LOCUS       NM_001039345            1051 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Rattus norvegicus equatorin (Eqtn), mRNA.
ACCESSION   NM_001039345 XM_575866
VERSION     NM_001039345.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Hu,X.Q., Ji,S.Y., Li,Y.C., Fan,C.H., Cai,H., Yang,J.L., Zhang,C.P.,
            Chen,M., Pan,Z.F., Hu,Z.Y., Gao,F. and Liu,Y.X.
  TITLE     Acrosome formation-associated factor is involved in fertilization
  JOURNAL   Fertil Steril 93 (5), 1482-1492 (2010)
   PUBMED   19285662
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Yoshida,K., Ito,C., Yamatoya,K., Maekawa,M., Toyama,Y.,
            Suzuki-Toyota,F. and Toshimori,K.
  TITLE     A model of the acrosome reaction progression via the acrosomal
            membrane-anchored protein equatorin
  JOURNAL   Reproduction 139 (3), 533-544 (2010)
   PUBMED   20032212
REFERENCE   3  (bases 1 to 1051)
  AUTHORS   Yamatoya,K., Yoshida,K., Ito,C., Maekawa,M., Yanagida,M.,
            Takamori,K., Ogawa,H., Araki,Y., Miyado,K., Toyama,Y. and
            Toshimori,K.
  TITLE     Equatorin: identification and characterization of the epitope of
            the MN9 antibody in the mouse
  JOURNAL   Biol Reprod 81 (5), 889-897 (2009)
   PUBMED   19605790
REFERENCE   4  (bases 1 to 1051)
  AUTHORS   Li,Y.C., Hu,X.Q., Zhang,K.Y., Guo,J., Hu,Z.Y., Tao,S.X., Xiao,L.J.,
            Wang,Q.Z., Han,C.S. and Liu,Y.X.
  TITLE     Afaf, a novel vesicle membrane protein, is related to acrosome
            formation in murine testis
  JOURNAL   FEBS Lett 580 (17), 4266-4273 (2006)
   PUBMED   16831425
  REMARK    GeneRIF: Afaf expressed abundantly in the round spermatids,
            localized in the inner and outer membrane of forming acrosome, and
            declined in the maturing acrosomes.
REFERENCE   5  (bases 1 to 1051)
  AUTHORS   Toshimori,K., Saxena,D.K., Tanii,I. and Yoshinaga,K.
  TITLE     An MN9 antigenic molecule, equatorin, is required for successful
            sperm-oocyte fusion in mice
  JOURNAL   Biol Reprod 59 (1), 22-29 (1998)
   PUBMED   9674989
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from DQ336136.1.
            
            On Jan 31, 2006 this sequence version replaced XM_575866.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ336136.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA5760383, SAMEA5760389
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="5"
                     /map="5q33"
     gene            1..1051
                     /gene="Eqtn"
                     /gene_synonym="Afaf"
                     /note="equatorin"
                     /db_xref="GeneID:500502"
                     /db_xref="RGD:1563332"
     exon            1..157
                     /gene="Eqtn"
                     /gene_synonym="Afaf"
                     /inference="alignment:Splign:2.1.0"
     CDS             82..924
                     /gene="Eqtn"
                     /gene_synonym="Afaf"
                     /note="acrosome formation-associated factor; Acrosome
                     formation associated factor; equatorin, sperm acrosome
                     associated"
                     /codon_start=1
                     /product="equatorin precursor"
                     /protein_id="NP_001034434.1"
                     /db_xref="GeneID:500502"
                     /db_xref="RGD:1563332"
                     /translation="MDFILLIFLSGVFLPNIFSLQPTVEQGPGVTISDDQYYDEEENN
                     TDENSAIFQKLEDNGGDTPANEKTGNYYKDIKQYVFTTPDSKGTKTEVSVTATTDLKF
                     TMKDYKSSKATASGEEDKRSEPSRKSSTPNVPAFWTMLAKAINETAVSMDDKDLFYQA
                     IPASDLNSTNEDQLSELEEIKLKLMLGISLMTLILLIPLLIFCFATLYKLRHLRDKTC
                     ESQYSVNPELATLSYFHPSEGSNQTVLTEESSFLPPEESGKVVIIESNTVNEAEVTEE
                     RISE"
     exon            158..313
                     /gene="Eqtn"
                     /gene_synonym="Afaf"
                     /inference="alignment:Splign:2.1.0"
     exon            314..400
                     /gene="Eqtn"
                     /gene_synonym="Afaf"
                     /inference="alignment:Splign:2.1.0"
     exon            401..463
                     /gene="Eqtn"
                     /gene_synonym="Afaf"
                     /inference="alignment:Splign:2.1.0"
     exon            464..508
                     /gene="Eqtn"
                     /gene_synonym="Afaf"
                     /inference="alignment:Splign:2.1.0"
     exon            509..568
                     /gene="Eqtn"
                     /gene_synonym="Afaf"
                     /inference="alignment:Splign:2.1.0"
     exon            569..722
                     /gene="Eqtn"
                     /gene_synonym="Afaf"
                     /inference="alignment:Splign:2.1.0"
     exon            723..796
                     /gene="Eqtn"
                     /gene_synonym="Afaf"
                     /inference="alignment:Splign:2.1.0"
     exon            797..1051
                     /gene="Eqtn"
                     /gene_synonym="Afaf"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgagtcttag gaccttctgg gccacaagaa gaaagtcctg aggagacagt agattgctgg
       61 ataaatcaca ccaatttccc aatggatttt atactgttga tctttttatc tggagttttc
      121 ctcccgaaca ttttcagtct acagcccact gtggaacagg gtcctggtgt gacgatttca
      181 gatgatcagt attatgatga ggaagaaaat aatacagatg agaattctgc tattttccaa
      241 aaattagaag ataatggagg tgatactcct gccaatgaga aaactggcaa ttactataaa
      301 gacataaagc aatatgtgtt cactacacca gattcaaaag gcacgaaaac tgaagtgtct
      361 gtgacagcca caactgacct gaagtttact atgaaggact ataaatccag caaagcaact
      421 gccagtggag aagaagataa acgtagtgaa ccctctcgta aaagctcaac ccccaatgtg
      481 cctgcattct ggacaatgtt agcgaaagct ataaatgaaa cagcagtgag catggatgat
      541 aaagatctat tttatcaagc aattccagcc tctgatttaa atagtaccaa tgaagaccag
      601 ctgtcagagc tagaggaaat caagctcaaa ttaatgctgg gaatctcact gatgacactt
      661 attctcttaa ttcccctctt gatattctgt tttgccacac tgtacaaatt gagacaccta
      721 cgcgacaaaa cttgtgagag ccaatactcc gtcaacccag agctggcaac tctgtcttac
      781 ttccatccat cagaaggctc taatcaaaca gtcttaaccg aagagtcaag cttccttcct
      841 cctgaggagt cgggtaaggt ggtcatcatt gaaagtaaca cagtgaatga ggctgaagtc
      901 acggaagaaa gaatctctga gtaaataact ttaattcttt ggttataaaa acagacctgt
      961 gtttgtcaca taaatgttct ccttttgata ctgcacattt cagaaacatc cacattttct
     1021 gacatcctaa ggacattcaa taaaaactat c
//
