LOCUS       NP_112634                444 aa            linear   ROD 02-APR-2024
DEFINITION  acyl-CoA 6-desaturase [Rattus norvegicus].
ACCESSION   NP_112634 XP_346571
VERSION     NP_112634.1
DBSOURCE    REFSEQ: accession NM_031344.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (residues 1 to 444)
  AUTHORS   Reyna Gallegos,S., Torres Arrunategui,G., Valenzuela,R.,
            Rincon-Cervera,M.A. and Villanueva Espinoza,M.E.
  TITLE     Adding a purple corn extract in rats supplemented with chia oil
            decreases gene expression of SREBP-1c and retains Delta5 and Delta6
            hepatic desaturase activity, unmodified the hepatic lipid profile
  JOURNAL   Prostaglandins Leukot Essent Fatty Acids 132, 1-7 (2018)
   PUBMED   29735017
  REMARK    GeneRIF: The effect of dietary plant extracts and vegetable oils on
            hepatic SREBP-1c, Delta5 and Delta6 desaturases has been reported.
REFERENCE   2  (residues 1 to 444)
  AUTHORS   Rioux,V., Choque,B., Ezanno,H., Duby,C., Catheline,D. and
            Legrand,P.
  TITLE     Influence of the cis-9, cis-12 and cis-15 double bond position in
            octadecenoic acid (18:1) isomers on the rat FADS2-catalyzed
            Delta6-desaturation
  JOURNAL   Chem Phys Lipids 187, 10-19 (2015)
   PUBMED   25701799
  REMARK    GeneRIF: We first showed that both recombinant and native rat FADS2
            were able to Delta6-desaturate not only the cis9- but also the
            cis12- and cis15-18:1 isomers.
REFERENCE   3  (residues 1 to 444)
  AUTHORS   Gonzalez,R.S., Rodriguez-Cruz,M., Maldonado,J. and Saavedra,F.J.
  TITLE     Role of maternal tissue in the synthesis of polyunsaturated fatty
            acids in response to a lipid-deficient diet during pregnancy and
            lactation in rats
  JOURNAL   Gene 549 (1), 7-23 (2014)
   PUBMED   25046614
  REMARK    GeneRIF: Results show that genes with the highest expression in the
            mammary gland, liver and adipose tissue were Elovl5, Fads2, and
            Fasn, respectively, in a low-lipid diet than in adequate-lipid
            diet.
REFERENCE   4  (residues 1 to 444)
  AUTHORS   Le,C.H., Mulligan,C.M., Routh,M.A., Bouma,G.J., Frye,M.A.,
            Jeckel,K.M., Sparagna,G.C., Lynch,J.M., Moore,R.L., McCune,S.A.,
            Bristow,M., Zarini,S., Murphy,R.C. and Chicco,A.J.
  TITLE     Delta-6-desaturase links polyunsaturated fatty acid metabolism with
            phospholipid remodeling and disease progression in heart failure
  JOURNAL   Circ Heart Fail 7 (1), 172-183 (2014)
   PUBMED   24284026
  REMARK    GeneRIF: Delta-6-desaturase links polyunsaturated fatty acid
            metabolism with phospholipid remodeling and disease progression in
            heart failure.
            Erratum:[Circ Heart Fail. 2014 Mar 1;7(2):382]
REFERENCE   5  (residues 1 to 444)
  AUTHORS   Hoile,S.P., Irvine,N.A., Kelsall,C.J., Sibbons,C., Feunteun,A.,
            Collister,A., Torrens,C., Calder,P.C., Hanson,M.A., Lillycrop,K.A.
            and Burdge,G.C.
  TITLE     Maternal fat intake in rats alters 20:4n-6 and 22:6n-3 status and
            the epigenetic regulation of Fads2 in offspring liver
  JOURNAL   J Nutr Biochem 24 (7), 1213-1220 (2013)
   PUBMED   23107313
  REMARK    GeneRIF: epigenetic regulation of Fads2 may contribute to short-
            and long-term regulation of PUFA synthesis
REFERENCE   6  (residues 1 to 444)
  AUTHORS   Tang,C., Cho,H.P., Nakamura,M.T. and Clarke,S.D.
  TITLE     Regulation of human delta-6 desaturase gene transcription:
            identification of a functional direct repeat-1 element
  JOURNAL   J Lipid Res 44 (4), 686-695 (2003)
   PUBMED   12562861
  REMARK    GeneRIF: n-6 and n-3 PUFAs suppressed the hepatic expression of
            Fads2 by inhibiting the rate of Fads2 gene transcription. In
            contrast, consumption of the PPARalpha activator WY 14,643
            significantly enhanced the transcription of hepatic DeFads2 by
            500%.
REFERENCE   7  (residues 1 to 444)
  AUTHORS   Guillou,H., Rioux,V., Catheline,D., Thibault,J.N., Bouriel,M.,
            Jan,S., D'Andrea,S. and Legrand,P.
  TITLE     Conversion of hexadecanoic acid to hexadecenoic acid by rat Delta
            6-desaturase
  JOURNAL   J Lipid Res 44 (3), 450-454 (2003)
   PUBMED   12562851
  REMARK    GeneRIF: rat Delta 6-desaturase, which acts on the 18- and
            24-carbon fatty acids of the n-6 and n-3 series, is also able to
            catalyze palmitic acid Delta 6 -desaturation
REFERENCE   8  (residues 1 to 444)
  AUTHORS   D'andrea,S., Guillou,H., Jan,S., Catheline,D., Thibault,J.N.,
            Bouriel,M., Rioux,V. and Legrand,P.
  TITLE     The same rat Delta6-desaturase not only acts on 18- but also on
            24-carbon fatty acids in very-long-chain polyunsaturated fatty acid
            biosynthesis
  JOURNAL   Biochem J 364 (Pt 1), 49-55 (2002)
   PUBMED   11988075
  REMARK    GeneRIF: Delta6-desaturase acts on 24-carbon fatty acids in
            very-long-chain polyunsaturated fatty acid biosynthesis
REFERENCE   9  (residues 1 to 444)
  AUTHORS   Aki,T., Shimada,Y., Inagaki,K., Higashimoto,H., Kawamoto,S.,
            Shigeta,S., Ono,K. and Suzuki,O.
  TITLE     Molecular cloning and functional characterization of rat delta-6
            fatty acid desaturase
  JOURNAL   Biochem Biophys Res Commun 255 (3), 575-579 (1999)
   PUBMED   10049752
REFERENCE   10 (residues 1 to 444)
  AUTHORS   Cho,H.P., Nakamura,M.T. and Clarke,S.D.
  TITLE     Cloning, expression, and nutritional regulation of the mammalian
            Delta-6 desaturase
  JOURNAL   J Biol Chem 274 (1), 471-477 (1999)
   PUBMED   9867867
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC081776.1.
            
            On Dec 17, 2003 this sequence version replaced XP_346571.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC081776.1, FQ210893.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5756307, SAMEA5760400
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Rattus norvegicus"
                     /db_xref="taxon:10116"
                     /chromosome="1"
                     /map="1q43"
     Protein         1..444
                     /product="acyl-CoA 6-desaturase"
                     /EC_number="1.14.19.3"
                     /note="delta-6 fatty acid desaturase; D6D; delta(6)
                     desaturase; fatty acid desaturase 6"
                     /calculated_mol_wt=52250
     Region          20..435
                     /region_name="PLN03198"
                     /note="delta6-acyl-lipid desaturase; Provisional"
                     /db_xref="CDD:178739"
     Site            131..151
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z122.1)"
     Site            158..178
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z122.1)"
     Region          162..412
                     /region_name="Delta6-FADS-like"
                     /note="The Delta6 Fatty Acid Desaturase (Delta6-FADS)-like
                     CD includes the integral-membrane enzymes: delta-4,
                     delta-5, delta-6, delta-8, delta-8-sphingolipid, and
                     delta-11 desaturases found in vertebrates, higher plants,
                     fungi, and bacteria. These desaturases...; cd03506"
                     /db_xref="CDD:239583"
     Site            order(180,184,217,220..221,382,385..386)
                     /site_type="other"
                     /note="putative di-iron ligands [ion binding]"
                     /db_xref="CDD:239583"
     Region          180..184
                     /region_name="Histidine box-1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z122.1)"
     Region          217..221
                     /region_name="Histidine box-2"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z122.1)"
     Site            265..285
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z122.1)"
     Site            306..326
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z122.1)"
     Region          382..386
                     /region_name="Histidine box-3"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9Z122.1)"
     CDS             1..444
                     /gene="Fads2"
                     /gene_synonym="Fadsd6"
                     /coded_by="NM_031344.2:167..1501"
                     /db_xref="GeneID:83512"
                     /db_xref="RGD:68339"
ORIGIN      
        1 mgkggnqgeg stelqapmpt frweeiqkhn lrtdrwlvid rkvynvtkws qrhpgghrvi
       61 ghysgedatd afrafhldld fvgkflkpll igelapeeps ldrgkssqit edfralkkta
      121 edmnlfktnh lffflllshi ivmesiawfi lsyfgngwip tvitafvlat sqaqagwlqh
      181 dyghlsvykk siwnhivhkf vighlkgasa nwwnhrhfqh hakpnifhkd pdikslhvfv
      241 lgewqpleyg kkklkylpyn hqheyfflig ppllipmyfq yqiimtmirr rdwvdlawai
      301 syyarffyty ipfygilgal vflnfirfle shwfvwvtqm nhivmeidld hyrdwfssql
      361 aatcnveqsf fndwfsghln fqiehhlfpt mprhnlhkia plvkslcakh gieyqekpll
      421 ralldivssl kksgelwlda ylhk
//
