcgggac gcaggcatcc gggaagccga gtgcaagaag gaaatgctag atgtgaagtt
      721 catggcagac accaagattg ctgactccaa gagagccttt gagctgcaaa agtcagcctt
      781 cagtgaggag gtcaacatca agacagctga ggcccagttg gcctatgagc tacaaggggc
      841 cagagagcaa cagaagatcc ggcaggaaga gattgagatt gaggtagtac agcgcaagaa
      901 gcagatcgcc gtggaggcgc aggagatcct gcgcacagac aaggagctca tcgccacagt
      961 gcgccgccct gcagaggcag aggcccaccg catccagcag attgctgaag gcgaaaaggt
     1021 gaaacaagtc ctcttggcac aagcagaagc tgagaagatt cgcaaaatcg gggaggcaga
     1081 ggcagcagtc attgaggcca tgggcaaggc cgaggccgag cggatgaagc ttaaagctga
     1141 ggcctaccag aagtacgggg atgcggccaa gatggccctg gtgctggagg ccctgcccca
     1201 gattgctgcc aagatctccg cacccctgac taaagtcgat gagattgtgg ttctcagtgg
     1261 ggacaacagc aaggtgacat cagaagtgaa ccggctgcta gcagaactgc ctgcttctgt
     1321 tcatgccctc actggtgtgg acctctcaaa gataccactg atcaagaatg ccactggtgc
     1381 gcaggtgtga ggctcctggc actcggcctc cagcagctgc ctgcccttcc agcgcctgtt
     1441 ttcaagtcat ggggacagcg agaacattac tgactctggt gccttatttc gtagggacca
     1501 gaagtgctgc gagttcaggc catctctggc tatcttccct tctctcctgt ctgccttctc
     1561 ttttttttcc acaccttcac attaactgcc acatccatct agcctgtctc ctcatctgcc
     1621 atatgtgcct cttcctctgg ctctgttttt ctcccttctc ccaacttcac acacatcatc
     1681 tactttaact gaagttgaac ttgtttatga taatcacttg tctgtctgtc tgtctgtctg
     1741 tctgtctgtc tgtctgtctg tgaggagtgg ccccgctgct tctcaaaatt ctggtgcctt
     1801 caagttctac atgtatctgt ctgtcctcgc tggccctggc agagcccagc atgggtacaa
     1861 gggttctgcg taggacaccc actctcctct ctcctggtct gaatctccaa gcgctaaatg
     1921 ctgttccagg aaacccacat ctcacacagt cttgcccttt gaggcctaag tggccatggc
     1981 ctattggccg tagcataatg cccttcccac accgaggccc gggcctgctg tcatacctgc
     2041 ccctgtcccc tgcaccatag aggcacagaa gcacagcctc ctgtctgtag cagcttcctc
     2101 agtgtactac tgccttagga ggccctgctt gtgctcaggg acatcccttc cgttggcttg
     2161 tccttgattg gtgggtgggt cttaatctga tcccactaag tctttctggg gtcactatct
     2221 agctctgttg gggacaggta aagtttatag gccttcttcc tgctagtgaa tggggctctc
     2281 ccagggggca aggctgtgcc aaccgtaaga gaatcaagtg ctgaagactg gccaaacttc
     2341 actccctcta tgccattttc ttcttggtca gcataatggg tttctagcca tgggaagcag
     2401 ctaagtgttc tgtgtctttg cacctgcacc actggtggca gaagaaccca gtgcccaggt
     2461 gtcctagtaa gggtgcaagg gtgtctgtcc catgggccca gtccccagcc ctggcctgcc
     2521 ccggcctgtg tagctgtgct gcatgtgaat gctgcatgtc tggtctgggg tttggatatt
     2581 gcactgcccc actgcctgtc ccccgctggt gacaataaag atctcttata gccacaaaaa
     2641 aaaaaaaaaa aaa
//
