LOCUS       NM_019622               3322 bp    mRNA    linear   ROD 30-MAR-2024
DEFINITION  Rattus norvegicus espin (Espn), mRNA.
ACCESSION   NM_019622
VERSION     NM_019622.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ahmed,Z.M., Jaworek,T.J., Sarangdhar,G.N., Zheng,L., Gul,K.,
            Khan,S.N., Friedman,T.B., Sisk,R.A., Bartles,J.R., Riazuddin,S. and
            Riazuddin,S.
  TITLE     Inframe deletion of human ESPN is associated with deafness,
            vestibulopathy and vision impairment
  JOURNAL   J Med Genet 55 (7), 479-488 (2018)
   PUBMED   29572253
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Lelli,A., Michel,V., Boutet de Monvel,J., Cortese,M.,
            Bosch-Grau,M., Aghaie,A., Perfettini,I., Dupont,T., Avan,P.,
            El-Amraoui,A. and Petit,C.
  TITLE     Class III myosins shape the auditory hair bundles by limiting
            microvilli and stereocilia growth
  JOURNAL   J Cell Biol 212 (2), 231-244 (2016)
   PUBMED   26754646
REFERENCE   3  (bases 1 to 3322)
  AUTHORS   Merritt,R.C., Manor,U., Salles,F.T., Grati,M., Dose,A.C.,
            Unrath,W.C., Quintero,O.A., Yengo,C.M. and Kachar,B.
  TITLE     Myosin IIIB uses an actin-binding motif in its espin-1 cargo to
            reach the tips of actin protrusions
  JOURNAL   Curr Biol 22 (4), 320-325 (2012)
   PUBMED   22264607
REFERENCE   4  (bases 1 to 3322)
  AUTHORS   Revenu,C., Ubelmann,F., Hurbain,I., El-Marjou,F., Dingli,F.,
            Loew,D., Delacour,D., Gilet,J., Brot-Laroche,E., Rivero,F.,
            Louvard,D. and Robine,S.
  TITLE     A new role for the architecture of microvillar actin bundles in
            apical retention of membrane proteins
  JOURNAL   Mol Biol Cell 23 (2), 324-336 (2012)
   PUBMED   22114352
REFERENCE   5  (bases 1 to 3322)
  AUTHORS   Schwander,M., Kachar,B. and Muller,U.
  TITLE     Review series: The cell biology of hearing
  JOURNAL   J Cell Biol 190 (1), 9-20 (2010)
   PUBMED   20624897
  REMARK    Review article
REFERENCE   6  (bases 1 to 3322)
  AUTHORS   Sekerkova,G., Loomis,P.A., Changyaleket,B., Zheng,L., Eytan,R.,
            Chen,B., Mugnaini,E. and Bartles,J.R.
  TITLE     Novel espin actin-bundling proteins are localized to Purkinje cell
            dendritic spines and bind the Src homology 3 adapter protein
            insulin receptor substrate p53
  JOURNAL   J Neurosci 23 (4), 1310-1319 (2003)
   PUBMED   12598619
REFERENCE   7  (bases 1 to 3322)
  AUTHORS   Zheng,L., Sekerkova,G., Vranich,K., Tilney,L.G., Mugnaini,E. and
            Bartles,J.R.
  TITLE     The deaf jerker mouse has a mutation in the gene encoding the espin
            actin-bundling proteins of hair cell stereocilia and lacks espins
  JOURNAL   Cell 102 (3), 377-385 (2000)
   PUBMED   10975527
REFERENCE   8  (bases 1 to 3322)
  AUTHORS   Chen,B., Li,A., Wang,D., Wang,M., Zheng,L. and Bartles,J.R.
  TITLE     Espin contains an additional actin-binding site in its N terminus
            and is a major actin-bundling protein of the Sertoli cell-spermatid
            ectoplasmic specialization junctional plaque
  JOURNAL   Mol Biol Cell 10 (12), 4327-4339 (1999)
   PUBMED   10588661
REFERENCE   9  (bases 1 to 3322)
  AUTHORS   Bartles,J.R., Zheng,L., Li,A., Wierda,A. and Chen,B.
  TITLE     Small espin: a third actin-bundling protein and potential forked
            protein ortholog in brush border microvilli
  JOURNAL   J Cell Biol 143 (1), 107-119 (1998)
   PUBMED   9763424
REFERENCE   10 (bases 1 to 3322)
  AUTHORS   Bartles,J.R., Wierda,A. and Zheng,L.
  TITLE     Identification and characterization of espin, an actin-binding
            protein localized to the F-actin-rich junctional plaques of Sertoli
            cell ectoplasmic specializations
  JOURNAL   J Cell Sci 109 (Pt 6), 1229-1239 (1996)
   PUBMED   8799813
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000005.1.
            
            On Feb 5, 2015 this sequence version replaced NM_019622.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U46007.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760389 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-309               JAXUCZ010000005.1  167942676-167942984 c
            310-503             JAXUCZ010000005.1  167939468-167939661 c
            504-690             JAXUCZ010000005.1  167927923-167928109 c
            691-873             JAXUCZ010000005.1  167927624-167927806 c
            874-1005            JAXUCZ010000005.1  167927317-167927448 c
            1006-1207           JAXUCZ010000005.1  167924337-167924538 c
            1208-1464           JAXUCZ010000005.1  167922869-167923125 c
            1465-1692           JAXUCZ010000005.1  167919836-167920063 c
            1693-1885           JAXUCZ010000005.1  167919553-167919745 c
            1886-2031           JAXUCZ010000005.1  167917189-167917334 c
            2032-2295           JAXUCZ010000005.1  167916850-167917113 c
            2296-2375           JAXUCZ010000005.1  167912328-167912407 c
            2376-2387           JAXUCZ010000005.1  167912229-167912240 c
            2388-3322           JAXUCZ010000005.1  167909271-167910205 c
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q36"
     gene            1..3322
                     /gene="Espn"
                     /gene_synonym="Je"
                     /note="espin"
                     /db_xref="GeneID:56227"
                     /db_xref="RGD:620652"
     exon            1..309
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     CDS             16..2532
                     /gene="Espn"
                     /gene_synonym="Je"
                     /note="Jerker, deafness locus; actin cytoskeletal
                     regulatory protein; ectoplasmic specialization protein"
                     /codon_start=1
                     /product="espin"
                     /protein_id="NP_062568.2"
                     /db_xref="GeneID:56227"
                     /db_xref="RGD:620652"
                     /translation="MALEQAMQAARRGDLDVLRSLHAAGLLGPSLRDPLDALPVHHAA
                     RSGKLHCLRYLVEEVALPAVSRARNGATPAHDAAATGYLSCLQWLLTQGGCRVQEKDN
                     SGATVLHLAARFGHPDVVNWLLYQGGANSAITTDTGALPIHYAAAKGDLPSMKLLVGH
                     YPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQECSADPHLRAQDGMTPLHAAAQMGH
                     NPVLVWLVSFADVSFSEQDHDGATAMHFAASRGHTKVLSWLLLHGAEISQDLWGGTPL
                     HDAAENGELECCQILAVNGAGLDVRDHDGYTAADLADFNGHTHCSRYLRTVQTLSLEH
                     RVLSRDPSMDLEAKQPDSGMSSPNTTMSVQPPNFDLGSPTSTLSNYDSCSSSHSSSKG
                     QRSTRGARSSDLQSYMDMLNPEPRSKQGKPSSLPPPPPPSFPPPPPPGTQLPPPPPGY
                     PAPNPPVGLHLDNIYMQTKNKLRHVEVDSLKKEPSSGDGYSGLRRQDSGLLRQDSELL
                     LRHNTGLRRQDSDRKQRSFSKQPSTGDYYRQLGRSPGEPLAARPGMAHSEEAALLPGN
                     HVHNGCSADSKASRELPPPPPPPPLPEALSSPPPAPPLPIEGAGAACGQRRSSSSTGS
                     TKSFNMMSPTGDNSELLAEIKAGKSLKPTPQSKGLTTVFSGSGQPASQPESPQPAVSP
                     GPSRARSPTPPASGPQPLLNGSIVPAPPATLAPGVHLDVEALIPTLDEQGRPIPEWKR
                     QVMVRKLQQKMQEEEEQRRKEEEEEARLASLPAWRRDILRKKLEEEREQKRKEEERQK
                     LEEIQRAKEQSEKLRTLGYDEAKLAPWQRQVILKKGEIPK"
     exon            310..503
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            504..690
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            691..873
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1005
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            1006..1207
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1464
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1692
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1885
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..2031
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2295
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            2296..2375
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2387
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
     exon            2388..3322
                     /gene="Espn"
                     /gene_synonym="Je"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gccgagggtg acaccatggc cctggaacag gcgatgcagg cggcacggcg gggcgacctg
       61 gacgtgctga ggtccctgca cgccgccggc ctgctggggc cttctctgcg cgacccgcta
      121 gacgccctgc cggtgcacca tgcggcccgc tcaggcaagc tgcactgttt gcgctacttg
      181 gtggaggagg ttgccctccc agctgtgtcc cgcgcgcgca acggcgccac accagcccat
      241 gatgccgctg ccacgggcta cctctcttgc ctgcagtggc tgctcacaca gggtggctgc
      301 agggtgcagg aaaaagataa ctctggtgcc acggtcctgc acctggctgc ccgctttggc
      361 cacccggacg tggtgaactg gctgctgtac cagggcggtg cgaactctgc catcaccaca
      421 gacacgggcg ccctgcctat ccactatgct gccgccaaag gagatctccc ctccatgaag
      481 cttcttgtcg ggcactaccc tgagggagtg aatgcccaaa ccaacaacgg tgccacgccc
      541 ctgtacctgg cgtgccagga gggccacctg gaagtgacga agtaccttgt gcaggagtgc
      601 agtgcagatc cgcacctgcg cgcccaagac ggcatgaccc ccctgcatgc cgcggcgcag
      661 atgggccaca acccagtcct ggtgtggctg gtgagctttg cggacgtgag cttttcggag
      721 caggaccacg acggcgccac agccatgcac tttgcagcca gccgcggcca caccaaagtg
      781 ctcagctggc tcctgctgca cggcgcagag atctcccagg acctgtgggg cgggaccccg
      841 ctgcatgatg ctgctgagaa cggggaactg gagtgctgcc agatcctcgc ggtgaatggt
      901 gcggggctgg acgtccgcga ccacgatggg tatacggctg cggacctggc tgatttcaat
      961 ggccacaccc actgctcccg ctacctacgt acggtgcaaa ccctgagctt ggaacaccga
     1021 gtcctgtccc gggatccatc catggacctg gaggcaaagc agccggactc aggcatgtct
     1081 tcacccaaca ccaccatgtc ggtccagccg ccgaactttg accttggctc acccaccagc
     1141 accctctcca actatgactc ctgctcctcc agccattcca gcagcaaggg tcagcgctct
     1201 actcgaggtg ccagatcctc agacttacag agctacatgg acatgctgaa cccagagcct
     1261 cggagcaagc aagggaagcc ttcatctcta ccaccaccgc caccaccaag cttccctcca
     1321 ccaccacccc caggcacaca gctgccccca cctccaccag gctacccagc tcccaatccc
     1381 cctgtggggc tgcatttgga taacatttac atgcagacca agaacaaact ccgccacgtg
     1441 gaggtggact ccctcaagaa ggagccgagc tccggcgacg gctactcggg gctacgcagg
     1501 caggattccg ggctgctcag gcaggattcg gagctgctgc tcaggcacaa caccggactg
     1561 cgcaggcagg actccgaccg caaacagcgt tcattcagta aacagcccag cacgggggac
     1621 tactaccgcc agctgggccg cagcccgggg gagccgctgg ccgcacgccc gggcatggcc
     1681 cacagcgagg aggcggcgct gctccccggg aaccacgtgc acaacggctg ctccgcggac
     1741 tccaaagcgt ccagggagct gccgccgcca ccgccgccgc cgcccctgcc cgaggccctg
     1801 agttcgccgc cacccgcccc acctctgccc atcgagggcg cgggcgcagc ctgcggacag
     1861 cggcgttcct catcttctac tggcagcacc aaatctttca acatgatgtc cccaacgggt
     1921 gacaactcgg agttgctggc tgaaataaaa gcaggcaaga gtctgaaacc aacaccgcag
     1981 agcaaggggc tgacaacagt gttctcaggc agcgggcagc cagcctccca gcctgagtca
     2041 ccacagcctg cggtgtcacc tgggccatct cgggcccgga gccccacccc accagcctct
     2101 gggcctcagc cactgctcaa tggcagcata gtaccggcac cacctgccac cctagcacca
     2161 ggagtgcatc tggatgtgga ggccctcatc cccacacttg atgagcaggg ccggcccatc
     2221 ccggagtgga agcgccaggt gatggtccgg aagctgcagc agaaaatgca ggaggaagag
     2281 gagcagcgga ggaaggaaga agaggaggag gcccggctgg ccagcctgcc cgcctggaga
     2341 cgagacattc ttaggaagaa gctggaggag gagagggagc agaagcgaaa agaggaggag
     2401 cggcagaagc tggaggaaat ccagagggcg aaagaacagt cggagaagct gcggacacta
     2461 ggatacgacg aggccaagct cgcgccctgg cagcgacagg tcatcttgaa gaagggggag
     2521 atccctaagt aataggagtc tcggcctctt gcgtgtagcc tcacgagctc tgagaaatgg
     2581 ggagcggccc ccagcccccg ccccatcctg tcaggttggt gcggaagggt gggagccttg
     2641 agcccttccc ttcggtgcct gccggaatct tccctattcc cctccctggc cccacatccc
     2701 caaccctgct gctggagtgc tctcgcgaac ccctgctgtc gcctggaaaa aaaaagtgcc
     2761 caggctgctg acgcaaaaag aaaaaaagag aaagaaaaaa gatgctcatt tgcatgctca
     2821 cttacatata tttgcatgtt caccgaccca gccggagctc gccccagcct cgtgggtggt
     2881 gacttttcct gcagggcgca cgccctgctg cagccccctc ccccgcagcc cggaacccac
     2941 attgctggcg catcctgggt ggaggcagac cccgagctcg gggaaggggt tttcctccct
     3001 ccacgaccca gatctgtgct ggccaaagtt gctgcgtttc ttatcccctg cgagggtttc
     3061 ctctcagtca tttgtttacc agaaaggatg aaacttgcct gtaggggcgg agctgtcgct
     3121 cctgaggggc cccaccttta cacgtaccac cccctccccc cgccccagtt acgccttcca
     3181 agttcccagt caggccctta gcttccctga ccctttcact gcctcaatgg tataagtgga
     3241 gggccaggga tcccgcaatt gtacctgctc ctcaccctat cccccacccc cttccttccc
     3301 ccattaaacc tctatccgcc aa
//
