LOCUS       NM_001376935            4838 bp    mRNA    linear   ROD 07-MAY-2024
DEFINITION  Rattus norvegicus EGFR pathway substrate 8, signaling adaptor
            (Eps8), mRNA.
ACCESSION   NM_001376935 XM_001072957 XM_232499
VERSION     NM_001376935.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Wang,Y.T., Huang,C.C., Lin,Y.S., Huang,W.F., Yang,C.Y., Lee,C.C.,
            Yeh,C.M. and Hsu,K.S.
  TITLE     Conditional deletion of Eps8 reduces hippocampal synaptic
            plasticity and impairs cognitive function
  JOURNAL   Neuropharmacology 112 (Pt A), 113-123 (2017)
   PUBMED   27450093
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Stamatakou,E., Hoyos-Flight,M. and Salinas,P.C.
  TITLE     Wnt Signalling Promotes Actin Dynamics during Axon Remodelling
            through the Actin-Binding Protein Eps8
  JOURNAL   PLoS One 10 (8), e0134976 (2015)
   PUBMED   26252776
  REMARK    GeneRIF: These findings demonstrate a novel role for Wnt-Dvl1
            signalling through Eps8 in the regulation of axonal remodeling.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4838)
  AUTHORS   Gungor-Ordueri,N.E., Celik-Ozenci,C. and Cheng,C.Y.
  TITLE     Fascin 1 is an actin filament-bundling protein that regulates
            ectoplasmic specialization dynamics in the rat testis
  JOURNAL   Am J Physiol Endocrinol Metab 307 (9), E738-E753 (2014)
   PUBMED   25159326
  REMARK    GeneRIF: Data suggest that fascin 1 is involved in localization of
            actin-bundling proteins Eps8 and paladin during spermatogenesis;
            thus, fascin 1 regulates cytoskeletal filaments in Sertoli cells
            and in spermatid polarity.
REFERENCE   4  (bases 1 to 4838)
  AUTHORS   Behlouli,A., Bonnet,C., Abdi,S., Bouaita,A., Lelli,A.,
            Hardelin,J.P., Schietroma,C., Rous,Y., Louha,M., Cheknane,A.,
            Lebdi,H., Boudjelida,K., Makrelouf,M., Zenati,A. and Petit,C.
  TITLE     EPS8, encoding an actin-binding protein of cochlear hair cell
            stereocilia, is a new causal gene for autosomal recessive profound
            deafness
  JOURNAL   Orphanet J Rare Dis 9, 55 (2014)
   PUBMED   24741995
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4838)
  AUTHORS   Furness,D.N., Johnson,S.L., Manor,U., Ruttiger,L., Tocchetti,A.,
            Offenhauser,N., Olt,J., Goodyear,R.J., Vijayakumar,S., Dai,Y.,
            Hackney,C.M., Franz,C., Di Fiore,P.P., Masetto,S., Jones,S.M.,
            Knipper,M., Holley,M.C., Richardson,G.P., Kachar,B. and Marcotti,W.
  TITLE     Progressive hearing loss and gradual deterioration of sensory hair
            bundles in the ears of mice lacking the actin-binding protein
            Eps8L2
  JOURNAL   Proc Natl Acad Sci U S A 110 (34), 13898-13903 (2013)
   PUBMED   23918390
REFERENCE   6  (bases 1 to 4838)
  AUTHORS   Disanza,A., Mantoani,S., Hertzog,M., Gerboth,S., Frittoli,E.,
            Steffen,A., Berhoerster,K., Kreienkamp,H.J., Milanesi,F., Di
            Fiore,P.P., Ciliberto,A., Stradal,T.E. and Scita,G.
  TITLE     Regulation of cell shape by Cdc42 is mediated by the synergic
            actin-bundling activity of the Eps8-IRSp53 complex
  JOURNAL   Nat Cell Biol 8 (12), 1337-1347 (2006)
   PUBMED   17115031
REFERENCE   7  (bases 1 to 4838)
  AUTHORS   Offenhauser,N., Castelletti,D., Mapelli,L., Soppo,B.E.,
            Regondi,M.C., Rossi,P., D'Angelo,E., Frassoni,C., Amadeo,A.,
            Tocchetti,A., Pozzi,B., Disanza,A., Guarnieri,D., Betsholtz,C.,
            Scita,G., Heberlein,U. and Di Fiore,P.P.
  TITLE     Increased ethanol resistance and consumption in Eps8 knockout mice
            correlates with altered actin dynamics
  JOURNAL   Cell 127 (1), 213-226 (2006)
   PUBMED   17018287
REFERENCE   8  (bases 1 to 4838)
  AUTHORS   Disanza,A., Carlier,M.F., Stradal,T.E., Didry,D., Frittoli,E.,
            Confalonieri,S., Croce,A., Wehland,J., Di Fiore,P.P. and Scita,G.
  TITLE     Eps8 controls actin-based motility by capping the barbed ends of
            actin filaments
  JOURNAL   Nat Cell Biol 6 (12), 1180-1188 (2004)
   PUBMED   15558031
REFERENCE   9  (bases 1 to 4838)
  AUTHORS   Scita,G., Tenca,P., Areces,L.B., Tocchetti,A., Frittoli,E.,
            Giardina,G., Ponzanelli,I., Sini,P., Innocenti,M. and Di Fiore,P.P.
  TITLE     An effector region in Eps8 is responsible for the activation of the
            Rac-specific GEF activity of Sos-1 and for the proper localization
            of the Rac-based actin-polymerizing machine
  JOURNAL   J Cell Biol 154 (5), 1031-1044 (2001)
   PUBMED   11524436
REFERENCE   10 (bases 1 to 4838)
  AUTHORS   van Niel,G., Raposo,G., Candalh,C., Boussac,M., Hershberg,R.,
            Cerf-Bensussan,N. and Heyman,M.
  TITLE     Intestinal epithelial cells secrete exosome-like vesicles
  JOURNAL   Gastroenterology 121 (2), 337-349 (2001)
   PUBMED   11487543
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000004.1.
            
            On or before Dec 3, 2019 this sequence version replaced
            XM_232499.9, XM_001072957.6.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR26643288.20926.1,
                                           SRR26643287.12237.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-571               JAXUCZ010000004.1  172217590-172218160 c
            572-651             JAXUCZ010000004.1  172186497-172186576 c
            652-728             JAXUCZ010000004.1  172184812-172184888 c
            729-796             JAXUCZ010000004.1  172175952-172176019 c
            797-958             JAXUCZ010000004.1  172174805-172174966 c
            959-1108            JAXUCZ010000004.1  172174523-172174672 c
            1109-1191           JAXUCZ010000004.1  172169262-172169344 c
            1192-1328           JAXUCZ010000004.1  172168680-172168816 c
            1329-1402           JAXUCZ010000004.1  172166097-172166170 c
            1403-1529           JAXUCZ010000004.1  172163670-172163796 c
            1530-1618           JAXUCZ010000004.1  172161055-172161143 c
            1619-1693           JAXUCZ010000004.1  172160637-172160711 c
            1694-1842           JAXUCZ010000004.1  172158515-172158663 c
            1843-2026           JAXUCZ010000004.1  172154907-172155090 c
            2027-2160           JAXUCZ010000004.1  172151796-172151929 c
            2161-2269           JAXUCZ010000004.1  172147143-172147251 c
            2270-2413           JAXUCZ010000004.1  172146142-172146285 c
            2414-2636           JAXUCZ010000004.1  172132921-172133143 c
            2637-2817           JAXUCZ010000004.1  172125528-172125708 c
            2818-2947           JAXUCZ010000004.1  172124182-172124311 c
            2948-4838           JAXUCZ010000004.1  172119497-172121387 c
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="4"
                     /map="4q44"
     gene            1..4838
                     /gene="Eps8"
                     /note="EGFR pathway substrate 8, signaling adaptor"
                     /db_xref="GeneID:312812"
                     /db_xref="RGD:1310590"
     exon            1..571
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            572..651
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     CDS             593..3061
                     /gene="Eps8"
                     /note="epidermal growth factor receptor pathway substrate
                     8"
                     /codon_start=1
                     /product="epidermal growth factor receptor kinase
                     substrate 8"
                     /protein_id="NP_001363864.1"
                     /db_xref="GeneID:312812"
                     /db_xref="RGD:1310590"
                     /translation="MNGHMSNHSSGYGIYPSQMNGYGSSPPYSQMDREHCSRTSAKAL
                     YEQRKNYARDSVSSVSDVSQYRVEHLTTFVLDRKDAMITVDDGIRKLKLLDAKGKVWT
                     QDMILQVDDRAVSLIDLESKNELENFPLNTIQHCQAVAHTCSYDSILALVCKEPTQNK
                     PDLHLFQCDEVKANLISEDIESAISDSKGGKQKRRPEALRMIAKADPGIPPPPRAPAP
                     VPPGTVTQVDVRSRVAAWSAWAADQGDFEKPRQYHEQEETPEMMAARIDRDVQILNHI
                     LDDIEFFITKLQKAAEAFSELSKRKKSKKSKRKGPGEGVLTLRAKPPPPDEFVDCFQK
                     FKHGFNLLAKLKSHIQNPSASDLVHFLFTPLNMVVQATGGPELASSVLSPLLTKDTVD
                     FLNYTVKAEERQLWMSLGETWMKVRAEWPKEQFIPPYVPRFRNGWEPPMLNFMGAPTE
                     QDMYQLAESVANAAEQQRKQDSKRQSTEHSSMSDYPPADGYTFSNSMYHRGPHVDQGE
                     AALALKSTPNRHVDRNYDPVKTQPKKYAKSKYDFVARNSSELSVMKDDVLEILDDRKQ
                     WWKVRNASGDSGFVPNNILDIMRTPESGVGRTDPPYTHTIQKQRTEYGPRSADTPSAP
                     SPPPTPAPVPVPLPPSAPAPVPVPKVPANVTRQNSSSSESGGSIARDSQRYKQLPVDR
                     RKSQMEEVQDELFQRLTIGRSAAQRKFHVPRQNVPVINITYDSSPEEVKTWLQSKGFN
                     PVTVSSLGVLNGAQLFSLNKDELRSVCPEGARVFSQITVQKAALEDSSGSSELQEIMR
                     RRQEKISAAASDSGVESFDEGSSH"
     exon            652..728
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            729..796
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            797..958
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1108
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1191
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1328
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1402
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1529
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1618
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1693
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1842
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..2026
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2160
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2161..2269
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2270..2413
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2414..2636
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2637..2817
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2818..2947
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
     exon            2948..4838
                     /gene="Eps8"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tgggtgctga gaactgaatc caggtcctcc accaggccag ggaggactct cagccattag
       61 tcaaatctcc agctgccctg tacatacata ttttattgta cctgcattta cttttctgtg
      121 tttcttgtac aaagcctttc ttcgttagct catctaacca ccatattgct gcacctcccc
      181 cttggttact cattgacacc cagttcctta gactctgact cttcagcgta aaagtggcca
      241 tagtaaccac atctagcacc cagacagctc tccatcaatg cctctcattg tgctttggta
      301 ctctgtcact ctccacacct tgggcaagca ctttaccttc tcagcagtgg ctatttcctc
      361 attaaaaaga ggaggaagtc ttagctaagg ccgagagagt ccaaaggaga gactttgttg
      421 agtatagcgg aacatcgaaa ctgaagcagg gtccctccct ctcggtgtat gaaccaaggg
      481 tcactggaca cgtccgagag cagggagcgg tgctctctca gtcttgcctt gacggtcact
      541 tggaagagtt ttacctgagc actgcaggag gagcagaggc gtgagagccg gcatgaatgg
      601 tcatatgtcc aaccattcca gtggttatgg aatctaccct tctcaaatga atggttacgg
      661 atcttcccca ccctactccc agatggacag agaacactgc tccaggacaa gtgcaaaggc
      721 cctttatgaa caaaggaaga actatgcccg agacagcgtc agcagtgtgt cggacgtgtc
      781 ccagtaccga gtggaacacc tgaccacctt tgtgctggac cggaaagatg ccatgataac
      841 cgtcgatgat ggaataagga agctgaagct acttgatgcc aagggtaaag tgtggaccca
      901 ggatatgatt ctccaagtgg atgaccgagc tgtgagcctc attgacttgg aatcgaagaa
      961 cgaattggag aatttccctt taaacacaat ccagcactgt caagcagtgg cacacacatg
     1021 tagctatgat tccattctcg ccttggtatg caaagagcca acgcaaaaca agccagacct
     1081 tcaccttttc cagtgtgatg aggttaaggc aaacctaatt agcgaagata ttgaaagtgc
     1141 tatcagcgac agtaaaggcg ggaaacagaa gaggcggccg gaggccctga ggatgattgc
     1201 caaagcagat cctggcatcc ctcctcctcc cagagctcct gcccctgtgc caccagggac
     1261 tgtcacacag gtggacgtta ggagtcgagt agcagcctgg tctgcctggg cagctgacca
     1321 gggtgacttc gagaagcccc ggcagtacca cgagcaagag gagacgcccg agatgatggc
     1381 agcccggatt gacagggatg tgcaaatctt aaaccatatt ttggatgaca tcgagttttt
     1441 tatcacaaag ctccaaaaag cagctgaagc attttctgag ctttctaaaa ggaagaaaag
     1501 caagaaaagt aaaaggaaag gacctggaga gggcgtctta acactgaggg caaaaccgcc
     1561 acctcctgat gagtttgttg actgtttcca gaagtttaaa catggattca accttctggc
     1621 caagctgaag tcccacattc aaaacccgag cgcttcggat ctggttcatt ttctgtttac
     1681 tccactaaat atggtggtcc aggcaactgg tggccctgaa ctggccagtt cagtactcag
     1741 cccactgttg acgaaagaca cagttgattt cttaaattac acggtcaagg cagaggaacg
     1801 gcagctgtgg atgtcactgg gagagacgtg gatgaaagtg agagcagagt ggccgaaaga
     1861 acagttcatc ccaccttacg ttccgaggtt ccgaaacggc tgggagcccc cgatgctgaa
     1921 cttcatgggc gcacccacag agcaagacat gtatcagctg gcagagtccg tggccaacgc
     1981 agcggaacag cagcgcaaac aggacagcaa gagacagtcc acagagcatt ccagcatgtc
     2041 cgactatcct ccagccgatg gctatacgtt cagtaacagc atgtaccaca gaggaccaca
     2101 tgtggaccaa ggggaggctg ccctggctct caagtcaacc cctaatcgcc atgtagatag
     2161 aaattatgac ccggtcaaaa cacaacccaa gaaatacgcc aaatccaagt acgactttgt
     2221 ggcgaggaac agcagcgagc tctcagttat gaaggatgat gtcttagaga tactcgatga
     2281 tagaaagcag tggtggaaag tccggaatgc cagcggagac tctgggttcg tgccaaataa
     2341 cattctggat atcatgagaa ctccggaatc tggagtgggg cgcactgatc ccccatacac
     2401 acacaccata cagaaacaaa ggacagaata cggtccaaga tcagctgata ccccttctgc
     2461 cccatcgccc cctccaacac cagcacccgt tccggtcccc cttccacctt ctgcaccagc
     2521 acccgttcct gtgcccaagg ttccagcgaa tgtcacccgt cagaacagca gctccagcga
     2581 gagtgggggc agcatcgcaa gggacagcca gagatacaaa caactcccag tggaccgaag
     2641 gaaatctcag atggaggagg tgcaggatga actcttccag aggctgacca tcgggcgcag
     2701 cgccgcgcag aggaagttcc acgtgccacg gcagaacgtt ccagtgatca atatcacgta
     2761 tgactcctcc ccggaagaag tgaagacgtg gctacagtcg aaaggattca accctgtgac
     2821 ggtcagtagc ctcggggtgt tgaacggagc gcaactcttt tctctcaaca aagacgagct
     2881 gaggtctgtc tgccctgaag gtgccagagt gttcagtcaa atcaccgtcc agaaagccgc
     2941 tctggaggac agcagcggaa gctctgagtt acaagaaatc atgcggagaa ggcaggagaa
     3001 gatcagcgct gcggccagcg actcgggagt ggagtccttc gatgaaggga gcagccactg
     3061 atttccttga actccgttat ccttggcacg gctgttggat ggtgttggac actctttgtt
     3121 ttaagaagcc ttgaagggaa tgtcaaatct attgtcttgg tatatgtaat ccatctccat
     3181 aaaaggagac cgtataatgc ctgagtaagc agaggacttg ctgcttctgc gcccactgtt
     3241 ttgattaaaa ctgagaagtg ggcaggtgag agggctcagc aagtagaggt gcttgctgcc
     3301 aaacacagag acctgagttc gagtcccccg ggcctacatg gtagaaggcg agagctagct
     3361 tctgcaagtt gtcctttgac caccacacat agataaataa caaatgtaat ttaaaaaaaa
     3421 attttaaaga aagtttaatt taagaaaaaa aaaacaacag aagttttaga ctgttctcag
     3481 gttgggaaat atttttttcc cactttccta aggtgtttcc ccctttgcta cgttaattat
     3541 cacagccttg cttgatgatc tgagtggatt tgacagtggc aaatgcattc actacaacag
     3601 ggaaagacac agccagcgat gaaaacggtc tcagctttca gtgactgggg tgcctgtcat
     3661 ccagggagga ggctcagtta gactaccgtc agcccacgtg aggtctgtcc tctgacatac
     3721 ctaacggggg cgtatttatc attatttaaa gttcttaatg ccaacagttt ttaagaaatg
     3781 atcacatttg aatgaactgt acagtaggac actatactct gtccccgaga aactttcttc
     3841 ccctcagctc tgttgattgc gaaatcctgc atagattatg ctttgatgta gtttctgctt
     3901 attacgtcaa ctgtaattaa acaccagaaa gcacaagttc aaagtaaggc atatgcattc
     3961 acaatcagag tagcccacct tggtgtttat tacagtattt cgcttagcga aggccccagt
     4021 tataaatatt gacataagta gtcaaaggat attgggccat tgtatgtgtt ttacctgtgt
     4081 atcagggaca cttaatttac gtgtactcta gccccacgga agagtctaca tggagagaaa
     4141 gcacatcgct aaccttcaac aaatgttatt tttggaaaca caggtttttg tcattaaaca
     4201 taatgttatt tcacatatac aaaggagatg ttatgtaaga atgttgtata ttttaacata
     4261 aatccattta gagagattat ctagattcat taatttttca tagtgccttt ttcacatgag
     4321 tcagctggag agagcctgca ataaacagta tttgctgtgt attaataaat agctcctatc
     4381 tcgtcaacac gggaacgtgt gctttgtagg ctgagcgttt ggggtgggcg ggatgaagga
     4441 cgtcccctta gaaaggaggc tccagggtgt gtctgtgctg acagtggcct aggatactgc
     4501 cccgtgttcc cctgctttct gcctcgcttg gtttctcatc cctgccttct accatcaatt
     4561 cggagttgga aaccagagtg gcagctcaca caggctgcat ttgttattga taaacgctcc
     4621 aaactgaata cgtttctgga gatctgcagt aaccgccagc aacttcccgg ggccatgttt
     4681 ccagacgtcg acgaagggca cggcagcctt tcctttcgac ttctgtcttc ccatcatcct
     4741 tagcacatgt aagacgagaa agaatgatgc cttgtgttct gtgaacccct cattgaaaaa
     4801 aaaaggctct gaaaggcaaa ataaaattca aatattaa
//
