LOCUS       NP_775160                449 aa            linear   ROD 02-APR-2024
DEFINITION  fatty acid desaturase 3 [Rattus norvegicus].
ACCESSION   NP_775160
VERSION     NP_775160.1
DBSOURCE    REFSEQ: accession NM_173137.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 449)
  AUTHORS   Garcia,C., Guillocheau,E., Richard,L., Drouin,G., Catheline,D.,
            Legrand,P. and Rioux,V.
  TITLE     Conversion of dietary trans-vaccenic acid to
            trans11,cis13-conjugated linoleic acid in the rat lactating mammary
            gland by Fatty Acid Desaturase 3-catalyzed methyl-end
            Delta13-desaturation
  JOURNAL   Biochem Biophys Res Commun 505 (2), 385-391 (2018)
   PUBMED   30262139
  REMARK    GeneRIF: physiological evidence demonstrates the existence of in
            vivo synthesis of trans11,cis13-CLA from dietary trans-vaccenic
            acid in rodents; and establishes the FADS3 enzyme as a reliable
            mammalian trans-vaccenate Delta13-desaturase in vivo.
REFERENCE   2  (residues 1 to 449)
  AUTHORS   Blanchard,H., Boulier-Monthean,N., Legrand,P. and Pedrono,F.
  TITLE     The 51 kDa FADS3 is secreted in the ECM of hepatocytes and blood in
            rat
  JOURNAL   J Cell Biochem 115 (1), 199-207 (2014)
   PUBMED   23966218
  REMARK    GeneRIF: FADS3 was secreted specifically by hepatocytes in the
            extracellular matrix where it was located on fibronectin-containing
            fibers.The 51 kDa FADS3 is secreted in the extracellular matrix of
            hepatocytes and blood.
REFERENCE   3  (residues 1 to 449)
  AUTHORS   Rioux,V., Pedrono,F., Blanchard,H., Duby,C., Boulier-Monthean,N.,
            Bernard,L., Beauchamp,E., Catheline,D. and Legrand,P.
  TITLE     Trans-vaccenate is Delta13-desaturated by FADS3 in rodents
  JOURNAL   J Lipid Res 54 (12), 3438-3452 (2013)
   PUBMED   24070791
  REMARK    GeneRIF: FADS3 is the first 'methyl-end' fatty acid desaturase
            functionally characterized in mammals
REFERENCE   4  (residues 1 to 449)
  AUTHORS   Pedrono,F., Blanchard,H., Kloareg,M., D'andrea,S., Daval,S.,
            Rioux,V. and Legrand,P.
  TITLE     The fatty acid desaturase 3 gene encodes for different FADS3
            protein isoforms in mammalian tissues
  JOURNAL   J Lipid Res 51 (3), 472-479 (2010)
   PUBMED   19752397
  REMARK    GeneRIF: FADS3 does exist under multiple protein isoforms depending
            on the mammalian tissues.
REFERENCE   5  (residues 1 to 449)
  AUTHORS   Marquardt,A., Stohr,H., White,K. and Weber,B.H.
  TITLE     cDNA cloning, genomic structure, and chromosomal localization of
            three members of the human fatty acid desaturase family
  JOURNAL   Genomics 66 (2), 175-183 (2000)
   PUBMED   10860662
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AJ494720.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ494720.1, HQ901598.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMD00132261, SAMD00132262
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Rattus norvegicus"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     Protein         1..449
                     /product="fatty acid desaturase 3"
                     /EC_number="1.14.19.-"
                     /note="putative fatty acid desaturase; delta(13)
                     desaturase; delta(13) fatty acid desaturase;
                     trans-vaccenate Delta13-desaturase"
                     /calculated_mol_wt=51336
     Region          1..21
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1P9.1)"
     Region          27..444
                     /region_name="PLN03199"
                     /note="delta6-acyl-lipid desaturase-like protein;
                     Provisional"
                     /db_xref="CDD:178740"
     Site            138..158
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1P9.1)"
     Site            164..184
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1P9.1)"
     Region          168..417
                     /region_name="Delta6-FADS-like"
                     /note="The Delta6 Fatty Acid Desaturase (Delta6-FADS)-like
                     CD includes the integral-membrane enzymes: delta-4,
                     delta-5, delta-6, delta-8, delta-8-sphingolipid, and
                     delta-11 desaturases found in vertebrates, higher plants,
                     fungi, and bacteria. These desaturases...; cd03506"
                     /db_xref="CDD:239583"
     Site            order(186,190,223,226..227,387,390..391)
                     /site_type="other"
                     /note="putative di-iron ligands [ion binding]"
                     /db_xref="CDD:239583"
     Region          186..190
                     /region_name="Histidine box-1.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5Q0"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1P9.1)"
     Region          223..227
                     /region_name="Histidine box-2.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5Q0"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1P9.1)"
     Site            269..289
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1P9.1)"
     Site            311..331
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1P9.1)"
     Region          387..391
                     /region_name="Histidine box-3.
                     /evidence=ECO:0000250|UniProtKB:Q9Y5Q0"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8K1P9.1)"
     CDS             1..449
                     /gene="Fads3"
                     /coded_by="NM_173137.1:89..1438"
                     /db_xref="GeneID:286922"
                     /db_xref="RGD:628876"
ORIGIN      
        1 mggvgepggg pgpregpapl gaplpifrwe qirqhdlpgd kwlvierrvy disrwaqrhp
       61 ggsrlighhs aedatdafha fhqdlhfvrk flkplligel apeepsqdga qnaqliedfr
      121 alrqaaedmk lfeadttffa lllghilame llawllvyll gpgwvssvla alilaisqaq
      181 cwclqhdlgh asifpksrwn hvaqqfvmgq lkgfsahwwn frhfqhhakp nifhkdpdvt
      241 vapvfllges sveygkkkrr ylpynhqhly ffligppllt lvnfevenla ymlvcmqwtd
      301 llwaasfysr fflsyspfyg atgtlllfva vrvleshwfv witqmnhipk eighekhrdw
      361 assqlaatcn vepslfidwf sghlnfqieh hlfptmprhn yrrvaplvka fcakhglhye
      421 vkpfltalvd iigslkksgd iwldaylhq
//
