LOCUS       NM_183331               8335 bp    mRNA    linear   ROD 31-MAR-2024
DEFINITION  Rattus norvegicus coagulation factor VIII (F8), mRNA.
ACCESSION   NM_183331 XM_217580
VERSION     NM_183331.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Shi,Q., Mattson,J.G., Fahs,S.A., Geurts,A.M., Weiler,H. and
            Montgomery,R.R.
  TITLE     The severe spontaneous bleeding phenotype in a novel hemophilia A
            rat model is rescued by platelet FVIII expression
  JOURNAL   Blood Adv 4 (1), 55-65 (2020)
   PUBMED   31899798
REFERENCE   2  (bases 1 to 8335)
  AUTHORS   Manon-Jensen,T., Karsdal,M.A., Nielsen,L.N., Kjelgaard-Hansen,M.,
            Vandahl,B., Olsen,E.H., Enoksson,M. and Roepstorff,K.
  TITLE     Altered collagen turnover in factor VIII-deficient rats with
            hemophilic arthropathy identifies potential novel serological
            biomarkers in hemophilia
  JOURNAL   J Thromb Haemost 14 (12), 2419-2429 (2016)
   PUBMED   27681457
  REMARK    GeneRIF: analysis of a F8(-/-) rat model of hemophilic arthropathy
            reveals that collagen turnover is altered
REFERENCE   3  (bases 1 to 8335)
  AUTHORS   Sorensen,K.R., Roepstorff,K., Wiinberg,B., Hansen,A.K.,
            Tranholm,M., Nielsen,L.N. and Kjelgaard-Hansen,M.
  TITLE     The F8(-/-) rat as a model of hemophilic arthropathy
  JOURNAL   J Thromb Haemost 14 (6), 1216-1225 (2016)
   PUBMED   27060449
REFERENCE   4  (bases 1 to 8335)
  AUTHORS   Smith,S.A., Travers,R.J. and Morrissey,J.H.
  TITLE     How it all starts: Initiation of the clotting cascade
  JOURNAL   Crit Rev Biochem Mol Biol 50 (4), 326-336 (2015)
   PUBMED   26018600
  REMARK    Review article
REFERENCE   5  (bases 1 to 8335)
  AUTHORS   Nielsen,L.N., Wiinberg,B., Hager,M., Holmberg,H.L., Hansen,J.J.,
            Roepstorff,K. and Tranholm,M.
  TITLE     A novel F8 -/- rat as a translational model of human hemophilia A
  JOURNAL   J Thromb Haemost 12 (8), 1274-1282 (2014)
   PUBMED   24931420
REFERENCE   6  (bases 1 to 8335)
  AUTHORS   Akkarapatumwong,V., Oranwiroon,S., Pung-amritt,P., Treesucon,A.,
            Thanootarakul,P., Veerakul,G., Mahasandana,C., Panyim,S. and
            Yenchitsomanus,P.
  TITLE     Mutations of the factor VIII gene in thai hemophilia A patients
  JOURNAL   Hum Mutat 15 (1), 117-118 (2000)
   PUBMED   10612839
REFERENCE   7  (bases 1 to 8335)
  AUTHORS   VandenDriessche,T., Vanslembrouck,V., Goovaerts,I., Zwinnen,H.,
            Vanderhaeghen,M.L., Collen,D. and Chuah,M.K.
  TITLE     Long-term expression of human coagulation factor VIII and
            correction of hemophilia A after in vivo retroviral gene transfer
            in factor VIII-deficient mice
  JOURNAL   Proc Natl Acad Sci U S A 96 (18), 10379-10384 (1999)
   PUBMED   10468616
REFERENCE   8  (bases 1 to 8335)
  AUTHORS   Nobukata,H., Katsuki,Y., Ishikawa,T., Inokuma,M. and Shibutani,Y.
  TITLE     Effect of dienogest on bleeding time, coagulation, fibrinolysis,
            and platelet aggregation in female rats
  JOURNAL   Toxicol Lett 104 (1-2), 93-101 (1999)
   PUBMED   10048754
REFERENCE   9  (bases 1 to 8335)
  AUTHORS   Knobl,P., Schernthaner,G., Schnack,C., Pietschmann,P., Proidl,S.,
            Prager,R. and Vukovich,T.
  TITLE     Haemostatic abnormalities persist despite glycaemic improvement by
            insulin therapy in lean type 2 diabetic patients
  JOURNAL   Thromb Haemost 71 (6), 692-697 (1994)
   PUBMED   7974333
REFERENCE   10 (bases 1 to 8335)
  AUTHORS   Hellman,L., Smedsrod,B., Sandberg,H. and Pettersson,U.
  TITLE     Secretion of coagulant factor VIII activity and antigen by in vitro
            cultivated rat liver sinusoidal endothelial cells
  JOURNAL   Br J Haematol 73 (3), 348-355 (1989)
   PUBMED   2513867
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from
            JAXUCZ010000018.1.
            
            On Nov 27, 2020 this sequence version replaced NM_183331.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY362193.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-226               JAXUCZ010000018.1  155309-155534
            227-339             JAXUCZ010000018.1  158073-158185
            340-462             JAXUCZ010000018.1  158808-158930
            463-675             JAXUCZ010000018.1  159878-160090
            676-744             JAXUCZ010000018.1  161052-161120
            745-840             JAXUCZ010000018.1  161327-161422
            841-1062            JAXUCZ010000018.1  162033-162254
            1063-1315           JAXUCZ010000018.1  162401-162653
            1316-1487           JAXUCZ010000018.1  163202-163373
            1488-1581           JAXUCZ010000018.1  163490-163583
            1582-1796           JAXUCZ010000018.1  164646-164860
            1797-1947           JAXUCZ010000018.1  166882-167032
            1948-2157           JAXUCZ010000018.1  167895-168104
            2158-5023           JAXUCZ010000018.1  170731-173596
            5024-5177           JAXUCZ010000018.1  176514-176667
            5178-5387           JAXUCZ010000018.1  177193-177402
            5388-5616           JAXUCZ010000018.1  178079-178307
            5617-5799           JAXUCZ010000018.1  178633-178815
            5800-5916           JAXUCZ010000018.1  180044-180160
            5917-5988           JAXUCZ010000018.1  180394-180465
            5989-6074           JAXUCZ010000018.1  180901-180986
            6075-6230           JAXUCZ010000018.1  181745-181900
            6231-6375           JAXUCZ010000018.1  182499-182643
            6376-6524           JAXUCZ010000018.1  182736-182884
            6525-6701           JAXUCZ010000018.1  183169-183345
            6702-8335           JAXUCZ010000018.1  185050-186683
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="18"
                     /map="18p13"
     gene            1..8335
                     /gene="F8"
                     /note="coagulation factor VIII"
                     /db_xref="GeneID:302470"
                     /db_xref="RGD:727845"
     exon            1..226
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..6857
                     /gene="F8"
                     /note="coagulation factor VIII, procoagulant component"
                     /codon_start=1
                     /product="coagulation factor VIII precursor"
                     /protein_id="NP_899160.2"
                     /db_xref="GeneID:302470"
                     /db_xref="RGD:727845"
                     /translation="MRAARGLCFFLSLCALCSCATRKYYLGAVELPWDYVSGGASGAQ
                     RSDSRFPPGTPAPSAHVVHTKTVFVEYMDRFFHTAKPRPLWMGLLGPTIWTEVHDTVV
                     ITLKNMASHPVSLHAVGMSFWKASEGAAYDDHSSPAEKDDDKVLPGESHTYAWQVLGE
                     SGPMASDPPCLTYAYLSHVDLVRDLNSGLVGALLVCKEGRLSADRTRMPPHFVLLFAV
                     FDEGRSWHAATRDPASTEAQPTVHTVNGYTNRTLPGLTGCRGTSVYWHVMAVGTTPDI
                     HSILLEGHTFSVRSHRQASLEISPMTLLTAQTWLMDLGRFLLFCHISSHRHGGMEAYV
                     QVDNCPEAPQGQGKRDEDTEDYDDLDSEMDVFTWDPDAAPFVQVRSAAKRHPKTWVHY
                     IAAEEGDWDYAPAAATLEDGSLRNRYLGRGPGRIGSKYKKVRFVAYTDGTFHTREATP
                     REAGLLGPLLYGEVGDSLLIVFKNRASRAYNIHPHGIRDVGAVHAGRLPRGVKHVKDL
                     PIRPGETFKYRWTLTAEDGPARSDARCVTRYYASAVDPERDLASGLIGPLLICCKESV
                     DQRGNQMMSDERNVILFSVFDENRSWYISENMRRFLPDEAHVQLQDPEFEASNLMHSI
                     NGYVFDSLELRVCLHEVAAWHVLSVGAQTDFLSVFFSGHTFRHRAVYADTLTVFPHSG
                     VTVFMSMDNPGVWVLGCHNPDFRESGMTALLKVSSCDEGVSDYYGETYEGVLAPLAND
                     NAVDPRSFSQNSNHLHSRKKKSNTPMRFPLSHPTQHGSFWSDSQGDVHDAVHRAHSPA
                     AAHSNEGPAEAAQLEPKPHPGEKMALPPSWLRAKRSLVTTMEAKRKKLDLQVQVSGLP
                     DDRTTAVVAPDDPMAACKKAGSSGFPDESSPAALGKKMYPRIRPHGPLSIIEGNRDSN
                     SSDSTLMYRLGSPPGDATSWTENRRGLGEKRSHRVAFLARGNTLLSDVDEKSHAPGPT
                     SVGNSTAAVQDTILEICSEILEVTPPIHDRILSDKSATYLRPHRTPDRITSTERKDIR
                     HKKDGDLVPQDADDTSAPFSEAPFLSESTDWLKEANGDNSAKPEQEPSPEQLVYLMYI
                     KCMENQSFSSEKNKMIAGQGGFTKNTGLEDTVFPRKTSVFLTTVAKRQESGRHNQENI
                     PQAVTEKEAPIEEKGALPQVHIATGSKNFPRDMFVLGTGQNINLHEETYVPVHKNVPW
                     TRNPTDTRQIPMVHFFKKRKEEETNSGGLVNKTRETVRNYPSQKNSVARRREQASGRI
                     KASARWLPDVNRSIQSLLKQIDHRKERKKFIIESRSADSSGTKSPTQTNHSPSHVVKM
                     SAFPPRDIRRIESRDSSQVWVSSYAYDFETGSSRIRESSPFLNETETDNPSLAVPPRE
                     RFVRRGRFASPEKVNTHSAPCDKPENVVSSEPVSPGEAVTVALPPHVSTQEEEPLPAG
                     SSCERRGHVDLFLEISLQRTRGPVERSRGRRSGGDTEGHTENPGKTPSPLPQMPKDQR
                     GSEAGYPKICRWRDGTVLPPRPHNLSLGAKKKPNLPRREATRVEQGEAPSLVSPEPLV
                     LRRRPREASTLLPGGEMREDDGDGVTAEYDDDDDVMAEYDDDAVTVDTPEDFDIYGEE
                     AGQGPRGFQQKTRHYFIAAVEELWDYGVRVSPGALGDRAWSGDAARFRKVVFREFTDG
                     SFTQRVHRGELDAHLGLLGPYIRAEVEDNIVVTFRNQASRPYSFYSSLVSYPEADGGA
                     APRSNFVRPNETKTYFWRVRPHMAPTDGEFDCKAWAYFSDVDLERDLHSGLIGPLLVC
                     HASTLHPSHARPLAVQEFALLFAVFDETKSWYCAENLDRKCRPPRGTPAGDPAWREEY
                     RFHAVNGYVADALLGLVMAEGRRTRWHLLSMGDPGHAQSVHFSAHSVTVRDGGEHRTA
                     VCNLYPGVFTTVEMLPSRAGIWRVESLVGEHLQAGMSALFLVYSTRCQVPLGMASGYI
                     RDSQITASGYHGLWTPQLARLHNAGSVNAWSAKEPFAWIKVDLLAPMILHGIETQGAR
                     HRLSSLYVSQFIIMYSLDGKQWLSYRGNSTGSLMVFFGNVDASTVRHNRFDPPIVARY
                     IRVHPTHASIRTALRMELLGCDLNSCSAPLGMESGWISDTQVTASSHLSTTFSAWPPA
                     LARLHLRGGANAWRPQVNDPTQWLQVDLQRTVKVTGVVTQGARSLLTAMFVKKFLVST
                     SQDGRHWTHVLQDGKVKVFQGNRDASTPMVNSLHPPRFTRYLRIHPQVWERQIALRLE
                     ILGCEAQQLD"
     exon            227..339
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            340..462
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            463..675
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            676..744
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            745..840
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            841..1062
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1315
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1487
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            1488..1581
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1796
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            1797..1947
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            1948..2157
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..5023
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            5024..5177
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            5178..5387
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            5388..5616
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            5617..5799
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            5800..5916
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            5917..5988
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            5989..6074
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            6075..6230
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            6231..6375
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            6376..6524
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            6525..6701
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
     exon            6702..8335
                     /gene="F8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgctcggga atttttaaag ttatatctgg gccgggggtt ctgtgggtgg cgctcgcgcc
       61 tctctccggt ggcgggagac atgcgcgcgg cgcgcggcct gtgcttcttc ctcagcctct
      121 gcgcgctctg ctcctgcgcc acccggaagt actacctggg cgccgtggag ttgccatggg
      181 actatgtgag cggtggtgct tccggtgccc agcgcagcga ctccaggttc cctcccggga
      241 caccagcgcc gtctgcgcat gttgtgcaca cgaagacggt gtttgtggag tacatggacc
      301 ggtttttcca cacggcaaag cccaggccac tgtggatggg tctcctaggc cccaccattt
      361 ggaccgaggt tcacgacact gtggtcatca cactcaaaaa catggcttct catcccgtca
      421 gtctccacgc tgtcggtatg tccttctgga aggcgtccga gggcgcggca tacgacgacc
      481 acagcagccc ggccgagaag gacgacgaca aagtcctccc cggcgagagc cacacttacg
      541 cgtggcaggt cctgggggag agcggcccaa tggcttccga ccccccgtgc ctcacctatg
      601 cctacctgtc tcatgtggac ctcgtgaggg acctgaactc gggcctcgtc ggggccctgc
      661 tcgtgtgcaa agaaggccgt ctgtccgcag acagaacacg gatgccaccc cacttcgtgc
      721 tgctttttgc tgtcttcgat gaagggcgga gctggcacgc agcaacacgt gaccccgcgt
      781 ccaccgaggc ccagcccacg gtgcacactg tgaatggcta cacgaaccgg acactgccgg
      841 gtctgaccgg atgccgcgga acatccgtgt actggcacgt gatggcggtg ggcaccacgc
      901 ccgacataca ctccatcctc ctcgaaggcc acacattctc tgtgaggagc caccgtcagg
      961 cttccctgga gatctcacct atgactctgc tcacggctca gacgtggctg atggacctgg
     1021 ggcggttcct gctcttctgt cacatctcct cccacagaca cggtggcatg gaagcctacg
     1081 tccaggtgga caactgcccc gaggcgcccc aggggcaggg gaaaagggac gaggacacag
     1141 aggactatga tgatctggac tcggaaatgg acgtcttcac ctgggacccc gatgccgccc
     1201 ccttcgtcca ggtccgctca gcagccaaga gacaccccaa gacctgggtg cactacatcg
     1261 cagcagagga gggggactgg gactacgcgc ctgcggccgc caccctagaa gacgggagcc
     1321 tgcggaaccg gtacctgggg cgcggccctg gccgcatcgg cagcaaatat aagaaagtca
     1381 ggttcgtggc ctacacggac ggcacctttc acacgcgtga ggccaccccg cgtgaagctg
     1441 ggctcctagg gccactgctc tacggggagg tcggagactc actgttgatt gtgttcaaga
     1501 accgagcgag ccgggcatac aacatccacc ctcatgggat ccgtgacgtt ggcgctgtgc
     1561 atgcagggag gctgccacga ggggtgaagc acgtgaagga tttgccgatc cgcccaggag
     1621 agacgttcaa gtacaggtgg acgctgaccg cagaggatgg gcctgcaagg tcggatgcgc
     1681 ggtgtgtgac gcgctactat gcgagcgctg tggatcccga gagagacctg gcttccgggc
     1741 tgattggccc cctcctcatc tgctgtaaag aatctgtaga tcaaaggggg aaccagatga
     1801 tgtcagacga gaggaacgtc atcctgttct ccgtgtttga tgagaaccgg agctggtaca
     1861 tctcggagaa catgcgacgc ttcctccccg acgaggcaca tgtgcagctg caggaccccg
     1921 agttcgaggc ctccaacctc atgcacagca ttaatggcta cgtcttcgac agcctggagc
     1981 tgcgcgtttg cctgcacgag gtggctgcct ggcacgtgct cagcgtcggc gcgcagactg
     2041 acttcctgtc tgtcttcttc tctggacaca cgttcagaca cagggcagtg tacgcagaca
     2101 cactcacggt gttcccgcac tcgggggtca ccgtcttcat gtccatggat aacccaggtg
     2161 tgtgggtgct ggggtgccac aacccggact tccgggagag cgggatgacc gcactgctga
     2221 aggtttccag ttgtgacgag ggcgtgagtg attactacgg agagacctat gagggcgttc
     2281 tggccccgtt ggccaatgac aacgccgtgg accccaggag cttctcccag aattcaaatc
     2341 atctgcacag caggaaaaag aaaagcaaca cgccgatgcg cttcccactg agccatccta
     2401 cccagcatgg ctccttttgg tcggacagcc agggagatgt ccatgacgct gttcatagag
     2461 cccattcacc cgctgcagca cacagcaatg aaggcccagc cgaagcagcc cagctcgagc
     2521 ccaaacccca tcccggtgag aaaatggcac ttcctccttc ttggttaaga gcaaagagaa
     2581 gtctggtgac aaccatggaa gccaagcgga agaaactgga tctccaggtc caggtttctg
     2641 gtttgcccga cgacagaaca accgcagtgg ttgcgccgga cgatccgatg gcggcttgta
     2701 agaaggcggg ttcttcaggg tttccagatg aatcgagtcc tgctgcattg ggtaagaaaa
     2761 tgtatccccg tatcaggccc cacggacctt taagcatcat tgaaggaaac agggattcca
     2821 actcgtcgga ctcgacttta atgtataggc tgggaagtcc accaggagac gccacatcat
     2881 ggacggagaa ccgcagagga cttggcgaga agaggtctca cagggtcgct ttcctggcca
     2941 gaggtaacac tttactcagc gacgttgatg aaaaatcgca tgcccccggc ccaacatcag
     3001 ttgggaacag cacagcagct gtgcaggaca ccatattaga gatctgcagt gagattctcg
     3061 aggtcacacc tccgattcat gacaggatac tttcagacaa aagtgctaca tatttgagac
     3121 cgcaccgtac gcccgacaga atcacctcga cggaaaggaa agacatacgt cacaaaaaag
     3181 atggagatct tgttccgcag gatgcagatg acacaagcgc accattctcg gaggcgcctt
     3241 tcctgtcaga atccacagat tggttaaaag aggccaatgg ggataactcc gcaaaacctg
     3301 agcaagaacc cagtccagag caattagtgt atttaatgta tataaagtgt atggaaaatc
     3361 agagtttctc gtcagagaag aataaaatga tagcagggca aggtggattt accaagaaca
     3421 caggacttga agacacggtt tttcccagaa agacgagtgt gtttctcacc actgtggcta
     3481 agcggcaaga aagtgggagg cacaaccaag aaaacattcc tcaggcagtg acagagaagg
     3541 aagcgccaat cgaagagaag ggagctctgc cccaggtgca catagcgact ggctctaaga
     3601 acttcccgag agacatgttt gtactcggta ctgggcaaaa tataaatctc cacgaagaga
     3661 catatgtacc agttcacaaa aacgtcccat ggacaagaaa cccaacagac acaagacaga
     3721 ttcccatggt tcatttcttt aagaaaagga aggaggagga aaccaattca ggaggcttgg
     3781 taaataaaac gagagaaacg gtgagaaact acccaagcca gaagaatagc gttgctcgac
     3841 gtagggaaca ggcttcggga cgaatcaaag catccgctcg ttggcttcca gacgtaaacc
     3901 gttcaatcca gagtctcctt aaacagattg accaccgcaa ggaaaggaaa aagttcatta
     3961 tagaatctcg ctccgcagat tcttctggga ctaaaagtcc cactcagacc aatcattctc
     4021 cctcacacgt tgtgaaaatg tcagcattcc caccgagaga catccgaagg atcgagtcgc
     4081 gagacagttc tcaggtctgg gtgtcgtcct atgcttacga ctttgagaca ggaagttcta
     4141 gaattcgaga gagcagtcct ttcctgaatg aaactgaaac ggataaccct tctctcgccg
     4201 tgccaccgcg ggagaggttc gtgcgtcgag gaaggttcgc ctccccagaa aaagtgaaca
     4261 cacactcagc gccgtgtgac aaacccgaaa atgttgtctc ctcggaacca gtgtcgcctg
     4321 gcgaagctgt cacagtcgca ctccctcctc acgtctccac tcaggaggag gaacctttgc
     4381 ctgcaggaag tagctgtgag cgtcgcggac atgtggatct ctttctcgag atctctctcc
     4441 agagaacacg ggggcccgtt gaacggagta gaggaaggag gtctggggga gacacggaag
     4501 gtcacacaga gaaccccgga aagactccct ccccactgcc acagatgccg aaagatcagc
     4561 gtgggtctga agcggggtac ccgaaaatct gccgctggag agacggcact gttttgcccc
     4621 cgaggcctca caacctttcc ctaggagcaa agaaaaaacc aaatctgccc cgaagagaag
     4681 ccactcgggt ggagcaaggc gaggctccga gcctggtctc tccagagccc ctggtgctga
     4741 gacgacgtcc aagggaagct agcacccttc tgccaggagg agagatgcga gaggacgacg
     4801 gtgatggtgt cacggcggag tacgacgacg acgatgacgt catggcagag tatgacgacg
     4861 atgctgtcac ggtggacacg cccgaggatt tcgacatcta tggcgaggag gcagggcagg
     4921 gaccccgcgg ctttcagcag aaaacgcggc actatttcat cgcggccgtg gaggagcttt
     4981 gggactacgg ggtgcgtgtg tcgcctggag ctctgggaga cagggcgtgg agtggggacg
     5041 cagctcggtt ccggaaagtg gttttccgtg agttcacgga cgggtcgttc acgcagcgtg
     5101 tgcaccgtgg ggagctggac gcacacctgg ggctattggg gccatacata cgggcggaag
     5161 tggaggacaa catcgtggtg accttccgga accaggcctc ccgcccctac tccttctact
     5221 ccagcctcgt gtcctacccg gaagccgacg gtggagctgc accaaggagc aactttgtgc
     5281 ggcccaacga gaccaaaact tacttctgga gagtccggcc ccacatggcg cccacggacg
     5341 gcgagtttga ctgcaaggcc tgggcctatt tctccgacgt ggacctcgag cgagacctgc
     5401 actcggggct gattgggcca ctgctcgtgt gccacgcctc caccctgcac ccctcgcacg
     5461 cgcggccgct cgcagtgcag gagttcgcgc tgctcttcgc ggtgttcgat gagaccaaga
     5521 gctggtactg cgctgagaac ctggaccgga agtgcaggcc gccccgtggc acccccgcgg
     5581 gagaccccgc ctggagagaa gagtaccgct tccatgccgt gaatggttac gtcgcagatg
     5641 ctctgcttgg cctggtgatg gcggaaggtc ggaggacgag gtggcatctg ctcagcatgg
     5701 gcgaccccgg gcacgcacag tccgtgcatt tcagcgcgca cagcgtcacc gtgcgggacg
     5761 ggggcgagca caggacggcg gtgtgcaacc tgtacccagg cgtgttcaca accgtggaga
     5821 tgttgccgtc cagagcgggg atctggcggg tggagagcct ggtgggcgag cacctacagg
     5881 ctgggatgag cgccctcttc ctggtgtaca gcacgcgctg ccaggtcccg cttggcatgg
     5941 cttccggtta catccgggac tcgcagatca cggcttccgg ctaccacggg ctgtggaccc
     6001 cgcagctcgc gaggcttcac aacgcgggat ccgtgaatgc ctggagtgcc aaggagccat
     6061 tcgcgtggat caaggtggat ctcctggcgc ccatgattct tcacggcatc gagacgcagg
     6121 gcgcccgaca cagactttcc agcctttatg tgtctcagtt catcatcatg tacagcctgg
     6181 atgggaagca gtggctgagc taccgaggaa actccacggg aagcctgatg gttttcttcg
     6241 gcaacgtgga cgcgtccact gtgaggcaca accgcttcga ccccccgatc gttgcccgct
     6301 acatccgtgt gcaccccaca catgccagca tccgtaccgc cctgcgcatg gagctgctcg
     6361 gctgcgacct taacagctgc agcgcgcccc tgggcatgga gagtggatgg atctcggaca
     6421 cgcaggtcac tgcctcgtcc cacctcagca ccacgttctc tgcttggccg cctgcgctcg
     6481 ctcggcttca cctccggggt ggggccaacg cctggcgacc ccaggtgaat gatcccacac
     6541 agtggctgca ggtggacttg cagaggactg tgaaagtcac cggcgtcgtc acccagggtg
     6601 cgcggtctct cctcactgcc atgtttgtga aaaagttcct cgtctccacc agccaagatg
     6661 gccgccactg gactcacgtc ttgcaagacg gcaaggtcaa ggtttttcag ggtaaccggg
     6721 atgcgtccac gcccatggtg aactctctgc accccccgcg cttcactcga taccttcgga
     6781 ttcaccccca agtgtgggag cggcaaattg ctctgcggct cgagatcctg gggtgtgagg
     6841 ctcagcagct ggactgaggt tgccttcgca cctgcgcctg tcacccctcc tcagttcgag
     6901 ggttgtgtca ctgctgtaaa gggaagcatg gcagcgcata agcagatgtg gcgctggagg
     6961 agtgaccgcg attccacatc tggacccacg ggcagcagga ggacagagac gcgccaccac
     7021 cattttattt aagaccatac atgatgtata accatatatg atcacacacg accccactct
     7081 gatactctcc aaattattca tcctgaattt cctgaagact taactttcga cgttcggagt
     7141 ggaagccagg aagcgatgca ctaggtttta cacgttcggt gtatatggtg tatatatgat
     7201 gtatattgtg cacggccaaa accaaaaaaa aaaaatccgt gatagaatga gaactggaaa
     7261 aaatgattct ttttattcct tctcatccat gaacttcatt cactcattca tggccccact
     7321 atatgtagcc ttccatgttc tcttttcttc atgttatact gtatctaaat gtatgtttgt
     7381 ttacatatat aatatataaa actatataac tatacatagg tatatataag tatatataag
     7441 tatataacta tgtaactata atgctatata tataactata agtatatata actacataac
     7501 tatatacata actatatata taaactctat ataactgtat ataactaagt atatataact
     7561 atatataagt atataaaact atatataact atatgtaaat atgtatcatc agctagatta
     7621 tatatatgag agaaaacata tagatctttt ctttctgaca ttgcgtgacc tcatttaata
     7681 acatacattc taaggctatt tatgttcctg aaagttttat cttcatttaa actttaattg
     7741 aaaacccgaa acactgaaac ttttaggaaa aaaagaaaaa gaaacccaca ggatatatat
     7801 atgtatatat atatatatat atgtatatat atatacatat atatatatat atattttcac
     7861 ccttgaatat atacataccc ttgaagatct cagtctaggc aggaactttc aaatggagca
     7921 taaactcgac aggaactgac cccaagtatc aacatgtgaa aaacatgcgc agcaagagaa
     7981 acgaccatca gttcacacta cgtccagagg ataagagaca aatctttacc agctacactt
     8041 cacaaaaggg ctaattaata tgcaggggct ggagggatgg ctcagtggtt agagcaccgg
     8101 ccgttctccc agaggtccct agttcaattc ccagtgactg cacggtggct cgcaaccctc
     8161 tgtcatggga tccgtgtcca cgcacatgga gcagtctctg ccctgtcacg cacacagagt
     8221 gctactatct tcaataaatg aataattctt aaaaagggct gatatccagg atttacgaga
     8281 actgcggaaa ttaaacacca atgagattaa attgttaagc cacaaatggg caaat
//
