LOCUS       NM_001130540            3474 bp    mRNA    linear   ROD 26-FEB-2024
DEFINITION  Rattus norvegicus exostosin glycosyltransferase 1 (Ext1), mRNA.
ACCESSION   NM_001130540
VERSION     NM_001130540.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Zhuang,L., Gerber,S.D., Kuchen,S., Villiger,P.M. and Trueb,B.
  TITLE     Deletion of exon 8 from the EXT1 gene causes multiple
            osteochondromas (MO) in a family with three affected members
  JOURNAL   Springerplus 5, 71 (2016)
   PUBMED   26839764
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Liu,H., Wu,S., Duan,L., Zhu,W., Zhang,S., Hu,X., Jia,W., Yang,G.,
            Liu,C., Li,W., Yang,L., Guo,L., Lin,Y., Wang,Y., He,M., Yang,Z.,
            He,Y., Cai,Z. and Wang,D.
  TITLE     Identification of a novel EXT1 mutation in patients with hereditary
            multiple exostosis by exome sequencing
  JOURNAL   Oncol Rep 33 (2), 547-552 (2015)
   PUBMED   25421355
REFERENCE   3  (bases 1 to 3474)
  AUTHORS   Cao,L., Liu,F., Kong,M., Fang,Y., Gu,H., Chen,Y., Zhao,C., Zhang,S.
            and Bi,Q.
  TITLE     Novel EXT1 mutation identified in a pedigree with hereditary
            multiple exostoses
  JOURNAL   Oncol Rep 31 (2), 713-718 (2014)
   PUBMED   24297320
REFERENCE   4  (bases 1 to 3474)
  AUTHORS   Shevelev,O.B., Rykova,V.I., Fedoseeva,L.A., Leberfarb,E.Y.,
            Dymshits,G.M. and Kolosova,N.G.
  TITLE     Expression of Ext1, Ext2, and heparanase genes in brain of
            senescence-accelerated OXYS rats in early ontogenesis and during
            development of neurodegenerative changes
  JOURNAL   Biochemistry (Mosc) 77 (1), 56-61 (2012)
   PUBMED   22339633
  REMARK    GeneRIF: Ext1 gene is involved in heparan sulfate proteoglycans
            metabolism.
REFERENCE   5  (bases 1 to 3474)
  AUTHORS   Ogata-Iwao,M., Inatani,M., Iwao,K., Takihara,Y.,
            Nakaishi-Fukuchi,Y., Irie,F., Sato,S., Furukawa,T., Yamaguchi,Y.
            and Tanihara,H.
  TITLE     Heparan sulfate regulates intraretinal axon pathfinding by retinal
            ganglion cells
  JOURNAL   Invest Ophthalmol Vis Sci 52 (9), 6671-6679 (2011)
   PUBMED   21743013
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3474)
  AUTHORS   Lin,X., Wei,G., Shi,Z., Dryer,L., Esko,J.D., Wells,D.E. and
            Matzuk,M.M.
  TITLE     Disruption of gastrulation and heparan sulfate biosynthesis in
            EXT1-deficient mice
  JOURNAL   Dev Biol 224 (2), 299-311 (2000)
   PUBMED   10926768
REFERENCE   7  (bases 1 to 3474)
  AUTHORS   McCormick,C., Duncan,G., Goutsos,K.T. and Tufaro,F.
  TITLE     The putative tumor suppressors EXT1 and EXT2 form a stable complex
            that accumulates in the Golgi apparatus and catalyzes the synthesis
            of heparan sulfate
  JOURNAL   Proc Natl Acad Sci U S A 97 (2), 668-673 (2000)
   PUBMED   10639137
REFERENCE   8  (bases 1 to 3474)
  AUTHORS   McCormick,C., Leduc,Y., Martindale,D., Mattison,K., Esford,L.E.,
            Dyer,A.P. and Tufaro,F.
  TITLE     The putative tumour suppressor EXT1 alters the expression of
            cell-surface heparan sulfate
  JOURNAL   Nat Genet 19 (2), 158-161 (1998)
   PUBMED   9620772
REFERENCE   9  (bases 1 to 3474)
  AUTHORS   Hecht,J.T., Hogue,D., Wang,Y., Blanton,S.H., Wagner,M.,
            Strong,L.C., Raskind,W., Hansen,M.F. and Wells,D.
  TITLE     Hereditary multiple exostoses (EXT): mutational studies of familial
            EXT1 cases and EXT-associated malignancies
  JOURNAL   Am J Hum Genet 60 (1), 80-86 (1997)
   PUBMED   8981950
REFERENCE   10 (bases 1 to 3474)
  AUTHORS   Ahn,J., Ludecke,H.J., Lindow,S., Horton,W.A., Lee,B., Wagner,M.J.,
            Horsthemke,B. and Wells,D.E.
  TITLE     Cloning of the putative tumour suppressor gene for hereditary
            multiple exostoses (EXT1)
  JOURNAL   Nat Genet 11 (2), 137-143 (1995)
   PUBMED   7550340
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            FM064038.1, BE126756.1, BC105839.1, FM083888.1 and DV719494.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC105839.1, SRR8487231.1114.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMD00132261,
                                           SAMD00132262 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-367               FM064038.1         1-367
            368-685             BE126756.1         88-405
            686-2492            BC105839.1         1-1807
            2493-2926           FM083888.1         117-550
            2927-3474           DV719494.1         17-564              c
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10116"
                     /chromosome="7"
                     /map="7q31"
     gene            1..3474
                     /gene="Ext1"
                     /note="exostosin glycosyltransferase 1"
                     /db_xref="GeneID:299907"
                     /db_xref="RGD:1587375"
     exon            1..1082
                     /gene="Ext1"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..2361
                     /gene="Ext1"
                     /EC_number="2.4.1.225"
                     /note="exostoses (multiple) 1"
                     /codon_start=1
                     /product="exostosin-1"
                     /protein_id="NP_001124012.1"
                     /db_xref="GeneID:299907"
                     /db_xref="RGD:1587375"
                     /translation="MQAKKRYFILLSAGSCLALLFYFGGVQFRASRSHSRREEHSGRN
                     GLHQPSPDHFWPRFPDALRPFFPWDQLENEDSSVHISPRQKRDANSSIYKGKKCRMES
                     CFDFTLCKKNGFKVYVYPQQKGEKIAESYQNILAAIEGSRFYTSDPSQACLFVLSLDT
                     LDRDQLSPQYVHNLRSKVQSLHLWNNGRNHLIFNLYSGTWPDYTEDVGFDIGQAMLAK
                     ASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVFKGKRYLTGIG
                     SDTRNALYHVHNGEDVLLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFC
                     LVPRGRRLGSFRFLEALQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPST
                     IRSIHQDKILALRQQTQFLWEAYFSSVEKIVLTTLEIIQDRIFKHISRNSLIWNKHPG
                     GLFVLPQYSSYLGDFPYYYANLGLKPPSKFTAVIHAVTPLVSQSQPVLKLLVAAAKSQ
                     YCAQIIVLWNCDKPLPAKHRWPATAVPVIVIEGESKVMSSRFLPYDNIITDAVLSLDE
                     DTVLSTTEVDFAFTVWQSFPERIVGYPARSHFWDNSKERWGYTSKWTNDYSMVLTGAA
                     IYHKYYHYLYSHYLPASLKNMVDQLANCEDILMNFLVSAVTKLPPIKVTQKKQYKETM
                     MGQTSRASRWADPDHFAQRQSCMNTFASWFGYMPLIHSQMRLDPVLFKDQVSILRKKY
                     RDIERL"
     exon            1083..1176
                     /gene="Ext1"
                     /inference="alignment:Splign:2.1.0"
     exon            1177..1284
                     /gene="Ext1"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1404
                     /gene="Ext1"
                     /inference="alignment:Splign:2.1.0"
     exon            1405..1537
                     /gene="Ext1"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1656
                     /gene="Ext1"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1752
                     /gene="Ext1"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1842
                     /gene="Ext1"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..2003
                     /gene="Ext1"
                     /inference="alignment:Splign:2.1.0"
     exon            2004..2175
                     /gene="Ext1"
                     /inference="alignment:Splign:2.1.0"
     exon            2176..3474
                     /gene="Ext1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagcggagag tttgaagtct ttacaggcgg gaagatggcg gactggagct gaaagtgttg
       61 attgggaaac ttgggtgatt cttgtgttta tttacaatcc tcttgaccca ggcaggacac
      121 atgcaggcca aaaaacgcta tttcatcctg ctctcagctg gctcttgtct cgcccttttg
      181 ttttattttg gaggcgtgca gtttagggca tcgaggagcc acagccggag agaagagcac
      241 agtggtcgga atggcttgca ccaacccagt ccggatcatt tctggccccg cttcccggac
      301 gctctgcgcc ccttctttcc ttgggatcaa ttggaaaacg aggattccag cgtgcacatt
      361 tccccccggc agaagcggga cgccaactcg agcatctaca aaggcaagaa gtgccgcatg
      421 gagtcctgct tcgatttcac cctttgcaag aaaaacggct tcaaagtcta cgtctacccg
      481 cagcagaaag gggagaaaat tgccgaaagt taccaaaaca ttctagcggc catcgagggc
      541 tccaggttct acacctcgga ccccagccag gcgtgcctct ttgtcctgag tctggatact
      601 ttagacagag accagttatc acctcagtat gtgcacaatt tgagatccaa agtgcagagt
      661 ctccacttgt ggaacaatgg taggaatcat ttaattttta atttatattc tggcacttgg
      721 cctgactaca ccgaggacgt ggggtttgac atcggccagg cgatgctggc caaagccagc
      781 atcagtactg aaaacttccg accaaacttt gatgtttcta ttcccctctt ttctaaggat
      841 catcccagga caggagggga gagggggttt ttgaaattta acaccatccc tcccctcagg
      901 aagtacatgc tggtattcaa ggggaagagg tacctgacag ggatagggtc agacaccagg
      961 aatgccttat atcacgtcca taacggggag gacgtcttgc tcctcaccac ctgcaagcat
     1021 ggcaaagact ggcaaaagca caaggattct cgctgtgaca gagacaacac cgagtatgag
     1081 aaatatgatt atcgggaaat gctgcacaat gccactttct gtctggttcc tcgtggtcgc
     1141 aggcttgggt ccttcagatt cctggaggct ttgcaggctg cctgtgtccc tgtaatgctc
     1201 agcaatggat gggagttgcc attctccgaa gtgataaatt ggaaccaagc tgccgtcata
     1261 ggcgatgaga gattgctatt acagattcct tctacaatca ggtctattca tcaggataaa
     1321 atcctagcac ttagacagca gacacagttc ttgtgggagg cttatttttc ttcagttgag
     1381 aagattgtat taactacact agagattatt caggacagaa tattcaagca catatcacgt
     1441 aacagtttaa tatggaacaa acatcctgga ggattgttcg tcctaccaca gtattcatcc
     1501 tacctgggag attttcctta ctactatgct aatttaggtc taaagccccc ctccaaattc
     1561 actgcagtca tccatgctgt gacccccctg gtctctcagt cccagccagt gttgaagctt
     1621 cttgtggcag cagccaaatc ccagtactgt gcacagatta tagttctgtg gaattgtgac
     1681 aagcctctac cagccaaaca ccgctggcct gccactgccg tgcctgtcat cgtcattgaa
     1741 ggagaaagca aggttatgag cagccggttt ctgccctatg acaacatcat cactgatgct
     1801 gtgctcagcc ttgatgagga cactgtgctt tcaaccacgg aagtggattt tgccttcact
     1861 gtatggcaga gcttcccgga gaggattgtg ggatatcctg cacgcagcca tttctgggat
     1921 aactcaaagg agcgatgggg atatacatcc aagtggacga atgactactc catggtgttg
     1981 acaggagctg ctatctacca caaatattat cactacctgt attcccatta cctgccagcc
     2041 agcctgaaga acatggtaga ccaactggcc aactgtgagg acattctcat gaatttcctg
     2101 gtgtctgctg tgacgaaatt gcctccaatc aaagtgaccc agaagaaaca gtataaggag
     2161 acaatgatgg gacagacttc ccgagcatct cgctgggctg accctgacca ctttgcccag
     2221 cgacagagct gcatgaatac gtttgccagc tggtttggct acatgccgct gatccactct
     2281 cagatgaggc tcgacccggt cctctttaaa gaccaagtct caatcctgag gaagaaatac
     2341 agagacattg aacgactttg aggaatccca ctgagtgggg gaggggaggc aagatgggac
     2401 tggagtccag ttgctctctc ctcccagtgc agatctgctc atgcccatcg gtggagccag
     2461 actgtgccaa gtatcaaaaa atgaaaaaaa aataaatata aaaaaaacaa aaaagatttt
     2521 aaaaaagaaa aggaaaaaaa cagcttagat gaacacagtg acaaaactcg gctggaatcc
     2581 tggctcctgg ggccgcgcca gactgctcca ttcacctcac tggcttctgt gtcccacgac
     2641 taggttgtgt acagtttaat tatggaacat taaataatta tttttgaaat gattgctatg
     2701 caggtttaaa cttttttaat gatcaaaact attaaaaacc agagttcttt ctttaatcaa
     2761 aattgtgttg gttgtgaata tttctaagct gatgctcccc tccctacaga cctcgttgcc
     2821 atgggtcacc caggatttcc tgcaatgaca gaaacagaca tcatgggaaa cacaatcagt
     2881 tatttgacat agtgtcagca aagcactggg catacactaa aacaagttca tagtccagtt
     2941 tagatgtttg gattcccctc agtctctaaa cttgctgcat gagaaaaggt gttgactcca
     3001 ggtaggcagt tctaactgaa aaattttaat ataaatatgg taatcttttt acatggggat
     3061 agggaggggg gagatagacc aagcaaacaa gcaaacacta gtagatttaa gaaatgttga
     3121 acaagaaacc aaacaatccc cagcatgtta gtgatggaaa tcccacaact gaaacccaat
     3181 acgtcctaat ggttggtcaa taggctcaac caccaaccca tatatctttt gggggaaccc
     3241 tatgctttag aagccaaagg gggaaaaatg ggtccagatt atttataaga caagtggttt
     3301 aatgtgtttg gaaaaactca agttgcaaag gtttctctgt tgaaaaagaa gcgattatgt
     3361 gtaactccac accgtttaac aagaggatga ctgtgaaaaa ggtacctaac tgggggcatg
     3421 tcttgtcaag gaacttattg ggataatcat tgatttaagc aaaaaaaaaa aaaa
//
