LOCUS       NM_053430               2184 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus flap structure-specific endonuclease 1 (Fen1),
            transcript variant 2, mRNA.
ACCESSION   NM_053430
VERSION     NM_053430.3
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Szczesny,B., Brunyanszki,A., Olah,G., Mitra,S. and Szabo,C.
  TITLE     Opposing roles of mitochondrial and nuclear PARP1 in the regulation
            of mitochondrial and nuclear DNA integrity: implications for the
            regulation of mitochondrial function
  JOURNAL   Nucleic Acids Res 42 (21), 13161-13173 (2014)
   PUBMED   25378300
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Grolimund,L., Aeby,E., Hamelin,R., Armand,F., Chiappe,D.,
            Moniatte,M. and Lingner,J.
  TITLE     A quantitative telomeric chromatin isolation protocol identifies
            different telomeric states
  JOURNAL   Nat Commun 4, 2848 (2013)
   PUBMED   24270157
REFERENCE   3  (bases 1 to 2184)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   4  (bases 1 to 2184)
  AUTHORS   Saavedra-Rodriguez,L., Vazquez,A., Ortiz-Zuazaga,H.G., Chorna,N.E.,
            Gonzalez,F.A., Andres,L., Rodriguez,K., Ramirez,F., Rodriguez,A.
            and Pena de Ortiz,S.
  TITLE     Identification of flap structure-specific endonuclease 1 as a
            factor involved in long-term memory formation of aversive learning
  JOURNAL   J Neurosci 29 (18), 5726-5737 (2009)
   PUBMED   19420241
  REMARK    GeneRIF: This study defines the fen-1 endonuclease as a new DNA
            recombination/repair factor involved in the formation of long-term
            memories.
REFERENCE   5  (bases 1 to 2184)
  AUTHORS   Dejardin,J. and Kingston,R.E.
  TITLE     Purification of proteins associated with specific genomic Loci
  JOURNAL   Cell 136 (1), 175-186 (2009)
   PUBMED   19135898
REFERENCE   6  (bases 1 to 2184)
  AUTHORS   Kucherlapati,M., Yang,K., Kuraguchi,M., Zhao,J., Lia,M., Heyer,J.,
            Kane,M.F., Fan,K., Russell,R., Brown,A.M., Kneitz,B., Edelmann,W.,
            Kolodner,R.D., Lipkin,M. and Kucherlapati,R.
  TITLE     Haploinsufficiency of Flap endonuclease (Fen1) leads to rapid tumor
            progression
  JOURNAL   Proc Natl Acad Sci U S A 99 (15), 9924-9929 (2002)
   PUBMED   12119409
REFERENCE   7  (bases 1 to 2184)
  AUTHORS   Qiu,J., Bimston,D.N., Partikian,A. and Shen,B.
  TITLE     Arginine residues 47 and 70 of human flap endonuclease-1 are
            involved in DNA substrate interactions and cleavage site
            determination
  JOURNAL   J Biol Chem 277 (27), 24659-24666 (2002)
   PUBMED   11986308
REFERENCE   8  (bases 1 to 2184)
  AUTHORS   Kim,I.S., Lee,M.Y., Lee,I.H., Shin,S.L. and Lee,S.Y.
  TITLE     Gene expression of flap endonuclease-1 during cell proliferation
            and differentiation
  JOURNAL   Biochim Biophys Acta 1496 (2-3), 333-340 (2000)
   PUBMED   10771101
REFERENCE   9  (bases 1 to 2184)
  AUTHORS   Shen,B., Nolan,J.P., Sklar,L.A. and Park,M.S.
  TITLE     Essential amino acids for substrate binding and catalysis of human
            flap endonuclease 1
  JOURNAL   J Biol Chem 271 (16), 9173-9176 (1996)
   PUBMED   8621570
REFERENCE   10 (bases 1 to 2184)
  AUTHORS   Robins,P., Pappin,D.J., Wood,R.D. and Lindahl,T.
  TITLE     Structural and functional homology between mammalian DNase IV and
            the 5'-nuclease domain of Escherichia coli DNA polymerase I
  JOURNAL   J Biol Chem 269 (46), 28535-28538 (1994)
   PUBMED   7961795
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000001.1.
            
            On Nov 25, 2022 this sequence version replaced NM_053430.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF281018.1, SRR26360194.1813609.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-247               JAXUCZ010000001.1  216274150-216274396 c
            248-2184            JAXUCZ010000001.1  216270016-216271952 c
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     gene            1..2184
                     /gene="Fen1"
                     /gene_synonym="FEN-1"
                     /note="flap structure-specific endonuclease 1"
                     /db_xref="GeneID:84490"
                     /db_xref="RGD:621821"
     exon            1..247
                     /gene="Fen1"
                     /gene_synonym="FEN-1"
                     /inference="alignment:Splign:2.1.0"
     exon            248..2184
                     /gene="Fen1"
                     /gene_synonym="FEN-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             267..1409
                     /gene="Fen1"
                     /gene_synonym="FEN-1"
                     /EC_number="3.1.26.4"
                     /codon_start=1
                     /product="flap endonuclease 1"
                     /protein_id="NP_445882.2"
                     /db_xref="GeneID:84490"
                     /db_xref="RGD:621821"
                     /translation="MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLI
                     AVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYIFDGKPPQLKSGELAKRSE
                     RRAEAEKQLQQAQEAGAEEEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAE
                     ASCAALAKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRVLQELGL
                     NQEQFVDLCILLGSDYCESVRGIGPKRAVDLIQKHKSIEEIVRRLDPSKYPVPENWLH
                     KEARQLFLEPEVLDPESVELKWSEPNEEELVKFMCGEKQFSEERIRSGVKRLNKSRQG
                     STQGRLDDFFKVTGSLSSAKRKEPEPKGPAKKKAKTGGAGKFRRGK"
ORIGIN      
        1 gctttggaag gcggcgaagc tggaaacgat ttcggctcga cattgtaaga acaggcttgt
       61 ggctgtccac agaacactgg ggatccaaac cactgctagc taggtgaagc ctcgtgcact
      121 cgaggcggag tgaggtctcg cggaagcgtc tccgagagcg gcgagggccg cgagagcaac
      181 accaagcagc ccggggcttg ggctattctc actgatccga acatctctct tcgctggtag
      241 gaagaagcca ttgctcctgt gttaccatgg gaattcaagg ccttgccaaa ctaattgctg
      301 atgtggcccc cagtgccatc cgtgagaatg acatcaagag ctactttggt cgcaaagtgg
      361 ccattgatgc ctccatgagc atttaccagt tcctgattgc tgttcgtcag ggtggggacg
      421 tactgcagaa cgaggagggg gagaccacca gccacctgat gggcatgttc taccgtacca
      481 tccgcatgat ggagaatggc atcaagccgg tgtatatctt tgatggcaaa ccaccacagc
      541 tgaagtcggg ggagctggcc aaacgcagtg agcggcgtgc agaggccgag aagcagctgc
      601 agcaggctca ggaggcgggg gcagaggagg aggtggaaaa gttcaccaag cggcttgtga
      661 aggtcaccaa gcaacacaac gatgagtgca aacacctgct gagcctcatg ggcatcccat
      721 accttgatgc gcccagcgag gcagaggcca gctgtgctgc cctggcaaag gctggcaaag
      781 tctatgccgc agccacggag gacatggact gcctcacttt tggcagcccc gtgctaatgc
      841 gacacttaac tgccagtgag gccaagaagc tgcccatcca agagttccac ctgagccgcg
      901 tcctgcaaga gctgggtctg aaccaggagc agtttgtgga tctgtgcatc ctgctgggta
      961 gcgactactg tgagagtgtc cgtggcattg gacccaagcg ggctgtggac ctcatccaga
     1021 agcacaagag catcgaggag atcgtgaggc ggctggatcc cagcaagtac cctgttccgg
     1081 aaaactggct ccacaaggaa gcccggcagc tcttcctgga gccggaagta ctggacccag
     1141 agtctgtgga gctgaagtgg agtgagccaa acgaagaaga gttggtcaaa tttatgtgcg
     1201 gtgaaaagca gttttctgag gagcggattc gtagtggggt caaacggctg aataagagcc
     1261 gccaaggcag cacccaggga cgcctagatg atttcttcaa ggtgacaggc tcactctcct
     1321 cagccaagcg caaggagcca gagcccaagg ggcctgccaa gaagaaagca aagactgggg
     1381 gagcaggaaa gttcagaagg ggaaaataaa tgtgtccttc cctccactgt cccttggccc
     1441 caggacagtg ttttgtaccc tcagctagag cacatccctc tcgccctgcg tcttgaggac
     1501 aagttcattg cttcccagcg ctgcccttca gagctttccc tcgcttgacc ctgtgtcagg
     1561 aagcccgtag ctctgctttt cctcattttt agctcaggaa agatgtcagg ctcaaaccac
     1621 ttctcaggtt aatggaccct gtccgttgct ctgtgcaact gctagcagta ttttaaggga
     1681 gaagataagg cagggagaga gtaggaggta atttcccagc tggccagctg gtgggggaca
     1741 ggtgactaga gcgcggctgg cgactgctct ttctaatttc actgtgccct ggaagacagc
     1801 catcagtctg ggattagctg atgagagagc cgcaaagaga aacagaggag tctggcgaca
     1861 acagatttaa tactgactgg ctgttttgtg gacttgctgt ctggtgagcc cagtagttgc
     1921 gcaagggagt gaaaacacag tgtttaggtt taggtttgta gtgtttggtt ttgtgcctcc
     1981 cctctccagt gttgggaatt gaacccaggg caaaggcatt aagtgtacca ctgacctgta
     2041 gctccaagtg atgttctgag gctccaagtg atggcctttc tgaggcagtc aatttaattg
     2101 aggttttgga agaaaaaact tgttcatggg ctgtttctat tttaaaagat gtgaagaaaa
     2161 aaaacaataa aattataaaa gcga
//
