LOCUS       NM_001399506            2887 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus exostosin glycosyltransferase 2 (Ext2),
            transcript variant 1, mRNA.
ACCESSION   NM_001399506
VERSION     NM_001399506.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Shevelev,O.B., Rykova,V.I., Fedoseeva,L.A., Leberfarb,E.Y.,
            Dymshits,G.M. and Kolosova,N.G.
  TITLE     Expression of Ext1, Ext2, and heparanase genes in brain of
            senescence-accelerated OXYS rats in early ontogenesis and during
            development of neurodegenerative changes
  JOURNAL   Biochemistry (Mosc) 77 (1), 56-61 (2012)
   PUBMED   22339633
  REMARK    GeneRIF: Ext2 gene is involved in heparan sulfate proteoglycans
            metabolism.
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   3  (bases 1 to 2887)
  AUTHORS   Gonzalez-Begne,M., Lu,B., Han,X., Hagen,F.K., Hand,A.R.,
            Melvin,J.E. and Yates,J.R.
  TITLE     Proteomic analysis of human parotid gland exosomes by
            multidimensional protein identification technology (MudPIT)
  JOURNAL   J Proteome Res 8 (3), 1304-1314 (2009)
   PUBMED   19199708
REFERENCE   4  (bases 1 to 2887)
  AUTHORS   Presto,J., Thuveson,M., Carlsson,P., Busse,M., Wilen,M.,
            Eriksson,I., Kusche-Gullberg,M. and Kjellen,L.
  TITLE     Heparan sulfate biosynthesis enzymes EXT1 and EXT2 affect NDST1
            expression and heparan sulfate sulfation
  JOURNAL   Proc Natl Acad Sci U S A 105 (12), 4751-4756 (2008)
   PUBMED   18337501
REFERENCE   5  (bases 1 to 2887)
  AUTHORS   Busse,M., Feta,A., Presto,J., Wilen,M., Gronning,M., Kjellen,L. and
            Kusche-Gullberg,M.
  TITLE     Contribution of EXT1, EXT2, and EXTL3 to heparan sulfate chain
            elongation
  JOURNAL   J Biol Chem 282 (45), 32802-32810 (2007)
   PUBMED   17761672
REFERENCE   6  (bases 1 to 2887)
  AUTHORS   Stickens,D., Zak,B.M., Rougier,N., Esko,J.D. and Werb,Z.
  TITLE     Mice deficient in Ext2 lack heparan sulfate and develop exostoses
  JOURNAL   Development 132 (22), 5055-5068 (2005)
   PUBMED   16236767
REFERENCE   7  (bases 1 to 2887)
  AUTHORS   Busse,M. and Kusche-Gullberg,M.
  TITLE     In vitro polymerization of heparan sulfate backbone by the EXT
            proteins
  JOURNAL   J Biol Chem 278 (42), 41333-41337 (2003)
   PUBMED   12907669
REFERENCE   8  (bases 1 to 2887)
  AUTHORS   McCormick,C., Duncan,G., Goutsos,K.T. and Tufaro,F.
  TITLE     The putative tumor suppressors EXT1 and EXT2 form a stable complex
            that accumulates in the Golgi apparatus and catalyzes the synthesis
            of heparan sulfate
  JOURNAL   Proc Natl Acad Sci U S A 97 (2), 668-673 (2000)
   PUBMED   10639137
REFERENCE   9  (bases 1 to 2887)
  AUTHORS   Philippe,C., Porter,D.E., Emerton,M.E., Wells,D.E., Simpson,A.H.
            and Monaco,A.P.
  TITLE     Mutation screening of the EXT1 and EXT2 genes in patients with
            hereditary multiple exostoses
  JOURNAL   Am J Hum Genet 61 (3), 520-528 (1997)
   PUBMED   9326317
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000003.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC166748.1, SRR26643291.9520.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760476,
                                           SAMEA5760494 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-183               JAXUCZ010000003.1  100253242-100253424 c
            184-749             JAXUCZ010000003.1  100243800-100244365 c
            750-839             JAXUCZ010000003.1  100242224-100242313 c
            840-956             JAXUCZ010000003.1  100235011-100235127 c
            957-1152            JAXUCZ010000003.1  100226037-100226232 c
            1153-1292           JAXUCZ010000003.1  100223791-100223930 c
            1293-1386           JAXUCZ010000003.1  100218662-100218755 c
            1387-1518           JAXUCZ010000003.1  100186957-100187088 c
            1519-1708           JAXUCZ010000003.1  100164335-100164524 c
            1709-1875           JAXUCZ010000003.1  100156323-100156489 c
            1876-2019           JAXUCZ010000003.1  100132978-100133121 c
            2020-2148           JAXUCZ010000003.1  100129661-100129789 c
            2149-2231           JAXUCZ010000003.1  100128327-100128409 c
            2232-2887           JAXUCZ010000003.1  100120776-100121431 c
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="3"
                     /map="3q31"
     gene            1..2887
                     /gene="Ext2"
                     /note="exostosin glycosyltransferase 2"
                     /db_xref="GeneID:311215"
                     /db_xref="RGD:1307707"
     exon            1..183
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            184..749
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     CDS             214..2370
                     /gene="Ext2"
                     /EC_number="2.4.1.224"
                     /note="isoform 1 is encoded by transcript variant 1;
                     exostoses (multiple) 2; exostosin 2"
                     /codon_start=1
                     /product="exostosin-2 isoform 1"
                     /protein_id="NP_001386435.1"
                     /db_xref="GeneID:311215"
                     /db_xref="RGD:1307707"
                     /translation="MCASVKSNIRGPALIPRMKTKHRIYYVTLFSIVLLGLIATGMFQ
                     FWPHSIESSSDGGVEKRSIREVPVVRLTADSPIPERGDLSCRMHTCFDVYRCGFNPKN
                     KIKVYIYPLKKYVDDAGVPVSSAISREYNELLTAISDSDYYTDDISRACLFVPSIDVL
                     NQNPLRIKETAQALAQLPRWDRGTNHLLFNMLPGGPPDYNTALDVPRDRALLAGGGFS
                     TWTYRQGYDVSIPVFSPLSAEVALPEKAPGSRRYFLLSSQMAIHPEYREELEALQAKH
                     RESVLVLDKCTNLSEGVLSIRKRCHQHQVFDYPQVLQEATFCTVLRGARLGQAVLSDV
                     LQAGCVPVVIADSYILPFSEVLDWKRASVVIPEEKMSDVYSILQNIPQRQIEEMQRQA
                     RWFWEAYFRSIKAIALATLQIINDRIYPYAAISYEEWNDPPAVKWASVSNPLFLPLIP
                     PQSQGFTAIVLTYDRVESLFRVITEVSKVPSLSKLLVVWNNQNKNPPEESLWPKIRVP
                     LKVVRTAENKLSNRFFPYDEIETEAVLAIDDDIIMLTSDELQFGYEVWREFPDRLVGY
                     PGRLHLWDHEMNKWKYESEWTNEVSMVLTGAAFYHKYFNYLYTYKMPGDIKNWVDAHM
                     NCEDIAMNFLVANVTGKAVIKVTPRKKFKCPECTAIDGLSLDQTHMVERSECINKFAS
                     VFGTMPLKVVEHRADPVLYKDDFPEKLKSFPNIGSL"
     exon            750..839
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            840..956
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1152
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1292
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            1293..1386
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            1387..1518
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1708
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            1709..1875
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            1876..2019
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            2020..2148
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            2149..2231
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2887
                     /gene="Ext2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtgcgcgcc gtgtcagacg tggggaactg gaggccggcg cagcgttccc cggctggctc
       61 tggcttacct ggtgctggcg gcggcctccc gtcaccgcgc gatccgacac ggccgtggct
      121 cgcgccccgc gcccaaagcc ggtggcggag cgcggggccg agcgggaggc ggccggttcc
      181 gaggtgtgtg aggacgagga catctgtgtc attatgtgtg cgtccgtcaa gtccaacatc
      241 cggggtcctg ccctcatccc aaggatgaag accaagcacc ggatctacta cgtcaccctg
      301 ttctccatcg tcctcctggg cctcatcgcc actggcatgt ttcagttctg gcctcactcc
      361 atcgagtcct ccagcgatgg gggtgtggag aaacgcagca tccgggaagt gccagtggtc
      421 aggctgacag ctgacagccc tatcccagag cggggggatc tcagctgtag aatgcacacg
      481 tgtttcgatg tctaccgctg cggcttcaac ccaaagaaca aaatcaaggt gtacatctac
      541 cccctaaaga agtacgtgga tgacgccggt gttccggtga gcagcgccat ctcccgggag
      601 tacaatgaac tgctcacagc catctcagac agtgactact acacagatga catcagccga
      661 gcctgtctgt tcgtcccctc cattgatgtg ctgaaccaga acccccttcg aatcaaggag
      721 acagcgcaag ccctggccca gcttcccagg tgggatcgag ggacaaatca cctgctgttc
      781 aacatgttgc ctggaggtcc tccagattac aacactgccc tggatgtccc cagagacagg
      841 gcactgctgg ctggtggcgg cttttctacc tggacttacc ggcagggcta cgatgtcagc
      901 attcctgttt tcagcccact gtcagccgaa gtggctctcc cagagaaagc accaggttcc
      961 cggcgatact tcctgctgtc ctctcagatg gccatccacc ctgagtacag agaggaacta
     1021 gaagccctcc aggccaaaca cagagagtcc gtgttagtcc tagacaaatg caccaatctc
     1081 tcagagggcg tcctttccat ccgaaagcgc tgccaccagc accaggtctt tgattacccc
     1141 caagtactgc aggaggctac tttttgtacg gttcttcgag gagctcggct gggccaggca
     1201 gtgctgagcg atgtcttaca ggctggctgt gtcccagttg tcattgcaga ctcttacatt
     1261 ctgcctttct ctgaagttct ggactggaag agggcatctg tggtcattcc agaggagaag
     1321 atgtcagatg tgtacagcat cctgcagaac atcccacaga ggcagattga agagatgcag
     1381 agacaggcac gatggttctg ggaggcatac tttcggtcca ttaaagccat cgccctggcc
     1441 accctacaga tcatcaatga caggatctat ccatatgcag ccatttccta tgaagagtgg
     1501 aatgaccctc ctgctgtgaa gtgggctagt gtgagcaacc cgctcttcct cccactgatc
     1561 ccaccacagt cgcaaggctt cactgccatt gtcctcacct atgaccgtgt ggagagcctc
     1621 ttccgggtca tcaccgaggt gtccaaggtg cccagtctgt ccaagctgct ggtggtctgg
     1681 aataatcaga ataaaaaccc tccagaagaa tctctgtggc ccaaaatccg tgttcccctg
     1741 aaggttgtga ggactgcaga aaacaagcta agtaatcgtt tcttccctta cgatgaaatc
     1801 gagacagagg ccgtgctggc catcgatgat gatatcatca tgctgacctc tgatgagctg
     1861 cagtttggtt atgaggtctg gcgggaattt cctgaccggc tggtgggcta ccctggccgc
     1921 ctgcatctct gggaccacga gatgaacaag tggaagtatg agtctgagtg gaccaatgag
     1981 gtatccatgg tgctcacggg tgcagccttc tatcacaagt attttaatta cctgtatacc
     2041 tacaaaatgc ctggggacat caagaactgg gtagatgctc acatgaactg tgaagatatt
     2101 gccatgaatt tcctagtggc caatgtcaca gggaaagctg tcatcaaggt aaccccaaga
     2161 aagaagttca agtgtcctga gtgcacagcc atcgacggac tctcactaga ccagactcac
     2221 atggtagaga ggtctgagtg catcaataag tttgcttccg tcttcggaac gatgcccctc
     2281 aaggtggtgg agcaccgagc agaccctgta ctctacaaag atgacttccc tgagaaactg
     2341 aagagtttcc ccaacatcgg cagcttgtga gctcaccggt tcccagggct gtggaggagc
     2401 cctacaacat tcacggtggg ggtgaggagg ccagcctggc tcagacttcc ccaaacacac
     2461 tggagcctct tgagctatga gccctgcttt gtcctctcat ctccgtggac tctgaaaatg
     2521 ccaaaggaag ggagcctcag ggacaagctg cagcttggag cccagcctat cgctcttggg
     2581 ttgccaaccc agctcaccat ctcactgacc ctgcagctgc ctcagagggt cagctgtcca
     2641 gctccagatc ccaggagtca cagcagaggc atgcgattgt ttcacatctg cagactctgg
     2701 ggaaatggca aacctcaaac gtgctacaga tcccagtacc tctgagggtt agaactgtgc
     2761 ttgggacccg agtcatgagc ccaagtacac agcagagcac acgctgccaa gatgtgtcct
     2821 gacagtccct gaacaagtct ttgttttggc tttttgatat ggttaaaatt attttttact
     2881 ccttttt
//
