LOCUS       NM_173137               1442 bp    mRNA    linear   ROD 02-APR-2024
DEFINITION  Rattus norvegicus fatty acid desaturase 3 (Fads3), mRNA.
ACCESSION   NM_173137
VERSION     NM_173137.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Garcia,C., Guillocheau,E., Richard,L., Drouin,G., Catheline,D.,
            Legrand,P. and Rioux,V.
  TITLE     Conversion of dietary trans-vaccenic acid to
            trans11,cis13-conjugated linoleic acid in the rat lactating mammary
            gland by Fatty Acid Desaturase 3-catalyzed methyl-end
            Delta13-desaturation
  JOURNAL   Biochem Biophys Res Commun 505 (2), 385-391 (2018)
   PUBMED   30262139
  REMARK    GeneRIF: physiological evidence demonstrates the existence of in
            vivo synthesis of trans11,cis13-CLA from dietary trans-vaccenic
            acid in rodents; and establishes the FADS3 enzyme as a reliable
            mammalian trans-vaccenate Delta13-desaturase in vivo.
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Blanchard,H., Boulier-Monthean,N., Legrand,P. and Pedrono,F.
  TITLE     The 51 kDa FADS3 is secreted in the ECM of hepatocytes and blood in
            rat
  JOURNAL   J Cell Biochem 115 (1), 199-207 (2014)
   PUBMED   23966218
  REMARK    GeneRIF: FADS3 was secreted specifically by hepatocytes in the
            extracellular matrix where it was located on fibronectin-containing
            fibers.The 51 kDa FADS3 is secreted in the extracellular matrix of
            hepatocytes and blood.
REFERENCE   3  (bases 1 to 1442)
  AUTHORS   Rioux,V., Pedrono,F., Blanchard,H., Duby,C., Boulier-Monthean,N.,
            Bernard,L., Beauchamp,E., Catheline,D. and Legrand,P.
  TITLE     Trans-vaccenate is Delta13-desaturated by FADS3 in rodents
  JOURNAL   J Lipid Res 54 (12), 3438-3452 (2013)
   PUBMED   24070791
  REMARK    GeneRIF: FADS3 is the first 'methyl-end' fatty acid desaturase
            functionally characterized in mammals
REFERENCE   4  (bases 1 to 1442)
  AUTHORS   Pedrono,F., Blanchard,H., Kloareg,M., D'andrea,S., Daval,S.,
            Rioux,V. and Legrand,P.
  TITLE     The fatty acid desaturase 3 gene encodes for different FADS3
            protein isoforms in mammalian tissues
  JOURNAL   J Lipid Res 51 (3), 472-479 (2010)
   PUBMED   19752397
  REMARK    GeneRIF: FADS3 does exist under multiple protein isoforms depending
            on the mammalian tissues.
REFERENCE   5  (bases 1 to 1442)
  AUTHORS   Marquardt,A., Stohr,H., White,K. and Weber,B.H.
  TITLE     cDNA cloning, genomic structure, and chromosomal localization of
            three members of the human fatty acid desaturase family
  JOURNAL   Genomics 66 (2), 175-183 (2000)
   PUBMED   10860662
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AJ494720.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ494720.1, HQ901598.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     gene            1..1442
                     /gene="Fads3"
                     /note="fatty acid desaturase 3"
                     /db_xref="GeneID:286922"
                     /db_xref="RGD:628876"
     exon            1..313
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
     CDS             89..1438
                     /gene="Fads3"
                     /EC_number="1.14.19.-"
                     /note="putative fatty acid desaturase; delta(13)
                     desaturase; delta(13) fatty acid desaturase;
                     trans-vaccenate Delta13-desaturase"
                     /codon_start=1
                     /product="fatty acid desaturase 3"
                     /protein_id="NP_775160.1"
                     /db_xref="GeneID:286922"
                     /db_xref="RGD:628876"
                     /translation="MGGVGEPGGGPGPREGPAPLGAPLPIFRWEQIRQHDLPGDKWLV
                     IERRVYDISRWAQRHPGGSRLIGHHSAEDATDAFHAFHQDLHFVRKFLKPLLIGELAP
                     EEPSQDGAQNAQLIEDFRALRQAAEDMKLFEADTTFFALLLGHILAMELLAWLLVYLL
                     GPGWVSSVLAALILAISQAQCWCLQHDLGHASIFPKSRWNHVAQQFVMGQLKGFSAHW
                     WNFRHFQHHAKPNIFHKDPDVTVAPVFLLGESSVEYGKKKRRYLPYNHQHLYFFLIGP
                     PLLTLVNFEVENLAYMLVCMQWTDLLWAASFYSRFFLSYSPFYGATGTLLLFVAVRVL
                     ESHWFVWITQMNHIPKEIGHEKHRDWASSQLAATCNVEPSLFIDWFSGHLNFQIEHHL
                     FPTMPRHNYRRVAPLVKAFCAKHGLHYEVKPFLTALVDIIGSLKKSGDIWLDAYLHQ"
     exon            314..424
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
     exon            425..622
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
     exon            623..724
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
     exon            725..847
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
     exon            848..908
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
     exon            909..985
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1083
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1180
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1260
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1386
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1442
                     /gene="Fads3"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aggaagaccc ccgattgcga cctcgcctcc ctccatctct cagacctctg ccacgtaccg
       61 gggtccggag gacttgtgca tagcggccat gggcggcgtc ggggagcccg gtgggggacc
      121 cgggccacgg gaggggcccg caccgctggg ggcgcccctg cccatcttcc gctgggagca
      181 gatccgccag catgacctac caggcgacaa gtggctggtc atcgagcgcc gcgtctacga
      241 catcagccgc tgggcacagc ggcacccagg gggtagccgc ctcatcggcc accacagcgc
      301 ggaggacgcc acggatgcct tccacgcctt ccaccaggat ctccattttg tgcgcaagtt
      361 cctgaaaccc ctgttgattg gagagctagc cccagaggaa cccagccagg atggagctca
      421 gaatgcccag ctgatcgagg acttccgagc ccttcgtcag gcagcagagg acatgaagct
      481 gtttgaagct gataccactt tctttgcact cctgctgggc cacatcctgg ctatggagtt
      541 gctggcctgg cttctcgtct acctcttggg ccctggctgg gtatccagtg tccttgctgc
      601 cctcatcctg gccatctctc aggcccagtg ctggtgtctg caacatgatc taggccatgc
      661 ttccatcttc ccaaagtcca ggtggaacca tgtggcccag cagtttgtga tggggcagct
      721 gaaaggcttt tctgcccact ggtggaattt ccgccacttc cagcaccatg ccaaacccaa
      781 tatcttccac aaggatccag atgtgacggt cgcacctgtc ttcctcctgg gggagtcatc
      841 tgtggagtat ggcaagaaga aacgcagata cctgccctac aaccaccagc atctatactt
      901 cttcctgatt ggcccaccgc tgctcacctt ggtgaacttt gaagttgaaa atctggcata
      961 catgctggtg tgcatgcaat ggacggactt gctgtgggct gccagtttct actcccggtt
     1021 cttcttgtcc tactccccct tctatggtgc cactgggaca ctgctcctct tcgttgctgt
     1081 cagggtgctg gagagccact ggttcgtgtg gatcacacag atgaaccaca tccccaagga
     1141 gattggccac gaaaagcatc gggactgggc tagctctcag ctggcagcta cctgcaatgt
     1201 ggaaccttct ctcttcattg actggttcag tgggcacctc aacttccaga ttgaacacca
     1261 cctctttccc acgatgccaa ggcacaacta ccggagggtg gcccccctgg tcaaggcatt
     1321 ctgtgccaag catggtctac actacgaagt gaagcccttc ctcaccgccc tggtggatat
     1381 catcgggtcc ctgaagaagt ctggtgacat ctggctggat gcgtacctcc atcagtgaag
     1441 gc
//
