LOCUS       NM_138850               2293 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus fibroblast activation protein, alpha (Fap), mRNA.
ACCESSION   NM_138850
VERSION     NM_138850.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Kilvaer TK, Rakaee M, Hellevik T, Ostman A, Strell C, Bremnes RM,
            Busund LT, Donnem T and Martinez-Zubiaurre I.
  TITLE     Tissue analyses reveal a potential immune-adjuvant function of
            FAP-1 positive fibroblasts in non-small cell lung cancer
  JOURNAL   PLoS One 13 (2), e0192157 (2018)
   PUBMED   29415055
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Tillmanns J, Hoffmann D, Habbaba Y, Schmitto JD, Sedding D,
            Fraccarollo D, Galuppo P and Bauersachs J.
  TITLE     Fibroblast activation protein alpha expression identifies activated
            fibroblasts after myocardial infarction
  JOURNAL   J Mol Cell Cardiol 87, 194-203 (2015)
   PUBMED   26319660
  REMARK    GeneRIF: In this study, we show for the first time the expression
            of FAP in activated fibroblasts after MI and its activation by
            TGFbeta1. Effects of FAP on fibroblast migration and gelatinolytic
            activity indicate a potential role in cardiac wound healing
REFERENCE   3  (bases 1 to 2293)
  AUTHORS   Kilvaer TK, Khanehkenari MR, Hellevik T, Al-Saad S, Paulsen EE,
            Bremnes RM, Busund LT, Donnem T and Martinez IZ.
  TITLE     Cancer Associated Fibroblasts in Stage I-IIIA NSCLC: Prognostic
            Impact and Their Correlations with Tumor Molecular Markers
  JOURNAL   PLoS One 10 (8), e0134965 (2015)
   PUBMED   26252379
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2293)
  AUTHORS   Augoff K, Hryniewicz-Jankowska A, Tabola R, Czapla L, Szelachowski
            P, Wierzbicki J, Grabowski K and Sikorski AF.
  TITLE     Upregulated expression and activation of membrane-associated
            proteases in esophageal squamous cell carcinoma
  JOURNAL   Oncol Rep 31 (6), 2820-2826 (2014)
   PUBMED   24789592
REFERENCE   5  (bases 1 to 2293)
  AUTHORS   Rowton M, Ramos P, Anderson DM, Rhee JM, Cunliffe HE and Rawls A.
  TITLE     Regulation of mesenchymal-to-epithelial transition by PARAXIS
            during somitogenesis
  JOURNAL   Dev Dyn 242 (11), 1332-1344 (2013)
   PUBMED   24038871
REFERENCE   6  (bases 1 to 2293)
  AUTHORS   Ajami K, Abbott CA, Obradovic M, Gysbers V, Kahne T, McCaughan GW
            and Gorrell MD.
  TITLE     Structural requirements for catalysis, expression, and dimerization
            in the CD26/DPIV gene family
  JOURNAL   Biochemistry 42 (3), 694-701 (2003)
   PUBMED   12534281
REFERENCE   7  (bases 1 to 2293)
  AUTHORS   Park JE, Lenter MC, Zimmermann RN, Garin-Chesa P, Old LJ and Rettig
            WJ.
  TITLE     Fibroblast activation protein, a dual specificity serine protease
            expressed in reactive human tumor stromal fibroblasts
  JOURNAL   J Biol Chem 274 (51), 36505-36512 (1999)
   PUBMED   10593948
REFERENCE   8  (bases 1 to 2293)
  AUTHORS   Mueller SC, Ghersi G, Akiyama SK, Sang QX, Howard L,
            Pineiro-Sanchez M, Nakahara H, Yeh Y and Chen WT.
  TITLE     A novel protease-docking function of integrin at invadopodia
  JOURNAL   J Biol Chem 274 (35), 24947-24952 (1999)
   PUBMED   10455171
REFERENCE   9  (bases 1 to 2293)
  AUTHORS   Niedermeyer J, Enenkel B, Park JE, Lenter M, Rettig WJ, Damm K and
            Schnapp A.
  TITLE     Mouse fibroblast-activation protein--conserved Fap gene
            organization and biochemical function as a serine protease
  JOURNAL   Eur J Biochem 254 (3), 650-654 (1998)
   PUBMED   9688278
REFERENCE   10 (bases 1 to 2293)
  AUTHORS   Pineiro-Sanchez ML, Goldstein LA, Dodt J, Howard L, Yeh Y, Tran H,
            Argraves WS and Chen WT.
  TITLE     Identification of the 170-kDa melanoma membrane-bound gelatinase
            (seprase) as a serine integral membrane protease
  JOURNAL   J Biol Chem 272 (12), 7595-7601 (1997)
   PUBMED   9065413
  REMARK    Erratum:[J Biol Chem. 1998 May 22:273(21):13366]
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF493782.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF493782.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5756307,
                                           SAMEA5760383 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q21"
     gene            1..2293
                     /gene="Fap"
                     /note="fibroblast activation protein, alpha"
                     /db_xref="GeneID:192203"
                     /db_xref="RGD:621253"
     exon            1..13
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     CDS             8..2293
                     /gene="Fap"
                     /note="seprase"
                     /codon_start=1
                     /product="prolyl endopeptidase FAP"
                     /protein_id="NP_620205.1"
                     /db_xref="GeneID:192203"
                     /db_xref="RGD:621253"
                     /translation="MKTWLKTVFGVTTLVALALVVICIVLRPSRVYSPEGNTGRSLTL
                     KDILNGTFSYKTYFPNWISEQEYLHQSEDDNIVFYNIETRESYIILSNSTMKSVNATD
                     YGLSPDRQFIYLESDYSKLWRYSYTATYYIYDLQNGEFVRGYELPRPIQYLCWSPVGS
                     KLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDG
                     KYLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPIVRVFIVDTIYPHHVG
                     PIEVPVPEMIASSDYYFTWLTWVTNERVCLQWLKRVQNVSVLSICDFREDWHAWDCPK
                     NQEHIEESRTGWAGGFFVSTPAFSQDAASYYKIFSDKDGYKHIHYIKDTVENAIQITS
                     GKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQY
                     YTASFSYKAKYYALICYGPGLPISTLHDGRTDQEIQVLEENKELENALRNIQLPAVEI
                     KKLEDGGMTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFSVNWITYLASKEG
                     IVVALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEGRIAIWGWS
                     YGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYTERFMGLPTKDDNLEHYKNSTV
                     MARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGR
                     SQNHLYTHMTHFLKQCFSLSD"
     exon            14..98
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            99..197
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            198..292
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            293..367
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            368..420
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            421..493
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            494..614
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            615..769
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            770..873
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1009
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1054
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1055..1159
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1230
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1284
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1409
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1457
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1556
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..1626
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1821
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1822..1876
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..1976
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            1977..2041
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            2042..2114
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            2115..2188
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
     exon            2189..2293
                     /gene="Fap"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tggaaaaatg aagacatggc tgaaaaccgt ctttggagtc actactttgg ttgcgcttgc
       61 tctagtggtg atatgcattg tcttacgtcc ctcaagagtt tacagccctg aaggcaacac
      121 aggaagatct cttaccctga aggatatttt aaatggaaca ttctcctata aaacatattt
      181 tccaaactgg atttcagagc aagaatatct tcatcaatct gaggatgata acatagtctt
      241 ttataatatt gaaacaagag aatcctatat cattttgagt aatagcacca tgaaaagtgt
      301 gaatgctaca gactatggcc tgtcacctga tcggcagttt atatatcttg aaagtgatta
      361 ttcaaagctc tggcgatatt catacacagc gacatactac atctacgacc ttcagaatgg
      421 ggaatttgta agaggatacg agctccctcg tccaattcag tatctgtgct ggtctcccgt
      481 tgggagtaaa ttagcatatg tctatcaaaa caatatttat ttaaaacaaa gaccaggaga
      541 tccacctttt caaataactt acactggaag agaaaataga atatttaatg gaattccaga
      601 ctgggtttat gaagaggaaa tgcttgctac aaaatatgcc ctttggtggt ccccagatgg
      661 aaaatatttg gcatatgtag aatttaatga ttcggacata ccaattattg cctactcgta
      721 ttatggcgat ggacagtatc ctagaactat aaatattcca tacccgaagg ccggagctaa
      781 gaatcccatt gttcgtgtct ttattgttga cactatctac ccacaccatg tgggtcctat
      841 agaagtacca gttccagaga tgatagcctc gagtgactac tatttcacct ggctcacatg
      901 ggtgaccaac gaacgagtat gtttgcagtg gctaaagaga gtgcagaatg tctcagtctt
      961 gtctatatgt gatttcagag aagactggca tgcgtgggat tgtccaaaga accaggagca
     1021 tattgaagaa agcagaacag gatgggctgg tggattcttt gtttccacac cagcttttag
     1081 ccaggatgcc gcttcatact acaaaatatt tagcgacaag gatggttaca aacatattca
     1141 ctacatcaaa gacactgtgg aaaatgctat tcaaattaca agtggcaagt gggaggccat
     1201 atatatattc cgagtaacac aggattcact gttttattct agcaatgaat ttgaaggtta
     1261 ccctggaaga agaaacatct acagaattag cattggaaac tctcctccga gtaagaagtg
     1321 tgttacttgc catctaagga aagaaaggtg ccaatattac acagcaagtt tcagctacaa
     1381 agccaaatac tatgcactca tctgctacgg ccctggcctc ccgatttcca ccctccatga
     1441 tggccgcaca gaccaagaaa tacaagtatt agaagagaac aaagaactgg agaacgctct
     1501 gagaaacatc cagctgcctg cagtggaaat taagaagctt gaagacggtg gaatgacttt
     1561 ctggtacaag atgattcttc ctcctcagtt tgacagatca aagaagtacc ctctgctaat
     1621 tcaagtgtat ggtggtccct gcagccagag tgtaaagtct gtattctctg ttaattggat
     1681 aacttatctt gcaagtaaag aggggatagt cgttgccttg gtagatggcc ggggcactgc
     1741 tttccaaggt gacaaattcc tgcatgccgt gtatcgaaag ctgggtgtgt atgaagttga
     1801 ggaccagctc acagctgtca gaaaattcat agaaatgggt ttcattgatg aaggaagaat
     1861 agccatatgg ggatggtcct acggaggtta tgtttcatcc ctagcccttg cgtccggaac
     1921 tggccttttc aaatgtggta tagcagtggc tccagtctcc agctgggaat actacgcctc
     1981 tatctacacg gagagattca tgggcctccc aacaaaggat gataatcttg aacactataa
     2041 aaattcaact gtgatggcaa gagcagaata tttcagaaat gtagactatc ttctcatcca
     2101 cggaacagca gatgataatg tgcactttca gaactcagca cagattgcta aagctttggt
     2161 taacgcacaa gtggatttcc aggcgatgtg gtactctgac cagaaccatg gcatatcatc
     2221 tgggcgctcc cagaaccact tatacactca catgacgcac ttcctcaagc aatgcttttc
     2281 tttatcagac tga
//
