LOCUS       NM_001024238            4461 bp    mRNA    linear   ROD 24-APR-2024
DEFINITION  Rattus norvegicus glutamyl-prolyl-tRNA synthetase 1 (Eprs1),
            transcript variant 1, mRNA.
ACCESSION   NM_001024238 XM_213969
VERSION     NM_001024238.1
KEYWORDS    RefSeq.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Arif,A., Terenzi,F., Potdar,A.A., Jia,J., Sacks,J., China,A.,
            Halawani,D., Vasu,K., Li,X., Brown,J.M., Chen,J., Kozma,S.C.,
            Thomas,G. and Fox,P.L.
  TITLE     EPRS is a critical mTORC1-S6K1 effector that influences adiposity
            in mice
  JOURNAL   Nature 542 (7641), 357-361 (2017)
   PUBMED   28178239
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Forster,F., Paster,W., Supper,V., Schatzlmaier,P., Sunzenauer,S.,
            Ostler,N., Saliba,A., Eckerstorfer,P., Britzen-Laurent,N.,
            Schutz,G., Schmid,J.A., Zlabinger,G.J., Naschberger,E., Sturzl,M.
            and Stockinger,H.
  TITLE     Guanylate binding protein 1-mediated interaction of T cell antigen
            receptor signaling with the cytoskeleton
  JOURNAL   J Immunol 192 (2), 771-781 (2014)
   PUBMED   24337748
REFERENCE   3  (bases 1 to 4461)
  AUTHORS   Son,J., Lee,E.H., Park,M., Kim,J.H., Kim,J., Kim,S., Jeon,Y.H. and
            Hwang,K.Y.
  TITLE     Conformational changes in human prolyl-tRNA synthetase upon binding
            of the substrates proline and ATP and the inhibitor halofuginone
  JOURNAL   Acta Crystallogr D Biol Crystallogr 69 (Pt 10), 2136-2145 (2013)
   PUBMED   24100331
REFERENCE   4  (bases 1 to 4461)
  AUTHORS   Zhou,H., Sun,L., Yang,X.L. and Schimmel,P.
  TITLE     ATP-directed capture of bioactive herbal-based medicine on human
            tRNA synthetase
  JOURNAL   Nature 494 (7435), 121-124 (2013)
   PUBMED   23263184
REFERENCE   5  (bases 1 to 4461)
  AUTHORS   Arif,A., Chatterjee,P., Moodt,R.A. and Fox,P.L.
  TITLE     Heterotrimeric GAIT complex drives transcript-selective translation
            inhibition in murine macrophages
  JOURNAL   Mol Cell Biol 32 (24), 5046-5055 (2012)
   PUBMED   23071094
REFERENCE   6  (bases 1 to 4461)
  AUTHORS   Kaminska,M., Havrylenko,S., Decottignies,P., Le Marechal,P.,
            Negrutskii,B. and Mirande,M.
  TITLE     Dynamic Organization of Aminoacyl-tRNA Synthetase Complexes in the
            Cytoplasm of Human Cells
  JOURNAL   J Biol Chem 284 (20), 13746-13754 (2009)
   PUBMED   19289464
REFERENCE   7  (bases 1 to 4461)
  AUTHORS   Kaminska,M., Havrylenko,S., Decottignies,P., Gillet,S., Le
            Marechal,P., Negrutskii,B. and Mirande,M.
  TITLE     Dissection of the structural organization of the aminoacyl-tRNA
            synthetase complex
  JOURNAL   J Biol Chem 284 (10), 6053-6060 (2009)
   PUBMED   19131329
REFERENCE   8  (bases 1 to 4461)
  AUTHORS   Sampath,P., Mazumder,B., Seshadri,V., Gerber,C.A., Chavatte,L.,
            Kinter,M., Ting,S.M., Dignam,J.D., Kim,S., Driscoll,D.M. and
            Fox,P.L.
  TITLE     Noncanonical function of glutamyl-prolyl-tRNA synthetase:
            gene-specific silencing of translation
  JOURNAL   Cell 119 (2), 195-208 (2004)
   PUBMED   15479637
REFERENCE   9  (bases 1 to 4461)
  AUTHORS   Kim,J.Y., Kang,Y.S., Lee,J.W., Kim,H.J., Ahn,Y.H., Park,H., Ko,Y.G.
            and Kim,S.
  TITLE     p38 is essential for the assembly and stability of macromolecular
            tRNA synthetase complex: implications for its physiological
            significance
  JOURNAL   Proc Natl Acad Sci U S A 99 (12), 7912-7916 (2002)
   PUBMED   12060739
REFERENCE   10 (bases 1 to 4461)
  AUTHORS   Ko,Y.G., Kang,Y.S., Kim,E.K., Park,S.G. and Kim,S.
  TITLE     Nucleolar localization of human methionyl-tRNA synthetase and its
            role in ribosomal RNA synthesis
  JOURNAL   J Cell Biol 149 (3), 567-574 (2000)
   PUBMED   10791971
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY383705.1.
            
            On May 30, 2005 this sequence version replaced XM_213969.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY383705.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN13663622,
                                           SAMN13663629 [ECO:0000350]
            ##Evidence-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="Sprague-Dawley"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q26"
     gene            1..4461
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /note="glutamyl-prolyl-tRNA synthetase 1"
                     /db_xref="GeneID:289352"
                     /db_xref="RGD:1310952"
     CDS             1..4461
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /note="isoform 1 is encoded by transcript variant 1;
                     bifunctional aminoacyl-tRNA synthetase; bifunctional
                     glutamate/proline--tRNA ligase; proline--tRNA ligase-like"
                     /codon_start=1
                     /product="bifunctional glutamate/proline--tRNA ligase
                     isoform 1"
                     /protein_id="NP_001019409.1"
                     /db_xref="GeneID:289352"
                     /db_xref="RGD:1310952"
                     /translation="MAALCLTVNAGNPPLGCRVKVNKRNKSFMLSFLEALLAVEHVKG
                     DVSISVEEGKENLLRVSESVVFTDTNSILRYLARVAATSGLYGSNLMEHTEIDHWLEF
                     SATKLSSCDVLTSAIKELNHCLSLRTYLVGNSLTLADLCVWATLKGNAACQEHLRQSK
                     TLVHVNRWYGFLEAQQAFRSVGTKWDASANKATGAPDRKQDIGKFVELPGAEMGKVTV
                     RFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAM
                     LHIKPDQFTYTSDHFETIMKYAEKLIEEGKAYVDDTPADEMKAEPVEKNLQMWEEMKR
                     GSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDS
                     IEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNE
                     GLGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVIPVNILDAQEEMKEVARHP
                     KNPDVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNINITKIHKNPDGKITSLDA
                     KLNLENKDYKKTTKITWLAETTQALPVPAVCVTYEHLITKPVLGKDEDFKQYVNKDSK
                     HEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCILIYIPDGHTK
                     EMPTSGSKEKTKVEISKKETGSAPKERPAAAVSAPCAAAEDAAILYSRVAVQGDAVRD
                     LKAKKAPKEDIDAAVKQLLTLKAEYKEKTGQEYRPGNPPTAAVQTVSTKSPSNTGEYT
                     SLYNKVAAQGELVRKLKAEKAPKAKINDAVECLLSLKAEYKEKTGKEYVPGQLPASQN
                     SHSSPVSNAKPVGSETPEAKLLFDRVACQGEVVRKLKAEKASKDQVDSAVQELLQLKA
                     QYKSLTGIEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQGKDSSKSQGSGLS
                     SGGAGEGQGPKKQTRLGLEAKKEENLAEWYSQVITKSEMIEYYDVSGCYILRPWSYSI
                     WESIKDFFDAEIKKLGVENCYFPIFVSQGALEKEKNHIADFAPEVAWVTRSGKTELAE
                     PIAIRPTSETVMYPAYAKWVQSHRDLPIRLNQWCNVVRWEFKHPQPFLRTREFLWQEG
                     HSAFATFEEAADEVLQILELYARVYEELLAIPVVRGRKTEKEKFAGGDYTTTLEAFIS
                     ASGRAIQGATSHHLGQNFSKMCEIVFEDPKTPGEKQFAYQCSWGLTTRTIGVMIMVHG
                     DNMGLVLPPRVASVQVVVIPCGITNTLSEEDREALMAKCNEYRRRLLGADIRARVDLR
                     DNYSPGWKFNHWELKGVPVRLEVGPRDMKSCQFVAVRRDTGEKLTIAEKEAESKLKGV
                     LEDIQLNLFTRASEDLKSHMVVSDTLEDFQKVLDSGKVAQIPFCGEIDCEDWIKKTTA
                     RDQDVEPGAPSMGAKSLCIPFNPLCELQPGAMCVCGKNPAKFYTLFGRSY"
     exon            1..46
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            47..69
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            70..185
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            186..285
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            286..442
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            443..582
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            583..677
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            678..804
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            805..961
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1133
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1356
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1416
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1527
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1664
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1872
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..1985
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            1986..2103
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2463
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            2464..2697
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2960
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            2961..3012
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3013..3222
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3223..3295
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3296..3375
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3376..3477
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3478..3633
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3634..3831
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            3832..4005
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            4006..4166
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            4167..4245
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            4246..4310
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
     exon            4311..4461
                     /gene="Eprs1"
                     /gene_synonym="Eprs"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atggcggcgc tgtgccttac ggtgaacgcc ggaaaccctc cactgggttg tcgagttaaa
       61 gtgaacaaga gaaataaatc cttcatgttg tcatttttag aagctctgct ggcagtggag
      121 catgtgaaag gtgatgtcag catttctgtg gaagaaggga aggagaatct tcttcgggtt
      181 tctgagagtg tggtgttcac tgacacaaat tcaatcctgc gctacttggc tagagttgca
      241 gctacatctg ggctgtacgg ttctaacctg atggagcaca cggagattga tcattggttg
      301 gagttcagcg ctacaaagtt gtcttcgtgt gatgtgctga cttcggcaat caaggagctt
      361 aaccattgcc tgtctctgag aacatacctg gttgggaact ctttgacttt agcagattta
      421 tgtgtttggg ccaccctaaa aggaaatgct gcatgtcagg agcacttgag acagagcaaa
      481 accctagtcc acgttaaccg ctggtatggc tttcttgaag cccagcaggc cttccgttca
      541 gttggtacca agtgggatgc ttcagcaaac aaagctacag gggcacctga cagaaaacaa
      601 gatattggga aattcgtcga gcttccgggt gcagagatgg gaaaggtgac agtcagattc
      661 cccccagagg ctagtggtta cttacacatt gggcatgcaa aggctgctct tctgaaccag
      721 cactaccagg ttaactttaa aggcaaactg atcatgagat ttgatgacac aaacccagaa
      781 aaggaaaagg aagattttga gaaggtcatc ttggaagatg ttgcaatgct gcacattaaa
      841 ccagatcagt ttacttacac ttcagatcat tttgaaacta taatgaagta tgcagagaaa
      901 ttaattgaag aagggaaggc ctatgtggac gacactcccg cagacgagat gaaggcggag
      961 cctgttgaga agaatctaca aatgtgggaa gaaatgaaaa gaggaagcca gtttggtcag
     1021 tcctgttgcc tgcgagcaaa aattgacatg agtagtaaca atgggtgtat gagggaccca
     1081 accctttacc gctgcaaaat ccagccccac ccaagaactg ggaataaata caatgtttac
     1141 ccaacgtatg actttgcctg tcctatagtg gacagcattg aaggtgttac tcatgccttg
     1201 aggacgacag agtaccacga ccgagatgag cagttttact ggattattga agccttaggc
     1261 ataaggaaac catacatctg ggaatacagt cgcttaaatc tcaacaacac agtgctgtcc
     1321 aagagaaagc ttacgtggtt tgtcaatgaa ggactgggct cctcaaggtc tgttgtaaac
     1381 atggaatggg acaaaatctg ggcgtttaac aaaaaggtta ttgaccctgt ggctccaagg
     1441 tacgttgcat tgctgaagaa ggaggtgatc ccagtgaaca tcctggacgc gcaggaggag
     1501 atgaaggaag tagccagaca tcccaagaat cctgatgttg gtttgaagcc tgtgtggtat
     1561 agtcccaagg ttttcataga aggagctgat gcagagactt tctcagaggg tgaaatggtc
     1621 acatttataa actggggcaa catcaacatt actaaaatac acaaaaatcc agatggaaaa
     1681 attacatctc tggatgcaaa attgaatttg gaaaacaaag actacaagaa aacaactaag
     1741 atcacttggc ttgcggagac cacacaggct ctccctgtcc cggcagtctg tgtcacttat
     1801 gagcacttga ttacaaagcc agtgctagga aaagacgagg acttcaagca gtacgtcaac
     1861 aaagacagta agcatgaaga actaatgcta ggagatccct gccttaagga tttgaagaaa
     1921 ggagacatca tacaattgca gaggcgcgga ttcttcatat gcgaccagcc gtacgaacct
     1981 gtcagcccat atagttgcaa agaagccccc tgcatcttga tatatatacc cgatgggcac
     2041 accaaggaga tgccaacatc tggatcaaag gaaaagacta aagtggaaat ctcaaaaaag
     2101 gagaccggct ctgctcctaa ggagagaccc gcagctgctg tgagtgctcc ctgtgctgca
     2161 gctgaggacg ctgccatcct ctacagcaga gtggcggtcc agggtgatgc ggttcgcgac
     2221 ctgaaagcga agaaagcacc aaaggaggat attgacgcag ctgtgaaaca gcttttgact
     2281 ttgaaggctg agtataaaga gaaaactggg caggagtata gacctgggaa cccccctact
     2341 gcagcagtgc agactgtttc tacaaagtcc ccatccaaca ccggggaata cacatcttta
     2401 tacaataaag ttgctgccca aggggagctg gtccggaagc tgaaagctga aaaggctcct
     2461 aaggctaaaa taaatgatgc tgtagaatgc ttactgtccc tgaaagctga gtataaagaa
     2521 aaaactggga aggagtatgt acctggccaa ctcccagcat ctcagaactc acattcaagt
     2581 cctgtcagca atgccaagcc tgttggatca gagacaccgg aagccaaact gctgtttgac
     2641 agagtggctt gtcaaggaga agtagttcgg aaacttaaag ctgagaaggc ctctaaggat
     2701 caagtggatt cagccgtaca agaacttctt cagctgaagg cacagtacaa gtcactgaca
     2761 ggaattgagt ataagcctgt gtctgctact ggggcagaag acaaagataa gaagaagaaa
     2821 gaaaaagaaa ataagtctga aaagcagaat aagcctcaga aacaaaatga tggccaaggg
     2881 aaagactctt ctaaaagcca aggcagcggg ctgtcgtccg gtggagcagg agaagggcaa
     2941 gggcccaaga aacagaccag attgggtctt gaagcaaaaa aagaagaaaa tcttgccgaa
     3001 tggtattccc aagtcatcac gaagtcagag atgatcgaat actacgatgt gagtggctgc
     3061 tatattctcc gaccctggtc gtactccatc tgggaatcca ttaaggactt ctttgacgcg
     3121 gagatcaaga agctcggcgt tgagaactgc tacttcccca tattcgtgtc ccagggcgca
     3181 ctagagaagg agaagaacca cattgctgac tttgctcccg aggttgcttg ggtgacaaga
     3241 tctggaaaga cagagctggc agagccgatt gccatccgtc ctactagtga aacagtaatg
     3301 tatcctgcat atgcaaaatg ggtgcagtcc cacagagacc tgcccatcag gctcaaccag
     3361 tggtgcaacg tggtgcgctg ggagttcaaa cacccacaac ctttcctccg cactcgggag
     3421 ttcctatggc aggaggggca cagcgccttt gccacctttg aggaggcagc tgacgaggtg
     3481 ttacagatcc tcgagttata tgctcgagtg tatgaggagc tcttggcaat ccccgtcgtg
     3541 agaggaagga agactgagaa ggaaaagttt gcaggaggag actatacaac cacactagaa
     3601 gccttcatat ctgccagcgg acgcgctatc cagggagcaa catcacacca tttaggccag
     3661 aatttctcca aaatgtgtga aatagttttt gaagatccca agacaccagg agaaaagcag
     3721 tttgcatacc agtgctcctg gggcctgaca actcgcacta tcggtgtgat gatcatggtc
     3781 catggggaca acatgggctt agtgttacct ccccgtgtag cgtctgtcca ggtggtagtc
     3841 attccctgtg gaatcacgaa taccttgtct gaagaagaca gagaggccct gatggcaaaa
     3901 tgcaatgagt acaggagacg gctgctcggc gctgacatcc gagctcgagt cgatctacga
     3961 gacaactact caccaggctg gaagttcaac cactgggagc tcaagggtgt tccagtcaga
     4021 cttgaggtgg ggccacgtga tatgaagagc tgtcagtttg tagcagtcag acgagatact
     4081 ggagagaagc tgacaattgc tgaaaaggag gcggagtcta aactgaaagg ggttttggaa
     4141 gacatccagc ttaacctttt cacaagggct tctgaagacc ttaaatctca catggttgta
     4201 tcggatacac tggaagactt tcagaaggtg ctagattctg ggaaggttgc acagattcca
     4261 ttctgtgggg aaattgactg tgaggactgg atcaaaaaga caacggccag ggatcaggat
     4321 gtggaacctg gtgctccatc catgggagcc aaaagccttt gcattccttt caaccctctg
     4381 tgtgagctgc agccaggagc catgtgtgtc tgtggcaaga atcctgccaa gttctacacc
     4441 ttgtttggtc ggagttactg a
//
